(Circular) (Six-base) MAPSORT of: pFN21AB9989.seq Check: 1946 from: 1 to: 6442 pFN21AB9989 6442 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 1937 Size: 1848 4594 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2613 4505 5889 6157 1632 Size: 981 1892 1384 268 1917 Fragments arranged by size: 1917 1892 1384 981 268 AflII C'TTAA_G Cuts at: 820 1017 3071 820 Size: 197 2054 4191 Fragments arranged by size: 4191 2054 197 AflIII A'CryG_T Cuts at: 1203 2728 3707 5341 6318 1203 Size: 1525 979 1634 977 1327 Fragments arranged by size: 1634 1525 1327 979 977 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2334 1184 Size: 1150 5292 AlwNI CAG_nnn'CTG Cuts at: 4185 5757 4185 Size: 1572 4870 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 2844 3143 3504 3751 3977 4129 6238 1493 Size: 1351 299 361 247 226 152 2109 1697 Fragments arranged by size: 2109 1697 1351 361 299 247 226 152 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 3767 1870 Size: 73 1824 4545 Fragments arranged by size: 4545 1824 73 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3763 5218 618 Size: 325 217 627 1976 1455 1842 Fragments arranged by size: 1976 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3761 1391 Size: 2370 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2723 3623 928 Size: 971 824 900 3747 Fragments arranged by size: 3747 971 900 824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2151 2185 4633 4667 1903 Size: 34 214 34 2448 34 3678 Fragments arranged by size: 3678 2448 214 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4334 5544 4334 Size: 1210 5232 BclI T'GATC_A Cuts at: 1548 1548 Size: 6442 BfrBI ATG'CAT Cuts at: 2417 2561 2417 Size: 144 6298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5053 136 Size: 107 122 71 631 605 337 3044 1525 Fragments arranged by size: 3044 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6437 6437 Size: 6442 BlpI GC'TnA_GC Cuts at: 3859 3859 Size: 6442 Bme1580I G_kGCm'C Cuts at: 1790 4503 5659 1790 Size: 2713 1156 2573 Fragments arranged by size: 2713 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4173 5131 Size: 416 53 83 399 24 1958 798 958 Cuts at: 5131 442 Size: 1753 Fragments arranged by size: 1958 1753 958 798 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3434 1056 Size: 2378 4064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 5102 1239 Size: 990 2873 2579 Fragments arranged by size: 2873 2579 990 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4567 5432 5730 4567 Size: 865 298 5279 Fragments arranged by size: 5279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2255 3728 5105 882 Size: 617 756 1473 1377 2219 Fragments arranged by size: 2219 1473 1377 756 617 BsaAI yAC'GTr Cuts at: 493 1204 4233 493 Size: 711 3029 2702 Fragments arranged by size: 3029 2702 711 BsaBI GATnn'nnATC Cuts at: 2439 6436 2439 Size: 3997 2445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2874 4249 4631 5266 Size: 53 83 186 936 1341 1375 382 635 Cuts at: 5266 275 Size: 1451 Fragments arranged by size: 1451 1375 1341 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2176 4875 5273 5547 5694 6105 2176 Size: 2699 398 274 147 411 2513 Fragments arranged by size: 2699 2513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3219 3249 3397 3427 1178 Size: 30 165 30 1816 30 148 30 4193 Fragments arranged by size: 4193 1816 165 148 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2726 1186 Size: 1540 4902 BseYI C'CCAG_C Cuts at: 3182 5645 6141 3182 Size: 2463 496 3483 Fragments arranged by size: 3483 2463 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2901 3072 3257 3690 1163 Size: 573 1165 171 185 433 3915 Fragments arranged by size: 3915 1165 573 433 185 171 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3892 4653 5681 1540 Size: 275 186 1891 761 1028 2301 Fragments arranged by size: 2301 1891 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3311 3475 4503 4588 5659 3311 Size: 164 1028 85 1071 4094 Fragments arranged by size: 4094 1071 1028 164 85 BsmI GAATG_Cn' Cuts at: 3953 4046 3953 Size: 93 6349 BsmBI CGTCTCn'nnnn_ Cuts at: 2154 2154 Size: 6442 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3311 3475 3761 4503 4588 5659 1391 Size: 399 1521 164 286 742 85 1071 2174 Fragments arranged by size: 2174 1521 1071 742 399 286 164 85 BspEI T'CCGG_A Cuts at: 2176 6105 2176 Size: 3929 2513 BspHI T'CATG_A Cuts at: 3649 3685 4331 6061 3649 Size: 36 646 1730 4030 Fragments arranged by size: 4030 1730 646 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2748 3797 844 Size: 1904 1049 3489 Fragments arranged by size: 3489 1904 1049 BsrBI CCG'CTC Cuts at: 1394 1823 4329 1394 Size: 429 2506 3507 Fragments arranged by size: 3507 2506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3215 4937 5111 65 Size: 1183 1967 1722 174 1396 Fragments arranged by size: 1967 1722 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5086 5273 1700 Size: 110 3276 187 2869 Fragments arranged by size: 3276 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 4502 5514 4502 Size: 1012 5430 BstAPI GCAn_nnn'nTGC Cuts at: 794 3394 3533 794 Size: 2600 139 3703 Fragments arranged by size: 3703 2600 139 BstBI TT'CG_AA Cuts at: 3220 5246 3220 Size: 2026 4416 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6442 BstYI r'GATC_y Cuts at: 1972 3772 4139 4524 4541 5993 6079 6091 6437 Size: 1800 367 385 17 1452 86 12 346 Cuts at: 6437 1972 Size: 1977 Fragments arranged by size: 1977 1800 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2778 2778 Size: 6442 BtgI C'CryG_G Cuts at: 513 1065 2588 2928 513 Size: 552 1523 340 4027 Fragments arranged by size: 4027 1523 552 340 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5703 Fragments arranged by size: 5703 683 56 BtsI GCAGTG_nn' Cuts at: 2163 2169 3408 3678 3958 4752 4772 2163 Size: 6 1239 270 280 794 20 3833 Fragments arranged by size: 3833 1239 794 280 270 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2583 2618 529 Size: 35 2019 35 4353 Fragments arranged by size: 4353 2019 35 35 DraI TTT'AAA Cuts at: 2548 3643 3746 2548 Size: 1095 103 5244 Fragments arranged by size: 5244 1095 103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2228 5449 809 Size: 507 912 3221 1802 Fragments arranged by size: 3221 1802 912 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2905 3889 Size: 54 1425 50 275 18 198 877 984 Cuts at: 3889 4778 6407 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 984 889 877 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3889 1537 Size: 275 2077 4090 Fragments arranged by size: 4090 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 2691 4372 1353 Size: 1278 60 1681 3423 Fragments arranged by size: 3423 1681 1278 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5029 5403 5549 387 Size: 1064 138 3440 374 146 1280 Fragments arranged by size: 3440 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2613 4505 5102 5889 6157 1239 Size: 393 597 384 1892 597 787 268 1524 Fragments arranged by size: 1892 1524 787 597 597 393 384 268 EcoNI CCTnn'n_nnAGG Cuts at: 3061 3061 Size: 6442 EcoO109I rG'GnC_Cy Cuts at: 2760 2760 Size: 6442 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 EcoRV GAT'ATC Cuts at: 3016 3349 3016 Size: 333 6109 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2412 2740 2412 Size: 328 6114 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2974 5589 1678 Size: 1296 2615 2531 Fragments arranged by size: 2615 2531 1296 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 3397 3429 5083 Size: 32 135 32 436 32 1357 32 1654 Cuts at: 5083 5115 5157 5189 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1654 1357 436 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3268 3786 4032 669 Size: 988 281 867 463 518 246 3079 Fragments arranged by size: 3079 988 867 518 463 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5556 5740 1305 Size: 4251 184 2007 Fragments arranged by size: 4251 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2907 10 Size: 54 1425 343 1075 3545 Fragments arranged by size: 3545 1425 1075 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2364 3684 4400 4759 4918 518 Size: 648 1198 1320 716 359 159 2042 Fragments arranged by size: 2042 1320 1198 716 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 3858 4535 5683 5928 1606 Size: 489 1763 677 1148 245 2120 Fragments arranged by size: 2120 1763 1148 677 489 245 NcoI C'CATG_G Cuts at: 513 1065 2588 2928 513 Size: 552 1523 340 4027 Fragments arranged by size: 4027 1523 552 340 NdeI CA'TA_TG Cuts at: 387 2407 3519 387 Size: 2020 1112 3310 Fragments arranged by size: 3310 2020 1112 NheI G'CTAG_C Cuts at: 1052 3430 1052 Size: 2378 4064 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NsiI A_TGCA'T Cuts at: 2419 2563 2419 Size: 144 6298 NspI r_CATG'y Cuts at: 1132 1229 2732 3438 3711 3800 5345 1132 Size: 97 1503 706 273 89 1545 2229 Fragments arranged by size: 2229 1545 1503 706 273 97 89 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 2728 3707 5341 2728 Size: 979 1634 3829 Fragments arranged by size: 3829 1634 979 PflMI CCAn_nnn'nTGG Cuts at: 1108 3445 1108 Size: 2337 4105 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2760 2760 Size: 6442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PstI C_TGCA'G Cuts at: 830 3034 3403 3794 830 Size: 2204 369 391 3478 Fragments arranged by size: 3478 2204 391 369 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SapI GCTCTTCn'nnn_ Cuts at: 2631 2631 Size: 6442 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SfcI C'TryA_G Cuts at: 826 1045 2469 3030 3399 3631 3790 4925 5606 Size: 219 1424 561 369 232 159 1135 681 Cuts at: 5606 5797 826 Size: 191 1471 Fragments arranged by size: 1471 1424 1135 681 561 369 232 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3071 4546 5447 5709 820 Size: 197 926 1128 1475 901 262 1553 Fragments arranged by size: 1553 1475 1128 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 3438 3800 1132 Size: 2306 362 3774 Fragments arranged by size: 3774 2306 362 SspI AAT'ATT Cuts at: 5 52 2569 4366 5 Size: 47 2517 1797 2081 Fragments arranged by size: 2517 2081 1797 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6442 StyI C'CwwG_G Cuts at: 513 1065 2588 2928 3881 513 Size: 552 1523 340 953 3074 Fragments arranged by size: 3074 1523 953 552 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4469 4486 4639 4824 5269 325 Size: 1255 2889 17 153 185 445 1498 Fragments arranged by size: 2889 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3366 4688 6400 Size: 274 80 33 51 1443 1389 1322 1712 Cuts at: 6400 96 Size: 138 Fragments arranged by size: 1712 1443 1389 1322 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3219 3251 6402 6434 3219 Size: 32 3151 32 3227 Fragments arranged by size: 3227 3151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3409 1316 Size: 2093 4349 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3280 3423 3280 Size: 143 6299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 4571 4571 Size: 6442 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI