Restriction map for pFN21AE0965
 (Linear) (Six-base) MAPSORT of: KIEE0965.seq  Check: 5607  from: 1  to: 1527



KIEE0965.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AccI GT'mk_AC

Cuts at:      0     194    1527 
   Size:        194    1333
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785     868    1527 
   Size:        785      83     659

  Fragments arranged by size: 

                 785     659      83
 
AfeI AGC'GCT

Cuts at:      0    1138    1527 
   Size:       1138     389
 
AflIII A'CryG_T

Cuts at:      0      98    1527 
   Size:         98    1429
 
ApoI r'AATT_y

Cuts at:      0     354     442    1527 
   Size:        354      88    1085

  Fragments arranged by size: 

                1085     354      88
 
AvaI C'yCGr_G

Cuts at:      0     476    1527 
   Size:        476    1051
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     810     843    1527 
   Size:        810      33     684

  Fragments arranged by size: 

                 810     684      33
 
BanI G'GyrC_C

Cuts at:      0    1129    1527 
   Size:       1129     398
 
BanII G_rGCy'C

Cuts at:      0     509    1527 
   Size:        509    1018
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     577    1527 
   Size:        577     950
 
BbvCI CC'TCA_GC

Cuts at:      0     769    1527 
   Size:        769     758
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     894    1527 
   Size:        894     633
 
BfrBI ATG'CAT

Cuts at:      0    1080    1527 
   Size:       1080     447
 
BglII A'GATC_T

Cuts at:      0     704    1527 
   Size:        704     823
 
Bme1580I G_kGCm'C

Cuts at:      0    1132    1527 
   Size:       1132     395
 
BmgBI CAC'GTC

Cuts at:      0     192     483    1527 
   Size:        192     291    1044

  Fragments arranged by size: 

                1044     291     192
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     451    1527 
   Size:        451    1076
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     807    1527 
   Size:        807     720
 
Bpu10I CC'TnA_GC

Cuts at:      0     769    1527 
   Size:        769     758
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     121    1527 
   Size:        121    1406
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     702    1527 
   Size:        702     825
 
BseYI C'CCAG_C

Cuts at:      0     314     423     830    1527 
   Size:        314     109     407     697

  Fragments arranged by size: 

                 697     407     314     109
 
BsiHKAI G_wGCw'C

Cuts at:      0    1093    1527 
   Size:       1093     434
 
BsmI GAATG_Cn'

Cuts at:      0    1080    1432    1527 
   Size:       1080     352      95

  Fragments arranged by size: 

                1080     352      95
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     473    1527 
   Size:        473    1054
 
Bsp1286I G_dGCh'C

Cuts at:      0     509    1093    1132    1527 
   Size:        509     584      39     395

  Fragments arranged by size: 

                 584     509     395      39
 
BspHI T'CATG_A

Cuts at:      0      35     855    1527 
   Size:         35     820     672

  Fragments arranged by size: 

                 820     672      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     641    1527 
   Size:        641     886
 
BsrDI GCAATG_nn'

Cuts at:      0     794     992    1266    1527 
   Size:        794     198     274     261

  Fragments arranged by size: 

                 794     274     261     198
 
BsrGI T'GTAC_A

Cuts at:      0     562     752    1527 
   Size:        562     190     775

  Fragments arranged by size: 

                 775     562     190
 
BstBI TT'CG_AA

Cuts at:      0     918    1527 
   Size:        918     609
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1176    1251    1527 
   Size:       1176      75     276

  Fragments arranged by size: 

                1176     276      75
 
BstYI r'GATC_y

Cuts at:      0     704    1527 
   Size:        704     823
 
BtgI C'CryG_G

Cuts at:      0     269    1527 
   Size:        269    1258
 
BtsI GCAGTG_nn'

Cuts at:      0     160    1462    1527 
   Size:        160    1302      65

  Fragments arranged by size: 

                1302     160      65
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     504     539    1527 
   Size:        504      35     988

  Fragments arranged by size: 

                 988     504      35
 
DraI TTT'AAA

Cuts at:      0     447    1010    1527 
   Size:        447     563     517

  Fragments arranged by size: 

                 563     517     447
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     252    1527 
   Size:        252    1275
 
EaeI y'GGCC_r

Cuts at:      0     261    1241    1409    1527 
   Size:        261     980     168     118

  Fragments arranged by size: 

                 980     261     168     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1263    1527 
   Size:       1263     264
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785     807     868    1527 
   Size:        785      22      61     659

  Fragments arranged by size: 

                 785     659      61      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     974    1527 
   Size:        974     553
 
HaeII r_GCGC'y

Cuts at:      0    1140    1527 
   Size:       1140     387
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     712     744     796     828    1080    1112    1527 
   Size:        712      32      52      32     252      32     415

  Fragments arranged by size: 

                 712     415     252      52      32      32      32
 
HincII GTy'rAC

Cuts at:      0      84    1527 
   Size:         84    1443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     504     611    1527 
   Size:        504     107     916

  Fragments arranged by size: 

                 916     504     107
 
MscI TGG'CCA

Cuts at:      0     263    1243    1411    1527 
   Size:        263     980     168     116

  Fragments arranged by size: 

                 980     263     168     116
 
MslI CAynn'nnrTG

Cuts at:      0     103     763     854    1174    1527 
   Size:        103     660      91     320     353

  Fragments arranged by size: 

                 660     353     320     103      91
 
MspA1I CmG'CkG

Cuts at:      0     318     677     834    1527 
   Size:        318     359     157     693

  Fragments arranged by size: 

                 693     359     318     157
 
NsiI A_TGCA'T

Cuts at:      0    1082    1527 
   Size:       1082     445
 
NspI r_CATG'y

Cuts at:      0     102    1527 
   Size:        102    1425
 
PciI A'CATG_T

Cuts at:      0      98    1527 
   Size:         98    1429
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     270    1379    1527 
   Size:        270    1109     148

  Fragments arranged by size: 

                1109     270     148
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     597     629    1527 
   Size:        597      32     898

  Fragments arranged by size: 

                 898     597      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     974    1527 
   Size:        974     553
 
PsiI TTA'TAA

Cuts at:      0    1196    1527 
   Size:       1196     331
 
PvuII CAG'CTG

Cuts at:      0     318     677     834    1527 
   Size:        318     359     157     693

  Fragments arranged by size: 

                 693     359     318     157
 
SanDI GG'GwC_CC

Cuts at:      0     974    1527 
   Size:        974     553
 
SfcI C'TryA_G

Cuts at:      0     389    1527 
   Size:        389    1138
 
SspI AAT'ATT

Cuts at:      0    1023    1527 
   Size:       1023     504
 
StyI C'CwwG_G

Cuts at:      0      77     282     978    1527 
   Size:         77     205     696     549

  Fragments arranged by size: 

                 696     549     205      77
 
TatI w'GTAC_w

Cuts at:      0     562     752    1527 
   Size:        562     190     775

  Fragments arranged by size: 

                 775     562     190
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     341     373     544     576    1527 
   Size:        341      32     171      32     951

  Fragments arranged by size: 

                 951     341     171      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1158    1527 
   Size:       1158     369
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     367    1140    1527 
   Size:        367     773     387

  Fragments arranged by size: 

                 773     387     367
 
XmnI GAAnn'nnTTC

Cuts at:      0     706    1527 
   Size:        706     821
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     ApoI     AvaI     BaeI     BanI
    BanII     BbsI    BbvCI    BciVI    BfrBI    BglII Bme1580I    BmgBI
     BplI     BpmI   Bpu10I     BsaI    BseRI    BseYI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BstBI    BstXI
    BstYI     BtgI     BtsI    CspCI     DraI     DrdI     EaeI     EciI
  Eco57MI EcoO109I    HaeII    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I     NsiI     NspI     PciI    PflMI     PpiI    PpuMI     PsiI
    PvuII    SanDI     SfcI     SspI     StyI     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII    BamHI     BbeI     BcgI     BclI     BglI     BlpI     BmrI
     BmtI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     EagI     EarI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PfoI     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XhoI
     XmaI     ZraI
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