Restriction map for
pFN21AB6242
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII AccII AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
CelII CfoI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV EcoT22I FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1754 GGTACC G^GTACC
AcyI 1711 GACGCC G[GA]^CG[CT]C
AhdI 1948 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1480 GTGCAC G^TGCAC
AlwNI 454 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1480 GTGCAC G^TGCAC
Asp718I 1754 GGTACC G^GTACC
AspEI 1948 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 773 GTATCC GTATCC(6/5)
BclI 1175 TGATCA T^GATCA
BglII 1491 AGATCT A^GATCT
BsaBI 1141 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1711 GACGCC G[GA]^CG[CT]C
BsaWI 1541 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1205 TGTACA T^GTACA
BspDI 212 ATCGAT AT^CGAT
BspMI 1074 ACCTGC ACCTGC(4/8)
BsrDI 267 CATTGC GCAATG(2/0)
BsrGI 1205 TGTACA T^GTACA
BstAPI 1117 GCAATTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 880 GGTAACC G^GT[ACGT]ACC
BstPI 880 GGTAACC G^GT[ACGT]ACC
ClaI 212 ATCGAT AT^CGAT
CpoI 1538 CGGACCG CG^G[AT]CCG
Eam1105I 1948 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 813 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 880 GGTAACC G^GT[ACGT]ACC
FauI 115 GCGGG CCCGC(4/6)
FbaI 1175 TGATCA T^GATCA
HgaI 1711 GACGC GACGC(5/10)
Hin1I 1711 GACGCC G[GA]^CG[CT]C
HincII 188 GTCAAC GT[CT]^[GA]AC
HindII 188 GTCAAC GT[CT]^[GA]AC
KpnI 1754 GGTACC GGTAC^C
MaeII 1587 ACGT A^CGT
MamI 1141 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1519 CAGCTG C[AC]G^C[GT]G
NaeI 1811 GCCGGC GCC^GGC
NgoMIV 1811 GCCGGC G^CCGGC
PvuII 1519 CAGCTG CAG^CTG
RsrII 1538 CGGACCG CG^G[AT]CCG
SacI 577 GAGCTC GAGCT^C
SmlI 1943 CTCAAG C^T[CT][GA]AG
SspBI 1205 TGTACA T^GTACA
StuI 446 AGGCCT AGG^CCT
XcmI 464 CCAGCGAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 1050 GTCTAC GT^[AC][GT]AC
1956 GTCTAC
AspI 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1696 GACCTCGTC
BalI 323 TGGCCA TGG^CCA
461 TGGCCA
BanI 33 GGTGCC G^G[CT][GA]CC
1754 GGTACC
BanII 577 GAGCTC G[GA]GC[CT]^C
1776 GAGCCC
BcnI 155 CCGGG CC^[GC]GG
254 CCCGG
BsgI 1787 CTGCAC GTGCAG(16/14)
1487 GTGCAG
BsrFI 884 ACCGGC [GA]^CCGG[CT]
1811 GCCGGC
Cfr10I 884 ACCGGC [GA]^CCGG[CT]
1811 GCCGGC
DsaI 29 CCACGG C^C[GA][CT]GG
1631 CCGTGG
EaeI 323 TGGCCA [CT]^GGCC[GA]
461 TGGCCA
EarI 1987 GAAGAG CTCTTC(1/4)
2008 GAAGAG
Ksp632I 1987 GAAGAG CTCTTC(1/4)
2008 GAAGAG
MluNI 323 TGGCCA TGG^CCA
461 TGGCCA
MscI 323 TGGCCA TGG^CCA
461 TGGCCA
NciI 155 CCGGG CC^[GC]GG
254 CCCGG
PflFI 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1696 GACCTCGTC
PstI 1034 CTGCAG CTGCA^G
1917 CTGCAG
SfaNI 564 GATGC GCATC(5/9)
744 GATGC
Tth111I 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1696 GACCTCGTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BamHI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PshAI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII