Restriction map for pFN21AE0213
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# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstEII      
BstPI       BstXI       Bsu36I      Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        FspI        HaeIII      HapII       HgaI        
Hin1I       HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        Sau96I      SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          737  GTATAC               GT^[AC][GT]AC
AccII         679  CGCG                 CG^CG
AlwI          189  GGATC                GGATC(4/5)
AseI          237  ATTAAT               AT^TAAT
AsnI          237  ATTAAT               AT^TAAT
Asp700I        64  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI          795  TGATCA               T^GATCA
BlpI          142  GCTCAGC              GC^T[ACGT]AGC
BmyI           93  GGGCAC               G[AGT]GC[ACT]^C
BpmI          545  CTGGAG               CTGGAG(16/14)
Bpu1102I      142  GCTCAGC              GC^T[ACGT]AGC
BsaJI          36  CCCTGG               C^C[ACGT][ACGT]GG
BseRI         103  GAGGAG               GAGGAG(10/8)
BsiYI         487  CCTGATGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          487  CCTGATGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I       93  GGGCAC               G[AGT]GC[ACT]^C
BspMI         741  ACCTGC               ACCTGC(4/8)
BsrBI          49  GAGCGG               CCGCTC(-3/-3)
BsrI          220  ACTGG                ACTGG(1/-1)
Bst1107I      737  GTATAC               GTA^TAC
BstBI         731  TTCGAA               TT^CGAA
BstUI         679  CGCG                 CG^CG
BstYI         189  GGATCT               [GA]^GATC[CT]
BstZ17I       737  GTATAC               GTA^TAC
Cac8I          51  GCGGGC               GC[ACGT]^[ACGT]GC
CelII         142  GCTCAGC              GC^T[ACGT]AGC
CfoI          147  GCGC                 GCG^C
DraIII        599  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
FauI           51  GCGGG                CCCGC(4/6)
FbaI          795  TGATCA               T^GATCA
HaeII         146  AGCGCC               [GA]GCGC^[CT]
HhaI          147  GCGC                 GCG^C
HinP1I        147  GCGC                 G^CGC
HincII        123  GTTGAC               GT[CT]^[GA]AC
HindII        123  GTTGAC               GT[CT]^[GA]AC
HphI          584  TCACC                GGTGA(8/7)
MaeII         419  ACGT                 A^CGT
MaeIII         25  GTGAC                ^GT[ACGT]AC
MflI          189  GGATCT               [GA]^GATC[CT]
MspA1I        448  CAGCGG               C[AC]G^C[GT]G
MvnI          679  CGCG                 CG^CG
MwoI          745  GCCTACCTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          291  ACATGC               [GA]CATG^[CT]
NspV          731  TTCGAA               TT^CGAA
PshBI         237  ATTAAT               AT^TAAT
PstI          161  CTGCAG               CTGCA^G
SapI          931  GCTCTTC              GCTCTTC(1/4)
ScaI          324  AGTACT               AGT^ACT
SfcI          161  CTGCAG               C^T[GA][CT]AG
SfuI          731  TTCGAA               TT^CGAA
TaqI          732  TCGA                 T^CGA
Tsp45I         25  GTGAC                ^GT[GC]AC
TspRI          21  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       732  TCGA                 T^CGA
XhoII         189  GGATCT               [GA]^GATC[CT]
XmnI           64  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AcsI          226  AAATTT               [GA]^AATT[CT]
              870  GAATTT              
ApoI          226  AAATTT               [GA]^AATT[CT]
              870  GAATTT              
BbvI          163  GCAGC                GCAGC(8/12)
              939  GCTGC               
BsaI          482  GAGACC               GGTCTC(1/5)
              757  GAGACC              
EarI           68  CTCTTC               CTCTTC(1/4)
              932  CTCTTC              
Fnu4HI        163  GCAGC                GC^[ACGT]GC
              939  GCTGC               
ItaI          163  GCAGC                GC^[ACGT]GC
              939  GCTGC               
Ksp632I        68  CTCTTC               CTCTTC(1/4)
              932  CTCTTC              
SmlI          402  CTCAAG               C^T[CT][GA]AG
              509  CTCAAG              
TseI          163  GCAGC                G^C[AT]GC
              939  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AfaI        AluI        BsmAI       BsmFI       
BssKI       BstF5I      BstNI       DdeI        DpnII       
EcoRII      FokI        HinfI       MboI        MboII       
MnlI        MseI        MvaI        NdeII       NlaIII      
PleI        RsaI        Sau3AI      ScrFI       SfaNI       
Tru9I       Tsp509I     
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