Restriction map for
pFN21AA0822
(Linear) (Six-base) MAPSORT of: KIAA0822.seq Check: 7634 from: 1 to: 4743
KIAA0822.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3399 4743
Size: 3399 1344
AatII G_ACGT'C
Cuts at: 0 1293 4743
Size: 1293 3450
AccI GT'mk_AC
Cuts at: 0 233 2521 3732 4213 4633 4743
Size: 233 2288 1211 481 420 110
Fragments arranged by size:
2288 1211 481 420 233 110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 891 1330 1872 2263 3473 4132 4743
Size: 383 508 439 542 391 1210 659 611
Fragments arranged by size:
1210 659 611 542 508 439 391 383
AflII C'TTAA_G
Cuts at: 0 2013 4743
Size: 2013 2730
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 4743
Size: 775 32 3936
Fragments arranged by size:
3936 775 32
AlwNI CAG_nnn'CTG
Cuts at: 0 613 3090 4017 4321 4677 4743
Size: 613 2477 927 304 356 66
Fragments arranged by size:
2477 927 613 356 304 66
ApoI r'AATT_y
Cuts at: 0 105 343 1149 1203 1262 1417 2149 3587
Size: 105 238 806 54 59 155 732 1438
Cuts at: 3587 3687 4402 4743
Size: 100 715 341
Fragments arranged by size:
1438 806 732 715 341 238 155 105
100 59 54
AseI AT'TA_AT
Cuts at: 0 551 2267 2482 4743
Size: 551 1716 215 2261
Fragments arranged by size:
2261 1716 551 215
AvaI C'yCGr_G
Cuts at: 0 4228 4743
Size: 4228 515
AvrII C'CTAG_G
Cuts at: 0 2190 4743
Size: 2190 2553
BamHI G'GATC_C
Cuts at: 0 1821 3573 4743
Size: 1821 1752 1170
Fragments arranged by size:
1821 1752 1170
BanI G'GyrC_C
Cuts at: 0 4288 4743
Size: 4288 455
BanII G_rGCy'C
Cuts at: 0 1972 3033 4460 4661 4743
Size: 1972 1061 1427 201 82
Fragments arranged by size:
1972 1427 1061 201 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 769 897 3111 3167 4132 4743
Size: 769 128 2214 56 965 611
Fragments arranged by size:
2214 965 769 611 128 56
BbvCI CC'TCA_GC
Cuts at: 0 4045 4061 4322 4743
Size: 4045 16 261 421
Fragments arranged by size:
4045 421 261 16
BclI T'GATC_A
Cuts at: 0 1344 2627 4743
Size: 1344 1283 2116
Fragments arranged by size:
2116 1344 1283
BfrBI ATG'CAT
Cuts at: 0 633 718 1159 2707 4743
Size: 633 85 441 1548 2036
Fragments arranged by size:
2036 1548 633 441 85
BglI GCCn_nnn'nGGC
Cuts at: 0 4261 4743
Size: 4261 482
BglII A'GATC_T
Cuts at: 0 865 1500 4743
Size: 865 635 3243
Fragments arranged by size:
3243 865 635
Bme1580I G_kGCm'C
Cuts at: 0 4293 4743
Size: 4293 450
BmrI ACTGGGnnnn_n'
Cuts at: 0 867 1890 2821 4186 4743
Size: 867 1023 931 1365 557
Fragments arranged by size:
1365 1023 931 867 557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1750 4743
Size: 1750 2993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 3911 3973 4039 4444 4660 4675 4743
Size: 1397 2514 62 66 405 216 15 68
Fragments arranged by size:
2514 1397 405 216 68 66 62 15
Bpu10I CC'TnA_GC
Cuts at: 0 4045 4061 4168 4322 4743
Size: 4045 16 107 154 421
Fragments arranged by size:
4045 421 154 107 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4078 4743
Size: 4078 665
BsaI GGTCTCn'nnnn_
Cuts at: 0 1331 4743
Size: 1331 3412
BsaAI yAC'GTr
Cuts at: 0 1350 3243 4743
Size: 1350 1893 1500
Fragments arranged by size:
1893 1500 1350
BsaBI GATnn'nnATC
Cuts at: 0 1376 2180 4700 4743
Size: 1376 804 2520 43
Fragments arranged by size:
2520 1376 804 43
BsaHI Gr'CG_yC
Cuts at: 0 1290 4743
Size: 1290 3453
BsaWI w'CCGG_w
Cuts at: 0 1678 3052 4119 4743
Size: 1678 1374 1067 624
Fragments arranged by size:
1678 1374 1067 624
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 589 619 4743
Size: 589 30 4124
Fragments arranged by size:
4124 589 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 997 2558 4506 4627 4705 4714 4743
Size: 610 387 1561 1948 121 78 9 29
Fragments arranged by size:
1948 1561 610 387 121 78 29 9
BseYI C'CCAG_C
Cuts at: 0 1121 3038 4677 4743
Size: 1121 1917 1639 66
Fragments arranged by size:
1917 1639 1121 66
BsiEI CG_ry'CG
Cuts at: 0 4217 4743
Size: 4217 526
BsiHKAI G_wGCw'C
Cuts at: 0 452 2681 3033 4661 4743
Size: 452 2229 352 1628 82
Fragments arranged by size:
2229 1628 452 352 82
BsmI GAATG_Cn'
Cuts at: 0 437 633 4743
Size: 437 196 4110
Fragments arranged by size:
4110 437 196
Bsp1286I G_dGCh'C
Cuts at: 0 452 1972 2681 3033 4293 4460 4661 4743
Size: 452 1520 709 352 1260 167 201 82
Fragments arranged by size:
1520 1260 709 452 352 201 167 82
BspEI T'CCGG_A
Cuts at: 0 3052 4743
Size: 3052 1691
BspHI T'CATG_A
Cuts at: 0 1389 3233 4743
Size: 1389 1844 1510
Fragments arranged by size:
1844 1510 1389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 3399 4427 4635 4743
Size: 289 3110 1028 208 108
Fragments arranged by size:
3110 1028 289 208 108
BsrBI CCG'CTC
Cuts at: 0 3935 4743
Size: 3935 808
BsrDI GCAATG_nn'
Cuts at: 0 109 2279 2808 2993 3719 4743
Size: 109 2170 529 185 726 1024
Fragments arranged by size:
2170 1024 726 529 185 109
BsrFI r'CCGG_y
Cuts at: 0 988 4743
Size: 988 3755
BsrGI T'GTAC_A
Cuts at: 0 1046 3468 4743
Size: 1046 2422 1275
Fragments arranged by size:
2422 1275 1046
BssSI C'ACGA_G
Cuts at: 0 3795 4743
Size: 3795 948
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2803 4743
Size: 2803 1940
BstBI TT'CG_AA
Cuts at: 0 3158 3350 4743
Size: 3158 192 1393
Fragments arranged by size:
3158 1393 192
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 4743
Size: 189 4554
BstYI r'GATC_y
Cuts at: 0 865 1500 1821 2169 3573 4472 4743
Size: 865 635 321 348 1404 899 271
Fragments arranged by size:
1404 899 865 635 348 321 271
BstZ17I GTA'TAC
Cuts at: 0 234 2522 4743
Size: 234 2288 2221
Fragments arranged by size:
2288 2221 234
Bsu36I CC'TnA_GG
Cuts at: 0 3974 4477 4743
Size: 3974 503 266
Fragments arranged by size:
3974 503 266
BtgI C'CryG_G
Cuts at: 0 182 2525 4743
Size: 182 2343 2218
Fragments arranged by size:
2343 2218 182
BtsI GCAGTG_nn'
Cuts at: 0 172 1532 1570 2473 4743
Size: 172 1360 38 903 2270
Fragments arranged by size:
2270 1360 903 172 38
ClaI AT'CG_AT
Cuts at: 0 3011 4743
Size: 3011 1732
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3384 3419 4743
Size: 3384 35 1324
Fragments arranged by size:
3384 1324 35
DraIII CAC_nnn'GTG
Cuts at: 0 588 2762 4743
Size: 588 2174 1981
Fragments arranged by size:
2174 1981 588
DrdI GACnn_nn'nnGTC
Cuts at: 0 1289 4743
Size: 1289 3454
EaeI y'GGCC_r
Cuts at: 0 4343 4743
Size: 4343 400
EarI CTCTTCn'nnn_
Cuts at: 0 1 542 1891 1977 2361 2513 3533 3748
Size: 1 541 1349 86 384 152 1020 215
Cuts at: 3748 4730 4743
Size: 982 13
Fragments arranged by size:
1349 1020 982 541 384 215 152 86
13 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1939 4743
Size: 1939 2804
EcoICRI GAG'CTC
Cuts at: 0 3031 4659 4743
Size: 3031 1628 84
Fragments arranged by size:
3031 1628 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 891 1330 1397 1872 2263 3473 3911
Size: 383 508 439 67 475 391 1210 438
Cuts at: 3911 3973 4039 4132 4444 4660 4675 4743
Size: 62 66 93 312 216 15 68
Fragments arranged by size:
1210 508 475 439 438 391 383 312
216 93 68 67 66 62 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2189 4642 4729 4743
Size: 2189 2453 87 14
Fragments arranged by size:
2453 2189 87 14
EcoRI G'AATT_C
Cuts at: 0 105 343 1149 1417 3687 4743
Size: 105 238 806 268 2270 1056
Fragments arranged by size:
2270 1056 806 268 238 105
EcoRV GAT'ATC
Cuts at: 0 2008 4743
Size: 2008 2735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2131 4176 4743
Size: 2131 2045 567
Fragments arranged by size:
2131 2045 567
FspI TGC'GCA
Cuts at: 0 2418 4743
Size: 2418 2325
HaeII r_GCGC'y
Cuts at: 0 1411 3091 4743
Size: 1411 1680 1652
Fragments arranged by size:
1680 1652 1411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 1992 2024 2396 2428 2722 2754
Size: 589 32 1371 32 372 32 294 32
Cuts at: 2754 3558 3590 4195 4227 4331 4363 4743
Size: 804 32 605 32 104 32 380
Fragments arranged by size:
1371 804 605 589 380 372 294 104
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 28 1568 4214 4743
Size: 28 1540 2646 529
Fragments arranged by size:
2646 1540 529 28
HindIII A'AGCT_T
Cuts at: 0 1083 1636 2016 4568 4743
Size: 1083 553 380 2552 175
Fragments arranged by size:
2552 1083 553 380 175
MfeI C'AATT_G
Cuts at: 0 2420 4743
Size: 2420 2323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1778 4410 4743
Size: 1778 2632 333
Fragments arranged by size:
2632 1778 333
MscI TGG'CCA
Cuts at: 0 4345 4743
Size: 4345 398
MslI CAynn'nnrTG
Cuts at: 0 473 2758 2998 3232 3880 4743
Size: 473 2285 240 234 648 863
Fragments arranged by size:
2285 863 648 473 240 234
MspA1I CmG'CkG
Cuts at: 0 3728 4110 4743
Size: 3728 382 633
Fragments arranged by size:
3728 633 382
NcoI C'CATG_G
Cuts at: 0 182 2525 4743
Size: 182 2343 2218
Fragments arranged by size:
2343 2218 182
NsiI A_TGCA'T
Cuts at: 0 635 720 1161 2709 4743
Size: 635 85 441 1548 2034
Fragments arranged by size:
2034 1548 635 441 85
NspI r_CATG'y
Cuts at: 0 2801 3168 4743
Size: 2801 367 1575
Fragments arranged by size:
2801 1575 367
PflMI CCAn_nnn'nTGG
Cuts at: 0 1813 1902 3910 4311 4743
Size: 1813 89 2008 401 432
Fragments arranged by size:
2008 1813 432 401 89
PfoI T'CCnGG_A
Cuts at: 0 2589 4652 4743
Size: 2589 2063 91
Fragments arranged by size:
2589 2063 91
PmlI CAC'GTG
Cuts at: 0 1350 4743
Size: 1350 3393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2336 2368 4743
Size: 2336 32 2375
Fragments arranged by size:
2375 2336 32
PsiI TTA'TAA
Cuts at: 0 661 862 2744 3020 4532 4743
Size: 661 201 1882 276 1512 211
Fragments arranged by size:
1882 1512 661 276 211 201
PstI C_TGCA'G
Cuts at: 0 4103 4743
Size: 4103 640
PvuII CAG'CTG
Cuts at: 0 3728 4110 4743
Size: 3728 382 633
Fragments arranged by size:
3728 633 382
SacI G_AGCT'C
Cuts at: 0 3033 4661 4743
Size: 3033 1628 82
Fragments arranged by size:
3033 1628 82
SalI G'TCGA_C
Cuts at: 0 4212 4743
Size: 4212 531
SapI GCTCTTCn'nnn_
Cuts at: 0 542 1977 4730 4743
Size: 542 1435 2753 13
Fragments arranged by size:
2753 1435 542 13
SfcI C'TryA_G
Cuts at: 0 4099 4743
Size: 4099 644
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4261 4743
Size: 4261 482
SmlI C'TyrA_G
Cuts at: 0 2013 4093 4743
Size: 2013 2080 650
Fragments arranged by size:
2080 2013 650
SphI G_CATG'C
Cuts at: 0 2801 4743
Size: 2801 1942
StuI AGG'CCT
Cuts at: 0 757 4743
Size: 757 3986
StyI C'CwwG_G
Cuts at: 0 182 1067 1422 1947 2190 2525 4664 4743
Size: 182 885 355 525 243 335 2139 79
Fragments arranged by size:
2139 885 525 355 335 243 182 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3023 4318 4352 4669 4743
Size: 3023 1295 34 317 74
Fragments arranged by size:
3023 1295 317 74 34
TatI w'GTAC_w
Cuts at: 0 1046 2298 2880 2902 3107 3468 4616 4743
Size: 1046 1252 582 22 205 361 1148 127
Fragments arranged by size:
1252 1148 1046 582 361 205 127 22
Tth111I GACn'n_nGTC
Cuts at: 0 4131 4743
Size: 4131 612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4308 4743
Size: 4308 435
XmnI GAAnn'nnTTC
Cuts at: 0 979 2821 3650 4743
Size: 979 1842 829 1093
Fragments arranged by size:
1842 1093 979 829
ZraI GAC'GTC
Cuts at: 0 1291 4743
Size: 1291 3452
Enzymes that do cut:
AarI AatII AccI AcuI AflII AloI AlwNI ApoI
AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BclI BfrBI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PflMI PfoI PmlI PpiI PsiI PstI PvuII SacI
SalI SapI SfcI SfiI SmlI SphI StuI StyI
TaqII TatI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI
ApaI ApaLI AscI AsiSI BaeI BbeI BcgI BciVI
BlpI BmgBI BmtI BsgI BsiWI BsmBI BssHII BstEII
BtgZI DraI EagI EcoO109I FseI FspAI HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SanDI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TstI XbaI XhoI XmaI