(Circular) (Six-base) MAPSORT of: pFN21AE0482.seq Check: 6348 from: 1 to: 5698 pFN21AE0482 5698 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2862 3041 1937 Size: 925 179 4594 Fragments arranged by size: 4594 925 179 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2052 2192 2562 2808 3761 5145 5413 1632 Size: 420 140 370 246 953 1384 268 1917 Fragments arranged by size: 1917 1384 953 420 370 268 246 140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2340 2480 2540 2935 2977 3441 5013 2340 Size: 140 60 395 42 464 1572 3025 Fragments arranged by size: 3025 1572 464 395 140 60 42 ApaI G_GGCC'C Cuts at: 2707 2707 Size: 5698 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2781 3023 1870 Size: 73 838 242 4545 Fragments arranged by size: 4545 838 242 73 BamHI G'GATC_C Cuts at: 2805 3028 3395 2805 Size: 223 367 5108 Fragments arranged by size: 5108 367 223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2416 2774 3019 4474 618 Size: 325 217 627 629 358 245 1455 1842 Fragments arranged by size: 1842 1455 629 627 358 325 245 217 BanII G_rGCy'C Cuts at: 1391 2707 2803 2958 3017 1391 Size: 1316 96 155 59 4072 Fragments arranged by size: 4072 1316 155 96 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BbvCI CC'TCA_GC Cuts at: 2907 2915 2907 Size: 8 5690 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2964 5693 2964 Size: 2729 2969 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 2308 2707 3759 4915 1790 Size: 518 399 1052 1156 2573 Fragments arranged by size: 2573 1156 1052 518 399 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 2499 3429 Size: 416 53 83 399 24 646 436 930 Cuts at: 3429 4387 442 Size: 958 1753 Fragments arranged by size: 1753 958 930 646 436 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2957 4358 1239 Size: 1718 1401 2579 Fragments arranged by size: 2579 1718 1401 Bpu10I CC'TnA_GC Cuts at: 2907 2915 2928 2907 Size: 8 13 5677 Fragments arranged by size: 5677 13 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5473 Fragments arranged by size: 5473 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2811 2924 2954 1186 Size: 1625 113 30 3930 Fragments arranged by size: 3930 1625 113 30 BseYI C'CCAG_C Cuts at: 2456 2467 4901 5397 2456 Size: 11 2434 496 2757 Fragments arranged by size: 2757 2434 496 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2326 1163 Size: 573 498 92 4535 Fragments arranged by size: 4535 573 498 92 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 2958 3759 3844 4915 2517 Size: 441 801 85 1071 3300 Fragments arranged by size: 3300 1071 801 441 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2308 2517 2707 2803 2958 3017 3759 Size: 399 518 209 190 96 155 59 742 Cuts at: 3759 3844 4915 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 518 399 209 190 155 96 85 59 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3053 844 Size: 2209 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 2086 2480 2897 794 Size: 1292 394 417 3595 Fragments arranged by size: 3595 1292 417 394 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2509 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2277 2805 2964 3028 3395 3780 3797 5249 Size: 305 528 159 64 367 385 17 1452 Cuts at: 5249 5335 5347 5693 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 528 385 367 346 305 159 86 64 17 12 BstZ17I GTA'TAC Cuts at: 2863 2863 Size: 5698 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 2793 3214 4008 4028 2793 Size: 421 794 20 4463 Fragments arranged by size: 4463 794 421 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663 Size: 54 1425 50 275 18 1315 889 1629 Cuts at: 5663 8 Size: 43 Fragments arranged by size: 1629 1425 1315 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2105 2319 3628 1353 Size: 675 77 214 1309 3423 Fragments arranged by size: 3423 1309 675 214 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2956 2956 Size: 5698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2192 2562 2808 2957 3761 4358 Size: 393 420 140 370 246 149 804 597 Cuts at: 4358 5145 5413 1239 Size: 787 268 1524 Fragments arranged by size: 1524 804 787 597 420 393 370 268 246 149 140 EcoO109I rG'GnC_Cy Cuts at: 2703 2703 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2041 2041 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2379 4845 1678 Size: 701 2466 2531 Fragments arranged by size: 2531 2466 701 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2790 2822 4339 4371 4413 Size: 32 135 32 1218 32 1517 32 42 Cuts at: 4413 4445 1373 Size: 32 2626 Fragments arranged by size: 2626 1517 1218 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2854 3042 3288 669 Size: 988 281 916 188 246 3079 Fragments arranged by size: 3079 988 916 281 246 188 HindIII A'AGCT_T Cuts at: 748 2177 2297 748 Size: 1429 120 4149 Fragments arranged by size: 4149 1429 120 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 4812 4996 1305 Size: 1071 2436 184 2007 Fragments arranged by size: 2436 2007 1071 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2880 3656 4015 4174 518 Size: 648 1714 776 359 159 2042 Fragments arranged by size: 2042 1714 776 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2456 2738 3114 3791 4939 5184 1606 Size: 850 282 376 677 1148 245 2120 Fragments arranged by size: 2120 1148 850 677 376 282 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 NdeI CA'TA_TG Cuts at: 387 2188 387 Size: 1801 3897 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 3056 4601 1132 Size: 97 1827 1545 2229 Fragments arranged by size: 2229 1827 1545 97 PasI CC'CwG_GG Cuts at: 1348 2139 1348 Size: 791 4907 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PfoI T'CCnGG_A Cuts at: 2052 2280 2052 Size: 228 5470 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PspOMI G'GGCC_C Cuts at: 2703 2703 Size: 5698 PstI C_TGCA'G Cuts at: 830 2455 3050 830 Size: 1625 595 3478 Fragments arranged by size: 3478 1625 595 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2456 2738 2456 Size: 282 5416 SacI G_AGCT'C Cuts at: 2958 2958 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2451 3046 4181 4862 5053 826 Size: 219 1406 595 1135 681 191 1471 Fragments arranged by size: 1471 1406 1135 681 595 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 2783 3025 1872 Size: 911 242 4545 Fragments arranged by size: 4545 911 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 3137 513 Size: 552 939 1133 3074 Fragments arranged by size: 3074 1133 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2589 2629 3725 3742 3895 4080 4525 Size: 1255 1009 40 1096 17 153 185 445 Cuts at: 4525 325 Size: 1498 Fragments arranged by size: 1498 1255 1096 1009 445 185 153 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2797 2829 5658 5690 2797 Size: 32 2829 32 2805 Fragments arranged by size: 2829 2805 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2353 2506 2353 Size: 153 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 2781 3023 1870 Size: 911 242 4545 Fragments arranged by size: 4545 911 242 XmnI GAAnn'nnTTC Cuts at: 2114 2298 3827 2114 Size: 184 1529 3985 Fragments arranged by size: 3985 1529 184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI