Restriction map for
pFN21AE0965
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HapII HgaI Hin1I
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tsp45I
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 193 GTCTAC GT^[AC][GT]AC
AflIII 98 ACATGT A^C[GA][CT]GT
Aor51HI 1136 AGCGCT AGC^GCT
Asp700I 702 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1089 GAGCAC G[AT]GC[AT]^C
AvaI 476 CCCGAG C^[CT]CG[GA]G
BanI 1129 GGCACC G^G[CT][GA]CC
BanII 505 GGGCTC G[GA]GC[CT]^C
BbsI 584 GTCTTC GAAGAC(2/6)
BbvCI 768 GCTGAGG CCTCAGC(-5/-2)
BciVI 900 GGATAC GTATCC(6/5)
BglII 704 AGATCT A^GATCT
BpmI 822 CTCCAG CTGGAG(16/14)
BpuAI 584 GTCTTC GAAGAC(2/6)
BsaI 115 GGTCTC GGTCTC(1/5)
BseRI 711 CTCCTC GAGGAG(10/8)
BsiHKAI 1089 GAGCAC G[AT]GC[AT]^C
BsmBI 479 GAGACG CGTCTC(1/5)
BsoBI 476 CCCGAG C^[CT]CG[GA]G
BspLU11I 98 ACATGT A^CATGT
BspMI 650 GCAGGT ACCTGC(4/8)
BstBI 917 TTCGAA TT^CGAA
BstYI 704 AGATCT [GA]^GATC[CT]
DraII 973 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 246 GACCCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 269 CCGTGG C^C[GA][CT]GG
Eco47III 1136 AGCGCT AGC^GCT
EcoO109I 973 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1078 ATGCAT ATGCA^T
FauI 249 CCCGC CCCGC(4/6)
HaeII 1136 AGCGCT [GA]GCGC^[CT]
HincII 82 GTCAAC GT[CT]^[GA]AC
HindII 82 GTCAAC GT[CT]^[GA]AC
MaeIII 296 GTAAC ^GT[ACGT]AC
MflI 704 AGATCT [GA]^GATC[CT]
NsiI 1078 ATGCAT ATGCA^T
NspI 98 ACATGT [GA]CATG^[CT]
NspV 917 TTCGAA TT^CGAA
PleI 1454 GACTC GAGTC(4/5)
PpuMI 973 GGGACCC [GA]G^G[AT]CC[CT]
SfcI 389 CTGTAG C^T[GA][CT]AG
SfuI 917 TTCGAA TT^CGAA
SspI 1021 AATATT AAT^ATT
XbaI 1158 TCTAGA T^CTAGA
XhoII 704 AGATCT [GA]^GATC[CT]
XmnI 702 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 354 GAATTT [GA]^AATT[CT]
442 AAATTT
ApoI 354 GAATTT [GA]^AATT[CT]
442 AAATTT
AvaII 135 GGACC G^G[AT]CC
974 GGACC
BsmI 1426 GAATGC GAATGC(1/-1)
1080 GCATTC
Bsp1407I 562 TGTACA T^GTACA
752 TGTACA
BspHI 35 TCATGA T^CATGA
855 TCATGA
BsrGI 562 TGTACA T^GTACA
752 TGTACA
BsrI 290 ACTGG ACTGG(1/-1)
824 CCAGT
BstXI 1169 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1244 CCAGGCGGATGG
CfoI 453 GCGC GCG^C
1137 GCGC
DraI 445 TTTAAA TTT^AAA
1008 TTTAAA
HhaI 453 GCGC GCG^C
1137 GCGC
HinP1I 453 GCGC G^CGC
1137 GCGC
MaeII 191 ACGT A^CGT
482 ACGT
MwoI 149 GCCAGCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1130 GCACCAAGCGC
PflMI 264 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1373 CCAACAGATGG
RcaI 35 TCATGA T^CATGA
855 TCATGA
SfaNI 765 GATGC GCATC(5/9)
173 GCATC
SspBI 562 TGTACA T^GTACA
752 TGTACA
TfiI 18 GATTC G^A[AT]TC
1189 GATTC
Van91I 264 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1373 CCAACAGATGG
XcmI 360 CCACTTGGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1133 CCAAGCGCTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MvaI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I