Restriction map for pFN21AB9989
 (Linear) (Six-base) MAPSORT of: KIBB9989.seq  Check: 2564  from: 1  to: 1737



KIBB9989.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     608    1737 
   Size:        608    1129
 
AflII C'TTAA_G

Cuts at:      0    1066    1737 
   Size:       1066     671
 
AflIII A'CryG_T

Cuts at:      0     723    1702    1737 
   Size:        723     979      35

  Fragments arranged by size: 

                 979     723      35
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     329    1737 
   Size:        329    1408
 
ApoI r'AATT_y

Cuts at:      0     839    1138    1499    1737 
   Size:        839     299     361     238

  Fragments arranged by size: 

                 839     361     299     238
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     718    1618    1737 
   Size:        718     900     119

  Fragments arranged by size: 

                 900     718     119
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     146     180    1737 
   Size:        146      34    1557

  Fragments arranged by size: 

                1557     146      34
 
BfrBI ATG'CAT

Cuts at:      0     412     556    1737 
   Size:        412     144    1181

  Fragments arranged by size: 

                1181     412     144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1370    1737 
   Size:       1370     367
 
BmtI G_CTAG'C

Cuts at:      0    1429    1737 
   Size:       1429     308
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224    1737 
   Size:        224    1513
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     250    1723    1737 
   Size:        250    1473      14

  Fragments arranged by size: 

                1473     250      14
 
BsaBI GATnn'nnATC

Cuts at:      0     434    1737 
   Size:        434    1303
 
BsaHI Gr'CG_yC

Cuts at:      0     869    1737 
   Size:        869     868
 
BsaWI w'CCGG_w

Cuts at:      0     171    1737 
   Size:        171    1566
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1214    1244    1392    1422    1737 
   Size:       1214      30     148      30     315

  Fragments arranged by size: 

                1214     315     148      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     721    1737 
   Size:        721    1016
 
BseYI C'CCAG_C

Cuts at:      0    1177    1737 
   Size:       1177     560
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    1067    1252    1685    1737 
   Size:        896     171     185     433      52

  Fragments arranged by size: 

                 896     433     185     171      52
 
BsiHKAI G_wGCw'C

Cuts at:      0    1306    1470    1737 
   Size:       1306     164     267

  Fragments arranged by size: 

                1306     267     164
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     149    1737 
   Size:        149    1588
 
Bsp1286I G_dGCh'C

Cuts at:      0    1306    1470    1737 
   Size:       1306     164     267

  Fragments arranged by size: 

                1306     267     164
 
BspEI T'CCGG_A

Cuts at:      0     171    1737 
   Size:        171    1566
 
BspHI T'CATG_A

Cuts at:      0    1644    1680    1737 
   Size:       1644      36      57

  Fragments arranged by size: 

                1644      57      36
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     743    1737 
   Size:        743     994
 
BsrDI GCAATG_nn'

Cuts at:      0    1210    1737 
   Size:       1210     527
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1389    1528    1737 
   Size:       1389     139     209

  Fragments arranged by size: 

                1389     209     139
 
BstBI TT'CG_AA

Cuts at:      0    1215    1737 
   Size:       1215     522
 
Bsu36I CC'TnA_GG

Cuts at:      0     773    1737 
   Size:        773     964
 
BtgI C'CryG_G

Cuts at:      0     583     923    1737 
   Size:        583     340     814

  Fragments arranged by size: 

                 814     583     340
 
BtsI GCAGTG_nn'

Cuts at:      0     158     164    1403    1673    1737 
   Size:        158       6    1239     270      64

  Fragments arranged by size: 

                1239     270     158      64       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     578     613    1737 
   Size:        578      35    1124

  Fragments arranged by size: 

                1124     578      35
 
DraI TTT'AAA

Cuts at:      0     543    1638    1737 
   Size:        543    1095      99

  Fragments arranged by size: 

                1095     543      99
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     223    1737 
   Size:        223    1514
 
EaeI y'GGCC_r

Cuts at:      0      23     900    1737 
   Size:         23     877     837

  Fragments arranged by size: 

                 877     837      23
 
EarI CTCTTCn'nnn_

Cuts at:      0     626     686    1737 
   Size:        626      60    1051

  Fragments arranged by size: 

                1051     626      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     608    1737 
   Size:        224     384    1129

  Fragments arranged by size: 

                1129     384     224
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1056    1737 
   Size:       1056     681
 
EcoO109I rG'GnC_Cy

Cuts at:      0     755    1737 
   Size:        755     982
 
EcoRV GAT'ATC

Cuts at:      0    1011    1344    1737 
   Size:       1011     333     393

  Fragments arranged by size: 

                1011     393     333
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     407     735    1737 
   Size:        407     328    1002

  Fragments arranged by size: 

                1002     407     328
 
HaeII r_GCGC'y

Cuts at:      0     969    1737 
   Size:        969     768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       3      35    1392    1424    1737 
   Size:          3      32    1357      32     313

  Fragments arranged by size: 

                1357     313      32      32       3
 
HincII GTy'rAC

Cuts at:      0     800    1263    1737 
   Size:        800     463     474

  Fragments arranged by size: 

                 800     474     463
 
MscI TGG'CCA

Cuts at:      0     902    1737 
   Size:        902     835
 
MslI CAynn'nnrTG

Cuts at:      0     359    1679    1737 
   Size:        359    1320      58

  Fragments arranged by size: 

                1320     359      58
 
MspA1I CmG'CkG

Cuts at:      0      90    1737 
   Size:         90    1647
 
NcoI C'CATG_G

Cuts at:      0     583     923    1737 
   Size:        583     340     814

  Fragments arranged by size: 

                 814     583     340
 
NdeI CA'TA_TG

Cuts at:      0     402    1514    1737 
   Size:        402    1112     223

  Fragments arranged by size: 

                1112     402     223
 
NheI G'CTAG_C

Cuts at:      0    1425    1737 
   Size:       1425     312
 
NsiI A_TGCA'T

Cuts at:      0     414     558    1737 
   Size:        414     144    1179

  Fragments arranged by size: 

                1179     414     144
 
NspI r_CATG'y

Cuts at:      0     727    1433    1706    1737 
   Size:        727     706     273      31

  Fragments arranged by size: 

                 727     706     273      31
 
PciI A'CATG_T

Cuts at:      0     723    1702    1737 
   Size:        723     979      35

  Fragments arranged by size: 

                 979     723      35
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1440    1737 
   Size:       1440     297
 
PpuMI rG'GwC_Cy

Cuts at:      0     755    1737 
   Size:        755     982
 
PstI C_TGCA'G

Cuts at:      0    1029    1398    1737 
   Size:       1029     369     339

  Fragments arranged by size: 

                1029     369     339
 
SapI GCTCTTCn'nnn_

Cuts at:      0     626    1737 
   Size:        626    1111
 
SfcI C'TryA_G

Cuts at:      0     464    1025    1394    1626    1737 
   Size:        464     561     369     232     111

  Fragments arranged by size: 

                 561     464     369     232     111
 
SmlI C'TyrA_G

Cuts at:      0    1066    1737 
   Size:       1066     671
 
SphI G_CATG'C

Cuts at:      0    1433    1737 
   Size:       1433     304
 
SspI AAT'ATT

Cuts at:      0     564    1737 
   Size:        564    1173
 
StyI C'CwwG_G

Cuts at:      0     583     923    1737 
   Size:        583     340     814

  Fragments arranged by size: 

                 814     583     340
 
TatI w'GTAC_w

Cuts at:      0    1361    1737 
   Size:       1361     376
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1214    1246    1737 
   Size:       1214      32     491

  Fragments arranged by size: 

                1214     491      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1404    1737 
   Size:       1404     333
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1275    1418    1737 
   Size:       1275     143     319

  Fragments arranged by size: 

                1275     319     143
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI     ApoI     BbsI     BcgI    BfrBI
     BmrI     BmtI     BpmI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI   BstAPI    BstBI   Bsu36I     BtgI     BtsI    CspCI
     DraI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI    HaeII    Hin4I   HincII     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NsiI     NspI     PciI    PflMI    PpuMI     PstI
     SapI     SfcI     SmlI     SphI     SspI     StyI     TatI     TstI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BplI
   Bpu10I    BpuEI    BsaAI    BsiEI    BsiWI     BsmI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     ClaI   DraIII     EagI     EciI  EcoICRI    EcoRI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI   NgoMIV     NotI     NruI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII     XbaI     XhoI     XmaI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com