(Circular) (Six-base) MAPSORT of: pFN21AB0471.seq Check: 9832 from: 1 to: 6250 pFN21AB0471 6250 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4060 278 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2213 3593 1937 Size: 276 1380 4594 Fragments arranged by size: 4594 1380 276 Acc65I G'GTAC_C Cuts at: 3571 3571 Size: 6250 AclI AA'CG_TT Cuts at: 1563 2357 4377 4750 1563 Size: 794 2020 373 3063 Fragments arranged by size: 3063 2020 794 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2017 3444 4313 5697 5965 1632 Size: 385 1427 869 1384 268 1917 Fragments arranged by size: 1917 1427 1384 869 385 268 AflII C'TTAA_G Cuts at: 820 1017 2818 820 Size: 197 1801 4252 Fragments arranged by size: 4252 1801 197 AflIII A'CryG_T Cuts at: 1203 2397 5149 6126 1203 Size: 1194 2752 977 1327 Fragments arranged by size: 2752 1327 1194 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 3032 3091 4979 3032 Size: 59 1888 4303 Fragments arranged by size: 4303 1888 59 AleI CACnn'nnGTG Cuts at: 1166 2786 1166 Size: 1620 4630 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6250 AlwNI CAG_nnn'CTG Cuts at: 2726 3993 5565 2726 Size: 1267 1572 3411 Fragments arranged by size: 3411 1572 1267 ApaI G_GGCC'C Cuts at: 3186 3186 Size: 6250 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 2143 2462 2578 2845 3559 3785 3937 6046 Size: 650 319 116 267 714 226 152 2109 Cuts at: 6046 1493 Size: 1697 Fragments arranged by size: 2109 1697 714 650 319 267 226 152 116 AseI AT'TA_AT Cuts at: 160 1032 2448 4804 160 Size: 872 1416 2356 1606 Fragments arranged by size: 2356 1606 1416 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 3575 1870 Size: 73 1632 4545 Fragments arranged by size: 4545 1632 73 BamHI G'GATC_C Cuts at: 3385 3580 3947 3385 Size: 195 367 5688 Fragments arranged by size: 5688 367 195 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3571 5026 618 Size: 325 217 627 1784 1455 1842 Fragments arranged by size: 1842 1784 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3135 3186 3569 1391 Size: 1744 51 383 4072 Fragments arranged by size: 4072 1744 383 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5279 BbvCI CC'TCA_GC Cuts at: 3159 3159 Size: 6250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4441 4475 1903 Size: 34 50 34 2420 34 3678 Fragments arranged by size: 3678 2420 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4142 5352 4142 Size: 1210 5040 BclI T'GATC_A Cuts at: 1548 3493 1548 Size: 1945 4305 BfrBI ATG'CAT Cuts at: 3022 3022 Size: 6250 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4861 136 Size: 107 122 71 631 605 3189 1525 Fragments arranged by size: 3189 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6245 6245 Size: 6250 BlpI GC'TnA_GC Cuts at: 3667 3667 Size: 6250 Bme1580I G_kGCm'C Cuts at: 1790 3186 4311 5467 1790 Size: 1396 1125 1156 2573 Fragments arranged by size: 2573 1396 1156 1125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3070 3981 4939 Size: 416 53 83 399 24 1653 911 958 Cuts at: 4939 442 Size: 1753 Fragments arranged by size: 1753 1653 958 911 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3194 4910 1239 Size: 1955 1716 2579 Fragments arranged by size: 2579 1955 1716 Bpu10I CC'TnA_GC Cuts at: 3159 3159 Size: 6250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3196 4375 5240 5538 3196 Size: 1179 865 298 3908 Fragments arranged by size: 3908 1179 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4913 882 Size: 617 3414 2219 Fragments arranged by size: 3414 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4041 493 Size: 711 2837 2702 Fragments arranged by size: 2837 2702 711 BsaBI GATnn'nnATC Cuts at: 6244 6244 Size: 6250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4057 4439 5074 275 Size: 53 83 186 936 2524 382 635 1451 Fragments arranged by size: 2524 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2148 4683 5081 5355 5502 5913 2148 Size: 2535 398 274 147 411 2485 Fragments arranged by size: 2535 2485 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3187 3217 3224 3254 1178 Size: 30 165 30 1784 30 7 30 4174 Fragments arranged by size: 4174 1784 165 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3113 3116 3208 1186 Size: 1927 3 92 4228 Fragments arranged by size: 4228 1927 92 3 BseYI C'CCAG_C Cuts at: 3449 5453 5949 3449 Size: 2004 496 3750 Fragments arranged by size: 3750 2004 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5677 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 4461 5489 1540 Size: 275 186 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 3135 4311 4396 5467 2646 Size: 489 1176 85 1071 3429 Fragments arranged by size: 3429 1176 1071 489 85 BsmI GAATG_Cn' Cuts at: 2026 3761 3854 2026 Size: 1735 93 4422 Fragments arranged by size: 4422 1735 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3360 3360 Size: 6250 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2646 3135 3186 3569 4311 4396 5467 Size: 399 856 489 51 383 742 85 1071 Cuts at: 5467 1391 Size: 2174 Fragments arranged by size: 2174 1071 856 742 489 399 383 85 51 BspEI T'CCGG_A Cuts at: 5913 5913 Size: 6250 BspHI T'CATG_A Cuts at: 2220 4139 5869 2220 Size: 1919 1730 2601 Fragments arranged by size: 2601 1919 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2889 3120 3605 844 Size: 2045 231 485 3489 Fragments arranged by size: 3489 2045 485 231 BsrBI CCG'CTC Cuts at: 1394 1823 4137 1394 Size: 429 2314 3507 Fragments arranged by size: 3507 2314 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2135 2204 4745 4919 65 Size: 1183 887 69 2541 174 1396 Fragments arranged by size: 2541 1396 1183 887 174 69 BsrFI r'CCGG_y Cuts at: 1700 1810 4894 5081 1700 Size: 110 3084 187 2869 Fragments arranged by size: 3084 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6250 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6250 BssSI C'ACGA_G Cuts at: 2072 2312 4310 5322 2072 Size: 240 1998 1012 3000 Fragments arranged by size: 3000 1998 1012 240 BstAPI GCAn_nnn'nTGC Cuts at: 794 2726 794 Size: 1932 4318 BstBI TT'CG_AA Cuts at: 5054 5054 Size: 6250 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6250 BstXI CCAn_nnnn'nTGG Cuts at: 2905 2905 Size: 6250 BstYI r'GATC_y Cuts at: 1972 3385 3580 3947 4332 4349 5801 5887 5899 Size: 1413 195 367 385 17 1452 86 12 Cuts at: 5899 6245 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1413 385 367 346 195 86 17 12 BstZ17I GTA'TAC Cuts at: 2214 2214 Size: 6250 Bsu36I CC'TnA_GG Cuts at: 3117 3117 Size: 6250 BtgI C'CryG_G Cuts at: 513 1065 2898 3243 513 Size: 552 1833 345 3520 Fragments arranged by size: 3520 1833 552 345 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5511 Fragments arranged by size: 5511 683 56 BtsI GCAGTG_nn' Cuts at: 3766 4560 4580 3766 Size: 794 20 5436 Fragments arranged by size: 5436 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6215 DraI TTT'AAA Cuts at: 3554 3554 Size: 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5257 809 Size: 507 3941 1802 Fragments arranged by size: 3941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2388 2701 2867 Size: 54 1425 50 275 18 558 313 166 Cuts at: 2867 3417 3697 4586 6215 8 Size: 550 280 889 1629 43 Fragments arranged by size: 1629 1425 889 558 550 313 280 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3697 1537 Size: 275 1885 4090 Fragments arranged by size: 4090 1885 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3222 3360 3463 4180 1353 Size: 1869 138 103 717 3423 Fragments arranged by size: 3423 1869 717 138 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4837 5211 5357 387 Size: 1064 138 3248 374 146 1280 Fragments arranged by size: 3248 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3133 3133 Size: 6250 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 3194 3444 4313 4910 5697 5965 Size: 393 385 1177 250 869 597 787 268 Cuts at: 5965 1239 Size: 1524 Fragments arranged by size: 1524 1177 869 787 597 393 385 268 250 EcoNI CCTnn'n_nnAGG Cuts at: 3116 3190 3116 Size: 74 6176 EcoO109I rG'GnC_Cy Cuts at: 3183 3214 3466 3183 Size: 31 252 5967 Fragments arranged by size: 5967 252 31 EcoRI G'AATT_C Cuts at: 3559 3937 3559 Size: 378 5872 EcoRV GAT'ATC Cuts at: 2133 2133 Size: 6250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2692 3522 2692 Size: 830 5420 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 2993 5397 1678 Size: 1315 2404 2531 Fragments arranged by size: 2531 2404 1315 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3018 3050 3077 3109 3187 Size: 32 135 32 1446 32 27 32 78 Cuts at: 3187 3219 3224 3256 3272 3304 3473 3505 4891 Size: 32 5 32 16 32 169 32 1386 Cuts at: 4891 4923 4965 4997 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1446 1386 169 135 78 42 32 32 32 32 32 32 32 32 32 32 27 16 5 HincII GTy'rAC Cuts at: 669 1657 1938 3376 3523 3594 3840 669 Size: 988 281 1438 147 71 246 3079 Fragments arranged by size: 3079 1438 988 281 246 147 71 HindIII A'AGCT_T Cuts at: 748 748 Size: 6250 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KpnI G_GTAC'C Cuts at: 3575 3575 Size: 6250 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2871 3147 5364 5548 1305 Size: 1566 276 2217 184 2007 Fragments arranged by size: 2217 2007 1566 276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2390 2703 2869 3419 10 Size: 54 1425 343 558 313 166 550 2841 Fragments arranged by size: 2841 1425 558 550 343 313 166 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 2448 2450 2649 2786 2797 2903 Size: 648 1230 52 2 199 137 11 106 Cuts at: 2903 4208 4567 4726 518 Size: 1305 359 159 2042 Fragments arranged by size: 2042 1305 1230 648 359 199 159 137 106 52 11 2 MspA1I CmG'CkG Cuts at: 1606 2346 3423 3666 4343 5491 5736 1606 Size: 740 1077 243 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 740 677 245 243 NcoI C'CATG_G Cuts at: 513 1065 2898 513 Size: 552 1833 3865 Fragments arranged by size: 3865 1833 552 NdeI CA'TA_TG Cuts at: 387 2089 387 Size: 1702 4548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NsiI A_TGCA'T Cuts at: 3024 3024 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 2401 3608 5153 1132 Size: 97 1172 1207 1545 2229 Fragments arranged by size: 2229 1545 1207 1172 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6250 PciI A'CATG_T Cuts at: 2397 5149 2397 Size: 2752 3498 PflMI CCAn_nnn'nTGG Cuts at: 1108 3415 1108 Size: 2307 3943 PfoI T'CCnGG_A Cuts at: 3210 3210 Size: 6250 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4351 5832 5864 4319 Size: 32 1481 32 4705 Fragments arranged by size: 4705 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3214 3214 Size: 6250 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 2952 3820 2952 Size: 868 5382 PspOMI G'GGCC_C Cuts at: 3182 3182 Size: 6250 PstI C_TGCA'G Cuts at: 830 3602 830 Size: 2772 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 SacI G_AGCT'C Cuts at: 3135 3135 Size: 6250 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 4498 6210 4498 Size: 1712 4538 SfcI C'TryA_G Cuts at: 826 1045 3066 3235 3598 4733 5414 5605 826 Size: 219 2021 169 363 1135 681 191 1471 Fragments arranged by size: 2021 1471 1135 681 363 219 191 169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2818 3175 4354 5255 5517 820 Size: 197 926 875 357 1179 901 262 1553 Fragments arranged by size: 1553 1179 926 901 875 357 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6250 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 4174 5 Size: 47 4122 2081 Fragments arranged by size: 4122 2081 47 StuI AGG'CCT Cuts at: 1517 2318 3040 3053 1517 Size: 801 722 13 4714 Fragments arranged by size: 4714 801 722 13 StyI C'CwwG_G Cuts at: 513 1065 2898 3526 3689 513 Size: 552 1833 628 163 3074 Fragments arranged by size: 3074 1833 628 552 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4277 4294 4447 4632 5077 325 Size: 1255 2697 17 153 185 445 1498 Fragments arranged by size: 2697 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4496 6208 96 Size: 274 80 33 51 1443 2519 1712 138 Fragments arranged by size: 2519 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2677 2709 6210 6242 2677 Size: 32 3501 32 2685 Fragments arranged by size: 3501 2685 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3586 3586 Size: 6250 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2905 2905 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 2024 4379 2024 Size: 2355 3895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4058 276 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI