(Circular) (Six-base) MAPSORT of: pFN21AB6271.seq Check: 6911 from: 1 to: 6715 pFN21AB6271 6715 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2896 3684 3751 2896 Size: 788 67 5860 Fragments arranged by size: 5860 788 67 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4525 278 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2372 4058 1937 Size: 435 1686 4594 Fragments arranged by size: 4594 1686 435 Acc65I G'GTAC_C Cuts at: 4036 4036 Size: 6715 AclI AA'CG_TT Cuts at: 1563 4842 5215 1563 Size: 3279 373 3063 Fragments arranged by size: 3279 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2366 2402 2746 3254 3671 3749 4778 Size: 644 90 36 344 508 417 78 1029 Cuts at: 4778 6162 6430 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1029 644 508 417 344 268 90 78 36 AfeI AGC'GCT Cuts at: 2812 3341 2812 Size: 529 6186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6518 AflIII A'CryG_T Cuts at: 1203 5614 6591 1203 Size: 4411 977 1327 Fragments arranged by size: 4411 1327 977 AgeI A'CCGG_T Cuts at: 3522 5546 3522 Size: 2024 4691 AhdI GACnn_n'nnGTC Cuts at: 5444 5444 Size: 6715 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6715 AlwNI CAG_nnn'CTG Cuts at: 2254 3199 3334 4458 6030 2254 Size: 945 135 1124 1572 2939 Fragments arranged by size: 2939 1572 1124 945 135 ApaI G_GGCC'C Cuts at: 2119 3498 3543 3830 2119 Size: 1379 45 287 5004 Fragments arranged by size: 5004 1379 287 45 ApaLI G'TGCA_C Cuts at: 2399 2972 4772 5928 2399 Size: 573 1800 1156 3186 Fragments arranged by size: 3186 1800 1156 573 ApoI r'AATT_y Cuts at: 1493 4024 4250 4402 6511 1493 Size: 2531 226 152 2109 1697 Fragments arranged by size: 2531 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2690 2690 Size: 6715 AseI AT'TA_AT Cuts at: 160 1032 5269 160 Size: 872 4237 1606 Fragments arranged by size: 4237 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 AvaI C'yCGr_G Cuts at: 1870 1943 2570 3174 3555 4040 1870 Size: 73 627 604 381 485 4545 Fragments arranged by size: 4545 627 604 485 381 73 BamHI G'GATC_C Cuts at: 4045 4412 4045 Size: 367 6348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2417 2666 2717 3749 Size: 325 217 627 249 381 249 51 1032 Cuts at: 3749 4036 5491 618 Size: 287 1455 1842 Fragments arranged by size: 1842 1455 1032 627 381 325 287 249 249 217 51 BanII G_rGCy'C Cuts at: 1391 2054 2119 2277 2577 2801 3126 3366 3498 Size: 663 65 158 300 224 325 240 132 Cuts at: 3498 3543 3830 4034 1391 Size: 45 287 204 4072 Fragments arranged by size: 4072 663 325 300 287 240 224 204 158 132 65 45 BbeI G_GCGC'C Cuts at: 2040 2421 3753 2040 Size: 381 1332 5002 Fragments arranged by size: 5002 1332 381 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3136 928 Size: 971 1237 4507 Fragments arranged by size: 4507 1237 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4906 4940 1903 Size: 34 2969 34 3678 Fragments arranged by size: 3678 2969 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4607 5817 4607 Size: 1210 5505 BclI T'GATC_A Cuts at: 1548 1548 Size: 6715 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2756 2897 2951 Size: 107 122 71 631 605 1084 141 54 Cuts at: 2951 3641 5326 136 Size: 690 1685 1525 Fragments arranged by size: 1685 1525 1084 690 631 605 141 122 107 71 54 BglII A'GATC_T Cuts at: 6710 6710 Size: 6715 BlpI GC'TnA_GC Cuts at: 2225 2276 4132 2225 Size: 51 1856 4808 Fragments arranged by size: 4808 1856 51 Bme1580I G_kGCm'C Cuts at: 1790 2119 2403 2629 2671 2722 2976 3498 3543 Size: 329 284 226 42 51 254 522 45 Cuts at: 3543 3830 3967 4776 5932 1790 Size: 287 137 809 1156 2573 Fragments arranged by size: 2573 1156 809 522 329 287 284 254 226 137 51 45 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6715 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2613 3962 4446 Size: 416 53 83 399 24 1196 1349 484 Cuts at: 4446 5404 442 Size: 958 1753 Fragments arranged by size: 1753 1349 1196 958 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6715 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2800 2896 2410 Size: 390 96 6229 Fragments arranged by size: 6229 390 96 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2240 2288 2468 2519 2852 3221 3934 Size: 794 207 48 180 51 333 369 713 Cuts at: 3934 3963 5375 1239 Size: 29 1412 2579 Fragments arranged by size: 2579 1412 794 713 369 333 207 180 51 48 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4840 5705 6003 4840 Size: 865 298 5552 Fragments arranged by size: 5552 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2345 5378 882 Size: 617 846 3033 2219 Fragments arranged by size: 3033 2219 846 617 BsaAI yAC'GTr Cuts at: 493 1204 3010 3079 3885 4506 493 Size: 711 1806 69 806 621 2702 Fragments arranged by size: 2702 1806 806 711 621 69 BsaBI GATnn'nnATC Cuts at: 6709 6709 Size: 6715 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2418 3750 4522 Size: 53 83 186 936 504 381 1332 772 Cuts at: 4522 4904 5539 275 Size: 382 635 1451 Fragments arranged by size: 1451 1332 936 772 635 504 382 381 186 83 53 BsaWI w'CCGG_w Cuts at: 2976 3522 5148 5546 5820 5967 6378 2976 Size: 546 1626 398 274 147 411 3313 Fragments arranged by size: 3313 1626 546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 2405 2435 3044 Size: 30 165 30 819 30 153 30 609 Cuts at: 3044 3074 1178 Size: 30 4819 Fragments arranged by size: 4819 819 609 165 153 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2060 2276 2285 3154 3218 3272 3573 Size: 844 30 216 9 869 64 54 301 Cuts at: 3573 3896 1186 Size: 323 4005 Fragments arranged by size: 4005 869 844 323 301 216 64 54 30 9 BseYI C'CCAG_C Cuts at: 2477 2616 2753 2919 2988 3106 3490 3779 3794 Size: 139 137 166 69 118 384 289 15 Cuts at: 3794 3823 3927 3954 5918 6414 2477 Size: 29 104 27 1964 496 2778 Fragments arranged by size: 2778 1964 496 384 289 166 139 137 118 104 69 29 27 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2692 3257 1163 Size: 573 956 565 4621 Fragments arranged by size: 4621 956 573 565 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2214 3114 3370 4165 4926 5954 Size: 275 186 213 900 256 795 761 1028 Cuts at: 5954 1540 Size: 2301 Fragments arranged by size: 2301 1028 900 795 761 275 256 213 186 BsiHKAI G_wGCw'C Cuts at: 2277 2403 2976 3126 3366 4776 4861 5932 2277 Size: 126 573 150 240 1410 85 1071 3060 Fragments arranged by size: 3060 1410 1071 573 240 150 126 85 BsmI GAATG_Cn' Cuts at: 4226 4319 4226 Size: 93 6622 BsmBI CGTCTCn'nnnn_ Cuts at: 2201 2201 Size: 6715 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2119 2277 2403 2577 2629 2671 Size: 399 264 65 158 126 174 52 42 Cuts at: 2671 2722 2801 2976 3126 3366 3498 3543 3830 Size: 51 79 175 150 240 132 45 287 Cuts at: 3830 3967 4034 4776 4861 5932 1391 Size: 137 67 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 287 264 240 175 174 158 150 137 132 126 85 79 67 65 52 51 45 42 BspEI T'CCGG_A Cuts at: 6378 6378 Size: 6715 BspHI T'CATG_A Cuts at: 2841 4604 6334 2841 Size: 1763 1730 3222 Fragments arranged by size: 3222 1763 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2253 2896 3130 3315 3684 3751 4070 844 Size: 1409 643 234 185 369 67 319 3489 Fragments arranged by size: 3489 1409 643 369 319 234 185 67 BsrBI CCG'CTC Cuts at: 1394 1823 2703 3060 4602 1394 Size: 429 880 357 1542 3507 Fragments arranged by size: 3507 1542 880 429 357 BsrDI GCAATG_nn' Cuts at: 65 1248 5210 5384 65 Size: 1183 3962 174 1396 Fragments arranged by size: 3962 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 2298 3522 3810 4012 5359 5546 Size: 110 229 259 1224 288 202 1347 187 Cuts at: 5546 1700 Size: 2869 Fragments arranged by size: 2869 1347 1224 288 259 229 202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6715 BssHII G'CGCG_C Cuts at: 1925 2543 2690 1925 Size: 618 147 5950 Fragments arranged by size: 5950 618 147 BssSI C'ACGA_G Cuts at: 3134 4775 5787 3134 Size: 1641 1012 4062 Fragments arranged by size: 4062 1641 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2467 794 Size: 1673 5042 BstBI TT'CG_AA Cuts at: 5519 5519 Size: 6715 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6715 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2362 3696 2011 Size: 351 1334 5030 Fragments arranged by size: 5030 1334 351 BstYI r'GATC_y Cuts at: 1972 2085 4045 4412 4797 4814 6266 6352 6364 Size: 113 1960 367 385 17 1452 86 12 Cuts at: 6364 6710 1972 Size: 346 1977 Fragments arranged by size: 1977 1960 1452 385 367 346 113 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2771 2771 Size: 6715 BtgI C'CryG_G Cuts at: 513 1065 2054 2895 2982 3084 3636 513 Size: 552 989 841 87 102 552 3592 Fragments arranged by size: 3592 989 841 552 552 102 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3592 487 Size: 683 56 2366 3610 Fragments arranged by size: 3610 2366 683 56 BtsI GCAGTG_nn' Cuts at: 4231 5025 5045 4231 Size: 794 20 5901 Fragments arranged by size: 5901 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6715 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6680 DraI TTT'AAA Cuts at: 4019 4019 Size: 6715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3599 5722 809 Size: 507 2283 2123 1802 Fragments arranged by size: 2283 2123 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2296 3348 Size: 54 1425 50 275 18 265 201 1052 Cuts at: 3348 4010 4162 5051 6680 8 Size: 662 152 889 1629 43 Fragments arranged by size: 1629 1425 1052 889 662 275 265 201 152 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4162 1537 Size: 275 2350 4090 Fragments arranged by size: 4090 2350 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2582 2660 4645 1353 Size: 1229 78 1985 3423 Fragments arranged by size: 3423 1985 1229 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3972 5302 5676 5822 387 Size: 1064 138 2383 1330 374 146 1280 Fragments arranged by size: 2383 1330 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 3124 3364 2275 Size: 849 240 5626 Fragments arranged by size: 5626 849 240 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2240 2276 2288 2366 2402 2468 Size: 393 401 207 36 12 78 36 66 Cuts at: 2468 2519 2746 2852 3221 3254 3671 3749 3934 Size: 51 227 106 369 33 417 78 185 Cuts at: 3934 3963 4778 5375 6162 6430 1239 Size: 29 815 597 787 268 1524 Fragments arranged by size: 1524 815 787 597 417 401 393 369 268 227 207 185 106 78 78 66 51 36 36 33 29 12 EcoNI CCTnn'n_nnAGG Cuts at: 2459 2743 2933 3251 3296 3458 3653 2459 Size: 284 190 318 45 162 195 5521 Fragments arranged by size: 5521 318 284 195 190 162 45 EcoO109I rG'GnC_Cy Cuts at: 2115 2452 2608 2757 2875 3179 3400 3470 3494 Size: 337 156 149 118 304 221 70 24 Cuts at: 3494 3495 3539 3827 2115 Size: 1 44 288 5003 Fragments arranged by size: 5003 337 304 288 221 156 149 118 70 44 24 1 EcoRI G'AATT_C Cuts at: 4024 4402 4024 Size: 378 6337 FspI TGC'GCA Cuts at: 2827 5221 2827 Size: 2394 4321 HaeII r_GCGC'y Cuts at: 1678 2040 2166 2421 2814 3266 3343 3753 5862 Size: 362 126 255 393 452 77 410 2109 Cuts at: 5862 1678 Size: 2531 Fragments arranged by size: 2531 2109 452 410 393 362 255 126 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2196 2228 2405 2437 5356 Size: 32 135 32 624 32 177 32 2919 Cuts at: 5356 5388 5430 5462 1373 Size: 32 42 32 2626 Fragments arranged by size: 2919 2626 624 177 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 4059 4305 669 Size: 988 281 298 1823 246 3079 Fragments arranged by size: 3079 1823 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6715 HpaI GTT'AAC Cuts at: 4305 4305 Size: 6715 KasI G'GCGC_C Cuts at: 2036 2417 3749 2036 Size: 381 1332 5002 Fragments arranged by size: 5002 1332 381 KpnI G_GTAC'C Cuts at: 4040 4040 Size: 6715 MfeI C'AATT_G Cuts at: 4314 4314 Size: 6715 MluI A'CGCG_T Cuts at: 6591 6591 Size: 6715 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5829 6013 1305 Size: 960 3564 184 2007 Fragments arranged by size: 3564 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3350 10 Size: 54 1425 343 1518 3375 Fragments arranged by size: 3375 1518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3694 3883 4673 5032 5191 518 Size: 648 2528 189 790 359 159 2042 Fragments arranged by size: 2528 2042 790 648 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2056 2101 2251 2280 2380 2527 2753 2923 Size: 450 45 150 29 100 147 226 170 Cuts at: 2923 3638 4131 4808 5956 6201 1606 Size: 715 493 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 493 450 245 226 170 150 147 100 45 29 NaeI GCC'GGC Cuts at: 2041 2300 3812 2041 Size: 259 1512 4944 Fragments arranged by size: 4944 1512 259 NarI GG'CG_CC Cuts at: 2037 2418 3750 2037 Size: 381 1332 5002 Fragments arranged by size: 5002 1332 381 NcoI C'CATG_G Cuts at: 513 1065 2895 2982 3084 513 Size: 552 1830 87 102 4144 Fragments arranged by size: 4144 1830 552 102 87 NdeI CA'TA_TG Cuts at: 387 387 Size: 6715 NgoMIV G'CCGG_C Cuts at: 2039 2298 3810 2039 Size: 259 1512 4944 Fragments arranged by size: 4944 1512 259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6715 NotI GC'GGCC_GC Cuts at: 4162 4162 Size: 6715 NspI r_CATG'y Cuts at: 1132 1229 3041 4073 5618 1132 Size: 97 1812 1032 1545 2229 Fragments arranged by size: 2229 1812 1545 1032 97 PasI CC'CwG_GG Cuts at: 1348 2338 3460 3831 4004 1348 Size: 990 1122 371 173 4059 Fragments arranged by size: 4059 1122 990 371 173 PciI A'CATG_T Cuts at: 5614 5614 Size: 6715 PflMI CCAn_nnn'nTGG Cuts at: 1108 3490 1108 Size: 2382 4333 PfoI T'CCnGG_A Cuts at: 2010 2535 3020 2010 Size: 525 485 5705 Fragments arranged by size: 5705 525 485 PmeI GTTT'AAAC Cuts at: 4019 4019 Size: 6715 PmlI CAC'GTG Cuts at: 3885 3885 Size: 6715 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4784 4816 6297 6329 4784 Size: 32 1481 32 5170 Fragments arranged by size: 5170 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 2608 Size: 6715 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6715 PsiI TTA'TAA Cuts at: 4285 4285 Size: 6715 PspOMI G'GGCC_C Cuts at: 2115 3494 3539 3826 2115 Size: 1379 45 287 5004 Fragments arranged by size: 5004 1379 287 45 PstI C_TGCA'G Cuts at: 830 2064 2106 2151 2250 3153 3339 3762 4067 Size: 1234 42 45 99 903 186 423 305 Cuts at: 4067 830 Size: 3478 Fragments arranged by size: 3478 1234 903 423 305 186 99 45 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6715 PvuII CAG'CTG Cuts at: 2101 2251 2527 2923 2101 Size: 150 276 396 5893 Fragments arranged by size: 5893 396 276 150 SacI G_AGCT'C Cuts at: 2277 3126 3366 2277 Size: 849 240 5626 Fragments arranged by size: 5626 849 240 SacII CC_GC'GG Cuts at: 2057 3639 2057 Size: 1582 5133 SalI G'TCGA_C Cuts at: 1936 4057 1936 Size: 2121 4594 SanDI GG'GwC_CC Cuts at: 2608 2608 Size: 6715 SapI GCTCTTCn'nnn_ Cuts at: 2582 2582 Size: 6715 SbfI CC_TGCA'GG Cuts at: 3762 4067 3762 Size: 305 6410 ScaI AGT'ACT Cuts at: 3053 4963 6675 3053 Size: 1910 1712 3093 Fragments arranged by size: 3093 1910 1712 SfcI C'TryA_G Cuts at: 826 1045 2060 2102 2147 2176 2246 3149 3335 Size: 219 1015 42 45 29 70 903 186 Cuts at: 3335 3758 4063 5198 5879 6070 826 Size: 423 305 1135 681 191 1471 Fragments arranged by size: 1471 1135 1015 903 681 423 305 219 191 186 70 45 42 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2756 2756 Size: 6715 SfoI GGC'GCC Cuts at: 2038 2419 3751 2038 Size: 381 1332 5002 Fragments arranged by size: 5002 1332 381 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 SgrAI Cr'CCGG_yG Cuts at: 1700 2039 1700 Size: 339 6376 SmaI CCC'GGG Cuts at: 1872 4042 1872 Size: 2170 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4819 5720 5982 820 Size: 197 926 2876 901 262 1553 Fragments arranged by size: 2876 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4506 493 Size: 4013 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6715 SphI G_CATG'C Cuts at: 1132 4073 1132 Size: 2941 3774 SspI AAT'ATT Cuts at: 5 52 4639 5 Size: 47 4587 2081 Fragments arranged by size: 4587 2081 47 StuI AGG'CCT Cuts at: 1517 2738 1517 Size: 1221 5494 StyI C'CwwG_G Cuts at: 513 1065 2355 2895 2982 3084 4154 513 Size: 552 1290 540 87 102 1070 3074 Fragments arranged by size: 3074 1290 1070 552 540 102 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3128 4017 4742 4759 4912 5097 5542 Size: 1255 1548 889 725 17 153 185 445 Cuts at: 5542 325 Size: 1498 Fragments arranged by size: 1548 1498 1255 889 725 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2961 3051 4961 Size: 274 80 33 51 1443 984 90 1910 Cuts at: 4961 6673 96 Size: 1712 138 Fragments arranged by size: 1910 1712 1443 984 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6675 6707 6675 Size: 32 6683 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6715 XbaI T'CTAG_A Cuts at: 3785 4051 3785 Size: 266 6449 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2335 2681 2750 3696 3760 3857 2335 Size: 346 69 946 64 97 5193 Fragments arranged by size: 5193 946 346 97 69 64 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6715 XmaI C'CCGG_G Cuts at: 1870 4040 1870 Size: 2170 4545 XmnI GAAnn'nnTTC Cuts at: 4844 4844 Size: 6715 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4523 276 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI SrfI SwaI