Restriction map for pFN21AB7932
 (Circular) (Six-base) MAPSORT of: pFN21AB7932.seq  Check: 6175  from: 1  to: 5344



pFN21AB7932  5344 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 11:00  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3154     278 
   Size:         53      83     186     936    1618    2468

  Fragments arranged by size: 

                2468    1618     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2471    2687    1937 
   Size:        534     216    4594

  Fragments arranged by size: 

                4594     534     216
 
Acc65I G'GTAC_C

Cuts at:   2665    2665 
   Size:       5344
 
AclI AA'CG_TT

Cuts at:   1563    3471    3844    1563 
   Size:       1908     373    3063

  Fragments arranged by size: 

                3063    1908     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2100    2344    3407    4791    5059    1632 
   Size:        468     244    1063    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1063     468     268     244
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5147
 
AflIII A'CryG_T

Cuts at:   1203    4243    5220    1203 
   Size:       3040     977    1327

  Fragments arranged by size: 

                3040    1327     977
 
AgeI A'CCGG_T

Cuts at:   4175    4175 
   Size:       5344
 
AhdI GACnn_n'nnGTC

Cuts at:   4073    4073 
   Size:       5344
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5344
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5344
 
AlwNI CAG_nnn'CTG

Cuts at:   3087    4659    3087 
   Size:       1572    3772
 
ApaLI G'TGCA_C

Cuts at:   3401    4557    3401 
   Size:       1156    4188
 
ApoI r'AATT_y

Cuts at:   1493    2653    2879    3031    5140    1493 
   Size:       1160     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1160     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3898     160 
   Size:        872    2866    1606

  Fragments arranged by size: 

                2866    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5344
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2669    1870 
   Size:         73     726    4545

  Fragments arranged by size: 

                4545     726      73
 
BamHI G'GATC_C

Cuts at:   2674    3041    2674 
   Size:        367    4977
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2105    2150    2665    4120     618 
   Size:        325     217     627     318      45     515    1455    1842

  Fragments arranged by size: 

                1842    1455     627     515     325     318     217      45
            
 
BanII G_rGCy'C

Cuts at:   1391    2217    2663    1391 
   Size:        826     446    4072

  Fragments arranged by size: 

                4072     826     446
 
BbeI G_GCGC'C

Cuts at:   2109    2154    2109 
   Size:         45    5299
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2091    2577     928 
   Size:        971     192     486    3695

  Fragments arranged by size: 

                3695     971     486     192
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3535    3569    1903 
   Size:         34      50      34    1514      34    3678

  Fragments arranged by size: 

                3678    1514      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3236    4446    3236 
   Size:       1210    4134
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5344
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2266    3955     136 
   Size:        107     122      71     631     605     594    1689    1525

  Fragments arranged by size: 

                1689    1525     631     605     594     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2563    5339    2563 
   Size:       2776    2568
 
BlpI GC'TnA_GC

Cuts at:   2761    2761 
   Size:       5344
 
Bme1580I G_kGCm'C

Cuts at:   1790    3405    4561    1790 
   Size:       1615    1156    2573

  Fragments arranged by size: 

                2573    1615    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5344
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2035    2156    3075 
   Size:        416      53      83     399      24     618     121     919

Cuts at:   3075    4033     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     958     919     618     416     399     121      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2477    2618    4004    1239 
   Size:       1238     141    1386    2579

  Fragments arranged by size: 

                2579    1386    1238     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3469    4334    4632    3469 
   Size:        865     298    4181

  Fragments arranged by size: 

                4181     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4007     882 
   Size:        617    2508    2219

  Fragments arranged by size: 

                2508    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3135     493 
   Size:        711    1931    2702

  Fragments arranged by size: 

                2702    1931     711
 
BsaBI GATnn'nnATC

Cuts at:   5338    5338 
   Size:       5344
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2061    2106    2151    3151 
   Size:         53      83     186     936     528      45      45    1000

Cuts at:   3151    3533    4168     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                1451    1000     936     635     528     382     186      83
                  53      45      45
 
BsaWI w'CCGG_w

Cuts at:   2502    3777    4175    4449    4596    5007    2502 
   Size:       1275     398     274     147     411    2839

  Fragments arranged by size: 

                2839    1275     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5119

  Fragments arranged by size: 

                5119     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2066    2111    2126    2171    2174    1186 
   Size:        880      45      15      45       3    4356

  Fragments arranged by size: 

                4356     880      45      45      15       3
 
BseYI C'CCAG_C

Cuts at:   4547    5043    4547 
   Size:        496    4848
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2606    1163 
   Size:        573     870    3901

  Fragments arranged by size: 

                3901     870     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2180    2794    3555    4583    1540 
   Size:        275     186     179     614     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     614     275     186     179
 
BsiHKAI G_wGCw'C

Cuts at:   3405    3490    4561    3405 
   Size:         85    1071    4188

  Fragments arranged by size: 

                4188    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2855    2948    2855 
   Size:         93    5251
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2217    2663    3405    3490    4561    1391 
   Size:        399     427     446     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     446     427     399      85
 
BspEI T'CCGG_A

Cuts at:   2502    5007    2502 
   Size:       2505    2839
 
BspHI T'CATG_A

Cuts at:   3233    4963    3233 
   Size:       1730    3614
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2699     844 
   Size:       1855    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2602    3231    1394 
   Size:        429     779     629    3507

  Fragments arranged by size: 

                3507     779     629     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2619    3839    4013      65 
   Size:       1183    1371    1220     174    1396

  Fragments arranged by size: 

                1396    1371    1220    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3988    4175    1700 
   Size:        110    2178     187    2869

  Fragments arranged by size: 

                2869    2178     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5344
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5344
 
BssSI C'ACGA_G

Cuts at:   3404    4416    3404 
   Size:       1012    4332
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5344
 
BstBI TT'CG_AA

Cuts at:   4148    4148 
   Size:       5344
 
BstEII G'GTnAC_C

Cuts at:   1184    2345    1184 
   Size:       1161    4183
 
BstYI r'GATC_y

Cuts at:   1972    2563    2674    3041    3426    3443    4895    4981    4993 
   Size:        591     111     367     385      17    1452      86      12

Cuts at:   4993    5339    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     591     385     367     346     111      86
                  17      12
 
BstZ17I GTA'TAC

Cuts at:   2472    2472 
   Size:       5344
 
BtgI C'CryG_G

Cuts at:    513    1065    2180     513 
   Size:        552    1115    3677

  Fragments arranged by size: 

                3677    1115     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2500     487 
   Size:        683      56    1274    3331

  Fragments arranged by size: 

                3331    1274     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2459    2860    3654    3674    2459 
   Size:        401     794      20    4129

  Fragments arranged by size: 

                4129     794     401      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5344
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5309
 
DraI TTT'AAA

Cuts at:   2648    2648 
   Size:       5344
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4351     809 
   Size:        507    3035    1802

  Fragments arranged by size: 

                3035    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2177    2791    3680 
   Size:         54    1425      50     275      18     347     614     889

Cuts at:   3680    5309       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     614     347     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2177    2791    1537 
   Size:        275     365     614    4090

  Fragments arranged by size: 

                4090     614     365     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2061    2121    2363    3274    1353 
   Size:        708      60     242     911    3423

  Fragments arranged by size: 

                3423     911     708     242      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2033    3931    4305    4451     387 
   Size:       1064     138     444    1898     374     146    1280

  Fragments arranged by size: 

                1898    1280    1064     444     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2100    2344    2477    2618    3407    4004    4791 
   Size:        393     468     244     133     141     789     597     787

Cuts at:   4791    5059    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     789     787     597     468     393     268     244
                 141     133
 
EcoRI G'AATT_C

Cuts at:   2653    3031    2653 
   Size:        378    4966
 
FspI TGC'GCA

Cuts at:   3850    3850 
   Size:       5344
 
HaeII r_GCGC'y

Cuts at:   1678    2109    2154    4491    1678 
   Size:        431      45    2337    2531

  Fragments arranged by size: 

                2531    2337     431      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2526    2558    3985    4017    4059 
   Size:         32     135      32     954      32    1427      32      42

Cuts at:   4059    4091    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1427     954     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2395    2431    2688    2934     669 
   Size:        988     281     457      36     257     246    3079

  Fragments arranged by size: 

                3079     988     457     281     257     246      36
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5344
 
HpaI GTT'AAC

Cuts at:   2395    2934    2395 
   Size:        539    4805
 
KasI G'GCGC_C

Cuts at:   2105    2150    2105 
   Size:         45    5299
 
KpnI G_GTAC'C

Cuts at:   2669    2669 
   Size:       5344
 
MfeI C'AATT_G

Cuts at:   2943    2943 
   Size:       5344
 
MluI A'CGCG_T

Cuts at:   5220    5220 
   Size:       5344
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4458    4642    1305 
   Size:       3153     184    2007

  Fragments arranged by size: 

                3153    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3522

  Fragments arranged by size: 

                3522    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2580    3302    3661    3820     518 
   Size:        648    1414     722     359     159    2042

  Fragments arranged by size: 

                2042    1414     722     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2182    2760    3437    4585    4830    1606 
   Size:        576     578     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     578     576     245
 
NarI GG'CG_CC

Cuts at:   2106    2151    2106 
   Size:         45    5299
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4792
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5344
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5344
 
NotI GC'GGCC_GC

Cuts at:   2177    2791    2177 
   Size:        614    4730
 
NspI r_CATG'y

Cuts at:   1132    1229    2702    4247    1132 
   Size:         97    1473    1545    2229

  Fragments arranged by size: 

                2229    1545    1473      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5344
 
PciI A'CATG_T

Cuts at:   4243    4243 
   Size:       5344
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5344
 
PmeI GTTT'AAAC

Cuts at:   2648    2648 
   Size:       5344
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3413    3445    4926    4958    3413 
   Size:         32    1481      32    3799

  Fragments arranged by size: 

                3799    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5344
 
PsiI TTA'TAA

Cuts at:   2914    2914 
   Size:       5344
 
PstI C_TGCA'G

Cuts at:    830    2696     830 
   Size:       1866    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5344
 
SacII CC_GC'GG

Cuts at:   2183    2183 
   Size:       5344
 
SalI G'TCGA_C

Cuts at:   1936    2686    1936 
   Size:        750    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2363    2363 
   Size:       5344
 
SbfI CC_TGCA'GG

Cuts at:   2696    2696 
   Size:       5344
 
ScaI AGT'ACT

Cuts at:   3592    5304    3592 
   Size:       1712    3632
 
SfcI C'TryA_G

Cuts at:    826    1045    2692    3827    4508    4699     826 
   Size:        219    1647    1135     681     191    1471

  Fragments arranged by size: 

                1647    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2107    2152    2107 
   Size:         45    5299
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5344
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5344
 
SmaI CCC'GGG

Cuts at:   1872    2671    1872 
   Size:        799    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3448    4349    4611     820 
   Size:        197     926    1505     901     262    1553

  Fragments arranged by size: 

                1553    1505     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3135     493 
   Size:       2642    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5344
 
SphI G_CATG'C

Cuts at:   1132    2702    1132 
   Size:       1570    3774
 
SspI AAT'ATT

Cuts at:      5      52    3268       5 
   Size:         47    3216    2081

  Fragments arranged by size: 

                3216    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5344
 
StyI C'CwwG_G

Cuts at:    513    1065    2783     513 
   Size:        552    1718    3074

  Fragments arranged by size: 

                3074    1718     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2592    3371    3388    3541    3726    4171     325 
   Size:       1255    1012     779      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1012     779     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3590    5302      96 
   Size:        274      80      33      51    1443    1613    1712     138

  Fragments arranged by size: 

                1712    1613    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5304    5336    5304 
   Size:         32    5312
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2543    1316 
   Size:       1227    4117
 
XbaI T'CTAG_A

Cuts at:   2680    2680 
   Size:       5344
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5344
 
XmaI C'CCGG_G

Cuts at:   1870    2669    1870 
   Size:        799    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3473    3473 
   Size:       5344
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3152     276 
   Size:         53      83     186     936    1618    2468

  Fragments arranged by size: 

                2468    1618     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    SacII
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     NaeI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SanDI    SexAI     SfiI
     SrfI     SwaI     XcmI
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