Restriction map for pFN21AE1082
 (Linear) (Six-base) MAPSORT of: KIEE1082.seq  Check: 2390  from: 1  to: 1317



KIEE1082.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

AclI AA'CG_TT

Cuts at:      0      11    1317 
   Size:         11    1306
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1265    1317 
   Size:       1265      52
 
AlwNI CAG_nnn'CTG

Cuts at:      0     509     540     835    1317 
   Size:        509      31     295     482

  Fragments arranged by size: 

                 509     482     295      31
 
ApoI r'AATT_y

Cuts at:      0     154    1317 
   Size:        154    1163
 
AvaI C'yCGr_G

Cuts at:      0     703    1317 
   Size:        703     614
 
BanI G'GyrC_C

Cuts at:      0     129     896    1317 
   Size:        129     767     421

  Fragments arranged by size: 

                 767     421     129
 
BanII G_rGCy'C

Cuts at:      0     206     710    1317 
   Size:        206     504     607

  Fragments arranged by size: 

                 607     504     206
 
BbeI G_GCGC'C

Cuts at:      0     133    1317 
   Size:        133    1184
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     573    1317 
   Size:        573     744
 
BmgBI CAC'GTC

Cuts at:      0     903    1317 
   Size:        903     414
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     830    1317 
   Size:        830     487
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     504     621     872    1317 
   Size:        316     188     117     251     445

  Fragments arranged by size: 

                 445     316     251     188     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874    1317 
   Size:        874     443
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     358    1317 
   Size:        358     959
 
BsaHI Gr'CG_yC

Cuts at:      0     130    1317 
   Size:        130    1187
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     314     344    1317 
   Size:        314      30     973

  Fragments arranged by size: 

                 973     314      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94      97     335     663     739     772     860    1066 
   Size:         94       3     238     328      76      33      88     206

Cuts at:   1066    1110    1240    1317 
   Size:         44     130      77

  Fragments arranged by size: 

                 328     238     206     130      94      88      77      76
                  44      33       3
 
BseYI C'CCAG_C

Cuts at:      0     133     315    1317 
   Size:        133     182    1002

  Fragments arranged by size: 

                1002     182     133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1317 
   Size:         76    1241
 
BsiEI CG_ry'CG

Cuts at:      0     287     419    1317 
   Size:        287     132     898

  Fragments arranged by size: 

                 898     287     132
 
BsiHKAI G_wGCw'C

Cuts at:      0     719    1317 
   Size:        719     598
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     438     909    1317 
   Size:        438     471     408

  Fragments arranged by size: 

                 471     438     408
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     710     719    1317 
   Size:        206     504       9     598

  Fragments arranged by size: 

                 598     504     206       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     539    1317 
   Size:        539     778
 
BsrBI CCG'CTC

Cuts at:      0     197     407    1317 
   Size:        197     210     910

  Fragments arranged by size: 

                 910     210     197
 
BssHII G'CGCG_C

Cuts at:      0     463    1317 
   Size:        463     854
 
BstEII G'GTnAC_C

Cuts at:      0     303    1317 
   Size:        303    1014
 
BstYI r'GATC_y

Cuts at:      0     537    1139    1317 
   Size:        537     602     178

  Fragments arranged by size: 

                 602     537     178
 
BtgI C'CryG_G

Cuts at:      0     281     495    1317 
   Size:        281     214     822

  Fragments arranged by size: 

                 822     281     214
 
ClaI AT'CG_AT

Cuts at:      0     785    1317 
   Size:        785     532
 
EaeI y'GGCC_r

Cuts at:      0     284     416     498    1317 
   Size:        284     132      82     819

  Fragments arranged by size: 

                 819     284     132      82
 
EagI C'GGCC_G

Cuts at:      0     284     416    1317 
   Size:        284     132     901

  Fragments arranged by size: 

                 901     284     132
 
EarI CTCTTCn'nnn_

Cuts at:      0    1241    1317 
   Size:       1241      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     504     621     872    1265    1317 
   Size:        316     188     117     251     393      52

  Fragments arranged by size: 

                 393     316     251     188     117      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1037    1167    1317 
   Size:       1037     130     150

  Fragments arranged by size: 

                1037     150     130
 
EcoRV GAT'ATC

Cuts at:      0     144    1317 
   Size:        144    1173
 
HaeII r_GCGC'y

Cuts at:      0     133     548     644     934    1092    1317 
   Size:        133     415      96     290     158     225

  Fragments arranged by size: 

                 415     290     225     158     133      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     592     624     823     855    1317 
   Size:         34      32     526      32     199      32     462

  Fragments arranged by size: 

                 526     462     199      34      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     129    1317 
   Size:        129    1188
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     959    1130    1317 
   Size:        959     171     187

  Fragments arranged by size: 

                 959     187     171
 
MspA1I CmG'CkG

Cuts at:      0     294     476     497    1317 
   Size:        294     182      21     820

  Fragments arranged by size: 

                 820     294     182      21
 
NarI GG'CG_CC

Cuts at:      0     130    1317 
   Size:        130    1187
 
PfoI T'CCnGG_A

Cuts at:      0     389    1142    1317 
   Size:        389     753     175

  Fragments arranged by size: 

                 753     389     175
 
PpuMI rG'GwC_Cy

Cuts at:      0    1037    1317 
   Size:       1037     280
 
PstI C_TGCA'G

Cuts at:      0     122     536    1317 
   Size:        122     414     781

  Fragments arranged by size: 

                 781     414     122
 
PvuII CAG'CTG

Cuts at:      0     294    1317 
   Size:        294    1023
 
SacII CC_GC'GG

Cuts at:      0     498    1317 
   Size:        498     819
 
SbfI CC_TGCA'GG

Cuts at:      0     536    1317 
   Size:        536     781
 
SfcI C'TryA_G

Cuts at:      0     118     532    1317 
   Size:        118     414     785

  Fragments arranged by size: 

                 785     414     118
 
SfoI GGC'GCC

Cuts at:      0     131    1317 
   Size:        131    1186
 
SmlI C'TyrA_G

Cuts at:      0     889    1317 
   Size:        889     428
 
StyI C'CwwG_G

Cuts at:      0    1172    1317 
   Size:       1172     145
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     322    1317 
   Size:        322     995
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     314     346     385     417    1296    1317 
   Size:        314      32      39      32     879      21

  Fragments arranged by size: 

                 879     314      39      32      32      21
 
Tth111I GACn'n_nGTC

Cuts at:      0     681     985    1317 
   Size:        681     304     332

  Fragments arranged by size: 

                 681     332     304
 
XmnI GAAnn'nnTTC

Cuts at:      0     154    1317 
   Size:        154    1163
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI   BssHII   BstEII    BstYI     BtgI     ClaI     EaeI     EagI
     EarI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     KasI     MmeI
   MspA1I     NarI     PfoI    PpuMI     PstI    PvuII    SacII     SbfI
     SfcI     SfoI     SmlI     StyI    TaqII     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI   Bpu10I
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     XbaI
     XcmI     XhoI     XmaI     ZraI
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