Restriction map for
pFN21AB7223
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI Tth111I TthHB8I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 594 ACATGT A^C[GA][CT]GT
AlwI 755 GGATC GGATC(4/5)
AlwNI 356 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 342 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 187 GTGCTC G[AT]GC[AT]^C
BalI 268 TGGCCA TGG^CCA
BanI 786 GGTGCC G^G[CT][GA]CC
BanII 210 GGGCTC G[GA]GC[CT]^C
BclI 1370 TGATCA T^GATCA
BcnI 1146 CCCGG CC^[GC]GG
BsaBI 1371 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 167 ACCGGA [AT]^CCGG[AT]
BsiHKAI 187 GTGCTC G[AT]GC[AT]^C
Bsp1407I 1090 TGTACA T^GTACA
BspLU11I 594 ACATGT A^CATGT
BsrDI 978 GCAATG GCAATG(2/0)
BsrGI 1090 TGTACA T^GTACA
BstEII 840 GGTAACC G^GT[ACGT]ACC
BstNI 1006 CCTGG CC^[AT]GG
BstPI 840 GGTAACC G^GT[ACGT]ACC
Cfr13I 1361 GGGCC G^G[ACGT]CC
EcoO65I 840 GGTAACC G^GT[ACGT]ACC
EcoRII 1006 CCTGG ^CC[AT]GG
EcoRV 1443 GATATC GAT^ATC
EcoT22I 1422 ATGCAT ATGCA^T
FbaI 1370 TGATCA T^GATCA
MaeII 280 ACGT A^CGT
MamI 1371 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 268 TGGCCA TGG^CCA
MscI 268 TGGCCA TGG^CCA
MvaI 1006 CCTGG CC^[AT]GG
NciI 1146 CCCGG CC^[GC]GG
NsiI 1422 ATGCAT ATGCA^T
NspI 594 ACATGT [GA]CATG^[CT]
PflMI 730 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 910 CTGCAG CTGCA^G
Sau96I 1361 GGGCC G^G[ACGT]CC
ScaI 1037 AGTACT AGT^ACT
SspBI 1090 TGTACA T^GTACA
SspI 154 AATATT AAT^ATT
Van91I 730 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 451 TCTAGA T^CTAGA
XmnI 342 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 849 CCGC CCGC(-3/-1)
1303 GCGG
BbsI 858 GAAGAC GAAGAC(2/6)
598 GTCTTC
BciVI 146 GGATAC GTATCC(6/5)
403 GTATCC
BfaI 452 CTAG C^TAG
1112 CTAG
BglII 236 AGATCT A^GATCT
1329 AGATCT
BmyI 187 GTGCTC G[AGT]GC[ACT]^C
210 GGGCTC
BpuAI 858 GAAGAC GAAGAC(2/6)
598 GTCTTC
BsaJI 735 CCTTGG C^C[ACGT][ACGT]GG
880 CCTTGG
BseRI 1466 CTCCTC GAGGAG(10/8)
1507 GAGGAG
BsmAI 217 GTCTC GTCTC(1/5)
670 GTCTC
Bsp1286I 187 GTGCTC G[AGT]GC[ACT]^C
210 GGGCTC
BssKI 1006 CCTGG ^CC[ACGT]GG
1146 CCCGG
BstF5I 846 CATCC GGATG(2/0)
985 GGATG
BstYI 236 AGATCT [GA]^GATC[CT]
1329 AGATCT
Cac8I 248 GCTTGC GC[ACGT]^[ACGT]GC
831 GCACGC
EaeI 268 TGGCCA [CT]^GGCC[GA]
1148 CGGCCA
EarI 694 CTCTTC CTCTTC(1/4)
677 GAAGAG
EcoT14I 735 CCTTGG C^C[AT][AT]GG
880 CCTTGG
FokI 846 CATCC GGATG(9/13)
985 GGATG
HindIII 58 AAGCTT A^AGCTT
1184 AAGCTT
Ksp632I 694 CTCTTC CTCTTC(1/4)
677 GAAGAG
MaeI 452 CTAG C^TAG
1112 CTAG
MflI 236 AGATCT [GA]^GATC[CT]
1329 AGATCT
MwoI 173 GCAAAATTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
923 GCAGCTACAGC
PleI 41 GACTC GAGTC(4/5)
1464 GACTC
ScrFI 1006 CCTGG CC^[ACGT]GG
1146 CCCGG
SfaNI 358 GATGC GCATC(5/9)
748 GATGC
StyI 735 CCTTGG C^C[AT][AT]GG
880 CCTTGG
XhoII 236 AGATCT [GA]^GATC[CT]
1329 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsiYI BslI BsmI BsrI DdeI
DpnII Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspA1I MspI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI