Restriction map for
pFN21AA0413
(Circular) (Six-base) MAPSORT of: pFN21AA0413.seq Check: 576 from: 1 to: 8416
pFN21AA0413 8416 bp
With 182 enzymes: SgfI *
June 7, 2013 17:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4807 6226 278
Size: 53 83 186 936 3271 1419 2468
Fragments arranged by size:
3271 2468 1419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5759 1937
Size: 3822 4594
Acc65I G'GTAC_C
Cuts at: 5737 5737
Size: 8416
AclI AA'CG_TT
Cuts at: 1563 5580 6543 6916 1563
Size: 4017 963 373 3063
Fragments arranged by size:
4017 3063 963 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2990 3122 3216 3314 3675 4872 4874 6479
Size: 1358 132 94 98 361 1197 2 1605
Cuts at: 6479 7863 8131 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1605 1384 1358 1197 361 268 132
98 94 2
AflII C'TTAA_G
Cuts at: 820 1017 4876 5630 820
Size: 197 3859 754 3606
Fragments arranged by size:
3859 3606 754 197
AflIII A'CryG_T
Cuts at: 1203 5641 5643 7315 8292 1203
Size: 4438 2 1672 977 1327
Fragments arranged by size:
4438 1672 1327 977 2
AgeI A'CCGG_T
Cuts at: 7247 7247
Size: 8416
AhdI GACnn_n'nnGTC
Cuts at: 7145 7145
Size: 8416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2678 1184
Size: 1494 6922
AlwNI CAG_nnn'CTG
Cuts at: 2471 3821 4695 6159 7731 2471
Size: 1350 874 1464 1572 3156
Fragments arranged by size:
3156 1572 1464 1350 874
ApaLI G'TGCA_C
Cuts at: 2955 6473 7629 2955
Size: 3518 1156 3742
Fragments arranged by size:
3742 3518 1156
ApoI r'AATT_y
Cuts at: 1493 2850 3545 3598 4024 4201 4336 5049 5134
Size: 1357 695 53 426 177 135 713 85
Cuts at: 5134 5725 5951 6103 8212 1493
Size: 591 226 152 2109 1697
Fragments arranged by size:
2109 1697 1357 713 695 591 426 226
177 152 135 85 53
AseI AT'TA_AT
Cuts at: 160 1032 4314 4818 6970 160
Size: 872 3282 504 2152 1606
Fragments arranged by size:
3282 2152 1606 872 504
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8416
AvaI C'yCGr_G
Cuts at: 1870 1943 5741 1870
Size: 73 3798 4545
Fragments arranged by size:
4545 3798 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5065 5098 5065
Size: 33 8383
BamHI G'GATC_C
Cuts at: 5746 6113 5746
Size: 367 8049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 5737 7192 618
Size: 325 217 627 1367 2583 1455 1842
Fragments arranged by size:
2583 1842 1455 1367 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 5735 1391
Size: 754 3590 4072
Fragments arranged by size:
4072 3590 754
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3108 3213 3411 5024 928
Size: 971 1209 105 198 1613 4320
Fragments arranged by size:
4320 1613 1209 971 198 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6607 6641 1903
Size: 34 4670 34 3678
Fragments arranged by size:
4670 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6308 7518 6308
Size: 1210 7206
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5352 7027 136
Size: 107 122 71 631 605 3680 1675 1525
Fragments arranged by size:
3680 1675 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2248 3124 5301 8411 2248
Size: 876 2177 3110 2253
Fragments arranged by size:
3110 2253 2177 876
BlpI GC'TnA_GC
Cuts at: 3711 5833 3711
Size: 2122 6294
Bme1580I G_kGCm'C
Cuts at: 1790 2959 6477 7633 1790
Size: 1169 3518 1156 2573
Fragments arranged by size:
3518 2573 1169 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3677 4044 4134
Size: 416 53 83 399 24 2260 367 90
Cuts at: 4134 4546 5598 6147 7105 442
Size: 412 1052 549 958 1753
Fragments arranged by size:
2260 1753 1052 958 549 416 412 399
367 90 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5326 5514 5577 7076 1239
Size: 4087 188 63 1499 2579
Fragments arranged by size:
4087 2579 1499 188 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4335 4842 6541 7406 7704 4335
Size: 507 1699 865 298 5047
Fragments arranged by size:
5047 1699 865 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2688 3677 7079 882
Size: 617 1189 989 3402 2219
Fragments arranged by size:
3402 2219 1189 989 617
BsaAI yAC'GTr
Cuts at: 493 1204 2209 2966 5644 6207 493
Size: 711 1005 757 2678 563 2702
Fragments arranged by size:
2702 2678 1005 757 711 563
BsaBI GATnn'nnATC
Cuts at: 4161 8410 4161
Size: 4249 4167
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4804 6223 6605 7240
Size: 53 83 186 936 3271 1419 382 635
Cuts at: 7240 275
Size: 1451
Fragments arranged by size:
3271 1451 1419 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6849 7247 7521 7668 8079 6849
Size: 398 274 147 411 7186
Fragments arranged by size:
7186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5493 5523 1178
Size: 30 165 30 4090 30 4071
Fragments arranged by size:
4090 4071 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8416
BseYI C'CCAG_C
Cuts at: 2982 5505 7619 8115 2982
Size: 2523 2114 496 3283
Fragments arranged by size:
3283 2523 2114 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3606 1163
Size: 573 1870 5973
Fragments arranged by size:
5973 1870 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5866 6627 7655 1540
Size: 275 186 3865 761 1028 2301
Fragments arranged by size:
3865 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2959 4065 5470 6477 6562 7633 2959
Size: 1106 1405 1007 85 1071 3742
Fragments arranged by size:
3742 1405 1106 1071 1007 85
BsmI GAATG_Cn'
Cuts at: 2274 2377 2655 3750 3962 5927 6020 2274
Size: 103 278 1095 212 1965 93 4670
Fragments arranged by size:
4670 1965 1095 278 212 103 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2654 2654
Size: 8416
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2959 4065 5470 5735 6477 6562
Size: 399 355 814 1106 1405 265 742 85
Cuts at: 6562 7633 1391
Size: 1071 2174
Fragments arranged by size:
2174 1405 1106 1071 814 742 399 355
265 85
BspEI T'CCGG_A
Cuts at: 8079 8079
Size: 8416
BspHI T'CATG_A
Cuts at: 3541 3937 5332 6305 8035 3541
Size: 396 1395 973 1730 3922
Fragments arranged by size:
3922 1730 1395 973 396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4001 5771 844
Size: 3157 1770 3489
Fragments arranged by size:
3489 3157 1770
BsrBI CCG'CTC
Cuts at: 1394 1823 6303 1394
Size: 429 4480 3507
Fragments arranged by size:
4480 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4896 6911 7085 65
Size: 1183 3648 2015 174 1396
Fragments arranged by size:
3648 2015 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7060 7247 1700
Size: 110 5250 187 2869
Fragments arranged by size:
5250 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3661 96
Size: 3565 4851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8416
BssSI C'ACGA_G
Cuts at: 6476 7488 6476
Size: 1012 7404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4541 794
Size: 3747 4669
BstBI TT'CG_AA
Cuts at: 7220 7220
Size: 8416
BstEII G'GTnAC_C
Cuts at: 1184 2419 1184
Size: 1235 7181
BstYI r'GATC_y
Cuts at: 1972 2248 3124 3334 5301 5746 6113 6498 6515
Size: 276 876 210 1967 445 367 385 17
Cuts at: 6515 7967 8053 8065 8411 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1967 1452 876 445 385 367 346
276 210 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2827 2827
Size: 8416
BtgI C'CryG_G
Cuts at: 513 1065 2004 5497 513
Size: 552 939 3493 3432
Fragments arranged by size:
3493 3432 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7677
Fragments arranged by size:
7677 683 56
BtsI GCAGTG_nn'
Cuts at: 4213 4542 5932 6726 6746 4213
Size: 329 1390 794 20 5883
Fragments arranged by size:
5883 1390 794 329 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8381
DraI TTT'AAA
Cuts at: 3621 5720 3621
Size: 2099 6317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7423 809
Size: 507 6107 1802
Fragments arranged by size:
6107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3444 5863 6752
Size: 54 1425 50 275 18 1614 2419 889
Cuts at: 6752 8381 8
Size: 1629 43
Fragments arranged by size:
2419 1629 1614 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5863 1537
Size: 275 4051 4090
Fragments arranged by size:
4090 4051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2293 2628 6346 1353
Size: 762 178 335 3718 3423
Fragments arranged by size:
3718 3423 762 335 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3794 7003 7377 7523 387
Size: 1064 138 2205 3209 374 146 1280
Fragments arranged by size:
3209 2205 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2990 3122 3216 3314 3675 4872 4874
Size: 393 1358 132 94 98 361 1197 2
Cuts at: 4874 5326 5514 5577 6479 7076 7863 8131 1239
Size: 452 188 63 902 597 787 268 1524
Fragments arranged by size:
1524 1358 1197 902 787 597 452 393
361 268 188 132 98 94 63 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3140 3140
Size: 8416
EcoO109I rG'GnC_Cy
Cuts at: 2795 3524 4345 2795
Size: 729 821 6866
Fragments arranged by size:
6866 821 729
EcoRI G'AATT_C
Cuts at: 4024 5725 6103 4024
Size: 1701 378 6337
Fragments arranged by size:
6337 1701 378
EcoRV GAT'ATC
Cuts at: 3371 3371
Size: 8416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3054 3054
Size: 8416
FspI TGC'GCA
Cuts at: 6922 6922
Size: 8416
HaeII r_GCGC'y
Cuts at: 1678 7563 1678
Size: 5885 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3090 3122 3340 3372 3599
Size: 32 135 32 1518 32 218 32 227
Cuts at: 3599 3631 4049 4081 4274 4306 4627 4640 4659
Size: 32 418 32 193 32 321 13 19
Cuts at: 4659 4672 4828 4840 4860 4872 5299 5331 7057
Size: 13 156 12 20 12 427 32 1726
Cuts at: 7057 7089 7131 7163 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1726 1518 427 418 321 227 218
193 156 135 42 32 32 32 32
32 32 32 32 32 32 20 19
13 13 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4792 5760 6006 669
Size: 988 281 2854 968 246 3079
Fragments arranged by size:
3079 2854 988 968 281 246
HindIII A'AGCT_T
Cuts at: 748 2727 4238 4274 748
Size: 1979 1511 36 4890
Fragments arranged by size:
4890 1979 1511 36
HpaI GTT'AAC
Cuts at: 6006 6006
Size: 8416
KpnI G_GTAC'C
Cuts at: 5741 5741
Size: 8416
MfeI C'AATT_G
Cuts at: 4078 5069 6015 4078
Size: 991 946 6479
Fragments arranged by size:
6479 991 946
MluI A'CGCG_T
Cuts at: 8292 8292
Size: 8416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2568 7530 7714 1305
Size: 1263 4962 184 2007
Fragments arranged by size:
4962 2007 1263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3446 10
Size: 54 1425 343 1614 4980
Fragments arranged by size:
4980 1614 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 3816 3942 4053 4316 4455 5463
Size: 648 1494 1156 126 111 263 139 1008
Cuts at: 5463 6374 6733 6892 518
Size: 911 359 159 2042
Fragments arranged by size:
2042 1494 1156 1008 911 648 359 263
159 139 126 111
MspA1I CmG'CkG
Cuts at: 1606 4259 5832 6509 7657 7902 1606
Size: 2653 1573 677 1148 245 2120
Fragments arranged by size:
2653 2120 1573 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 5497 513
Size: 552 939 3493 3432
Fragments arranged by size:
3493 3432 939 552
NdeI CA'TA_TG
Cuts at: 387 5665 387
Size: 5278 3138
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8416
NotI GC'GGCC_GC
Cuts at: 5863 5863
Size: 8416
NspI r_CATG'y
Cuts at: 1132 1229 5774 7319 1132
Size: 97 4545 1545 2229
Fragments arranged by size:
4545 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2507 1348
Size: 1159 7257
PciI A'CATG_T
Cuts at: 7315 7315
Size: 8416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2982 1108
Size: 1874 6542
PmeI GTTT'AAAC
Cuts at: 5720 5720
Size: 8416
PmlI CAC'GTG
Cuts at: 5644 5644
Size: 8416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6485 6517 7998 8030 6485
Size: 32 1481 32 6871
Fragments arranged by size:
6871 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2795 4345 2795
Size: 1550 6866
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8416
PsiI TTA'TAA
Cuts at: 2912 5986 2912
Size: 3074 5342
PstI C_TGCA'G
Cuts at: 830 2467 2571 3219 3720 4220 5768 830
Size: 1637 104 648 501 500 1548 3478
Fragments arranged by size:
3478 1637 1548 648 501 500 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8416
PvuII CAG'CTG
Cuts at: 4259 4259
Size: 8416
SalI G'TCGA_C
Cuts at: 1936 5758 1936
Size: 3822 4594
SbfI CC_TGCA'GG
Cuts at: 5768 5768
Size: 8416
ScaI AGT'ACT
Cuts at: 2990 4505 4556 5235 6664 8376 2990
Size: 1515 51 679 1429 1712 3030
Fragments arranged by size:
3030 1712 1515 1429 679 51
SfcI C'TryA_G
Cuts at: 826 1045 2463 2567 3215 3716 3969 4216 5764
Size: 219 1418 104 648 501 253 247 1548
Cuts at: 5764 6899 7580 7771 826
Size: 1135 681 191 1471
Fragments arranged by size:
1548 1471 1418 1135 681 648 501 253
247 219 191 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8416
SmaI CCC'GGG
Cuts at: 1872 5743 1872
Size: 3871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4350 4857 4876 5630 6520 7421
Size: 197 926 2407 507 19 754 890 901
Cuts at: 7421 7683 820
Size: 262 1553
Fragments arranged by size:
2407 1553 926 901 890 754 507 262
197 19
SnaBI TAC'GTA
Cuts at: 493 2209 6207 493
Size: 1716 3998 2702
Fragments arranged by size:
3998 2702 1716
SpeI A'CTAG_T
Cuts at: 152 4042 4454 152
Size: 3890 412 4114
Fragments arranged by size:
4114 3890 412
SphI G_CATG'C
Cuts at: 1132 5774 1132
Size: 4642 3774
SspI AAT'ATT
Cuts at: 5 52 2629 3658 5185 6340 5
Size: 47 2577 1029 1527 1155 2081
Fragments arranged by size:
2577 2081 1527 1155 1029 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2678 5497 5855 513
Size: 552 939 674 2819 358 3074
Fragments arranged by size:
3074 2819 939 674 552 358
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2691 2707 5497 6443 6460 6613 6798
Size: 1255 1111 16 2790 946 17 153 185
Cuts at: 6798 7243 325
Size: 445 1498
Fragments arranged by size:
2790 1498 1255 1111 946 445 185 153
17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2988 3661 4045
Size: 274 80 33 51 1443 1011 673 384
Cuts at: 4045 4503 4554 4893 5233 6662 8374 96
Size: 458 51 339 340 1429 1712 138
Fragments arranged by size:
1712 1443 1429 1011 673 458 384 340
339 274 138 80 51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 8376 8408 5493
Size: 32 2851 32 5501
Fragments arranged by size:
5501 2851 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5213 1316
Size: 3897 4519
XbaI T'CTAG_A
Cuts at: 3061 3922 5752 3061
Size: 861 1830 5725
Fragments arranged by size:
5725 1830 861
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 2431
Size: 8416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8416
XmaI C'CCGG_G
Cuts at: 1870 5741 1870
Size: 3871 4545
XmnI GAAnn'nnTTC
Cuts at: 3862 4325 6545 3862
Size: 463 2220 5733
Fragments arranged by size:
5733 2220 463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4805 6224 276
Size: 53 83 186 936 3271 1419 2468
Fragments arranged by size:
3271 2468 1419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI