Restriction map for
pFN21AB6345
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 289 GGCGCC G[GA]^CG[CT]C
AlwNI 172 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 625 GGGCCC GGGCC^C
Asp700I 588 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 15 GTGCTC G[AT]GC[AT]^C
AvaI 338 CCCGGG C^[CT]CG[GA]G
BbeI 289 GGCGCC GGCGC^C
BglI 467 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 242 AGATCT A^GATCT
BsaHI 289 GGCGCC G[GA]^CG[CT]C
BseRI 748 GAGGAG GAGGAG(10/8)
BsiHKAI 15 GTGCTC G[AT]GC[AT]^C
BsmFI 180 GGGAC GGGAC(10/14)
BsoBI 338 CCCGGG C^[CT]CG[GA]G
BspHI 584 TCATGA T^CATGA
BspMI 668 ACCTGC ACCTGC(4/8)
BsrDI 294 CATTGC GCAATG(2/0)
BstYI 242 AGATCT [GA]^GATC[CT]
Hin1I 289 GGCGCC G[GA]^CG[CT]C
HincII 355 GTCAAC GT[CT]^[GA]AC
HindII 355 GTCAAC GT[CT]^[GA]AC
KasI 289 GGCGCC G^GCGCC
MaeIII 505 GTCAC ^GT[ACGT]AC
MflI 242 AGATCT [GA]^GATC[CT]
MslI 393 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 289 GGCGCC GG^CGCC
PleI 451 GACTC GAGTC(4/5)
PpuMI 180 GGGACCC [GA]G^G[AT]CC[CT]
PstI 127 CTGCAG CTGCA^G
RcaI 584 TCATGA T^CATGA
SfcI 127 CTGCAG C^T[GA][CT]AG
SfoI 289 GGCGCC GGC^GCC
SmaI 338 CCCGGG CCC^GGG
Tsp45I 505 GTCAC ^GT[GC]AC
XcmI 155 CCATAGGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 242 AGATCT [GA]^GATC[CT]
XmaCI 338 CCCGGG C^CCGGG
XmaI 338 CCCGGG C^CCGGG
XmnI 588 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 135 CGCG CG^CG
360 CGCG
AvaII 181 GGACC G^G[AT]CC
642 GGTCC
BanI 289 GGCGCC G^G[CT][GA]CC
324 GGCACC
BbsI 771 GAAGAC GAAGAC(2/6)
73 GTCTTC
BpuAI 771 GAAGAC GAAGAC(2/6)
73 GTCTTC
BsaAI 268 TACGTG [CT]AC^GT[GA]
723 CACGTA
BstUI 135 CGCG CG^CG
360 CGCG
DdeI 688 CTGAG C^T[ACGT]AG
694 CTGAG
DpnII 132 GATC ^GATC
243 GATC
EarI 673 CTCTTC CTCTTC(1/4)
760 GAAGAG
EcoT14I 392 CCATGG C^C[AT][AT]GG
776 CCATGG
HaeII 111 GGCGCT [GA]GCGC^[CT]
289 GGCGCC
HphI 215 TCACC GGTGA(8/7)
506 TCACC
Ksp632I 673 CTCTTC CTCTTC(1/4)
760 GAAGAG
MaeII 269 ACGT A^CGT
724 ACGT
MboI 132 GATC ^GATC
243 GATC
MvnI 135 CGCG CG^CG
360 CGCG
NcoI 392 CCATGG C^CATGG
776 CCATGG
NdeII 132 GATC ^GATC
243 GATC
PvuII 280 CAGCTG CAG^CTG
421 CAGCTG
SapI 760 GAAGAGC GCTCTTC(1/4)
672 GCTCTTC
Sau3AI 132 GATC ^GATC
243 GATC
SfaNI 308 GCATC GCATC(5/9)
434 GCATC
SmlI 31 CTCAAG C^T[CT][GA]AG
97 CTCAAG
StyI 392 CCATGG C^C[AT][AT]GG
776 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI BalI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PflMI Sau96I ScrFI TfiI
TseI TspRI Van91I