Restriction map for
pFN21AB7288
(Linear) (Six-base) MAPSORT of: KIBB7288.seq Check: 2190 from: 1 to: 3633
KIBB7288.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 371 1804 2258 2796 3633
Size: 371 1433 454 538 837
Fragments arranged by size:
1433 837 538 454 371
AccI GT'mk_AC
Cuts at: 0 2267 2528 2564 3633
Size: 2267 261 36 1069
Fragments arranged by size:
2267 1069 261 36
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2040 2599 3633
Size: 2040 559 1034
Fragments arranged by size:
2040 1034 559
AflIII A'CryG_T
Cuts at: 0 3350 3633
Size: 3350 283
AleI CACnn'nnGTG
Cuts at: 0 439 1738 3301 3633
Size: 439 1299 1563 332
Fragments arranged by size:
1563 1299 439 332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1151 1964 2824 3633
Size: 1151 813 860 809
Fragments arranged by size:
1151 860 813 809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 3633
Size: 547 32 3054
Fragments arranged by size:
3054 547 32
AlwNI CAG_nnn'CTG
Cuts at: 0 521 678 1014 1365 2063 2162 3132 3633
Size: 521 157 336 351 698 99 970 501
Fragments arranged by size:
970 698 521 501 351 336 157 99
ApaI G_GGCC'C
Cuts at: 0 146 1595 2431 2577 2948 3633
Size: 146 1449 836 146 371 685
Fragments arranged by size:
1449 836 685 371 146 146
ApaLI G'TGCA_C
Cuts at: 0 1506 1616 2860 3633
Size: 1506 110 1244 773
Fragments arranged by size:
1506 1244 773 110
AvaI C'yCGr_G
Cuts at: 0 282 397 454 661 756 1443 1800 2477
Size: 282 115 57 207 95 687 357 677
Cuts at: 2477 2890 3633
Size: 413 743
Fragments arranged by size:
743 687 677 413 357 282 207 115
95 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 850 883 3633
Size: 850 33 2750
Fragments arranged by size:
2750 850 33
BamHI G'GATC_C
Cuts at: 0 3 3633
Size: 3 3630
BanI G'GyrC_C
Cuts at: 0 177 393 430 922 1305 2440 2980 3633
Size: 177 216 37 492 383 1135 540 653
Fragments arranged by size:
1135 653 540 492 383 216 177 37
BanII G_rGCy'C
Cuts at: 0 146 258 449 1595 2431 2577 2921 2948
Size: 146 112 191 1146 836 146 344 27
Cuts at: 2948 3556 3593 3633
Size: 608 37 40
Fragments arranged by size:
1146 836 608 344 191 146 146 112
40 37 27
BbeI G_GCGC'C
Cuts at: 0 397 1309 3633
Size: 397 912 2324
Fragments arranged by size:
2324 912 397
BbsI GAAGACnn'nnnn_
Cuts at: 0 2132 3633
Size: 2132 1501
BbvCI CC'TCA_GC
Cuts at: 0 715 1203 3633
Size: 715 488 2430
Fragments arranged by size:
2430 715 488
BglI GCCn_nnn'nGGC
Cuts at: 0 150 262 3633
Size: 150 112 3371
Fragments arranged by size:
3371 150 112
BglII A'GATC_T
Cuts at: 0 3167 3633
Size: 3167 466
BlpI GC'TnA_GC
Cuts at: 0 514 608 1366 3633
Size: 514 94 758 2267
Fragments arranged by size:
2267 758 514 94
Bme1580I G_kGCm'C
Cuts at: 0 146 155 182 433 771 927 1510 1595
Size: 146 9 27 251 338 156 583 85
Cuts at: 1595 1620 2182 2431 2577 2864 2948 3377 3633
Size: 25 562 249 146 287 84 429 256
Fragments arranged by size:
583 562 429 338 287 256 251 249
156 146 146 85 84 27 25 9
BmgBI CAC'GTC
Cuts at: 0 480 650 2340 3351 3633
Size: 480 170 1690 1011 282
Fragments arranged by size:
1690 1011 480 282 170
BmrI ACTGGGnnnn_n'
Cuts at: 0 2616 2949 3364 3633
Size: 2616 333 415 269
Fragments arranged by size:
2616 415 333 269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1036 2097 2323 3423 3439 3633
Size: 1027 9 1061 226 1100 16 194
Fragments arranged by size:
1100 1061 1027 226 194 16 9
Bpu10I CC'TnA_GC
Cuts at: 0 715 961 1203 3633
Size: 715 246 242 2430
Fragments arranged by size:
2430 715 246 242
BsaI GGTCTCn'nnnn_
Cuts at: 0 347 1981 2487 2594 3633
Size: 347 1634 506 107 1039
Fragments arranged by size:
1634 1039 506 347 107
BsaAI yAC'GTr
Cuts at: 0 219 858 1236 1723 3120 3633
Size: 219 639 378 487 1397 513
Fragments arranged by size:
1397 639 513 487 378 219
BsaHI Gr'CG_yC
Cuts at: 0 194 394 731 1306 1913 2051 2270 2882
Size: 194 200 337 575 607 138 219 612
Cuts at: 2882 3633
Size: 751
Fragments arranged by size:
751 612 607 575 337 219 200 194
138
BsaWI w'CCGG_w
Cuts at: 0 275 545 1161 1517 3161 3633
Size: 275 270 616 356 1644 472
Fragments arranged by size:
1644 616 472 356 275 270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 639 669 1183 1213 1366 1396
Size: 547 30 62 30 514 30 153 30
Cuts at: 1396 1987 2017 2371 2401 3633
Size: 591 30 354 30 1232
Fragments arranged by size:
1232 591 547 514 354 153 62 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 322 492 568 571 574 647 754
Size: 319 3 170 76 3 3 73 107
Cuts at: 754 1191 1885 3055 3633
Size: 437 694 1170 578
Fragments arranged by size:
1170 694 578 437 319 170 107 76
73 3 3 3
BseYI C'CCAG_C
Cuts at: 0 813 1031 1239 1290 1436 1688 1916 2741
Size: 813 218 208 51 146 252 228 825
Cuts at: 2741 3633
Size: 892
Fragments arranged by size:
892 825 813 252 228 218 208 146
51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 856 1158 1336 1363 1435 1569 3633
Size: 343 513 302 178 27 72 134 2064
Fragments arranged by size:
2064 513 343 302 178 134 72 27
BsiEI CG_ry'CG
Cuts at: 0 2406 3550 3633
Size: 2406 1144 83
Fragments arranged by size:
2406 1144 83
BsiHKAI G_wGCw'C
Cuts at: 0 746 857 1253 1510 1620 1907 1943 2599
Size: 746 111 396 257 110 287 36 656
Cuts at: 2599 2864 2921 2962 3002 3593 3633
Size: 265 57 41 40 591 40
Fragments arranged by size:
746 656 591 396 287 265 257 111
110 57 41 40 40 36
BsmI GAATG_Cn'
Cuts at: 0 2740 2825 3633
Size: 2740 85 808
Fragments arranged by size:
2740 808 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 470 2387 2404 3633
Size: 470 1917 17 1229
Fragments arranged by size:
1917 1229 470 17
Bsp1286I G_dGCh'C
Cuts at: 0 146 155 182 258 433 449 746 771
Size: 146 9 27 76 175 16 297 25
Cuts at: 771 857 927 1253 1510 1595 1620 1907 1943
Size: 86 70 326 257 85 25 287 36
Cuts at: 1943 2182 2431 2577 2599 2864 2921 2948 2962
Size: 239 249 146 22 265 57 27 14
Cuts at: 2962 3002 3377 3556 3593 3633
Size: 40 375 179 37 40
Fragments arranged by size:
375 326 297 287 265 257 249 239
179 175 146 146 86 85 76 70
57 40 40 37 36 27 27 25
25 22 16 14 9
BspEI T'CCGG_A
Cuts at: 0 275 545 1517 3161 3633
Size: 275 270 972 1644 472
Fragments arranged by size:
1644 972 472 275 270
BspHI T'CATG_A
Cuts at: 0 881 3633
Size: 881 2752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 365 371 878 1385 1804 2258 2796 3073
Size: 365 6 507 507 419 454 538 277
Cuts at: 3073 3633
Size: 560
Fragments arranged by size:
560 538 507 507 454 419 365 277
6
BsrBI CCG'CTC
Cuts at: 0 22 78 160 2855 2991 3633
Size: 22 56 82 2695 136 642
Fragments arranged by size:
2695 642 136 82 56 22
BsrDI GCAATG_nn'
Cuts at: 0 1477 2363 2883 3633
Size: 1477 886 520 750
Fragments arranged by size:
1477 886 750 520
BsrFI r'CCGG_y
Cuts at: 0 9 484 638 1147 1425 3633
Size: 9 475 154 509 278 2208
Fragments arranged by size:
2208 509 475 278 154 9
BsrGI T'GTAC_A
Cuts at: 0 3284 3633
Size: 3284 349
BssSI C'ACGA_G
Cuts at: 0 388 1936 3633
Size: 388 1548 1697
Fragments arranged by size:
1697 1548 388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2720 3633
Size: 2720 913
BstEII G'GTnAC_C
Cuts at: 0 2192 3633
Size: 2192 1441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 1228 2376 2788 3633
Size: 441 787 1148 412 845
Fragments arranged by size:
1148 845 787 441 412
BstYI r'GATC_y
Cuts at: 0 3 2228 3099 3167 3633
Size: 3 2225 871 68 466
Fragments arranged by size:
2225 871 466 68 3
BtgI C'CryG_G
Cuts at: 0 843 2369 2423 2436 2673 3011 3386 3501
Size: 843 1526 54 13 237 338 375 115
Cuts at: 3501 3633
Size: 132
Fragments arranged by size:
1526 843 375 338 237 132 115 54
13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 539 3633
Size: 539 3094
BtsI GCAGTG_nn'
Cuts at: 0 1644 1882 2160 2871 3183 3633
Size: 1644 238 278 711 312 450
Fragments arranged by size:
1644 711 450 312 278 238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1254 1289 2589 2624 3633
Size: 1254 35 1300 35 1009
Fragments arranged by size:
1300 1254 1009 35 35
DraI TTT'AAA
Cuts at: 0 1638 3633
Size: 1638 1995
DraIII CAC_nnn'GTG
Cuts at: 0 222 3633
Size: 222 3411
DrdI GACnn_nn'nnGTC
Cuts at: 0 2479 2539 2560 3633
Size: 2479 60 21 1073
Fragments arranged by size:
2479 1073 60 21
EaeI y'GGCC_r
Cuts at: 0 348 482 640 1230 1423 1778 2288 2403
Size: 348 134 158 590 193 355 510 115
Cuts at: 2403 2992 3397 3633
Size: 589 405 236
Fragments arranged by size:
590 589 510 405 355 348 236 193
158 134 115
EagI C'GGCC_G
Cuts at: 0 2403 3633
Size: 2403 1230
EarI CTCTTCn'nnn_
Cuts at: 0 331 2324 2543 3106 3633
Size: 331 1993 219 563 527
Fragments arranged by size:
1993 563 527 331 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 777 2627 2808 3633
Size: 777 1850 181 825
Fragments arranged by size:
1850 825 777 181
EcoICRI GAG'CTC
Cuts at: 0 2919 3591 3633
Size: 2919 672 42
Fragments arranged by size:
2919 672 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1036 2040 2097 2323 2599 3423 3439
Size: 1027 9 1004 57 226 276 824 16
Cuts at: 3439 3633
Size: 194
Fragments arranged by size:
1027 1004 824 276 226 194 57 16
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1315 2548 3633
Size: 1315 1233 1085
Fragments arranged by size:
1315 1233 1085
EcoO109I rG'GnC_Cy
Cuts at: 0 143 675 689 807 1591 1592 1751 1890
Size: 143 532 14 118 784 1 159 139
Cuts at: 1890 2428 2574 2618 2945 3062 3423 3633
Size: 538 146 44 327 117 361 210
Fragments arranged by size:
784 538 532 361 327 210 159 146
143 139 118 117 44 14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2070 2337 3633
Size: 2070 267 1296
Fragments arranged by size:
2070 1296 267
FseI GG_CCGG'CC
Cuts at: 0 488 3633
Size: 488 3145
FspI TGC'GCA
Cuts at: 0 187 3633
Size: 187 3446
FspAI rTGC'GCAy
Cuts at: 0 187 3633
Size: 187 3446
HaeII r_GCGC'y
Cuts at: 0 397 514 1309 1432 1697 3633
Size: 397 117 795 123 265 1936
Fragments arranged by size:
1936 795 397 265 123 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1183 1215 1634 1666 1987 2019 2237 2269
Size: 1183 32 419 32 321 32 218 32
Cuts at: 2269 3136 3168 3633
Size: 867 32 465
Fragments arranged by size:
1183 867 465 419 321 218 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2529 3633
Size: 2529 1104
KasI G'GCGC_C
Cuts at: 0 393 1305 3633
Size: 393 912 2328
Fragments arranged by size:
2328 912 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 3633
Size: 453 3180
MscI TGG'CCA
Cuts at: 0 1232 3633
Size: 1232 2401
MslI CAynn'nnrTG
Cuts at: 0 439 934 1150 1385 1738 1946 2218 2368
Size: 439 495 216 235 353 208 272 150
Cuts at: 2368 2374 2464 2733 2806 2876 3301 3349 3391
Size: 6 90 269 73 70 425 48 42
Cuts at: 3391 3633
Size: 242
Fragments arranged by size:
495 439 425 353 272 269 242 235
216 208 150 90 73 70 48 42
6
MspA1I CmG'CkG
Cuts at: 0 1031 1172 1271 1349 1542 1688 3013 3633
Size: 1031 141 99 78 193 146 1325 620
Fragments arranged by size:
1325 1031 620 193 146 141 99 78
NaeI GCC'GGC
Cuts at: 0 11 486 640 1427 3633
Size: 11 475 154 787 2206
Fragments arranged by size:
2206 787 475 154 11
NarI GG'CG_CC
Cuts at: 0 394 1306 3633
Size: 394 912 2327
Fragments arranged by size:
2327 912 394
NcoI C'CATG_G
Cuts at: 0 2369 2423 3386 3501 3633
Size: 2369 54 963 115 132
Fragments arranged by size:
2369 963 132 115 54
NgoMIV G'CCGG_C
Cuts at: 0 9 484 638 1425 3633
Size: 9 475 154 787 2208
Fragments arranged by size:
2208 787 475 154 9
NspI r_CATG'y
Cuts at: 0 628 781 1128 1155 2805 3633
Size: 628 153 347 27 1650 828
Fragments arranged by size:
1650 828 628 347 153 27
PasI CC'CwG_GG
Cuts at: 0 147 198 726 3633
Size: 147 51 528 2907
Fragments arranged by size:
2907 528 147 51
PflMI CCAn_nnn'nTGG
Cuts at: 0 1239 2286 2664 3328 3507 3633
Size: 1239 1047 378 664 179 126
Fragments arranged by size:
1239 1047 664 378 179 126
PfoI T'CCnGG_A
Cuts at: 0 2543 3633
Size: 2543 1090
PmlI CAC'GTG
Cuts at: 0 219 1236 3633
Size: 219 1017 2397
Fragments arranged by size:
2397 1017 219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 3633
Size: 547 32 3054
Fragments arranged by size:
3054 547 32
PpuMI rG'GwC_Cy
Cuts at: 0 807 1751 1890 2618 3062 3423 3633
Size: 807 944 139 728 444 361 210
Fragments arranged by size:
944 807 728 444 361 210 139
PspOMI G'GGCC_C
Cuts at: 0 142 1591 2427 2573 2944 3633
Size: 142 1449 836 146 371 689
Fragments arranged by size:
1449 836 689 371 146 142
PspXI vC'TCGA_Gb
Cuts at: 0 756 3633
Size: 756 2877
PstI C_TGCA'G
Cuts at: 0 1354 1831 2083 2238 2420 2911 3070 3633
Size: 1354 477 252 155 182 491 159 563
Fragments arranged by size:
1354 563 491 477 252 182 159 155
PvuII CAG'CTG
Cuts at: 0 1172 1542 1688 3633
Size: 1172 370 146 1945
Fragments arranged by size:
1945 1172 370 146
RsrII CG'GwC_CG
Cuts at: 0 273 1164 3633
Size: 273 891 2469
Fragments arranged by size:
2469 891 273
SacI G_AGCT'C
Cuts at: 0 2921 3593 3633
Size: 2921 672 40
Fragments arranged by size:
2921 672 40
SacII CC_GC'GG
Cuts at: 0 3014 3633
Size: 3014 619
SalI G'TCGA_C
Cuts at: 0 2527 3633
Size: 2527 1106
SanDI GG'GwC_CC
Cuts at: 0 2618 3633
Size: 2618 1015
SapI GCTCTTCn'nnn_
Cuts at: 0 3106 3633
Size: 3106 527
SbfI CC_TGCA'GG
Cuts at: 0 2238 3070 3633
Size: 2238 832 563
Fragments arranged by size:
2238 832 563
SfcI C'TryA_G
Cuts at: 0 1350 1827 2079 2234 2416 2907 3066 3210
Size: 1350 477 252 155 182 491 159 144
Cuts at: 3210 3380 3633
Size: 170 253
Fragments arranged by size:
1350 491 477 253 252 182 170 159
155 144
SfoI GGC'GCC
Cuts at: 0 395 1307 3633
Size: 395 912 2326
Fragments arranged by size:
2326 912 395
SgrAI Cr'CCGG_yG
Cuts at: 0 9 3633
Size: 9 3624
SmaI CCC'GGG
Cuts at: 0 399 1445 2479 3633
Size: 399 1046 1034 1154
Fragments arranged by size:
1154 1046 1034 399
SmlI C'TyrA_G
Cuts at: 0 756 3633
Size: 756 2877
SnaBI TAC'GTA
Cuts at: 0 3120 3633
Size: 3120 513
SphI G_CATG'C
Cuts at: 0 781 1128 2805 3633
Size: 781 347 1677 828
Fragments arranged by size:
1677 828 781 347
SspI AAT'ATT
Cuts at: 0 3206 3633
Size: 3206 427
StyI C'CwwG_G
Cuts at: 0 578 917 1598 1952 2369 2423 3189 3386
Size: 578 339 681 354 417 54 766 197
Cuts at: 3386 3501 3633
Size: 115 132
Fragments arranged by size:
766 681 578 417 354 339 197 132
115 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 660 910 2211 2998 3479 3543 3564 3633
Size: 660 250 1301 787 481 64 21 69
Fragments arranged by size:
1301 787 660 481 250 69 64 21
TatI w'GTAC_w
Cuts at: 0 1895 1970 2387 3284 3633
Size: 1895 75 417 897 349
Fragments arranged by size:
1895 897 417 349 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 639 671 1599 1631 1652 1684 2116 2148
Size: 639 32 928 32 21 32 432 32
Cuts at: 2148 3633
Size: 1485
Fragments arranged by size:
1485 928 639 432 32 32 32 32
21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2013 2283 2451 3384 3633
Size: 2013 270 168 933 249
Fragments arranged by size:
2013 933 270 249 168
XhoI C'TCGA_G
Cuts at: 0 756 3633
Size: 756 2877
XmaI C'CCGG_G
Cuts at: 0 397 1443 2477 3633
Size: 397 1046 1034 1156
Fragments arranged by size:
1156 1046 1034 397
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI FspAI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SfcI SfoI SgrAI SmaI
SmlI SnaBI SphI SspI StyI TaqII TatI TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BcgI BciVI BclI BfrBI
BmtI BplI BpuEI BsaBI BsiWI BssHII BstBI BstZ17I
Bsu36I ClaI EcoRI EcoRV HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PshAI PsiI PsrI PvuI ScaI SexAI SfiI
SgfI SpeI SrfI StuI SwaI Tth111I XbaI XmnI
ZraI