Restriction map for
pFN21AB4605
(Circular) (Six-base) MAPSORT of: pFN21AB4605.seq Check: 4774 from: 1 to: 6121
pFN21AB4605 6121 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 2742 3183 4184 5568 5836 1632
Size: 938 172 441 1001 1384 268 1917
Fragments arranged by size:
1917 1384 1001 938 441 268 172
AflII C'TTAA_G
Cuts at: 820 1017 2825 820
Size: 197 1808 4116
Fragments arranged by size:
4116 1808 197
AflIII A'CryG_T
Cuts at: 1203 2389 5020 5997 1203
Size: 1186 2631 977 1327
Fragments arranged by size:
2631 1327 1186 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2643 3012 1184
Size: 1459 369 4293
Fragments arranged by size:
4293 1459 369
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 6089
AlwNI CAG_nnn'CTG
Cuts at: 3864 5436 3864
Size: 1572 4549
ApaLI G'TGCA_C
Cuts at: 3304 4178 5334 3304
Size: 874 1156 4091
Fragments arranged by size:
4091 1156 874
ApoI r'AATT_y
Cuts at: 1493 2730 3120 3430 3656 3808 5917 1493
Size: 1237 390 310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1237 390 310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 3230 3451 3818 3230
Size: 221 367 5533
Fragments arranged by size:
5533 367 221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3442 4897 618
Size: 325 217 627 1655 1455 1842
Fragments arranged by size:
1842 1655 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2662 2766 3440 1391
Size: 1271 104 674 4072
Fragments arranged by size:
4072 1271 674 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BbvCI CC'TCA_GC
Cuts at: 2502 2502
Size: 6121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2861 4013 5223 2861
Size: 1152 1210 3759
Fragments arranged by size:
3759 1210 1152
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2312 2783 6116 2312
Size: 471 3333 2317
Fragments arranged by size:
3333 2317 471
BlpI GC'TnA_GC
Cuts at: 2682 3538 2682
Size: 856 5265
Bme1580I G_kGCm'C
Cuts at: 1790 3308 4182 5338 1790
Size: 1518 874 1156 2573
Fragments arranged by size:
2573 1518 1156 874
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3852 4810 442
Size: 416 53 83 399 24 2435 958 1753
Fragments arranged by size:
2435 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2211 2985 4781 1239
Size: 804 168 774 1796 2579
Fragments arranged by size:
2579 1796 804 774 168
Bpu10I CC'TnA_GC
Cuts at: 2502 3332 2502
Size: 830 5291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2090 3928 4310 4945
Size: 53 83 186 936 557 1838 382 635
Cuts at: 4945 275
Size: 1451
Fragments arranged by size:
1838 1451 936 635 557 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3237 3241 3267 3271 1178
Size: 30 165 30 1834 4 26 4 4028
Fragments arranged by size:
4028 1834 165 30 30 26 4 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3402 1186
Size: 2216 3905
BseYI C'CCAG_C
Cuts at: 5324 5820 5324
Size: 496 5625
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2469 3102 1163
Size: 573 733 633 4182
Fragments arranged by size:
4182 733 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2662 3216 3308 4182 4267 5338 2662
Size: 554 92 874 85 1071 3445
Fragments arranged by size:
3445 1071 874 554 92 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2662 2766 3216 3308 3440 4182 4267
Size: 399 872 104 450 92 132 742 85
Cuts at: 4267 5338 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 872 742 450 399 132 104
92 85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 2541 4010 5740 2541
Size: 1469 1730 2922
Fragments arranged by size:
2922 1730 1469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3476 844
Size: 2632 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2059 2312 2622 2783 3230 3451 3818 4203
Size: 87 253 310 161 447 221 367 385
Cuts at: 4203 4220 5672 5758 5770 6116 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 447 385 367 346 310 253
221 161 87 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 2460 3637 4431 4451 2460
Size: 1177 794 20 4130
Fragments arranged by size:
4130 1177 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DraIII CAC_nnn'GTG
Cuts at: 3045 3045
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2297 2413 3568
Size: 54 1425 50 275 18 467 116 1155
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1155 889 467 275 116 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2475 3334 3379 4051 1353
Size: 1122 859 45 672 3423
Fragments arranged by size:
3423 1122 859 672 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2028 4708 5082 5228 387
Size: 1064 138 439 2680 374 146 1280
Fragments arranged by size:
2680 1280 1064 439 374 146 138
EcoICRI GAG'CTC
Cuts at: 2660 2660
Size: 6121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2211 2570 2742 2985 3183 4184
Size: 393 411 168 359 172 243 198 1001
Cuts at: 4184 4781 5568 5836 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1001 787 597 411 393 359 268
243 198 172 168
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3381 3413 4762 4794 4836
Size: 32 135 32 1809 32 1349 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2626 1809 1349 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2088 2404 2797 3240 3465 3711
Size: 988 281 150 316 393 443 225 246
Cuts at: 3711 669
Size: 3079
Fragments arranged by size:
3079 988 443 393 316 281 246 225
150
HindIII A'AGCT_T
Cuts at: 748 2243 2273 748
Size: 1495 30 4596
Fragments arranged by size:
4596 1495 30
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 2142 3720 2142
Size: 1578 4543
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3001 3067 5235 5419 1305
Size: 1696 66 2168 184 2007
Fragments arranged by size:
2168 2007 1696 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2415 10
Size: 54 1425 343 583 3716
Fragments arranged by size:
3716 1425 583 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4079 4438 4597 518
Size: 648 2913 359 159 2042
Fragments arranged by size:
2913 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2686 3537 4214 5362 5607 1606
Size: 1080 851 677 1148 245 2120
Fragments arranged by size:
2120 1148 1080 851 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 2393 3479 5024 1132
Size: 97 1164 1086 1545 2229
Fragments arranged by size:
2229 1545 1164 1086 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 2389 5020 2389
Size: 2631 3490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 2271 3691 2271
Size: 1420 4701
PstI C_TGCA'G
Cuts at: 830 3473 830
Size: 2643 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2686 2686
Size: 6121
SacI G_AGCT'C
Cuts at: 2662 2662
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SapI GCTCTTCn'nnn_
Cuts at: 2475 2475
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 3075 4369 6081 3075
Size: 1294 1712 3115
Fragments arranged by size:
3115 1712 1294
SexAI A'CCwGG_T
Cuts at: 2896 2896
Size: 6121
SfcI C'TryA_G
Cuts at: 826 1045 2532 3469 4604 5285 5476 826
Size: 219 1487 937 1135 681 191 1471
Fragments arranged by size:
1487 1471 1135 937 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2825 4225 5126 5388 820
Size: 197 926 882 1400 901 262 1553
Fragments arranged by size:
1553 1400 926 901 882 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 2233 2698 4045 5
Size: 47 2181 465 1347 2081
Fragments arranged by size:
2181 2081 1347 465 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 2638 3560 513
Size: 552 939 66 568 922 3074
Fragments arranged by size:
3074 939 922 568 552 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4148 4165 4318 4503 4948 325
Size: 1255 2568 17 153 185 445 1498
Fragments arranged by size:
2568 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2327 2715 3073
Size: 274 80 33 51 1443 350 388 358
Cuts at: 3073 4367 6079 96
Size: 1294 1712 138
Fragments arranged by size:
1712 1443 1294 388 358 350 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3241 3273 6081 6113 3241
Size: 32 2808 32 3249
Fragments arranged by size:
3249 2808 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI
XcmI