Restriction map for pFN21AE0213
 (Circular) (Six-base) MAPSORT of: pFN21AE0213.seq  Check: 3872  from: 1  to: 5665



pFN21AE0213  5665 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:45  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3475     278 
   Size:         53      83     186     936    1939    2468

  Fragments arranged by size: 

                2468    1939     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2743    3008    1937 
   Size:        806     265    4594

  Fragments arranged by size: 

                4594     806     265
 
Acc65I G'GTAC_C

Cuts at:   2986    2986 
   Size:       5665
 
AclI AA'CG_TT

Cuts at:   1563    3792    4165    1563 
   Size:       2229     373    3063

  Fragments arranged by size: 

                3063    2229     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2382    2424    2459    3728    5112    5380    1632 
   Size:        750      42      35    1269    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1269     750     268      42      35
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5468
 
AflIII A'CryG_T

Cuts at:   1203    4564    5541    1203 
   Size:       3361     977    1327

  Fragments arranged by size: 

                3361    1327     977
 
AgeI A'CCGG_T

Cuts at:   4496    4496 
   Size:       5665
 
AhdI GACnn_n'nnGTC

Cuts at:   4394    4394 
   Size:       5665
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5665
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5665
 
AlwNI CAG_nnn'CTG

Cuts at:   3408    4980    3408 
   Size:       1572    4093
 
ApaLI G'TGCA_C

Cuts at:   3722    4878    3722 
   Size:       1156    4509
 
ApoI r'AATT_y

Cuts at:   1493    2231    2875    2974    3200    3352    5461    1493 
   Size:        738     644      99     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     738     644     226     152      99
 
AseI AT'TA_AT

Cuts at:    160    1032    2243    4219     160 
   Size:        872    1211    1976    1606

  Fragments arranged by size: 

                1976    1606    1211     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5665
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2990    1870 
   Size:         73    1047    4545

  Fragments arranged by size: 

                4545    1047      73
 
BamHI G'GATC_C

Cuts at:   2995    3362    2995 
   Size:        367    5298
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2986    4441     618 
   Size:        325     217     627    1199    1455    1842

  Fragments arranged by size: 

                1842    1455    1199     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2984    1391 
   Size:       1593    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4694
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3856    3890    1903 
   Size:         34    1919      34    3678

  Fragments arranged by size: 

                3678    1919      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3557    4767    3557 
   Size:       1210    4455
 
BclI T'GATC_A

Cuts at:   1548    2800    1548 
   Size:       1252    4413
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4276     136 
   Size:        107     122      71     631     605    2604    1525

  Fragments arranged by size: 

                2604    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5660    5660 
   Size:       5665
 
BlpI GC'TnA_GC

Cuts at:   2148    3082    2148 
   Size:        934    4731
 
Bme1580I G_kGCm'C

Cuts at:   1790    2102    3726    4882    1790 
   Size:        312    1624    1156    2573

  Fragments arranged by size: 

                2573    1624    1156     312
 
BmgBI CAC'GTC

Cuts at:   1318    2425    1318 
   Size:       1107    4558
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3396    4354     442 
   Size:        416      53      83     399      24    1979     958    1753

  Fragments arranged by size: 

                1979    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5665
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2571    4325    1239 
   Size:       1332    1754    2579

  Fragments arranged by size: 

                2579    1754    1332
 
Bpu10I CC'TnA_GC

Cuts at:   2532    2532 
   Size:       5665
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2392    2499    3790    4655    4953    2392 
   Size:        107    1291     865     298    3104

  Fragments arranged by size: 

                3104    1291     865     298     107
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2481    2756    4328     882 
   Size:        617     982     275    1572    2219

  Fragments arranged by size: 

                2219    1572     982     617     275
 
BsaAI yAC'GTr

Cuts at:    493    1204    3456     493 
   Size:        711    2252    2702

  Fragments arranged by size: 

                2702    2252     711
 
BsaBI GATnn'nnATC

Cuts at:   5659    5659 
   Size:       5665
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3472    3854    4489     275 
   Size:         53      83     186     936    1939     382     635    1451

  Fragments arranged by size: 

                1939    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4098    4496    4770    4917    5328    4098 
   Size:        398     274     147     411    4435

  Fragments arranged by size: 

                4435     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5440

  Fragments arranged by size: 

                5440     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2123    1186 
   Size:        937    4728
 
BseYI C'CCAG_C

Cuts at:   2789    4868    5364    2789 
   Size:       2079     496    3090

  Fragments arranged by size: 

                3090    2079     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5092
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3115    3876    4904    1540 
   Size:        275     186    1114     761    1028    2301

  Fragments arranged by size: 

                2301    1114    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3726    3811    4882    3726 
   Size:         85    1071    4509

  Fragments arranged by size: 

                4509    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3176    3269    3176 
   Size:         93    5572
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2102    2984    3726    3811    4882    1391 
   Size:        399     312     882     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     882     742     399     312      85
 
BspEI T'CCGG_A

Cuts at:   5328    5328 
   Size:       5665
 
BspHI T'CATG_A

Cuts at:   3554    5284    3554 
   Size:       1730    3935
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2755    3020     844 
   Size:       1911     265    3489

  Fragments arranged by size: 

                3489    1911     265
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2056    3552    1394 
   Size:        429     233    1496    3507

  Fragments arranged by size: 

                3507    1496     429     233
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4160    4334      65 
   Size:       1183    2912     174    1396

  Fragments arranged by size: 

                2912    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4309    4496    1700 
   Size:        110    2499     187    2869

  Fragments arranged by size: 

                2869    2499     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5665
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5665
 
BssSI C'ACGA_G

Cuts at:   3725    4737    3725 
   Size:       1012    4653
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5665
 
BstBI TT'CG_AA

Cuts at:   2737    4469    2737 
   Size:       1732    3933
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5665
 
BstYI r'GATC_y

Cuts at:   1972    2194    2995    3362    3747    3764    5216    5302    5314 
   Size:        222     801     367     385      17    1452      86      12

Cuts at:   5314    5660    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     801     385     367     346     222      86
                  17      12
 
BstZ17I GTA'TAC

Cuts at:   2744    2744 
   Size:       5665
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4174

  Fragments arranged by size: 

                4174     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4926

  Fragments arranged by size: 

                4926     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3181    3975    3995    3181 
   Size:        794      20    4851

  Fragments arranged by size: 

                4851     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5665
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5630
 
DraI TTT'AAA

Cuts at:   2969    2969 
   Size:       5665
 
DraIII CAC_nnn'GTG

Cuts at:   2609    2609 
   Size:       5665
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4672     809 
   Size:        507    3356    1802

  Fragments arranged by size: 

                3356    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3112    4001    5630 
   Size:         54    1425      50     275      18    1282     889    1629

Cuts at:   5630       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1282     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3112    1537 
   Size:        275    1300    4090

  Fragments arranged by size: 

                4090    1300     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2079    2943    3595    1353 
   Size:        726     864     652    3423

  Fragments arranged by size: 

                3423     864     726     652
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4252    4626    4772     387 
   Size:       1064     138    2663     374     146    1280

  Fragments arranged by size: 

                2663    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2382    2424    2459    2571    3728    4325    5112 
   Size:        393     750      42      35     112    1157     597     787

Cuts at:   5112    5380    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1157     787     750     597     393     268     112
                  42      35
 
EcoRI G'AATT_C

Cuts at:   2974    3352    2974 
   Size:        378    5287
 
FspI TGC'GCA

Cuts at:   4171    4171 
   Size:       5665
 
HaeII r_GCGC'y

Cuts at:   1678    2155    4812    1678 
   Size:        477    2657    2531

  Fragments arranged by size: 

                2657    2531     477
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2198    2230    2785    2817    4306 
   Size:         32     135      32     626      32     555      32    1489

Cuts at:   4306    4338    4380    4412    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1489     626     555     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2130    3009    3255     669 
   Size:        988     281     192     879     246    3079

  Fragments arranged by size: 

                3079     988     879     281     246     192
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5665
 
HpaI GTT'AAC

Cuts at:   3255    3255 
   Size:       5665
 
KpnI G_GTAC'C

Cuts at:   2990    2990 
   Size:       5665
 
MfeI C'AATT_G

Cuts at:   3264    3264 
   Size:       5665
 
MluI A'CGCG_T

Cuts at:   5541    5541 
   Size:       5665
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4779    4963    1305 
   Size:       3474     184    2007

  Fragments arranged by size: 

                3474    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3843

  Fragments arranged by size: 

                3843    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3623    3982    4141     518 
   Size:        648    2457     359     159    2042

  Fragments arranged by size: 

                2457    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2455    3081    3758    4906    5151    1606 
   Size:        849     626     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     849     677     626     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4174

  Fragments arranged by size: 

                4174     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5665
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5665
 
NotI GC'GGCC_GC

Cuts at:   3112    3112 
   Size:       5665
 
NspI r_CATG'y

Cuts at:   1132    1229    2300    3023    4568    1132 
   Size:         97    1071     723    1545    2229

  Fragments arranged by size: 

                2229    1545    1071     723      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5665
 
PciI A'CATG_T

Cuts at:   4564    4564 
   Size:       5665
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5665
 
PfoI T'CCnGG_A

Cuts at:   2828    2828 
   Size:       5665
 
PmeI GTTT'AAAC

Cuts at:   2969    2969 
   Size:       5665
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3734    3766    5247    5279    3734 
   Size:         32    1481      32    4120

  Fragments arranged by size: 

                4120    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5665
 
PsiI TTA'TAA

Cuts at:   3235    3235 
   Size:       5665
 
PstI C_TGCA'G

Cuts at:    830    2170    3017     830 
   Size:       1340     847    3478

  Fragments arranged by size: 

                3478    1340     847
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5665
 
SalI G'TCGA_C

Cuts at:   1936    3007    1936 
   Size:       1071    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2943    2943 
   Size:       5665
 
SbfI CC_TGCA'GG

Cuts at:   3017    3017 
   Size:       5665
 
ScaI AGT'ACT

Cuts at:   2331    3913    5625    2331 
   Size:       1582    1712    2371

  Fragments arranged by size: 

                2371    1712    1582
 
SfcI C'TryA_G

Cuts at:    826    1045    2166    3013    4148    4829    5020     826 
   Size:        219    1121     847    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1121     847     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5665
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5665
 
SmaI CCC'GGG

Cuts at:   1872    2992    1872 
   Size:       1120    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2407    2514    3769    4670    4932     820 
   Size:        197     926     464     107    1255     901     262    1553

  Fragments arranged by size: 

                1553    1255     926     901     464     262     197     107
            
 
SnaBI TAC'GTA

Cuts at:    493    3456     493 
   Size:       2963    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5665
 
SphI G_CATG'C

Cuts at:   1132    3023    1132 
   Size:       1891    3774
 
SspI AAT'ATT

Cuts at:      5      52    3589       5 
   Size:         47    3537    2081

  Fragments arranged by size: 

                3537    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5665
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3104     513 
   Size:        552     939    1100    3074

  Fragments arranged by size: 

                3074    1100     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3692    3709    3862    4047    4492     325 
   Size:       1255    2112      17     153     185     445    1498

  Fragments arranged by size: 

                2112    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2329    2640    3911 
   Size:        274      80      33      51    1443     352     311    1271

Cuts at:   3911    5623      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1271     352     311     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5625    5657    5625 
   Size:         32    5633
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5665
 
XbaI T'CTAG_A

Cuts at:   3001    3001 
   Size:       5665
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5665
 
XmaI C'CCGG_G

Cuts at:   1870    2990    1870 
   Size:       1120    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2073    3794    2073 
   Size:       1721    3944
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3473     276 
   Size:         53      83     186     936    1939    2468

  Fragments arranged by size: 

                2468    1939     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI   Bsu36I  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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