(Circular) (Six-base) MAPSORT of: pFN21AE1082.seq Check: 896 from: 1 to: 6022 pFN21AE1082 6022 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3365 1937 Size: 1428 4594 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 2016 4149 4522 1563 Size: 453 2133 373 3063 Fragments arranged by size: 3063 2133 453 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3270 4085 5469 5737 1632 Size: 1638 815 1384 268 1917 Fragments arranged by size: 1917 1638 1384 815 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AlwNI CAG_nnn'CTG Cuts at: 2514 2545 2840 3765 5337 2514 Size: 31 295 925 1572 3199 Fragments arranged by size: 3199 1572 925 295 31 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 2159 3331 3557 3709 5818 1493 Size: 666 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 666 226 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2708 3347 1870 Size: 73 765 639 4545 Fragments arranged by size: 4545 765 639 73 BamHI G'GATC_C Cuts at: 3352 3719 3352 Size: 367 5655 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 2901 3343 4798 618 Size: 325 217 627 347 767 442 1455 1842 Fragments arranged by size: 1842 1455 767 627 442 347 325 217 BanII G_rGCy'C Cuts at: 1391 2211 2715 3341 1391 Size: 820 504 626 4072 Fragments arranged by size: 4072 820 626 504 BbeI G_GCGC'C Cuts at: 2138 2138 Size: 6022 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2578 928 Size: 971 679 4372 Fragments arranged by size: 4372 971 679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4213 4247 1903 Size: 34 50 34 2192 34 3678 Fragments arranged by size: 3678 2192 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 3439 3439 Size: 6022 Bme1580I G_kGCm'C Cuts at: 1790 4083 5239 1790 Size: 2293 1156 2573 Fragments arranged by size: 2573 2293 1156 BmgBI CAC'GTC Cuts at: 1318 2908 1318 Size: 1590 4432 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3753 4711 442 Size: 416 53 83 399 24 2336 958 1753 Fragments arranged by size: 2336 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2835 2835 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2321 2509 2626 2877 4682 1239 Size: 1082 188 117 251 1805 2579 Fragments arranged by size: 2579 1805 1082 251 188 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2879 4147 5012 5310 2879 Size: 1268 865 298 3591 Fragments arranged by size: 3591 1268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2363 4685 882 Size: 617 864 2322 2219 Fragments arranged by size: 2322 2219 864 617 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 6016 6016 Size: 6022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2135 3829 4211 4846 Size: 53 83 186 936 602 1694 382 635 Cuts at: 4846 275 Size: 1451 Fragments arranged by size: 1694 1451 936 635 602 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4455 4853 5127 5274 5685 4455 Size: 398 274 147 411 4792 Fragments arranged by size: 4792 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2319 2349 1178 Size: 30 165 30 916 30 4851 Fragments arranged by size: 4851 916 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2102 2340 2668 2744 2777 2865 3071 Size: 913 3 238 328 76 33 88 206 Cuts at: 3071 3115 3245 1186 Size: 44 130 3963 Fragments arranged by size: 3963 913 328 238 206 130 88 76 44 33 3 BseYI C'CCAG_C Cuts at: 2138 2320 5225 5721 2138 Size: 182 2905 496 2439 Fragments arranged by size: 2905 2439 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 1163 Size: 573 345 5104 Fragments arranged by size: 5104 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2292 2424 3472 4233 5261 1540 Size: 275 186 291 132 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 291 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2724 4083 4168 5239 2724 Size: 1359 85 1071 3507 Fragments arranged by size: 3507 1359 1071 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 BsmBI CGTCTCn'nnnn_ Cuts at: 2443 2914 2443 Size: 471 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2715 2724 3341 4083 4168 5239 Size: 399 421 504 9 617 742 85 1071 Cuts at: 5239 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 617 504 421 399 85 9 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3911 5641 3911 Size: 1730 4292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2544 3377 844 Size: 1700 833 3489 Fragments arranged by size: 3489 1700 833 BsrBI CCG'CTC Cuts at: 1394 1823 2202 2412 3909 1394 Size: 429 379 210 1497 3507 Fragments arranged by size: 3507 1497 429 379 210 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4666 4853 1700 Size: 110 2856 187 2869 Fragments arranged by size: 2869 2856 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 2468 1925 Size: 543 5479 BssSI C'ACGA_G Cuts at: 4082 5094 4082 Size: 1012 5010 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 2308 1184 Size: 1124 4898 BstYI r'GATC_y Cuts at: 1972 2542 3144 3352 3719 4104 4121 5573 5659 Size: 570 602 208 367 385 17 1452 86 Cuts at: 5659 5671 6017 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 602 570 385 367 346 208 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2286 2500 513 Size: 552 1221 214 4035 Fragments arranged by size: 4035 1221 552 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5283 Fragments arranged by size: 5283 683 56 BtsI GCAGTG_nn' Cuts at: 3538 4332 4352 3538 Size: 794 20 5208 Fragments arranged by size: 5208 794 20 ClaI AT'CG_AT Cuts at: 1554 2790 1554 Size: 1236 4786 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5029 809 Size: 507 3713 1802 Fragments arranged by size: 3713 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2421 2503 Size: 54 1425 50 275 18 459 132 82 Cuts at: 2503 3469 4358 5987 8 Size: 966 889 1629 43 Fragments arranged by size: 1629 1425 966 889 459 275 132 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2289 2421 3469 1537 Size: 275 477 132 1048 4090 Fragments arranged by size: 4090 1048 477 275 132 EarI CTCTTCn'nnn_ Cuts at: 1353 3246 3952 1353 Size: 1893 706 3423 Fragments arranged by size: 3423 1893 706 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2509 2626 2877 3270 4085 4682 Size: 393 689 188 117 251 393 815 597 Cuts at: 4682 5469 5737 1239 Size: 787 268 1524 Fragments arranged by size: 1524 815 787 689 597 393 393 268 251 188 117 EcoO109I rG'GnC_Cy Cuts at: 3042 3172 3042 Size: 130 5892 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 EcoRV GAT'ATC Cuts at: 2149 2149 Size: 6022 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 2138 2553 2649 2939 3097 5169 1678 Size: 460 415 96 290 158 2072 2531 Fragments arranged by size: 2531 2072 460 415 290 158 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2597 2629 2828 Size: 32 135 32 467 32 526 32 199 Cuts at: 2828 2860 4663 4695 4737 4769 1373 Size: 32 1803 32 42 32 2626 Fragments arranged by size: 2626 1803 526 467 199 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KasI G'GCGC_C Cuts at: 2134 2134 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2964 3135 5136 5320 1305 Size: 1659 171 2001 184 2007 Fragments arranged by size: 2007 2001 1659 184 171 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4200 Fragments arranged by size: 4200 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3980 4339 4498 518 Size: 648 2814 359 159 2042 Fragments arranged by size: 2814 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2299 2481 2502 3438 4115 5263 5508 1606 Size: 693 182 21 936 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 693 677 245 182 21 NarI GG'CG_CC Cuts at: 2135 2135 Size: 6022 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5470 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 3380 4925 1132 Size: 97 2151 1545 2229 Fragments arranged by size: 2229 2151 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6022 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6022 PfoI T'CCnGG_A Cuts at: 2394 3147 2394 Size: 753 5269 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3042 3042 Size: 6022 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PstI C_TGCA'G Cuts at: 830 2127 2541 3374 830 Size: 1297 414 833 3478 Fragments arranged by size: 3478 1297 833 414 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 PvuII CAG'CTG Cuts at: 2299 2299 Size: 6022 SacII CC_GC'GG Cuts at: 2503 2503 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 2541 3374 2541 Size: 833 5189 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 2123 2537 3370 4505 5186 5377 826 Size: 219 1078 414 833 1135 681 191 1471 Fragments arranged by size: 1471 1135 1078 833 681 414 219 191 SfoI GGC'GCC Cuts at: 2136 2136 Size: 6022 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 3349 1872 Size: 1477 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2894 4126 5027 5289 820 Size: 197 926 951 1232 901 262 1553 Fragments arranged by size: 1553 1232 951 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 3177 3461 513 Size: 552 2112 284 3074 Fragments arranged by size: 3074 2112 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 4049 4066 4219 4404 4849 325 Size: 1255 747 1722 17 153 185 445 1498 Fragments arranged by size: 1722 1498 1255 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4268 5980 96 Size: 274 80 33 51 1443 2291 1712 138 Fragments arranged by size: 2291 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 2390 2422 3301 3333 5982 6014 2319 Size: 32 39 32 879 32 2649 32 2327 Fragments arranged by size: 2649 2327 879 39 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2686 2990 1316 Size: 1370 304 4348 Fragments arranged by size: 4348 1370 304 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 3347 1870 Size: 1477 4545 XmnI GAAnn'nnTTC Cuts at: 2159 4151 2159 Size: 1992 4030 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI