Restriction map for
pFN21AA0413
(Linear) (Six-base) MAPSORT of: KIAA0413.seq Check: 8073 from: 1 to: 3711
KIAA0413.orf
With 182 enzymes: SgfI *
July 15, 2010 18:28 ..
AatII G_ACGT'C
Cuts at: 0 2802 3711
Size: 2802 909
AclI AA'CG_TT
Cuts at: 0 3575 3711
Size: 3575 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1117 1211 1309 1670 2867 2869 3711
Size: 985 132 94 98 361 1197 2 842
Fragments arranged by size:
1197 985 842 361 132 98 94 2
AflII C'TTAA_G
Cuts at: 0 2871 3625 3711
Size: 2871 754 86
Fragments arranged by size:
2871 754 86
AflIII A'CryG_T
Cuts at: 0 3636 3638 3711
Size: 3636 2 73
Fragments arranged by size:
3636 73 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 673 3711
Size: 673 3038
AlwNI CAG_nnn'CTG
Cuts at: 0 466 1816 2690 3711
Size: 466 1350 874 1021
Fragments arranged by size:
1350 1021 874 466
ApaLI G'TGCA_C
Cuts at: 0 950 3711
Size: 950 2761
ApoI r'AATT_y
Cuts at: 0 845 1540 1593 2019 2196 2331 3044 3129
Size: 845 695 53 426 177 135 713 85
Cuts at: 3129 3711
Size: 582
Fragments arranged by size:
845 713 695 582 426 177 135 85
53
AseI AT'TA_AT
Cuts at: 0 2309 2813 3711
Size: 2309 504 898
Fragments arranged by size:
2309 898 504
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3060 3093 3711
Size: 3060 33 618
Fragments arranged by size:
3060 618 33
BanI G'GyrC_C
Cuts at: 0 1149 3711
Size: 1149 2562
BanII G_rGCy'C
Cuts at: 0 140 3711
Size: 140 3571
BbsI GAAGACnn'nnnn_
Cuts at: 0 1103 1208 1406 3019 3711
Size: 1103 105 198 1613 692
Fragments arranged by size:
1613 1103 692 198 105
BglI GCCn_nnn'nGGC
Cuts at: 0 3347 3711
Size: 3347 364
BglII A'GATC_T
Cuts at: 0 243 1119 3296 3711
Size: 243 876 2177 415
Fragments arranged by size:
2177 876 415 243
BlpI GC'TnA_GC
Cuts at: 0 1706 3711
Size: 1706 2005
Bme1580I G_kGCm'C
Cuts at: 0 954 3711
Size: 954 2757
BmrI ACTGGGnnnn_n'
Cuts at: 0 1672 2039 2129 2541 3593 3711
Size: 1672 367 90 412 1052 118
Fragments arranged by size:
1672 1052 412 367 118 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3321 3509 3572 3711
Size: 3321 188 63 139
Fragments arranged by size:
3321 188 139 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2330 2837 3711
Size: 2330 507 874
Fragments arranged by size:
2330 874 507
BsaI GGTCTCn'nnnn_
Cuts at: 0 683 1672 3711
Size: 683 989 2039
Fragments arranged by size:
2039 989 683
BsaAI yAC'GTr
Cuts at: 0 204 961 3639 3711
Size: 204 757 2678 72
Fragments arranged by size:
2678 757 204 72
BsaBI GATnn'nnATC
Cuts at: 0 2156 3711
Size: 2156 1555
BsaHI Gr'CG_yC
Cuts at: 0 2799 3711
Size: 2799 912
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3488 3518 3711
Size: 3488 30 193
Fragments arranged by size:
3488 193 30
BseYI C'CCAG_C
Cuts at: 0 977 3500 3711
Size: 977 2523 211
Fragments arranged by size:
2523 977 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1601 3711
Size: 1601 2110
BsiHKAI G_wGCw'C
Cuts at: 0 954 2060 3465 3711
Size: 954 1106 1405 246
Fragments arranged by size:
1405 1106 954 246
BsmI GAATG_Cn'
Cuts at: 0 269 372 650 1745 1957 3711
Size: 269 103 278 1095 212 1754
Fragments arranged by size:
1754 1095 278 269 212 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 649 3711
Size: 649 3062
Bsp1286I G_dGCh'C
Cuts at: 0 140 954 2060 3465 3711
Size: 140 814 1106 1405 246
Fragments arranged by size:
1405 1106 814 246 140
BspHI T'CATG_A
Cuts at: 0 1536 1932 3327 3711
Size: 1536 396 1395 384
Fragments arranged by size:
1536 1395 396 384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1996 3711
Size: 1996 1715
BsrDI GCAATG_nn'
Cuts at: 0 2891 3711
Size: 2891 820
BsrGI T'GTAC_A
Cuts at: 0 1656 3711
Size: 1656 2055
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2536 3711
Size: 2536 1175
BstEII G'GTnAC_C
Cuts at: 0 414 3711
Size: 414 3297
BstYI r'GATC_y
Cuts at: 0 243 1119 1329 3296 3711
Size: 243 876 210 1967 415
Fragments arranged by size:
1967 876 415 243 210
Bsu36I CC'TnA_GG
Cuts at: 0 822 3711
Size: 822 2889
BtgI C'CryG_G
Cuts at: 0 3492 3711
Size: 3492 219
BtsI GCAGTG_nn'
Cuts at: 0 2208 2537 3711
Size: 2208 329 1174
Fragments arranged by size:
2208 1174 329
DraI TTT'AAA
Cuts at: 0 1616 3711
Size: 1616 2095
EaeI y'GGCC_r
Cuts at: 0 1439 3711
Size: 1439 2272
EarI CTCTTCn'nnn_
Cuts at: 0 110 288 623 3711
Size: 110 178 335 3088
Fragments arranged by size:
3088 335 178 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1789 3711
Size: 1789 1922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1117 1211 1309 1670 2867 2869 3321
Size: 985 132 94 98 361 1197 2 452
Cuts at: 3321 3509 3572 3711
Size: 188 63 139
Fragments arranged by size:
1197 985 452 361 188 139 132 98
94 63 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1135 3711
Size: 1135 2576
EcoO109I rG'GnC_Cy
Cuts at: 0 790 1519 2340 3711
Size: 790 729 821 1371
Fragments arranged by size:
1371 821 790 729
EcoRI G'AATT_C
Cuts at: 0 2019 3711
Size: 2019 1692
EcoRV GAT'ATC
Cuts at: 0 1366 3711
Size: 1366 2345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1049 3711
Size: 1049 2662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1085 1117 1335 1367 1594 1626 2044 2076
Size: 1085 32 218 32 227 32 418 32
Cuts at: 2076 2269 2301 2622 2635 2654 2667 2823 2835
Size: 193 32 321 13 19 13 156 12
Cuts at: 2835 2855 2867 3294 3326 3711
Size: 20 12 427 32 385
Fragments arranged by size:
1085 427 418 385 321 227 218 193
156 32 32 32 32 32 32 20
19 13 13 12 12
HincII GTy'rAC
Cuts at: 0 2787 3711
Size: 2787 924
HindIII A'AGCT_T
Cuts at: 0 722 2233 2269 3711
Size: 722 1511 36 1442
Fragments arranged by size:
1511 1442 722 36
MfeI C'AATT_G
Cuts at: 0 2073 3064 3711
Size: 2073 991 647
Fragments arranged by size:
2073 991 647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 3711
Size: 563 3148
MscI TGG'CCA
Cuts at: 0 1441 3711
Size: 1441 2270
MslI CAynn'nnrTG
Cuts at: 0 655 1811 1937 2048 2311 2450 3458 3711
Size: 655 1156 126 111 263 139 1008 253
Fragments arranged by size:
1156 1008 655 263 253 139 126 111
MspA1I CmG'CkG
Cuts at: 0 2254 3711
Size: 2254 1457
NcoI C'CATG_G
Cuts at: 0 3492 3711
Size: 3492 219
NdeI CA'TA_TG
Cuts at: 0 3660 3711
Size: 3660 51
PasI CC'CwG_GG
Cuts at: 0 502 3711
Size: 502 3209
PflMI CCAn_nnn'nTGG
Cuts at: 0 977 3711
Size: 977 2734
PmlI CAC'GTG
Cuts at: 0 3639 3711
Size: 3639 72
PpuMI rG'GwC_Cy
Cuts at: 0 790 2340 3711
Size: 790 1550 1371
Fragments arranged by size:
1550 1371 790
PsiI TTA'TAA
Cuts at: 0 907 3711
Size: 907 2804
PstI C_TGCA'G
Cuts at: 0 462 566 1214 1715 2215 3711
Size: 462 104 648 501 500 1496
Fragments arranged by size:
1496 648 501 500 462 104
PvuII CAG'CTG
Cuts at: 0 2254 3711
Size: 2254 1457
ScaI AGT'ACT
Cuts at: 0 985 2500 2551 3230 3711
Size: 985 1515 51 679 481
Fragments arranged by size:
1515 985 679 481 51
SfcI C'TryA_G
Cuts at: 0 458 562 1210 1711 1964 2211 3711
Size: 458 104 648 501 253 247 1500
Fragments arranged by size:
1500 648 501 458 253 247 104
SmlI C'TyrA_G
Cuts at: 0 2345 2852 2871 3625 3711
Size: 2345 507 19 754 86
Fragments arranged by size:
2345 754 507 86 19
SnaBI TAC'GTA
Cuts at: 0 204 3711
Size: 204 3507
SpeI A'CTAG_T
Cuts at: 0 2037 2449 3711
Size: 2037 412 1262
Fragments arranged by size:
2037 1262 412
SspI AAT'ATT
Cuts at: 0 624 1653 3180 3711
Size: 624 1029 1527 531
Fragments arranged by size:
1527 1029 624 531
StyI C'CwwG_G
Cuts at: 0 673 3492 3711
Size: 673 2819 219
Fragments arranged by size:
2819 673 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 686 702 3492 3711
Size: 686 16 2790 219
Fragments arranged by size:
2790 686 219 16
TatI w'GTAC_w
Cuts at: 0 983 1656 2040 2498 2549 2888 3228 3711
Size: 983 673 384 458 51 339 340 483
Fragments arranged by size:
983 673 483 458 384 340 339 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3488 3520 3711
Size: 3488 32 191
Fragments arranged by size:
3488 191 32
Tth111I GACn'n_nGTC
Cuts at: 0 3208 3711
Size: 3208 503
XbaI T'CTAG_A
Cuts at: 0 1056 1917 3711
Size: 1056 861 1794
Fragments arranged by size:
1794 1056 861
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 426 3711
Size: 426 3285
XmnI GAAnn'nnTTC
Cuts at: 0 1857 2320 3711
Size: 1857 463 1391
Fragments arranged by size:
1857 1391 463
ZraI GAC'GTC
Cuts at: 0 2800 3711
Size: 2800 911
Enzymes that do cut:
AatII AclI AcuI AflII AflIII AlfI AlwNI ApaLI
ApoI AseI BaeI BanI BanII BbsI BglI BglII
BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrGI BstAPI BstEII BstYI Bsu36I
BtgI BtsI DraI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NcoI NdeI PasI PflMI
PmlI PpuMI PsiI PstI PvuII ScaI SfcI SmlI
SnaBI SpeI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AgeI AhdI AleI AloI
ApaI AscI AsiSI AvaI AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BmgBI BmtI BplI Bpu10I
BsaWI BseRI BsiEI BsiWI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraIII
DrdI EagI EcoICRI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SphI SrfI StuI SwaI XhoI XmaI