Restriction map for pFN21AB5971
 (Linear) (Six-base) MAPSORT of: KIBB5971.seq  Check: 2195  from: 1  to: 1554



KIBB5971.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     784    1554 
   Size:        784     770
 
AccI GT'mk_AC

Cuts at:      0    1063    1554 
   Size:       1063     491
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     305    1198    1310    1554 
   Size:        198     107     893     112     244

  Fragments arranged by size: 

                 893     244     198     112     107
 
AflIII A'CryG_T

Cuts at:      0     461    1478    1554 
   Size:        461    1017      76

  Fragments arranged by size: 

                1017     461      76
 
AleI CACnn'nnGTG

Cuts at:      0     969    1554 
   Size:        969     585
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     975    1554 
   Size:        975     579
 
AlwNI CAG_nnn'CTG

Cuts at:      0      77     280     793    1554 
   Size:         77     203     513     761

  Fragments arranged by size: 

                 761     513     203      77
 
ApaI G_GGCC'C

Cuts at:      0     399     725    1388    1554 
   Size:        399     326     663     166

  Fragments arranged by size: 

                 663     399     326     166
 
ApaLI G'TGCA_C

Cuts at:      0     972    1554 
   Size:        972     582
 
AvaI C'yCGr_G

Cuts at:      0    1088    1493    1554 
   Size:       1088     405      61

  Fragments arranged by size: 

                1088     405      61
 
BanI G'GyrC_C

Cuts at:      0     698     796    1000    1554 
   Size:        698      98     204     554

  Fragments arranged by size: 

                 698     554     204      98
 
BanII G_rGCy'C

Cuts at:      0     176     378     399     725     904    1388    1554 
   Size:        176     202      21     326     179     484     166

  Fragments arranged by size: 

                 484     326     202     179     176     166      21
 
BbeI G_GCGC'C

Cuts at:      0     800    1554 
   Size:        800     754
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     448    1279    1554 
   Size:        448     831     275

  Fragments arranged by size: 

                 831     448     275
 
BbvCI CC'TCA_GC

Cuts at:      0    1136    1554 
   Size:       1136     418
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     100     134    1554 
   Size:        100      34    1420

  Fragments arranged by size: 

                1420     100      34
 
BclI T'GATC_A

Cuts at:      0     575    1554 
   Size:        575     979
 
BglI GCCn_nnn'nGGC

Cuts at:      0      88     299     498     556    1554 
   Size:         88     211     199      58     998

  Fragments arranged by size: 

                 998     211     199      88      58
 
Bme1580I G_kGCm'C

Cuts at:      0     399     725     964     976    1388    1554 
   Size:        399     326     239      12     412     166

  Fragments arranged by size: 

                 412     399     326     239     166      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     268     630    1136    1554 
   Size:        268     362     506     418

  Fragments arranged by size: 

                 506     418     362     268
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1262    1554 
   Size:        121    1141     292

  Fragments arranged by size: 

                1141     292     121
 
Bpu10I CC'TnA_GC

Cuts at:      0     494    1136    1554 
   Size:        494     642     418

  Fragments arranged by size: 

                 642     494     418
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     951     994    1554 
   Size:        951      43     560

  Fragments arranged by size: 

                 951     560      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      33    1554 
   Size:         33    1521
 
BsaHI Gr'CG_yC

Cuts at:      0     797    1554 
   Size:        797     757
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     802     832    1501    1531    1554 
   Size:        802      30     669      30      23

  Fragments arranged by size: 

                 802     669      30      30      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     391    1506    1554 
   Size:        391    1115      48

  Fragments arranged by size: 

                1115     391      48
 
BseYI C'CCAG_C

Cuts at:      0      70     837    1004    1549    1554 
   Size:         70     767     167     545       5

  Fragments arranged by size: 

                 767     545     167      70       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     944    1554 
   Size:        944     610
 
BsiHKAI G_wGCw'C

Cuts at:      0     976    1554 
   Size:        976     578
 
BsmI GAATG_Cn'

Cuts at:      0     646    1554 
   Size:        646     908
 
Bsp1286I G_dGCh'C

Cuts at:      0     176     378     399     725     904     964     976    1388 
   Size:        176     202      21     326     179      60      12     412

Cuts at:   1388    1554 
   Size:        166

  Fragments arranged by size: 

                 412     326     202     179     176     166      60      21
                  12
 
BspHI T'CATG_A

Cuts at:      0    1262    1554 
   Size:       1262     292
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     784    1554 
   Size:        784     770
 
BsrBI CCG'CTC

Cuts at:      0     498    1554 
   Size:        498    1056
 
BstEII G'GTnAC_C

Cuts at:      0     586    1554 
   Size:        586     968
 
BstYI r'GATC_y

Cuts at:      0     416    1013    1554 
   Size:        416     597     541

  Fragments arranged by size: 

                 597     541     416
 
Bsu36I CC'TnA_GG

Cuts at:      0     890    1554 
   Size:        890     664
 
BtgI C'CryG_G

Cuts at:      0      86      92    1554 
   Size:         86       6    1462

  Fragments arranged by size: 

                1462      86       6
 
BtsI GCAGTG_nn'

Cuts at:      0     909    1298    1554 
   Size:        909     389     256

  Fragments arranged by size: 

                 909     389     256
 
ClaI AT'CG_AT

Cuts at:      0     731    1554 
   Size:        731     823
 
EaeI y'GGCC_r

Cuts at:      0      89    1295    1554 
   Size:         89    1206     259

  Fragments arranged by size: 

                1206     259      89
 
EarI CTCTTCn'nnn_

Cuts at:      0     644    1174    1277    1554 
   Size:        644     530     103     277

  Fragments arranged by size: 

                 644     530     277     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     198     305    1198    1262    1310    1554 
   Size:        121      77     107     893      64      48     244

  Fragments arranged by size: 

                 893     244     121     107      77      64      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     395     396     654     721     722     767     848    1384 
   Size:        395       1     258      67       1      45      81     536

Cuts at:   1384    1385    1453    1490    1498    1554 
   Size:          1      68      37       8      56

  Fragments arranged by size: 

                 536     395     258      81      68      67      56      45
                  37       8       1       1       1
 
EcoRV GAT'ATC

Cuts at:      0     126    1554 
   Size:        126    1428
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     534    1203    1554 
   Size:        534     669     351

  Fragments arranged by size: 

                 669     534     351
 
FspI TGC'GCA

Cuts at:      0    1393    1554 
   Size:       1393     161
 
HaeII r_GCGC'y

Cuts at:      0     800    1259    1554 
   Size:        800     459     295

  Fragments arranged by size: 

                 800     459     295
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1501    1533    1554 
   Size:       1501      32      21

  Fragments arranged by size: 

                1501      32      21
 
HincII GTy'rAC

Cuts at:      0     633    1064    1554 
   Size:        633     431     490

  Fragments arranged by size: 

                 633     490     431
 
KasI G'GCGC_C

Cuts at:      0     796    1554 
   Size:        796     758
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     774    1034    1554 
   Size:        774     260     520

  Fragments arranged by size: 

                 774     520     260
 
MscI TGG'CCA

Cuts at:      0    1297    1554 
   Size:       1297     257
 
MslI CAynn'nnrTG

Cuts at:      0     623     969    1554 
   Size:        623     346     585

  Fragments arranged by size: 

                 623     585     346
 
MspA1I CmG'CkG

Cuts at:      0     743    1554 
   Size:        743     811
 
NarI GG'CG_CC

Cuts at:      0     797    1554 
   Size:        797     757
 
NspI r_CATG'y

Cuts at:      0    1103    1482    1554 
   Size:       1103     379      72

  Fragments arranged by size: 

                1103     379      72
 
PasI CC'CwG_GG

Cuts at:      0     484     658    1554 
   Size:        484     174     896

  Fragments arranged by size: 

                 896     484     174
 
PciI A'CATG_T

Cuts at:      0    1478    1554 
   Size:       1478      76
 
PfoI T'CCnGG_A

Cuts at:      0     137    1554 
   Size:        137    1417
 
PpuMI rG'GwC_Cy

Cuts at:      0     654     848    1453    1498    1554 
   Size:        654     194     605      45      56

  Fragments arranged by size: 

                 654     605     194      56      45
 
PshAI GACnn'nnGTC

Cuts at:      0     853    1554 
   Size:        853     701
 
PsiI TTA'TAA

Cuts at:      0     197    1554 
   Size:        197    1357
 
PspOMI G'GGCC_C

Cuts at:      0     395     721    1384    1554 
   Size:        395     326     663     170

  Fragments arranged by size: 

                 663     395     326     170
 
PstI C_TGCA'G

Cuts at:      0    1073    1554 
   Size:       1073     481
 
SalI G'TCGA_C

Cuts at:      0    1062    1554 
   Size:       1062     492
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1174    1554 
   Size:       1174     380
 
SfcI C'TryA_G

Cuts at:      0    1069    1554 
   Size:       1069     485
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     299    1554 
   Size:        299    1255
 
SfoI GGC'GCC

Cuts at:      0     798    1554 
   Size:        798     756
 
SmaI CCC'GGG

Cuts at:      0    1090    1495    1554 
   Size:       1090     405      59

  Fragments arranged by size: 

                1090     405      59
 
SmlI C'TyrA_G

Cuts at:      0     930    1009    1554 
   Size:        930      79     545

  Fragments arranged by size: 

                 930     545      79
 
StuI AGG'CCT

Cuts at:      0     293     492     874    1134    1554 
   Size:        293     199     382     260     420

  Fragments arranged by size: 

                 420     382     293     260     199
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     328     574     910    1018    1554 
   Size:        328     246     336     108     536

  Fragments arranged by size: 

                 536     336     328     246     108
 
TatI w'GTAC_w

Cuts at:      0     464    1554 
   Size:        464    1090
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     481     665     811    1554 
   Size:        481     184     146     743

  Fragments arranged by size: 

                 743     481     184     146
 
XmaI C'CCGG_G

Cuts at:      0    1088    1493    1554 
   Size:       1088     405      61

  Fragments arranged by size: 

                1088     405      61
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BclI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI   BstEII    BstYI   Bsu36I     BtgI     BtsI
     ClaI     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NspI     PasI     PciI     PfoI    PpuMI    PshAI     PsiI
   PspOMI     PstI     SalI     SapI     SfcI     SfiI     SfoI     SmaI
     SmlI     StuI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaBI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I    BtgZI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FseI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
    PflMI     PmeI     PmlI     PpiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII    SanDI     SbfI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com