################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AcsI 340 GAATTT [GA]^AATT[CT] AlwNI 602 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 340 GAATTT [GA]^AATT[CT] AspI 248 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 247 GGACC G^G[AT]CC BcgI 132 GCATGAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 486 AGATCT A^GATCT BpmI 69 CTCCAG CTGGAG(16/14) BseRI 619 GAGGAG GAGGAG(10/8) BsiYI 540 CCGTTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 540 CCGTTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrDI 158 GCAATG GCAATG(2/0) BstYI 486 AGATCT [GA]^GATC[CT] Cac8I 198 GCCAGC GC[ACGT]^[ACGT]GC DraI 343 TTTAAA TTT^AAA EarI 571 CTCTTC CTCTTC(1/4) Ksp632I 571 CTCTTC CTCTTC(1/4) MaeII 348 ACGT A^CGT MflI 486 AGATCT [GA]^GATC[CT] MseI 344 TTAA T^TAA MspA1I 602 CAGCTG C[AC]G^C[GT]G PflFI 248 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 138 GACTC GAGTC(4/5) PvuII 602 CAGCTG CAG^CTG SmlI 378 CTCAAG C^T[CT][GA]AG TaqI 78 TCGA T^CGA Tru9I 344 TTAA T^TAA TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 248 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 78 TCGA T^CGA XcmI 114 CCAGAGCTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 486 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 27 GTAC GT^AC 371 GTAC AlwI 205 GGATC GGATC(4/5) 524 GGATC BsmAI 136 GAGAC GTCTC(1/5) 149 GAGAC Cfr13I 247 GGACC G^G[ACGT]CC 628 GGGCC HaeIII 538 GGCC GG^CC 629 GGCC MaeIII 407 GTTAC ^GT[ACGT]AC 413 GTAAC NlaIII 133 CATG CATG^ 226 CATG NlaIV 247 GGACCC GG[ACGT]^[ACGT]CC 627 GGGGCC PstI 331 CTGCAG CTGCA^G 585 CTGCAG RsaI 27 GTAC GT^AC 371 GTAC Sau96I 247 GGACC G^G[ACGT]CC 628 GGGCC SfaNI 235 GATGC GCATC(5/9) 361 GATGC SfcI 331 CTGCAG C^T[GA][CT]AG 585 CTGCAG TfiI 80 GATTC G^A[AT]TC 222 GAATC Tsp509I 341 AATT ^AATT 355 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BbvI BsrI DdeI DpnII Fnu4HI HinfI ItaI MboI MboII MnlI MwoI NdeII Sau3AI TseI