Restriction map for
pFN21AB7288
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BcgI BciVI
BclI BfrI BlnI BsaBI BsiWI
BspDI BspLU11I BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
HindIII HpaI KpnI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI ScaI SexAI SfiI SfuI
SpeI StuI SwaI Tsp509I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 3350 ACGTGT A^C[GA][CT]GT
AviII 185 TGCGCA TGC^GCA
BalI 1230 TGGCCA TGG^CCA
BamHI 3 GGATCC G^GATCC
BbsI 2125 GAAGAC GAAGAC(2/6)
BglII 3167 AGATCT A^GATCT
BpuAI 2125 GAAGAC GAAGAC(2/6)
Bsp1407I 3284 TGTACA T^GTACA
BspHI 881 TCATGA T^CATGA
BsrGI 3284 TGTACA T^GTACA
BstAPI 2714 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2192 GGTCACC G^GT[ACGT]ACC
BstPI 2192 GGTCACC G^GT[ACGT]ACC
DraI 1636 TTTAAA TTT^AAA
DraIII 217 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2403 CGGCCG C^GGCCG
EclXI 2403 CGGCCG C^GGCCG
Eco52I 2403 CGGCCG C^GGCCG
EcoO65I 2192 GGTCACC G^GT[ACGT]ACC
FseI 483 GGCCGGCC GGCCGG^CC
FspI 185 TGCGCA TGC^GCA
HincII 2527 GTCGAC GT[CT]^[GA]AC
HindII 2527 GTCGAC GT[CT]^[GA]AC
KspI 3011 CCGCGG CCGC^GG
MluNI 1230 TGGCCA TGG^CCA
MscI 1230 TGGCCA TGG^CCA
PaeR7I 756 CTCGAG C^TCGAG
RcaI 881 TCATGA T^CATGA
SacII 3011 CCGCGG CCGC^GG
SalI 2527 GTCGAC G^TCGAC
SapI 3111 GAAGAGC GCTCTTC(1/4)
SgrAI 8 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 756 CTCGAG C^T[CT][GA]AG
SnaBI 3118 TACGTA TAC^GTA
SspBI 3284 TGTACA T^GTACA
SspI 3204 AATATT AAT^ATT
TfiI 3581 GAATC G^A[AT]TC
XhoI 756 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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BbeI 393 GGCGCC GGCGC^C
1305 GGCGCC
BbrPI 217 CACGTG CAC^GTG
1234 CACGTG
BbvCI 714 CCTCAGC CCTCAGC(-5/-2)
1202 CCTCAGC
BfaI 490 CTAG C^TAG
736 CTAG
BglI 144 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
256 GCCCGCAGGGC
BsiEI 2403 CGGCCG CG[GA][CT]^CG
3547 CGACCG
BsmI 2734 GAATGC GAATGC(1/-1)
2825 GCATTC
BssSI 1936 CACGAG CACGAG(-5/-1)
388 CTCGTG
CpoI 272 CGGTCCG CG^G[AT]CCG
1163 CGGTCCG
EcoNI 1311 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2544 CCTGGAAGAGG
KasI 393 GGCGCC G^GCGCC
1305 GGCGCC
MaeI 490 CTAG C^TAG
736 CTAG
MseI 1637 TTAA T^TAA
2323 TTAA
NarI 393 GGCGCC GG^CGCC
1305 GGCGCC
PmaCI 217 CACGTG CAC^GTG
1234 CACGTG
PmlI 217 CACGTG CAC^GTG
1234 CACGTG
RsrII 272 CGGTCCG CG^G[AT]CCG
1163 CGGTCCG
SacI 2917 GAGCTC GAGCT^C
3589 GAGCTC
SbfI 2233 CCTGCAGG CCTGCA^GG
3065 CCTGCAGG
SfoI 393 GGCGCC GGC^GCC
1305 GGCGCC
Sse8387I 2233 CCTGCAGG CCTGCA^GG
3065 CCTGCAGG
Tru9I 1637 TTAA T^TAA
2323 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AvaI AvaII
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MroI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SphI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI