(Circular) (Six-base) MAPSORT of: pFN21AB5971.seq Check: 5711 from: 1 to: 6259 pFN21AB5971 6259 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2789 2789 Size: 6259 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4069 278 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 3602 1937 Size: 1131 534 4594 Fragments arranged by size: 4594 1131 534 Acc65I G'GTAC_C Cuts at: 3580 3580 Size: 6259 AclI AA'CG_TT Cuts at: 1563 4386 4759 1563 Size: 2823 373 3063 Fragments arranged by size: 3063 2823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2203 2310 3203 3315 4322 5706 5974 1632 Size: 571 107 893 112 1007 1384 268 1917 Fragments arranged by size: 1917 1384 1007 893 571 268 112 107 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 2466 3483 5158 6135 1203 Size: 1263 1017 1675 977 1327 Fragments arranged by size: 1675 1327 1263 1017 977 AgeI A'CCGG_T Cuts at: 5090 5090 Size: 6259 AhdI GACnn_n'nnGTC Cuts at: 4988 4988 Size: 6259 AleI CACnn'nnGTG Cuts at: 1166 2974 1166 Size: 1808 4451 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2980 1184 Size: 1796 4463 AlwNI CAG_nnn'CTG Cuts at: 2082 2285 2798 4002 5574 2082 Size: 203 513 1204 1572 2767 Fragments arranged by size: 2767 1572 1204 513 203 ApaI G_GGCC'C Cuts at: 2404 2730 3393 2404 Size: 326 663 5270 Fragments arranged by size: 5270 663 326 ApaLI G'TGCA_C Cuts at: 2977 4316 5472 2977 Size: 1339 1156 3764 Fragments arranged by size: 3764 1339 1156 ApoI r'AATT_y Cuts at: 1493 3568 3794 3946 6055 1493 Size: 2075 226 152 2109 1697 Fragments arranged by size: 2109 2075 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 3093 3498 3584 1870 Size: 73 1150 405 86 4545 Fragments arranged by size: 4545 1150 405 86 73 BamHI G'GATC_C Cuts at: 3589 3956 3589 Size: 367 5892 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2703 2801 3005 3580 5035 Size: 325 217 627 916 98 204 575 1455 Cuts at: 5035 618 Size: 1842 Fragments arranged by size: 1842 1455 916 627 575 325 217 204 98 BanII G_rGCy'C Cuts at: 1391 2181 2383 2404 2730 2909 3393 3578 1391 Size: 790 202 21 326 179 484 185 4072 Fragments arranged by size: 4072 790 484 326 202 185 179 21 BbeI G_GCGC'C Cuts at: 2805 2805 Size: 6259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2453 3284 928 Size: 971 554 831 3903 Fragments arranged by size: 3903 971 831 554 BbvCI CC'TCA_GC Cuts at: 3141 3141 Size: 6259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2105 2139 4450 4484 1903 Size: 34 168 34 2311 34 3678 Fragments arranged by size: 3678 2311 168 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4151 5361 4151 Size: 1210 5049 BclI T'GATC_A Cuts at: 1548 2580 1548 Size: 1032 5227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 2304 2503 Size: 107 122 71 631 605 421 211 199 Cuts at: 2503 2561 4870 136 Size: 58 2309 1525 Fragments arranged by size: 2309 1525 631 605 421 211 199 122 107 71 58 BglII A'GATC_T Cuts at: 6254 6254 Size: 6259 BlpI GC'TnA_GC Cuts at: 3676 3676 Size: 6259 Bme1580I G_kGCm'C Cuts at: 1790 2404 2730 2969 2981 3393 4320 5476 1790 Size: 614 326 239 12 412 927 1156 2573 Fragments arranged by size: 2573 1156 927 614 412 326 239 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2273 2635 3141 Size: 416 53 83 399 24 856 362 506 Cuts at: 3141 3990 4948 442 Size: 849 958 1753 Fragments arranged by size: 1753 958 856 849 506 416 399 362 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 3267 3565 4919 1239 Size: 887 1141 298 1354 2579 Fragments arranged by size: 2579 1354 1141 887 298 Bpu10I CC'TnA_GC Cuts at: 2499 3141 2499 Size: 642 5617 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2956 2999 4384 5249 5547 2956 Size: 43 1385 865 298 3668 Fragments arranged by size: 3668 1385 865 298 43 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2038 4922 882 Size: 617 539 2884 2219 Fragments arranged by size: 2884 2219 617 539 BsaAI yAC'GTr Cuts at: 493 1204 4050 493 Size: 711 2846 2702 Fragments arranged by size: 2846 2702 711 BsaBI GATnn'nnATC Cuts at: 6253 6253 Size: 6259 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2802 4066 4448 5083 Size: 53 83 186 936 1269 1264 382 635 Cuts at: 5083 275 Size: 1451 Fragments arranged by size: 1451 1269 1264 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4692 5090 5364 5511 5922 4692 Size: 398 274 147 411 5029 Fragments arranged by size: 5029 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2807 2837 3506 3536 1178 Size: 30 165 30 1404 30 669 30 3901 Fragments arranged by size: 3901 1404 669 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 3511 1186 Size: 1210 1115 3934 Fragments arranged by size: 3934 1210 1115 BseYI C'CCAG_C Cuts at: 2075 2842 3009 3554 5462 5958 2075 Size: 767 167 545 1908 496 2376 Fragments arranged by size: 2376 1908 767 545 496 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2949 1163 Size: 573 1213 4473 Fragments arranged by size: 4473 1213 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3709 4470 5498 1540 Size: 275 186 1708 761 1028 2301 Fragments arranged by size: 2301 1708 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2981 4320 4405 5476 2981 Size: 1339 85 1071 3764 Fragments arranged by size: 3764 1339 1071 85 BsmI GAATG_Cn' Cuts at: 2651 3770 3863 2651 Size: 1119 93 5047 Fragments arranged by size: 5047 1119 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2383 2404 2730 2909 2969 2981 Size: 399 391 202 21 326 179 60 12 Cuts at: 2981 3393 3578 4320 4405 5476 1391 Size: 412 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 391 326 202 185 179 85 60 21 12 BspEI T'CCGG_A Cuts at: 5922 5922 Size: 6259 BspHI T'CATG_A Cuts at: 3267 4148 5878 3267 Size: 881 1730 3648 Fragments arranged by size: 3648 1730 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 3614 844 Size: 1945 825 3489 Fragments arranged by size: 3489 1945 825 BsrBI CCG'CTC Cuts at: 1394 1823 2503 4146 1394 Size: 429 680 1643 3507 Fragments arranged by size: 3507 1643 680 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4754 4928 65 Size: 1183 3506 174 1396 Fragments arranged by size: 3506 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4903 5090 1700 Size: 110 3093 187 2869 Fragments arranged by size: 3093 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6259 BssSI C'ACGA_G Cuts at: 4319 5331 4319 Size: 1012 5247 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6259 BstBI TT'CG_AA Cuts at: 5063 5063 Size: 6259 BstEII G'GTnAC_C Cuts at: 1184 2591 1184 Size: 1407 4852 BstYI r'GATC_y Cuts at: 1972 2421 3018 3589 3956 4341 4358 5810 5896 Size: 449 597 571 367 385 17 1452 86 Cuts at: 5896 5908 6254 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 597 571 449 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2895 2895 Size: 6259 BtgI C'CryG_G Cuts at: 513 1065 2091 2097 513 Size: 552 1026 6 4675 Fragments arranged by size: 4675 1026 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5520 Fragments arranged by size: 5520 683 56 BtsI GCAGTG_nn' Cuts at: 2914 3303 3775 4569 4589 2914 Size: 389 472 794 20 4584 Fragments arranged by size: 4584 794 472 389 20 ClaI AT'CG_AT Cuts at: 1554 2736 1554 Size: 1182 5077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6224 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5266 809 Size: 507 3950 1802 Fragments arranged by size: 3950 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 3300 3706 Size: 54 1425 50 275 18 264 1206 406 Cuts at: 3706 4595 6224 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1206 889 406 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3706 1537 Size: 275 1894 4090 Fragments arranged by size: 4090 1894 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2649 3179 3282 4189 1353 Size: 1296 530 103 907 3423 Fragments arranged by size: 3423 1296 907 530 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4846 5220 5366 387 Size: 1064 138 3257 374 146 1280 Fragments arranged by size: 3257 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2203 2310 3203 3267 3315 3565 Size: 393 494 77 107 893 64 48 250 Cuts at: 3565 4322 4919 5706 5974 1239 Size: 757 597 787 268 1524 Fragments arranged by size: 1524 893 787 757 597 494 393 268 250 107 77 64 48 EcoO109I rG'GnC_Cy Cuts at: 2400 2401 2659 2726 2727 2772 2853 3389 3390 Size: 1 258 67 1 45 81 536 1 Cuts at: 3390 3458 3495 3503 2400 Size: 68 37 8 5156 Fragments arranged by size: 5156 536 258 81 68 67 45 37 8 1 1 1 EcoRI G'AATT_C Cuts at: 3568 3946 3568 Size: 378 5881 EcoRV GAT'ATC Cuts at: 2131 2131 Size: 6259 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2539 3208 2539 Size: 669 5590 FspI TGC'GCA Cuts at: 3398 4765 3398 Size: 1367 4892 HaeII r_GCGC'y Cuts at: 1678 2805 3264 5406 1678 Size: 1127 459 2142 2531 Fragments arranged by size: 2531 2142 1127 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3506 3538 4900 4932 4974 Size: 32 135 32 1934 32 1362 32 42 Cuts at: 4974 5006 1373 Size: 32 2626 Fragments arranged by size: 2626 1934 1362 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2638 3069 3603 3849 669 Size: 988 281 700 431 534 246 3079 Fragments arranged by size: 3079 988 700 534 431 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6259 HpaI GTT'AAC Cuts at: 3849 3849 Size: 6259 KasI G'GCGC_C Cuts at: 2801 2801 Size: 6259 KpnI G_GTAC'C Cuts at: 3584 3584 Size: 6259 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2779 3039 5373 5557 1305 Size: 1474 260 2334 184 2007 Fragments arranged by size: 2334 2007 1474 260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3302 10 Size: 54 1425 343 1470 2967 Fragments arranged by size: 2967 1470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2628 2974 4217 4576 4735 518 Size: 648 1462 346 1243 359 159 2042 Fragments arranged by size: 2042 1462 1243 648 359 346 159 MspA1I CmG'CkG Cuts at: 1606 2748 3675 4352 5500 5745 1606 Size: 1142 927 677 1148 245 2120 Fragments arranged by size: 2120 1148 1142 927 677 245 NarI GG'CG_CC Cuts at: 2802 2802 Size: 6259 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5707 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 3108 3487 3617 5162 1132 Size: 97 1879 379 130 1545 2229 Fragments arranged by size: 2229 1879 1545 379 130 97 PasI CC'CwG_GG Cuts at: 1348 2489 2663 1348 Size: 1141 174 4944 Fragments arranged by size: 4944 1141 174 PciI A'CATG_T Cuts at: 3483 5158 3483 Size: 1675 4584 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6259 PfoI T'CCnGG_A Cuts at: 2142 2142 Size: 6259 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4328 4360 5841 5873 4328 Size: 32 1481 32 4714 Fragments arranged by size: 4714 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2659 2853 3458 3503 2659 Size: 194 605 45 5415 Fragments arranged by size: 5415 605 194 45 PshAI GACnn'nnGTC Cuts at: 1613 2858 1613 Size: 1245 5014 PsiI TTA'TAA Cuts at: 2202 3829 2202 Size: 1627 4632 PspOMI G'GGCC_C Cuts at: 2400 2726 3389 2400 Size: 326 663 5270 Fragments arranged by size: 5270 663 326 PstI C_TGCA'G Cuts at: 830 3078 3611 830 Size: 2248 533 3478 Fragments arranged by size: 3478 2248 533 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 SalI G'TCGA_C Cuts at: 1936 3067 3601 1936 Size: 1131 534 4594 Fragments arranged by size: 4594 1131 534 SapI GCTCTTCn'nnn_ Cuts at: 3179 3179 Size: 6259 SbfI CC_TGCA'GG Cuts at: 3611 3611 Size: 6259 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SfcI C'TryA_G Cuts at: 826 1045 3074 3607 4742 5423 5614 826 Size: 219 2029 533 1135 681 191 1471 Fragments arranged by size: 2029 1471 1135 681 533 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2304 2304 Size: 6259 SfoI GGC'GCC Cuts at: 2803 2803 Size: 6259 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 3095 3500 3586 1872 Size: 1223 405 86 4545 Fragments arranged by size: 4545 1223 405 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 2935 3014 4363 5264 5526 820 Size: 197 926 992 79 1349 901 262 1553 Fragments arranged by size: 1553 1349 992 926 901 262 197 79 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 3617 1132 Size: 2485 3774 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 2298 2497 2879 3139 1517 Size: 781 199 382 260 4637 Fragments arranged by size: 4637 781 382 260 199 StyI C'CwwG_G Cuts at: 513 1065 3698 513 Size: 552 2633 3074 Fragments arranged by size: 3074 2633 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2333 2579 2915 3023 4286 4303 4456 Size: 1255 753 246 336 108 1263 17 153 Cuts at: 4456 4641 5086 325 Size: 185 445 1498 Fragments arranged by size: 1498 1263 1255 753 445 336 246 185 153 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2469 4505 6217 Size: 274 80 33 51 1443 492 2036 1712 Cuts at: 6217 96 Size: 138 Fragments arranged by size: 2036 1712 1443 492 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6219 6251 6219 Size: 32 6227 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6259 XbaI T'CTAG_A Cuts at: 3595 3595 Size: 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2486 2670 2816 2011 Size: 475 184 146 5454 Fragments arranged by size: 5454 475 184 146 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6259 XmaI C'CCGG_G Cuts at: 1870 3093 3498 3584 1870 Size: 1223 405 86 4545 Fragments arranged by size: 4545 1223 405 86 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4067 276 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SrfI SwaI