Restriction map for pFN21AE1082
 (Circular) (Six-base) MAPSORT of: pFN21AE1082.seq  Check: 896  from: 1  to: 6022



pFN21AE1082  6022 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 11:00  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3832     278 
   Size:         53      83     186     936    2296    2468

  Fragments arranged by size: 

                2468    2296     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3365    1937 
   Size:       1428    4594
 
Acc65I G'GTAC_C

Cuts at:   3343    3343 
   Size:       6022
 
AclI AA'CG_TT

Cuts at:   1563    2016    4149    4522    1563 
   Size:        453    2133     373    3063

  Fragments arranged by size: 

                3063    2133     453     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3270    4085    5469    5737    1632 
   Size:       1638     815    1384     268    1917

  Fragments arranged by size: 

                1917    1638    1384     815     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5825
 
AflIII A'CryG_T

Cuts at:   1203    4921    5898    1203 
   Size:       3718     977    1327

  Fragments arranged by size: 

                3718    1327     977
 
AgeI A'CCGG_T

Cuts at:   4853    4853 
   Size:       6022
 
AhdI GACnn_n'nnGTC

Cuts at:   4751    4751 
   Size:       6022
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6022
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6022
 
AlwNI CAG_nnn'CTG

Cuts at:   2514    2545    2840    3765    5337    2514 
   Size:         31     295     925    1572    3199

  Fragments arranged by size: 

                3199    1572     925     295      31
 
ApaLI G'TGCA_C

Cuts at:   4079    5235    4079 
   Size:       1156    4866
 
ApoI r'AATT_y

Cuts at:   1493    2159    3331    3557    3709    5818    1493 
   Size:        666    1172     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1172     666     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4576     160 
   Size:        872    3544    1606

  Fragments arranged by size: 

                3544    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6022
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2708    3347    1870 
   Size:         73     765     639    4545

  Fragments arranged by size: 

                4545     765     639      73
 
BamHI G'GATC_C

Cuts at:   3352    3719    3352 
   Size:        367    5655
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2134    2901    3343    4798     618 
   Size:        325     217     627     347     767     442    1455    1842

  Fragments arranged by size: 

                1842    1455     767     627     442     347     325     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2211    2715    3341    1391 
   Size:        820     504     626    4072

  Fragments arranged by size: 

                4072     820     626     504
 
BbeI G_GCGC'C

Cuts at:   2138    2138 
   Size:       6022
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2578     928 
   Size:        971     679    4372

  Fragments arranged by size: 

                4372     971     679
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4213    4247    1903 
   Size:         34      50      34    2192      34    3678

  Fragments arranged by size: 

                3678    2192      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3914    5124    3914 
   Size:       1210    4812
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6022
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4633     136 
   Size:        107     122      71     631     605    2961    1525

  Fragments arranged by size: 

                2961    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6017    6017 
   Size:       6022
 
BlpI GC'TnA_GC

Cuts at:   3439    3439 
   Size:       6022
 
Bme1580I G_kGCm'C

Cuts at:   1790    4083    5239    1790 
   Size:       2293    1156    2573

  Fragments arranged by size: 

                2573    2293    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2908    1318 
   Size:       1590    4432
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3753    4711     442 
   Size:        416      53      83     399      24    2336     958    1753

  Fragments arranged by size: 

                2336    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6022
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2835    2835 
   Size:       6022
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2321    2509    2626    2877    4682    1239 
   Size:       1082     188     117     251    1805    2579

  Fragments arranged by size: 

                2579    1805    1082     251     188     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2879    4147    5012    5310    2879 
   Size:       1268     865     298    3591

  Fragments arranged by size: 

                3591    1268     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2363    4685     882 
   Size:        617     864    2322    2219

  Fragments arranged by size: 

                2322    2219     864     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3813     493 
   Size:        711    2609    2702

  Fragments arranged by size: 

                2702    2609     711
 
BsaBI GATnn'nnATC

Cuts at:   6016    6016 
   Size:       6022
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2135    3829    4211    4846 
   Size:         53      83     186     936     602    1694     382     635

Cuts at:   4846     275 
   Size:       1451

  Fragments arranged by size: 

                1694    1451     936     635     602     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4455    4853    5127    5274    5685    4455 
   Size:        398     274     147     411    4792

  Fragments arranged by size: 

                4792     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2319    2349    1178 
   Size:         30     165      30     916      30    4851

  Fragments arranged by size: 

                4851     916     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2099    2102    2340    2668    2744    2777    2865    3071 
   Size:        913       3     238     328      76      33      88     206

Cuts at:   3071    3115    3245    1186 
   Size:         44     130    3963

  Fragments arranged by size: 

                3963     913     328     238     206     130      88      76
                  44      33       3
 
BseYI C'CCAG_C

Cuts at:   2138    2320    5225    5721    2138 
   Size:        182    2905     496    2439

  Fragments arranged by size: 

                2905    2439     496     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2081    1163 
   Size:        573     345    5104

  Fragments arranged by size: 

                5104     573     345
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2292    2424    3472    4233    5261    1540 
   Size:        275     186     291     132    1048     761    1028    2301

  Fragments arranged by size: 

                2301    1048    1028     761     291     275     186     132
            
 
BsiHKAI G_wGCw'C

Cuts at:   2724    4083    4168    5239    2724 
   Size:       1359      85    1071    3507

  Fragments arranged by size: 

                3507    1359    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3533    3626    3533 
   Size:         93    5929
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2443    2914    2443 
   Size:        471    5551
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2211    2715    2724    3341    4083    4168    5239 
   Size:        399     421     504       9     617     742      85    1071

Cuts at:   5239    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     617     504     421     399      85
                   9
 
BspEI T'CCGG_A

Cuts at:   5685    5685 
   Size:       6022
 
BspHI T'CATG_A

Cuts at:   3911    5641    3911 
   Size:       1730    4292
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2544    3377     844 
   Size:       1700     833    3489

  Fragments arranged by size: 

                3489    1700     833
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2202    2412    3909    1394 
   Size:        429     379     210    1497    3507

  Fragments arranged by size: 

                3507    1497     429     379     210
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4517    4691      65 
   Size:       1183    3269     174    1396

  Fragments arranged by size: 

                3269    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4666    4853    1700 
   Size:        110    2856     187    2869

  Fragments arranged by size: 

                2869    2856     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6022
 
BssHII G'CGCG_C

Cuts at:   1925    2468    1925 
   Size:        543    5479
 
BssSI C'ACGA_G

Cuts at:   4082    5094    4082 
   Size:       1012    5010
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6022
 
BstBI TT'CG_AA

Cuts at:   4826    4826 
   Size:       6022
 
BstEII G'GTnAC_C

Cuts at:   1184    2308    1184 
   Size:       1124    4898
 
BstYI r'GATC_y

Cuts at:   1972    2542    3144    3352    3719    4104    4121    5573    5659 
   Size:        570     602     208     367     385      17    1452      86

Cuts at:   5659    5671    6017    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     602     570     385     367     346     208
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2286    2500     513 
   Size:        552    1221     214    4035

  Fragments arranged by size: 

                4035    1221     552     214
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5283

  Fragments arranged by size: 

                5283     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3538    4332    4352    3538 
   Size:        794      20    5208

  Fragments arranged by size: 

                5208     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2790    1554 
   Size:       1236    4786
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5987
 
DraI TTT'AAA

Cuts at:   3326    3326 
   Size:       6022
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5029     809 
   Size:        507    3713    1802

  Fragments arranged by size: 

                3713    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2289    2421    2503 
   Size:         54    1425      50     275      18     459     132      82

Cuts at:   2503    3469    4358    5987       8 
   Size:        966     889    1629      43

  Fragments arranged by size: 

                1629    1425     966     889     459     275     132      82
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2289    2421    3469    1537 
   Size:        275     477     132    1048    4090

  Fragments arranged by size: 

                4090    1048     477     275     132
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3246    3952    1353 
   Size:       1893     706    3423

  Fragments arranged by size: 

                3423    1893     706
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4609    4983    5129     387 
   Size:       1064     138    3020     374     146    1280

  Fragments arranged by size: 

                3020    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2321    2509    2626    2877    3270    4085    4682 
   Size:        393     689     188     117     251     393     815     597

Cuts at:   4682    5469    5737    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     815     787     689     597     393     393     268
                 251     188     117
 
EcoO109I rG'GnC_Cy

Cuts at:   3042    3172    3042 
   Size:        130    5892
 
EcoRI G'AATT_C

Cuts at:   3331    3709    3331 
   Size:        378    5644
 
EcoRV GAT'ATC

Cuts at:   2149    2149 
   Size:       6022
 
FspI TGC'GCA

Cuts at:   4528    4528 
   Size:       6022
 
HaeII r_GCGC'y

Cuts at:   1678    2138    2553    2649    2939    3097    5169    1678 
   Size:        460     415      96     290     158    2072    2531

  Fragments arranged by size: 

                2531    2072     460     415     290     158      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2039    2071    2597    2629    2828 
   Size:         32     135      32     467      32     526      32     199

Cuts at:   2828    2860    4663    4695    4737    4769    1373 
   Size:         32    1803      32      42      32    2626

  Fragments arranged by size: 

                2626    1803     526     467     199     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3366    3612     669 
   Size:        988     281    1428     246    3079

  Fragments arranged by size: 

                3079    1428     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6022
 
HpaI GTT'AAC

Cuts at:   3612    3612 
   Size:       6022
 
KasI G'GCGC_C

Cuts at:   2134    2134 
   Size:       6022
 
KpnI G_GTAC'C

Cuts at:   3347    3347 
   Size:       6022
 
MfeI C'AATT_G

Cuts at:   3621    3621 
   Size:       6022
 
MluI A'CGCG_T

Cuts at:   5898    5898 
   Size:       6022
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2964    3135    5136    5320    1305 
   Size:       1659     171    2001     184    2007

  Fragments arranged by size: 

                2007    2001    1659     184     171
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4200

  Fragments arranged by size: 

                4200    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3980    4339    4498     518 
   Size:        648    2814     359     159    2042

  Fragments arranged by size: 

                2814    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2299    2481    2502    3438    4115    5263    5508    1606 
   Size:        693     182      21     936     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     936     693     677     245     182      21
            
 
NarI GG'CG_CC

Cuts at:   2135    2135 
   Size:       6022
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5470
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6022
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6022
 
NotI GC'GGCC_GC

Cuts at:   3469    3469 
   Size:       6022
 
NspI r_CATG'y

Cuts at:   1132    1229    3380    4925    1132 
   Size:         97    2151    1545    2229

  Fragments arranged by size: 

                2229    2151    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6022
 
PciI A'CATG_T

Cuts at:   4921    4921 
   Size:       6022
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6022
 
PfoI T'CCnGG_A

Cuts at:   2394    3147    2394 
   Size:        753    5269
 
PmeI GTTT'AAAC

Cuts at:   3326    3326 
   Size:       6022
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4091    4123    5604    5636    4091 
   Size:         32    1481      32    4477

  Fragments arranged by size: 

                4477    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3042    3042 
   Size:       6022
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6022
 
PsiI TTA'TAA

Cuts at:   3592    3592 
   Size:       6022
 
PstI C_TGCA'G

Cuts at:    830    2127    2541    3374     830 
   Size:       1297     414     833    3478

  Fragments arranged by size: 

                3478    1297     833     414
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6022
 
PvuII CAG'CTG

Cuts at:   2299    2299 
   Size:       6022
 
SacII CC_GC'GG

Cuts at:   2503    2503 
   Size:       6022
 
SalI G'TCGA_C

Cuts at:   1936    3364    1936 
   Size:       1428    4594
 
SbfI CC_TGCA'GG

Cuts at:   2541    3374    2541 
   Size:        833    5189
 
ScaI AGT'ACT

Cuts at:   4270    5982    4270 
   Size:       1712    4310
 
SfcI C'TryA_G

Cuts at:    826    1045    2123    2537    3370    4505    5186    5377     826 
   Size:        219    1078     414     833    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1078     833     681     414     219     191
            
 
SfoI GGC'GCC

Cuts at:   2136    2136 
   Size:       6022
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6022
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6022
 
SmaI CCC'GGG

Cuts at:   1872    3349    1872 
   Size:       1477    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2894    4126    5027    5289     820 
   Size:        197     926     951    1232     901     262    1553

  Fragments arranged by size: 

                1553    1232     951     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3813     493 
   Size:       3320    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6022
 
SphI G_CATG'C

Cuts at:   1132    3380    1132 
   Size:       2248    3774
 
SspI AAT'ATT

Cuts at:      5      52    3946       5 
   Size:         47    3894    2081

  Fragments arranged by size: 

                3894    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6022
 
StyI C'CwwG_G

Cuts at:    513    1065    3177    3461     513 
   Size:        552    2112     284    3074

  Fragments arranged by size: 

                3074    2112     552     284
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2327    4049    4066    4219    4404    4849     325 
   Size:       1255     747    1722      17     153     185     445    1498

  Fragments arranged by size: 

                1722    1498    1255     747     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4268    5980      96 
   Size:        274      80      33      51    1443    2291    1712     138

  Fragments arranged by size: 

                2291    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2319    2351    2390    2422    3301    3333    5982    6014    2319 
   Size:         32      39      32     879      32    2649      32    2327

  Fragments arranged by size: 

                2649    2327     879      39      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2686    2990    1316 
   Size:       1370     304    4348

  Fragments arranged by size: 

                4348    1370     304
 
XbaI T'CTAG_A

Cuts at:   3358    3358 
   Size:       6022
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6022
 
XmaI C'CCGG_G

Cuts at:   1870    3347    1870 
   Size:       1477    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2159    4151    2159 
   Size:       1992    4030
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3830     276 
   Size:         53      83     186     936    2296    2468

  Fragments arranged by size: 

                2468    2296     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII    SacII     SalI     SbfI
     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI     FalI     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI
     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI    SanDI
     SapI    SexAI     SfiI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com