(Circular) (Six-base) MAPSORT of: pFN21AE0348.seq Check: 9246 from: 1 to: 6214 pFN21AE0348 6214 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2251 2843 2851 2976 2251 Size: 592 8 125 5489 Fragments arranged by size: 5489 592 125 8 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2916 3399 3557 1937 Size: 979 483 158 4594 Fragments arranged by size: 4594 979 483 158 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2561 4277 5661 5929 1632 Size: 695 234 1716 1384 268 1917 Fragments arranged by size: 1917 1716 1384 695 268 234 AfeI AGC'GCT Cuts at: 2891 2891 Size: 6214 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 5113 6090 1203 Size: 3910 977 1327 Fragments arranged by size: 3910 1327 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2284 2305 2473 2548 2617 2788 3957 5529 2284 Size: 21 168 75 69 171 1169 1572 2969 Fragments arranged by size: 2969 1572 1169 171 168 75 69 21 ApaI G_GGCC'C Cuts at: 3464 3464 Size: 6214 ApaLI G'TGCA_C Cuts at: 3104 4271 5427 3104 Size: 1167 1156 3891 Fragments arranged by size: 3891 1167 1156 ApoI r'AATT_y Cuts at: 1493 3025 3523 3749 3901 6010 1493 Size: 1532 498 226 152 2109 1697 Fragments arranged by size: 2109 1697 1532 498 226 152 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 2597 3539 1870 Size: 73 654 942 4545 Fragments arranged by size: 4545 942 654 73 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2408 2802 3017 3258 3535 Size: 325 217 627 621 394 215 241 277 Cuts at: 3535 4990 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 621 394 325 277 241 217 215 BanII G_rGCy'C Cuts at: 1391 2166 3464 3533 1391 Size: 775 1298 69 4072 Fragments arranged by size: 4072 1298 775 69 BbeI G_GCGC'C Cuts at: 3262 3262 Size: 6214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5243 BbvCI CC'TCA_GC Cuts at: 2654 2654 Size: 6214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3158 3192 4405 4439 1903 Size: 34 50 34 1137 34 1213 34 3678 Fragments arranged by size: 3678 1213 1137 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4825 136 Size: 107 122 71 631 605 3153 1525 Fragments arranged by size: 3153 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 2466 2684 3250 3631 2466 Size: 218 566 381 5049 Fragments arranged by size: 5049 566 381 218 Bme1580I G_kGCm'C Cuts at: 1790 2411 3108 3340 3464 4275 5431 1790 Size: 621 697 232 124 811 1156 2573 Fragments arranged by size: 2573 1156 811 697 621 232 124 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3166 3175 3945 Size: 416 53 83 399 24 1749 9 770 Cuts at: 3945 4903 442 Size: 958 1753 Fragments arranged by size: 1753 1749 958 770 416 399 83 53 24 9 BmtI G_CTAG'C Cuts at: 1056 2021 1056 Size: 965 5249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 2192 2294 2372 2552 2555 2639 2731 Size: 934 19 102 78 180 3 84 92 Cuts at: 2731 3372 4874 1239 Size: 641 1502 2579 Fragments arranged by size: 2579 1502 934 641 180 102 92 84 78 19 3 Bpu10I CC'TnA_GC Cuts at: 2654 2654 Size: 6214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2359 4339 5204 5502 2359 Size: 1980 865 298 3071 Fragments arranged by size: 3071 1980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2191 2504 2615 3060 4877 882 Size: 617 692 313 111 445 1817 2219 Fragments arranged by size: 2219 1817 692 617 445 313 111 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3259 3369 3396 4021 Size: 53 83 186 936 1726 110 27 625 Cuts at: 4021 4403 5038 275 Size: 382 635 1451 Fragments arranged by size: 1726 1451 936 635 625 382 186 110 83 53 27 BsaWI w'CCGG_w Cuts at: 4647 5045 5319 5466 5877 4647 Size: 398 274 147 411 4984 Fragments arranged by size: 4984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5989 Fragments arranged by size: 5989 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2651 1186 Size: 844 621 4749 Fragments arranged by size: 4749 844 621 BseYI C'CCAG_C Cuts at: 2013 2256 2404 3070 3074 3088 3464 5417 5913 Size: 243 148 666 4 14 376 1953 496 Cuts at: 5913 2013 Size: 2314 Fragments arranged by size: 2314 1953 666 496 376 243 148 14 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2898 1163 Size: 573 483 679 4479 Fragments arranged by size: 4479 679 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3084 3108 4275 4360 5431 3084 Size: 24 1167 85 1071 3867 Fragments arranged by size: 3867 1167 1071 85 24 BsmI GAATG_Cn' Cuts at: 3725 3818 3725 Size: 93 6121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2411 3084 3108 3340 3464 3533 Size: 399 376 245 673 24 232 124 69 Cuts at: 3533 4275 4360 5431 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 673 399 376 245 232 124 85 69 24 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2251 2347 2843 2851 2976 3569 844 Size: 1407 96 496 8 125 593 3489 Fragments arranged by size: 3489 1407 593 496 125 96 8 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3100 4709 4883 65 Size: 1183 1852 1609 174 1396 Fragments arranged by size: 1852 1609 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4858 5045 1700 Size: 110 3048 187 2869 Fragments arranged by size: 3048 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 2592 1925 Size: 667 5547 BssSI C'ACGA_G Cuts at: 3167 4274 5286 3167 Size: 1107 1012 4095 Fragments arranged by size: 4095 1107 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2284 2776 2809 794 Size: 1490 492 33 4199 Fragments arranged by size: 4199 1490 492 33 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstXI CCAn_nnnn'nTGG Cuts at: 2065 2533 2894 2065 Size: 468 361 5385 Fragments arranged by size: 5385 468 361 BstYI r'GATC_y Cuts at: 1972 2439 2883 3544 3911 4296 4313 5765 5851 Size: 467 444 661 367 385 17 1452 86 Cuts at: 5851 5863 6209 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 661 467 444 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2181 2181 Size: 6214 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5662 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2231 2282 2306 2430 2983 3211 3473 3730 4524 Size: 51 24 124 553 228 262 257 794 Cuts at: 4524 4544 2231 Size: 20 3901 Fragments arranged by size: 3901 794 553 262 257 228 124 51 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 2301 2809 3479 2301 Size: 508 670 5036 Fragments arranged by size: 5036 670 508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2518 5221 809 Size: 507 1202 2703 1802 Fragments arranged by size: 2703 1802 1202 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2223 2643 Size: 54 1425 50 275 18 295 98 420 Cuts at: 2643 3661 4550 6179 8 Size: 1018 889 1629 43 Fragments arranged by size: 1629 1425 1018 889 420 295 275 98 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4144 1353 Size: 2791 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2105 4801 5175 5321 387 Size: 1064 138 516 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 516 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2192 2294 2327 2372 2552 2555 Size: 393 541 19 102 33 45 180 3 Cuts at: 2555 2561 2639 2731 3372 4277 4874 5661 5929 Size: 6 78 92 641 905 597 787 268 Cuts at: 5929 1239 Size: 1524 Fragments arranged by size: 1524 905 787 641 597 541 393 268 180 102 92 78 45 33 19 6 3 EcoNI CCTnn'n_nnAGG Cuts at: 2756 2756 Size: 6214 EcoO109I rG'GnC_Cy Cuts at: 2177 2614 3460 3461 2177 Size: 437 846 1 4930 Fragments arranged by size: 4930 846 437 1 EcoRI G'AATT_C Cuts at: 3523 3901 3523 Size: 378 5836 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2353 2353 Size: 6214 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 2700 2893 3007 3262 5361 1678 Size: 1022 193 114 255 2099 2531 Fragments arranged by size: 2531 2099 1022 255 193 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4855 4887 4929 4961 1373 Size: 32 135 32 3283 32 42 32 2626 Fragments arranged by size: 3283 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3558 3804 669 Size: 988 281 1620 246 3079 Fragments arranged by size: 3079 1620 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KasI G'GCGC_C Cuts at: 3258 3258 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 5328 5512 1305 Size: 918 3105 184 2007 Fragments arranged by size: 3105 2007 918 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 2645 10 Size: 54 1425 343 393 420 3579 Fragments arranged by size: 3579 1425 420 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 2927 4172 4531 4690 518 Size: 648 897 864 1245 359 159 2042 Fragments arranged by size: 2042 1245 897 864 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 2545 2785 3034 3074 3246 3444 3630 Size: 666 273 240 249 40 172 198 186 Cuts at: 3630 4307 5455 5700 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 273 249 245 240 198 186 172 40 NarI GG'CG_CC Cuts at: 3259 3259 Size: 6214 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5662 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NheI G'CTAG_C Cuts at: 1052 2017 1052 Size: 965 5249 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 3572 5117 1132 Size: 97 2343 1545 2229 Fragments arranged by size: 2343 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6214 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2788 1108 Size: 1680 4534 PfoI T'CCnGG_A Cuts at: 2868 2868 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2849 2881 4283 4315 5796 5828 2849 Size: 32 1402 32 1481 32 3235 Fragments arranged by size: 3235 1481 1402 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3321 1613 Size: 1708 4506 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PspOMI G'GGCC_C Cuts at: 3460 3460 Size: 6214 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3203 3235 3203 Size: 32 6182 PstI C_TGCA'G Cuts at: 830 2031 2289 2760 2973 3282 3566 830 Size: 1201 258 471 213 309 284 3478 Fragments arranged by size: 3478 1201 471 309 284 258 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2272 2545 2785 3074 2272 Size: 273 240 289 5412 Fragments arranged by size: 5412 289 273 240 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SbfI CC_TGCA'GG Cuts at: 2760 3566 2760 Size: 806 5408 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SexAI A'CCwGG_T Cuts at: 2805 2805 Size: 6214 SfcI C'TryA_G Cuts at: 826 1045 2027 2131 2285 2552 2756 2969 3278 Size: 219 982 104 154 267 204 213 309 Cuts at: 3278 3562 4697 5378 5569 826 Size: 284 1135 681 191 1471 Fragments arranged by size: 1471 1135 982 681 309 284 267 219 213 204 191 154 104 SfoI GGC'GCC Cuts at: 3260 3260 Size: 6214 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 2597 4318 5219 5481 820 Size: 197 926 395 259 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 395 262 259 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 3173 1517 Size: 1656 4558 StyI C'CwwG_G Cuts at: 513 1065 3653 513 Size: 552 2588 3074 Fragments arranged by size: 3074 2588 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2468 4241 4258 4411 4596 5041 Size: 1255 690 198 1773 17 153 185 445 Cuts at: 5041 325 Size: 1498 Fragments arranged by size: 1773 1498 1255 690 445 198 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3423 4460 6172 Size: 274 80 33 51 1443 1446 1037 1712 Cuts at: 6172 96 Size: 138 Fragments arranged by size: 1712 1446 1443 1037 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 6174 6206 2100 Size: 32 4042 32 2108 Fragments arranged by size: 4042 2108 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6214 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2062 2401 2530 3347 2062 Size: 339 129 817 4929 Fragments arranged by size: 4929 817 339 129 XhoI C'TCGA_G Cuts at: 1943 2597 1943 Size: 654 5560 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 2957 4343 2957 Size: 1386 4828 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI