Restriction map for
pFN21AB0471
(Linear) (Six-base) MAPSORT of: KIBB0471.seq Check: 4623 from: 1 to: 1545
KIBB0471.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 0 208 1545
Size: 208 1337
AclI AA'CG_TT
Cuts at: 0 352 1545
Size: 352 1193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 1439 1545
Size: 12 1427 106
Fragments arranged by size:
1427 106 12
AflII C'TTAA_G
Cuts at: 0 813 1545
Size: 813 732
AflIII A'CryG_T
Cuts at: 0 392 1545
Size: 392 1153
AhdI GACnn_n'nnGTC
Cuts at: 0 1027 1086 1545
Size: 1027 59 459
Fragments arranged by size:
1027 459 59
AleI CACnn'nnGTG
Cuts at: 0 781 1545
Size: 781 764
AlwNI CAG_nnn'CTG
Cuts at: 0 721 1545
Size: 721 824
ApaI G_GGCC'C
Cuts at: 0 1181 1545
Size: 1181 364
ApoI r'AATT_y
Cuts at: 0 138 457 573 840 1545
Size: 138 319 116 267 705
Fragments arranged by size:
705 319 267 138 116
AseI AT'TA_AT
Cuts at: 0 443 1545
Size: 443 1102
BamHI G'GATC_C
Cuts at: 0 1380 1545
Size: 1380 165
BanII G_rGCy'C
Cuts at: 0 1130 1181 1545
Size: 1130 51 364
Fragments arranged by size:
1130 364 51
BbvCI CC'TCA_GC
Cuts at: 0 1154 1545
Size: 1154 391
BclI T'GATC_A
Cuts at: 0 1488 1545
Size: 1488 57
BfrBI ATG'CAT
Cuts at: 0 1017 1545
Size: 1017 528
Bme1580I G_kGCm'C
Cuts at: 0 1181 1545
Size: 1181 364
BmrI ACTGGGnnnn_n'
Cuts at: 0 1065 1545
Size: 1065 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1545
Size: 1189 356
Bpu10I CC'TnA_GC
Cuts at: 0 1154 1545
Size: 1154 391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1191 1545
Size: 1191 354
BsaWI w'CCGG_w
Cuts at: 0 143 1545
Size: 143 1402
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1182 1212 1219 1249 1545
Size: 1182 30 7 30 296
Fragments arranged by size:
1182 296 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1108 1111 1203 1545
Size: 1108 3 92 342
Fragments arranged by size:
1108 342 92 3
BseYI C'CCAG_C
Cuts at: 0 1444 1545
Size: 1444 101
BsiHKAI G_wGCw'C
Cuts at: 0 641 1130 1545
Size: 641 489 415
Fragments arranged by size:
641 489 415
BsmI GAATG_Cn'
Cuts at: 0 21 1545
Size: 21 1524
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1355 1545
Size: 1355 190
Bsp1286I G_dGCh'C
Cuts at: 0 641 1130 1181 1545
Size: 641 489 51 364
Fragments arranged by size:
641 489 364 51
BspHI T'CATG_A
Cuts at: 0 215 1545
Size: 215 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 884 1115 1545
Size: 884 231 430
Fragments arranged by size:
884 430 231
BsrDI GCAATG_nn'
Cuts at: 0 130 199 1545
Size: 130 69 1346
Fragments arranged by size:
1346 130 69
BssSI C'ACGA_G
Cuts at: 0 67 307 1545
Size: 67 240 1238
Fragments arranged by size:
1238 240 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 721 1545
Size: 721 824
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 1545
Size: 900 645
BstYI r'GATC_y
Cuts at: 0 1380 1545
Size: 1380 165
BstZ17I GTA'TAC
Cuts at: 0 209 1545
Size: 209 1336
Bsu36I CC'TnA_GG
Cuts at: 0 1112 1545
Size: 1112 433
BtgI C'CryG_G
Cuts at: 0 893 1238 1545
Size: 893 345 307
Fragments arranged by size:
893 345 307
EaeI y'GGCC_r
Cuts at: 0 383 696 862 1412 1545
Size: 383 313 166 550 133
Fragments arranged by size:
550 383 313 166 133
EarI CTCTTCn'nnn_
Cuts at: 0 1217 1355 1458 1545
Size: 1217 138 103 87
Fragments arranged by size:
1217 138 103 87
EcoICRI GAG'CTC
Cuts at: 0 1128 1545
Size: 1128 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 1189 1439 1545
Size: 12 1177 250 106
Fragments arranged by size:
1177 250 106 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1111 1185 1545
Size: 1111 74 360
Fragments arranged by size:
1111 360 74
EcoO109I rG'GnC_Cy
Cuts at: 0 1178 1209 1461 1545
Size: 1178 31 252 84
Fragments arranged by size:
1178 252 84 31
EcoRV GAT'ATC
Cuts at: 0 128 1545
Size: 128 1417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 687 1517 1545
Size: 687 830 28
Fragments arranged by size:
830 687 28
HaeII r_GCGC'y
Cuts at: 0 988 1545
Size: 988 557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 1072 1104 1182 1214 1219 1251
Size: 1013 32 27 32 78 32 5 32
Cuts at: 1251 1267 1299 1468 1500 1545
Size: 16 32 169 32 45
Fragments arranged by size:
1013 169 78 45 32 32 32 32
32 32 27 16 5
HincII GTy'rAC
Cuts at: 0 1371 1518 1545
Size: 1371 147 27
Fragments arranged by size:
1371 147 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1142 1545
Size: 866 276 403
Fragments arranged by size:
866 403 276
MscI TGG'CCA
Cuts at: 0 385 698 864 1414 1545
Size: 385 313 166 550 131
Fragments arranged by size:
550 385 313 166 131
MslI CAynn'nnrTG
Cuts at: 0 391 443 445 644 781 792 898 1545
Size: 391 52 2 199 137 11 106 647
Fragments arranged by size:
647 391 199 137 106 52 11 2
MspA1I CmG'CkG
Cuts at: 0 341 1418 1545
Size: 341 1077 127
Fragments arranged by size:
1077 341 127
NcoI C'CATG_G
Cuts at: 0 893 1545
Size: 893 652
NdeI CA'TA_TG
Cuts at: 0 84 1545
Size: 84 1461
NsiI A_TGCA'T
Cuts at: 0 1019 1545
Size: 1019 526
NspI r_CATG'y
Cuts at: 0 396 1545
Size: 396 1149
PciI A'CATG_T
Cuts at: 0 392 1545
Size: 392 1153
PflMI CCAn_nnn'nTGG
Cuts at: 0 1410 1545
Size: 1410 135
PfoI T'CCnGG_A
Cuts at: 0 1205 1545
Size: 1205 340
PpuMI rG'GwC_Cy
Cuts at: 0 1209 1545
Size: 1209 336
PsiI TTA'TAA
Cuts at: 0 947 1545
Size: 947 598
PspOMI G'GGCC_C
Cuts at: 0 1177 1545
Size: 1177 368
SacI G_AGCT'C
Cuts at: 0 1130 1545
Size: 1130 415
SfcI C'TryA_G
Cuts at: 0 1061 1230 1545
Size: 1061 169 315
Fragments arranged by size:
1061 315 169
SmlI C'TyrA_G
Cuts at: 0 813 1170 1545
Size: 813 357 375
Fragments arranged by size:
813 375 357
StuI AGG'CCT
Cuts at: 0 313 1035 1048 1545
Size: 313 722 13 497
Fragments arranged by size:
722 497 313 13
StyI C'CwwG_G
Cuts at: 0 893 1521 1545
Size: 893 628 24
Fragments arranged by size:
893 628 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 672 704 1545
Size: 672 32 841
Fragments arranged by size:
841 672 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 1545
Size: 900 645
XmnI GAAnn'nnTTC
Cuts at: 0 19 1545
Size: 19 1526
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AleI AlwNI
ApaI ApoI AseI BamHI BanII BbvCI BclI BfrBI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PciI PflMI PfoI PpuMI PsiI PspOMI
SacI SfcI SmlI StuI StyI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AlfI AloI ApaLI
AscI AsiSI AvaI AvrII BaeI BanI BbeI BbsI
BcgI BciVI BglI BglII BlpI BmgBI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BspEI
BsrBI BsrFI BsrGI BssHII BstBI BstEII BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PshAI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XhoI XmaI ZraI