(Circular) (Six-base) MAPSORT of: pFN21AB7223.seq Check: 4083 from: 1 to: 6220 pFN21AB7223 6220 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2479 3031 3464 4283 5667 5935 1632 Size: 847 552 433 819 1384 268 1917 Fragments arranged by size: 1917 1384 847 819 552 433 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2599 5119 6096 1203 Size: 1396 2520 977 1327 Fragments arranged by size: 2520 1396 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 2366 3963 5535 2366 Size: 1597 1572 3051 Fragments arranged by size: 3051 1597 1572 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2181 2375 2507 2920 3080 3529 3755 3907 Size: 688 194 132 413 160 449 226 152 Cuts at: 3907 6016 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 688 449 413 226 194 160 152 132 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3545 1870 Size: 73 1602 4545 Fragments arranged by size: 4545 1602 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2791 3541 4996 618 Size: 325 217 627 1004 750 1455 1842 Fragments arranged by size: 1842 1455 1004 750 627 325 217 BanII G_rGCy'C Cuts at: 1391 2219 3539 1391 Size: 828 1320 4072 Fragments arranged by size: 4072 1320 828 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2596 2870 928 Size: 971 697 274 4278 Fragments arranged by size: 4278 971 697 274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2145 2419 4112 5322 2145 Size: 274 1693 1210 3043 Fragments arranged by size: 3043 1693 1210 274 BclI T'GATC_A Cuts at: 1548 3375 1548 Size: 1827 4393 BfrBI ATG'CAT Cuts at: 3429 3429 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2241 3334 6215 2241 Size: 1093 2881 2246 Fragments arranged by size: 2881 2246 1093 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 4281 5437 1790 Size: 2491 1156 2573 Fragments arranged by size: 2573 2491 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3457 3951 4909 Size: 416 53 83 399 24 2040 494 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 2040 1753 958 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4880 1239 Size: 3641 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4345 5210 5508 4345 Size: 865 298 5057 Fragments arranged by size: 5057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 3380 6214 3380 Size: 2834 3386 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2172 4653 5051 5325 5472 5883 2172 Size: 2481 398 274 147 411 2509 Fragments arranged by size: 2509 2481 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5995 Fragments arranged by size: 5995 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3462 3527 1186 Size: 2276 65 3879 Fragments arranged by size: 3879 2276 65 BseYI C'CCAG_C Cuts at: 2323 2734 2756 2967 3294 3349 5423 5919 2323 Size: 411 22 211 327 55 2074 496 2624 Fragments arranged by size: 2624 2074 496 411 327 211 55 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5647 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 4281 4366 5437 2196 Size: 2085 85 1071 2979 Fragments arranged by size: 2979 2085 1071 85 BsmI GAATG_Cn' Cuts at: 2720 2838 2900 2963 3731 3824 2720 Size: 118 62 63 768 93 5116 Fragments arranged by size: 5116 768 118 93 63 62 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2219 3539 4281 4366 5437 1391 Size: 399 406 23 1320 742 85 1071 2174 Fragments arranged by size: 2174 1320 1071 742 406 399 85 23 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3575 844 Size: 2731 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2990 4715 4889 65 Size: 1183 1742 1725 174 1396 Fragments arranged by size: 1742 1725 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4864 5051 1700 Size: 110 3054 187 2869 Fragments arranged by size: 3054 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3095 96 Size: 2999 3221 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 2845 1184 Size: 1661 4559 BstYI r'GATC_y Cuts at: 1972 2241 3334 3550 3917 4302 4319 5771 5857 Size: 269 1093 216 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1093 385 367 346 269 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2084 3736 4530 4550 2084 Size: 1652 794 20 3754 Fragments arranged by size: 3754 1652 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2273 3153 3667 Size: 54 1425 50 275 18 443 880 514 Cuts at: 3667 4556 6185 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 880 514 443 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2677 2705 4150 1353 Size: 1324 28 1445 3423 Fragments arranged by size: 3423 1445 1324 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2843 4807 5181 5327 387 Size: 1064 138 1254 1964 374 146 1280 Fragments arranged by size: 1964 1280 1254 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2479 3031 3464 4283 4880 5667 5935 Size: 393 847 552 433 819 597 787 268 Cuts at: 5935 1239 Size: 1524 Fragments arranged by size: 1524 847 819 787 597 552 433 393 268 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 EcoRV GAT'ATC Cuts at: 3450 3450 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 5367 1678 Size: 3689 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2857 2889 4861 4893 4935 Size: 32 135 32 1285 32 1972 32 42 Cuts at: 4935 4967 1373 Size: 32 2626 Fragments arranged by size: 2626 1972 1285 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 2063 3189 748 Size: 1315 1126 3779 Fragments arranged by size: 3779 1315 1126 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 5334 5518 1305 Size: 1227 2802 184 2007 Fragments arranged by size: 2802 2007 1227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2275 10 Size: 54 1425 343 443 3955 Fragments arranged by size: 3955 1425 443 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4178 4537 4696 518 Size: 648 3012 359 159 2042 Fragments arranged by size: 3012 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2327 3051 3349 3636 4313 5461 5706 1606 Size: 721 724 298 287 677 1148 245 2120 Fragments arranged by size: 2120 1148 724 721 677 298 287 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NsiI A_TGCA'T Cuts at: 3431 3431 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2603 3578 5123 1132 Size: 97 1374 975 1545 2229 Fragments arranged by size: 2229 1545 1374 975 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 2599 5119 2599 Size: 2520 3700 PflMI CCAn_nnn'nTGG Cuts at: 1108 2741 1108 Size: 1633 4587 PfoI T'CCnGG_A Cuts at: 3010 3010 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PstI C_TGCA'G Cuts at: 830 2919 3572 830 Size: 2089 653 3478 Fragments arranged by size: 3478 2089 653 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2327 3051 3349 2327 Size: 724 298 5198 Fragments arranged by size: 5198 724 298 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 3044 4468 6180 3044 Size: 1424 1712 3084 Fragments arranged by size: 3084 1712 1424 SfcI C'TryA_G Cuts at: 826 1045 2367 2915 2932 3268 3568 4703 5384 Size: 219 1322 548 17 336 300 1135 681 Cuts at: 5384 5575 826 Size: 191 1471 Fragments arranged by size: 1471 1322 1135 681 548 336 300 219 191 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4324 5225 5487 820 Size: 197 926 2381 901 262 1553 Fragments arranged by size: 2381 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 2161 4144 5 Size: 47 2109 1983 2081 Fragments arranged by size: 2109 2081 1983 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2004 2740 2885 3659 513 Size: 552 939 736 145 774 3074 Fragments arranged by size: 3074 939 774 736 552 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4247 4264 4417 4602 5047 325 Size: 1255 2667 17 153 185 445 1498 Fragments arranged by size: 2667 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3042 3064 3095 Size: 274 80 33 51 1443 1065 22 31 Cuts at: 3095 4466 6178 96 Size: 1371 1712 138 Fragments arranged by size: 1712 1443 1371 1065 274 138 80 51 33 31 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2425 3013 3045 6180 6212 2393 Size: 32 588 32 3135 32 2401 Fragments arranged by size: 3135 2401 588 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 2456 3556 2456 Size: 1100 5120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 2351 4349 2351 Size: 1998 4222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI