Restriction map for pFN21AB7223
 (Circular) (Six-base) MAPSORT of: pFN21AB7223.seq  Check: 4083  from: 1  to: 6220



pFN21AB7223  6220 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:59  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4030     278 
   Size:         53      83     186     936    2494    2468

  Fragments arranged by size: 

                2494    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3563    1937 
   Size:       1626    4594
 
Acc65I G'GTAC_C

Cuts at:   3541    3541 
   Size:       6220
 
AclI AA'CG_TT

Cuts at:   1563    4347    4720    1563 
   Size:       2784     373    3063

  Fragments arranged by size: 

                3063    2784     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2479    3031    3464    4283    5667    5935    1632 
   Size:        847     552     433     819    1384     268    1917

  Fragments arranged by size: 

                1917    1384     847     819     552     433     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6023
 
AflIII A'CryG_T

Cuts at:   1203    2599    5119    6096    1203 
   Size:       1396    2520     977    1327

  Fragments arranged by size: 

                2520    1396    1327     977
 
AgeI A'CCGG_T

Cuts at:   5051    5051 
   Size:       6220
 
AhdI GACnn_n'nnGTC

Cuts at:   4949    4949 
   Size:       6220
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6220
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6220
 
AlwNI CAG_nnn'CTG

Cuts at:   2366    3963    5535    2366 
   Size:       1597    1572    3051

  Fragments arranged by size: 

                3051    1597    1572
 
ApaLI G'TGCA_C

Cuts at:   4277    5433    4277 
   Size:       1156    5064
 
ApoI r'AATT_y

Cuts at:   1493    2181    2375    2507    2920    3080    3529    3755    3907 
   Size:        688     194     132     413     160     449     226     152

Cuts at:   3907    6016    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2109    1697     688     449     413     226     194     160
                 152     132
 
AseI AT'TA_AT

Cuts at:    160    1032    4774     160 
   Size:        872    3742    1606

  Fragments arranged by size: 

                3742    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6220
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3545    1870 
   Size:         73    1602    4545

  Fragments arranged by size: 

                4545    1602      73
 
BamHI G'GATC_C

Cuts at:   3550    3917    3550 
   Size:        367    5853
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2791    3541    4996     618 
   Size:        325     217     627    1004     750    1455    1842

  Fragments arranged by size: 

                1842    1455    1004     750     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2219    3539    1391 
   Size:        828    1320    4072

  Fragments arranged by size: 

                4072    1320     828
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2596    2870     928 
   Size:        971     697     274    4278

  Fragments arranged by size: 

                4278     971     697     274
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4411    4445    1903 
   Size:         34    2474      34    3678

  Fragments arranged by size: 

                3678    2474      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2145    2419    4112    5322    2145 
   Size:        274    1693    1210    3043

  Fragments arranged by size: 

                3043    1693    1210     274
 
BclI T'GATC_A

Cuts at:   1548    3375    1548 
   Size:       1827    4393
 
BfrBI ATG'CAT

Cuts at:   3429    3429 
   Size:       6220
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4831     136 
   Size:        107     122      71     631     605    3159    1525

  Fragments arranged by size: 

                3159    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2241    3334    6215    2241 
   Size:       1093    2881    2246

  Fragments arranged by size: 

                2881    2246    1093
 
BlpI GC'TnA_GC

Cuts at:   3637    3637 
   Size:       6220
 
Bme1580I G_kGCm'C

Cuts at:   1790    4281    5437    1790 
   Size:       2491    1156    2573

  Fragments arranged by size: 

                2573    2491    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6220
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3457    3951    4909 
   Size:        416      53      83     399      24    2040     494     958

Cuts at:   4909     442 
   Size:       1753

  Fragments arranged by size: 

                2040    1753     958     494     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6220
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4880    1239 
   Size:       3641    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4345    5210    5508    4345 
   Size:        865     298    5057

  Fragments arranged by size: 

                5057     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4883     882 
   Size:        617    3384    2219

  Fragments arranged by size: 

                3384    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4011     493 
   Size:        711    2807    2702

  Fragments arranged by size: 

                2807    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3380    6214    3380 
   Size:       2834    3386
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4027    4409    5044     275 
   Size:         53      83     186     936    2494     382     635    1451

  Fragments arranged by size: 

                2494    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2172    4653    5051    5325    5472    5883    2172 
   Size:       2481     398     274     147     411    2509

  Fragments arranged by size: 

                2509    2481     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5995

  Fragments arranged by size: 

                5995     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3462    3527    1186 
   Size:       2276      65    3879

  Fragments arranged by size: 

                3879    2276      65
 
BseYI C'CCAG_C

Cuts at:   2323    2734    2756    2967    3294    3349    5423    5919    2323 
   Size:        411      22     211     327      55    2074     496    2624

  Fragments arranged by size: 

                2624    2074     496     411     327     211      55      22
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5647
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3670    4431    5459    1540 
   Size:        275     186    1669     761    1028    2301

  Fragments arranged by size: 

                2301    1669    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2196    4281    4366    5437    2196 
   Size:       2085      85    1071    2979

  Fragments arranged by size: 

                2979    2085    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2720    2838    2900    2963    3731    3824    2720 
   Size:        118      62      63     768      93    5116

  Fragments arranged by size: 

                5116     768     118      93      63      62
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2196    2219    3539    4281    4366    5437    1391 
   Size:        399     406      23    1320     742      85    1071    2174

  Fragments arranged by size: 

                2174    1320    1071     742     406     399      85      23
            
 
BspEI T'CCGG_A

Cuts at:   5883    5883 
   Size:       6220
 
BspHI T'CATG_A

Cuts at:   4109    5839    4109 
   Size:       1730    4490
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3575     844 
   Size:       2731    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4107    1394 
   Size:        429    2284    3507

  Fragments arranged by size: 

                3507    2284     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2990    4715    4889      65 
   Size:       1183    1742    1725     174    1396

  Fragments arranged by size: 

                1742    1725    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4864    5051    1700 
   Size:        110    3054     187    2869

  Fragments arranged by size: 

                3054    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    3095      96 
   Size:       2999    3221
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6220
 
BssSI C'ACGA_G

Cuts at:   4280    5292    4280 
   Size:       1012    5208
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6220
 
BstBI TT'CG_AA

Cuts at:   5024    5024 
   Size:       6220
 
BstEII G'GTnAC_C

Cuts at:   1184    2845    1184 
   Size:       1661    4559
 
BstYI r'GATC_y

Cuts at:   1972    2241    3334    3550    3917    4302    4319    5771    5857 
   Size:        269    1093     216     367     385      17    1452      86

Cuts at:   5857    5869    6215    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1093     385     367     346     269     216
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4729

  Fragments arranged by size: 

                4729     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5481

  Fragments arranged by size: 

                5481     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2084    3736    4530    4550    2084 
   Size:       1652     794      20    3754

  Fragments arranged by size: 

                3754    1652     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6220
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6185
 
DraI TTT'AAA

Cuts at:   3524    3524 
   Size:       6220
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5227     809 
   Size:        507    3911    1802

  Fragments arranged by size: 

                3911    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2273    3153    3667 
   Size:         54    1425      50     275      18     443     880     514

Cuts at:   3667    4556    6185       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     880     514     443     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3667    1537 
   Size:        275    1855    4090

  Fragments arranged by size: 

                4090    1855     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2677    2705    4150    1353 
   Size:       1324      28    1445    3423

  Fragments arranged by size: 

                3423    1445    1324      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2843    4807    5181    5327     387 
   Size:       1064     138    1254    1964     374     146    1280

  Fragments arranged by size: 

                1964    1280    1254    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2479    3031    3464    4283    4880    5667    5935 
   Size:        393     847     552     433     819     597     787     268

Cuts at:   5935    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     847     819     787     597     552     433     393
                 268
 
EcoRI G'AATT_C

Cuts at:   3529    3907    3529 
   Size:        378    5842
 
EcoRV GAT'ATC

Cuts at:   3450    3450 
   Size:       6220
 
FspI TGC'GCA

Cuts at:   4726    4726 
   Size:       6220
 
HaeII r_GCGC'y

Cuts at:   1678    5367    1678 
   Size:       3689    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2857    2889    4861    4893    4935 
   Size:         32     135      32    1285      32    1972      32      42

Cuts at:   4935    4967    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1972    1285     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3564    3810     669 
   Size:        988     281    1626     246    3079

  Fragments arranged by size: 

                3079    1626     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2063    3189     748 
   Size:       1315    1126    3779

  Fragments arranged by size: 

                3779    1315    1126
 
HpaI GTT'AAC

Cuts at:   3810    3810 
   Size:       6220
 
KpnI G_GTAC'C

Cuts at:   3545    3545 
   Size:       6220
 
MfeI C'AATT_G

Cuts at:   3819    3819 
   Size:       6220
 
MluI A'CGCG_T

Cuts at:   6096    6096 
   Size:       6220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2532    5334    5518    1305 
   Size:       1227    2802     184    2007

  Fragments arranged by size: 

                2802    2007    1227     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2275      10 
   Size:         54    1425     343     443    3955

  Fragments arranged by size: 

                3955    1425     443     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    4178    4537    4696     518 
   Size:        648    3012     359     159    2042

  Fragments arranged by size: 

                3012    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2327    3051    3349    3636    4313    5461    5706    1606 
   Size:        721     724     298     287     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     724     721     677     298     287     245
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4729

  Fragments arranged by size: 

                4729     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6220
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6220
 
NotI GC'GGCC_GC

Cuts at:   3667    3667 
   Size:       6220
 
NsiI A_TGCA'T

Cuts at:   3431    3431 
   Size:       6220
 
NspI r_CATG'y

Cuts at:   1132    1229    2603    3578    5123    1132 
   Size:         97    1374     975    1545    2229

  Fragments arranged by size: 

                2229    1545    1374     975      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6220
 
PciI A'CATG_T

Cuts at:   2599    5119    2599 
   Size:       2520    3700
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2741    1108 
   Size:       1633    4587
 
PfoI T'CCnGG_A

Cuts at:   3010    3010 
   Size:       6220
 
PmeI GTTT'AAAC

Cuts at:   3524    3524 
   Size:       6220
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4289    4321    5802    5834    4289 
   Size:         32    1481      32    4675

  Fragments arranged by size: 

                4675    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6220
 
PsiI TTA'TAA

Cuts at:   3790    3790 
   Size:       6220
 
PstI C_TGCA'G

Cuts at:    830    2919    3572     830 
   Size:       2089     653    3478

  Fragments arranged by size: 

                3478    2089     653
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6220
 
PvuII CAG'CTG

Cuts at:   2327    3051    3349    2327 
   Size:        724     298    5198

  Fragments arranged by size: 

                5198     724     298
 
SalI G'TCGA_C

Cuts at:   1936    3562    1936 
   Size:       1626    4594
 
SbfI CC_TGCA'GG

Cuts at:   3572    3572 
   Size:       6220
 
ScaI AGT'ACT

Cuts at:   3044    4468    6180    3044 
   Size:       1424    1712    3084

  Fragments arranged by size: 

                3084    1712    1424
 
SfcI C'TryA_G

Cuts at:    826    1045    2367    2915    2932    3268    3568    4703    5384 
   Size:        219    1322     548      17     336     300    1135     681

Cuts at:   5384    5575     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1322    1135     681     548     336     300     219
                 191      17
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6220
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6220
 
SmaI CCC'GGG

Cuts at:   1872    3547    1872 
   Size:       1675    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4324    5225    5487     820 
   Size:        197     926    2381     901     262    1553

  Fragments arranged by size: 

                2381    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4011     493 
   Size:       3518    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6220
 
SphI G_CATG'C

Cuts at:   1132    3578    1132 
   Size:       2446    3774
 
SspI AAT'ATT

Cuts at:      5      52    2161    4144       5 
   Size:         47    2109    1983    2081

  Fragments arranged by size: 

                2109    2081    1983      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6220
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2740    2885    3659     513 
   Size:        552     939     736     145     774    3074

  Fragments arranged by size: 

                3074     939     774     736     552     145
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4247    4264    4417    4602    5047     325 
   Size:       1255    2667      17     153     185     445    1498

  Fragments arranged by size: 

                2667    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3042    3064    3095 
   Size:        274      80      33      51    1443    1065      22      31

Cuts at:   3095    4466    6178      96 
   Size:       1371    1712     138

  Fragments arranged by size: 

                1712    1443    1371    1065     274     138      80      51
                  33      31      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2393    2425    3013    3045    6180    6212    2393 
   Size:         32     588      32    3135      32    2401

  Fragments arranged by size: 

                3135    2401     588      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6220
 
XbaI T'CTAG_A

Cuts at:   2456    3556    2456 
   Size:       1100    5120
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6220
 
XmaI C'CCGG_G

Cuts at:   1870    3545    1870 
   Size:       1675    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2351    4349    2351 
   Size:       1998    4222
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4028     276 
   Size:         53      83     186     936    2494    2468

  Fragments arranged by size: 

                2494    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     PacI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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