Restriction map for
pFN21AE0435
(Circular) (Six-base) MAPSORT of: pFN21AE0435.seq Check: 2370 from: 1 to: 5476
pFN21AE0435 5476 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2601 2601
Size: 5476
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AfeI AGC'GCT
Cuts at: 2768 2768
Size: 5476
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 3219 4791 3219
Size: 1572 3904
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2801 1870
Size: 73 858 4545
Fragments arranged by size:
4545 858 73
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 2797 4252 618
Size: 325 217 627 888 122 1455 1842
Fragments arranged by size:
1842 1455 888 627 325 217 122
BanII G_rGCy'C
Cuts at: 1391 2795 1391
Size: 1404 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2062 928
Size: 971 163 4342
Fragments arranged by size:
4342 971 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 2678 3537 4693 1790
Size: 888 859 1156 2573
Fragments arranged by size:
2573 1156 888 859
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2755 4136 1239
Size: 1516 1381 2579
Fragments arranged by size:
2579 1516 1381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4139 882
Size: 617 2640 2219
Fragments arranged by size:
2640 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2645 3283 3665 4300
Size: 53 83 186 936 1112 638 382 635
Cuts at: 4300 275
Size: 1451
Fragments arranged by size:
1451 1112 936 638 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 2753 2783 1178
Size: 30 165 30 817 30 503 30 3871
Fragments arranged by size:
3871 817 503 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2451 1186
Size: 1265 4211
BseYI C'CCAG_C
Cuts at: 4679 5175 4679
Size: 496 4980
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4903
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2926 3687 4715 1540
Size: 275 186 15 910 761 1028 2301
Fragments arranged by size:
2301 1028 910 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2670 3537 3622 4693 2670
Size: 867 85 1071 3453
Fragments arranged by size:
3453 1071 867 85
BsmI GAATG_Cn'
Cuts at: 2445 2684 2987 3080 2445
Size: 239 303 93 4841
Fragments arranged by size:
4841 303 239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2670 2678 2795 3537 3622 4693 1391
Size: 399 880 8 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 880 742 399 117 85 8
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 2382 2487 3365 5095 2382
Size: 105 878 1730 2763
Fragments arranged by size:
2763 1730 878 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 2831 844
Size: 1757 230 3489
Fragments arranged by size:
3489 1757 230
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 1394
Size: 429 1540 3507
Fragments arranged by size:
3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 3971 4145 65
Size: 1183 789 1934 174 1396
Fragments arranged by size:
1934 1396 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471
Size: 834 367 385 17 1452 86 12 346
Cuts at: 5471 1972
Size: 1977
Fragments arranged by size:
1977 1452 834 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4924
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2062 2095 487
Size: 683 56 836 33 3868
Fragments arranged by size:
3868 836 683 56 33
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2923 3812
Size: 54 1425 50 275 18 183 910 889
Cuts at: 3812 5441 8
Size: 1629 43
Fragments arranged by size:
1629 1425 910 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2923 1537
Size: 275 201 910 4090
Fragments arranged by size:
4090 910 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2303 3406 1353
Size: 950 1103 3423
Fragments arranged by size:
3423 1103 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2755 3539 4136 4923 5191 1239
Size: 393 1123 784 597 787 268 1524
Fragments arranged by size:
1524 1123 787 784 597 393 268
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2770 4623 1678
Size: 1092 1853 2531
Fragments arranged by size:
2531 1853 1092
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2220 2252 4117 4149 4191
Size: 32 135 32 648 32 1865 32 42
Cuts at: 4191 4223 1373
Size: 32 2626
Fragments arranged by size:
2626 1865 648 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3654
Fragments arranged by size:
3654 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2706 2892 3569 4717 4962 1606
Size: 1100 186 677 1148 245 2120
Fragments arranged by size:
2120 1148 1100 677 245 186
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4924
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2516 2834 4379 1132
Size: 97 1287 318 1545 2229
Fragments arranged by size:
2229 1545 1287 318 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 1108
Size: 1488 3988
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2495 1613
Size: 882 4594
PsiI TTA'TAA
Cuts at: 2277 2657 3046 2277
Size: 380 389 4707
Fragments arranged by size:
4707 389 380
PstI C_TGCA'G
Cuts at: 830 2828 830
Size: 1998 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2706 2706
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2824 3959 4640 4831 826
Size: 219 1779 1135 681 191 1471
Fragments arranged by size:
1779 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3580 4481 4743 820
Size: 197 926 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2424 2915 513
Size: 552 1359 491 3074
Fragments arranged by size:
3074 1359 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5436 5468 5436
Size: 32 5444
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI