(Circular) (Six-base) MAPSORT of: pFN21AE0965.seq Check: 7660 from: 1 to: 6232 pFN21AE0965 6232 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 3575 1937 Size: 262 1376 4594 Fragments arranged by size: 4594 1376 262 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2790 2873 4295 5679 5947 1632 Size: 1158 83 1422 1384 268 1917 Fragments arranged by size: 1917 1422 1384 1158 268 83 AfeI AGC'GCT Cuts at: 3143 3143 Size: 6232 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 2103 5131 6108 1203 Size: 900 3028 977 1327 Fragments arranged by size: 3028 1327 977 900 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 3975 5547 3975 Size: 1572 4660 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 2359 2447 3541 3767 3919 6028 1493 Size: 866 88 1094 226 152 2109 1697 Fragments arranged by size: 2109 1697 1094 866 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2481 3557 1870 Size: 73 538 1076 4545 Fragments arranged by size: 4545 1076 538 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2815 2848 2815 Size: 33 6199 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3134 3553 5008 618 Size: 325 217 627 1347 419 1455 1842 Fragments arranged by size: 1842 1455 1347 627 419 325 217 BanII G_rGCy'C Cuts at: 1391 2514 3551 1391 Size: 1123 1037 4072 Fragments arranged by size: 4072 1123 1037 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2582 928 Size: 971 683 4578 Fragments arranged by size: 4578 971 683 BbvCI CC'TCA_GC Cuts at: 2774 2774 Size: 6232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2899 4124 5334 2899 Size: 1225 1210 3797 Fragments arranged by size: 3797 1225 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BfrBI ATG'CAT Cuts at: 3085 3085 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2709 6227 2709 Size: 3518 2714 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 3137 4293 5449 1790 Size: 1347 1156 1156 2573 Fragments arranged by size: 2573 1347 1156 1156 BmgBI CAC'GTC Cuts at: 1318 2197 2488 1318 Size: 879 291 5062 Fragments arranged by size: 5062 879 291 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3963 4921 442 Size: 416 53 83 399 24 2546 958 1753 Fragments arranged by size: 2546 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2456 2456 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2812 4892 1239 Size: 1573 2080 2579 Fragments arranged by size: 2579 2080 1573 Bpu10I CC'TnA_GC Cuts at: 2774 2774 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 5222 5520 4357 Size: 865 298 5069 Fragments arranged by size: 5069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 4895 882 Size: 617 627 2769 2219 Fragments arranged by size: 2769 2219 627 617 BsaAI yAC'GTr Cuts at: 493 1204 4023 493 Size: 711 2819 2702 Fragments arranged by size: 2819 2702 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2707 1186 Size: 1521 4711 BseYI C'CCAG_C Cuts at: 2319 2428 2835 5435 5931 2319 Size: 109 407 2600 496 2620 Fragments arranged by size: 2620 2600 496 407 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5659 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3098 4293 4378 5449 3098 Size: 1195 85 1071 3881 Fragments arranged by size: 3881 1195 1071 85 BsmI GAATG_Cn' Cuts at: 3085 3437 3743 3836 3085 Size: 352 306 93 5481 Fragments arranged by size: 5481 352 306 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2478 2478 Size: 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2514 3098 3137 3551 4293 4378 5449 Size: 399 724 584 39 414 742 85 1071 Cuts at: 5449 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 724 584 414 399 85 39 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 2040 2860 4121 5851 2040 Size: 820 1261 1730 2421 Fragments arranged by size: 2421 1730 1261 820 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2646 3587 844 Size: 1802 941 3489 Fragments arranged by size: 3489 1802 941 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2799 2997 3271 4727 4901 65 Size: 1183 1551 198 274 1456 174 1396 Fragments arranged by size: 1551 1456 1396 1183 274 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2567 2757 96 Size: 2471 190 3571 Fragments arranged by size: 3571 2471 190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6232 BstBI TT'CG_AA Cuts at: 2923 5036 2923 Size: 2113 4119 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstXI CCAn_nnnn'nTGG Cuts at: 3181 3256 3181 Size: 75 6157 BstYI r'GATC_y Cuts at: 1972 2709 3562 3929 4314 4331 5783 5869 5881 Size: 737 853 367 385 17 1452 86 12 Cuts at: 5881 6227 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 853 737 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2274 513 Size: 552 939 270 4471 Fragments arranged by size: 4471 939 552 270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 2165 3467 3748 4542 4562 2165 Size: 1302 281 794 20 3835 Fragments arranged by size: 3835 1302 794 281 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2509 2544 529 Size: 35 1945 35 4217 Fragments arranged by size: 4217 1945 35 35 DraI TTT'AAA Cuts at: 2452 3015 3536 2452 Size: 563 521 5148 Fragments arranged by size: 5148 563 521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2257 5239 809 Size: 507 941 2982 1802 Fragments arranged by size: 2982 1802 941 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2266 3246 3414 Size: 54 1425 50 275 18 436 980 168 Cuts at: 3414 3679 4568 6197 8 Size: 265 889 1629 43 Fragments arranged by size: 1629 1425 980 889 436 275 265 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4162 1353 Size: 2809 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3268 4819 5193 5339 387 Size: 1064 138 1679 1551 374 146 1280 Fragments arranged by size: 1679 1551 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2790 2812 2873 4295 4892 5679 5947 Size: 393 1158 22 61 1422 597 787 268 Cuts at: 5947 1239 Size: 1524 Fragments arranged by size: 1524 1422 1158 787 597 393 268 61 22 EcoO109I rG'GnC_Cy Cuts at: 2979 2979 Size: 6232 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 3145 5379 1678 Size: 1467 2234 2531 Fragments arranged by size: 2531 2234 1467 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2717 2749 2801 2833 3085 Size: 32 135 32 1145 32 52 32 252 Cuts at: 3085 3117 4873 4905 4947 4979 1373 Size: 32 1756 32 42 32 2626 Fragments arranged by size: 2626 1756 1145 252 135 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2089 3576 3822 669 Size: 988 281 151 1487 246 3079 Fragments arranged by size: 3079 1487 988 281 246 151 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2509 2616 5346 5530 1305 Size: 1204 107 2730 184 2007 Fragments arranged by size: 2730 2007 1204 184 107 MscI TGG'CCA Cuts at: 10 64 1489 1832 2268 3248 3416 10 Size: 54 1425 343 436 980 168 2826 Fragments arranged by size: 2826 1425 980 436 343 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2768 2859 3179 4190 4549 4708 Size: 648 942 660 91 320 1011 359 159 Cuts at: 4708 518 Size: 2042 Fragments arranged by size: 2042 1011 942 660 648 359 320 159 91 MspA1I CmG'CkG Cuts at: 1606 2323 2682 2839 3648 4325 5473 5718 1606 Size: 717 359 157 809 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 717 677 359 245 157 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NsiI A_TGCA'T Cuts at: 3087 3087 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 2107 3590 5135 1132 Size: 97 878 1483 1545 2229 Fragments arranged by size: 2229 1545 1483 878 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 2103 5131 2103 Size: 3028 3204 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 3384 1108 Size: 1167 1109 3956 Fragments arranged by size: 3956 1167 1109 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2634 4301 4333 5814 5846 2602 Size: 32 1667 32 1481 32 2988 Fragments arranged by size: 2988 1667 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2979 2979 Size: 6232 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3201 3802 3201 Size: 601 5631 PstI C_TGCA'G Cuts at: 830 3584 830 Size: 2754 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2323 2682 2839 2323 Size: 359 157 5716 Fragments arranged by size: 5716 359 157 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SanDI GG'GwC_CC Cuts at: 2979 2979 Size: 6232 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SfcI C'TryA_G Cuts at: 826 1045 2394 3580 4715 5396 5587 826 Size: 219 1349 1186 1135 681 191 1471 Fragments arranged by size: 1471 1349 1186 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4336 5237 5499 820 Size: 197 926 2393 901 262 1553 Fragments arranged by size: 2393 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 3028 4156 5 Size: 47 2976 1128 2081 Fragments arranged by size: 2976 2081 1128 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2004 2082 2287 2983 3671 513 Size: 552 939 78 205 696 688 3074 Fragments arranged by size: 3074 939 696 688 552 205 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2567 2757 4478 Size: 274 80 33 51 1443 590 190 1721 Cuts at: 4478 6190 96 Size: 1712 138 Fragments arranged by size: 1721 1712 1443 590 274 190 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2346 2378 2549 2581 6192 6224 2346 Size: 32 171 32 3611 32 2354 Fragments arranged by size: 3611 2354 171 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3163 3568 3163 Size: 405 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2372 3145 2372 Size: 773 5459 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 2711 4361 2711 Size: 1650 4582 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI