(Circular) (Six-base) MAPSORT of: pFN21AE0773.seq Check: 4802 from: 1 to: 5737 pFN21AE0773 5737 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2406 2406 Size: 5737 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3080 1937 Size: 1143 4594 Acc65I G'GTAC_C Cuts at: 3058 3058 Size: 5737 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2666 3800 5184 5452 1632 Size: 1034 1134 1384 268 1917 Fragments arranged by size: 1917 1384 1134 1034 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5540 AflIII A'CryG_T Cuts at: 1203 2274 4636 5613 1203 Size: 1071 2362 977 1327 Fragments arranged by size: 2362 1327 1071 977 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 4466 4466 Size: 5737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2801 1184 Size: 1617 4120 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2861 2893 2861 Size: 32 5705 AlwNI CAG_nnn'CTG Cuts at: 3480 5052 3480 Size: 1572 4165 ApaLI G'TGCA_C Cuts at: 3794 4950 3794 Size: 1156 4581 ApoI r'AATT_y Cuts at: 1493 3046 3272 3424 5533 1493 Size: 1553 226 152 2109 1697 Fragments arranged by size: 2109 1697 1553 226 152 AseI AT'TA_AT Cuts at: 160 1032 4291 160 Size: 872 3259 1606 Fragments arranged by size: 3259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 2286 3062 1870 Size: 73 343 776 4545 Fragments arranged by size: 4545 776 343 73 BamHI G'GATC_C Cuts at: 3067 3434 3067 Size: 367 5370 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2708 3058 4513 618 Size: 325 217 627 921 350 1455 1842 Fragments arranged by size: 1842 1455 921 627 350 325 217 BanII G_rGCy'C Cuts at: 1391 2490 2634 2905 3056 1391 Size: 1099 144 271 151 4072 Fragments arranged by size: 4072 1099 271 151 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2702 928 Size: 971 803 3963 Fragments arranged by size: 3963 971 803 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2362 2396 3928 3962 1903 Size: 34 425 34 1532 34 3678 Fragments arranged by size: 3678 1532 425 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3629 4839 3629 Size: 1210 4527 BclI T'GATC_A Cuts at: 1548 1548 Size: 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4348 136 Size: 107 122 71 631 605 2676 1525 Fragments arranged by size: 2676 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5732 5732 Size: 5737 BlpI GC'TnA_GC Cuts at: 3154 3154 Size: 5737 Bme1580I G_kGCm'C Cuts at: 1790 2895 3798 4954 1790 Size: 1105 903 1156 2573 Fragments arranged by size: 2573 1156 1105 903 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5737 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3468 4426 442 Size: 416 53 83 399 24 2051 958 1753 Fragments arranged by size: 2051 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4397 1239 Size: 3158 2579 Bpu10I CC'TnA_GC Cuts at: 2066 2927 2066 Size: 861 4876 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2303 2618 3862 4727 5025 2303 Size: 315 1244 865 298 3015 Fragments arranged by size: 3015 1244 865 315 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2837 2971 4400 882 Size: 617 1338 134 1429 2219 Fragments arranged by size: 2219 1429 1338 617 134 BsaAI yAC'GTr Cuts at: 493 1204 3528 493 Size: 711 2324 2702 Fragments arranged by size: 2702 2324 711 BsaBI GATnn'nnATC Cuts at: 2384 5731 2384 Size: 3347 2390 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2245 3544 3926 4561 Size: 53 83 186 936 712 1299 382 635 Cuts at: 4561 275 Size: 1451 Fragments arranged by size: 1451 1299 936 712 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2091 2442 4170 4568 4842 4989 5400 2091 Size: 351 1728 398 274 147 411 2428 Fragments arranged by size: 2428 1728 411 398 351 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 1178 Size: 30 165 30 1497 30 3985 Fragments arranged by size: 3985 1497 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2461 2564 2704 1186 Size: 1275 103 140 4219 Fragments arranged by size: 4219 1275 140 103 BseYI C'CCAG_C Cuts at: 2079 4940 5436 2079 Size: 2861 496 2380 Fragments arranged by size: 2861 2380 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2100 2384 2497 1163 Size: 573 364 284 113 4403 Fragments arranged by size: 4403 573 364 284 113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2044 3187 3948 4976 1540 Size: 275 186 43 1143 761 1028 2301 Fragments arranged by size: 2301 1143 1028 761 275 186 43 BsiHKAI G_wGCw'C Cuts at: 2072 2490 3798 3883 4954 2072 Size: 418 1308 85 1071 2855 Fragments arranged by size: 2855 1308 1071 418 85 BsmI GAATG_Cn' Cuts at: 3248 3341 3248 Size: 93 5644 BsmBI CGTCTCn'nnnn_ Cuts at: 2426 2426 Size: 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2490 2634 2895 2905 3056 3798 Size: 399 282 418 144 261 10 151 742 Cuts at: 3798 3883 4954 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 418 399 282 261 151 144 85 10 BspEI T'CCGG_A Cuts at: 2091 5400 2091 Size: 3309 2428 BspHI T'CATG_A Cuts at: 2127 3626 5356 2127 Size: 1499 1730 2508 Fragments arranged by size: 2508 1730 1499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 2730 3092 844 Size: 1562 324 362 3489 Fragments arranged by size: 3489 1562 362 324 BsrBI CCG'CTC Cuts at: 1394 1823 2136 3624 1394 Size: 429 313 1488 3507 Fragments arranged by size: 3507 1488 429 313 BsrDI GCAATG_nn' Cuts at: 65 1248 4232 4406 65 Size: 1183 2984 174 1396 Fragments arranged by size: 2984 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4381 4568 1700 Size: 110 2571 187 2869 Fragments arranged by size: 2869 2571 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 3797 4809 3797 Size: 1012 4725 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 4163 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5737 BstXI CCAn_nnnn'nTGG Cuts at: 2545 2545 Size: 5737 BstYI r'GATC_y Cuts at: 1972 2451 2767 3067 3434 3819 3836 5288 5374 Size: 479 316 300 367 385 17 1452 86 Cuts at: 5374 5386 5732 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 479 385 367 346 316 300 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2687 2687 Size: 5737 BtgI C'CryG_G Cuts at: 513 1065 2004 2479 2578 2895 513 Size: 552 939 475 99 317 3355 Fragments arranged by size: 3355 939 552 475 317 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4998 Fragments arranged by size: 4998 683 56 BtsI GCAGTG_nn' Cuts at: 2194 3253 4047 4067 2194 Size: 1059 794 20 3864 Fragments arranged by size: 3864 1059 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 3041 3041 Size: 5737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4744 809 Size: 507 3428 1802 Fragments arranged by size: 3428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2164 3184 Size: 54 1425 50 275 18 211 123 1020 Cuts at: 3184 4073 5702 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1020 889 275 211 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2041 3184 1537 Size: 275 229 1143 4090 Fragments arranged by size: 4090 1143 275 229 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 2501 3667 1353 Size: 980 168 1166 3423 Fragments arranged by size: 3423 1166 980 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4324 4698 4844 387 Size: 1064 138 2735 374 146 1280 Fragments arranged by size: 2735 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2488 2488 Size: 5737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2666 3800 4397 5184 5452 1239 Size: 393 1034 1134 597 787 268 1524 Fragments arranged by size: 1524 1134 1034 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2686 2960 2686 Size: 274 5463 EcoO109I rG'GnC_Cy Cuts at: 2323 2908 2323 Size: 585 5152 EcoRI G'AATT_C Cuts at: 3046 3424 3046 Size: 378 5359 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2344 2344 Size: 5737 FspI TGC'GCA Cuts at: 2372 4243 2372 Size: 1871 3866 HaeII r_GCGC'y Cuts at: 1678 2318 4884 1678 Size: 640 2566 2531 Fragments arranged by size: 2566 2531 640 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2230 2262 2816 2848 2900 Size: 32 135 32 658 32 554 32 52 Cuts at: 2900 2932 4378 4410 4452 4484 1373 Size: 32 1446 32 42 32 2626 Fragments arranged by size: 2626 1446 658 554 135 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5737 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KpnI G_GTAC'C Cuts at: 3062 3062 Size: 5737 MfeI C'AATT_G Cuts at: 2223 3336 2223 Size: 1113 4624 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4851 5035 1305 Size: 3546 184 2007 Fragments arranged by size: 3546 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2166 10 Size: 54 1425 343 334 3581 Fragments arranged by size: 3581 1425 343 334 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 2783 3695 4054 4213 518 Size: 648 1377 240 912 359 159 2042 Fragments arranged by size: 2042 1377 912 648 359 240 159 MspA1I CmG'CkG Cuts at: 1606 2156 2368 2481 3153 3830 4978 5223 1606 Size: 550 212 113 672 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 550 245 212 113 NcoI C'CATG_G Cuts at: 513 1065 2004 2578 513 Size: 552 939 574 3672 Fragments arranged by size: 3672 939 574 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 2678 2752 3095 4640 1132 Size: 97 1449 74 343 1545 2229 Fragments arranged by size: 2229 1545 1449 343 97 74 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5737 PciI A'CATG_T Cuts at: 4636 4636 Size: 5737 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5737 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 5319 5351 3806 Size: 32 1481 32 4192 Fragments arranged by size: 4192 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 2908 2323 Size: 585 5152 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 3307 3307 Size: 5737 PstI C_TGCA'G Cuts at: 830 2505 3089 830 Size: 1675 584 3478 Fragments arranged by size: 3478 1675 584 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 PvuII CAG'CTG Cuts at: 2156 2368 2156 Size: 212 5525 SacI G_AGCT'C Cuts at: 2490 2490 Size: 5737 SacII CC_GC'GG Cuts at: 2482 2482 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SapI GCTCTTCn'nnn_ Cuts at: 2501 2501 Size: 5737 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SfcI C'TryA_G Cuts at: 826 1045 2501 3085 4220 4901 5092 826 Size: 219 1456 584 1135 681 191 1471 Fragments arranged by size: 1471 1456 1135 681 584 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 3064 1872 Size: 1192 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2318 2633 3841 4742 5004 820 Size: 197 926 375 315 1208 901 262 1553 Fragments arranged by size: 1553 1208 926 901 375 315 262 197 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 2678 2752 3095 1132 Size: 1546 74 343 3774 Fragments arranged by size: 3774 1546 343 74 SspI AAT'ATT Cuts at: 5 52 3661 5 Size: 47 3609 2081 Fragments arranged by size: 3609 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5737 StyI C'CwwG_G Cuts at: 513 1065 2004 2578 2978 3176 513 Size: 552 939 574 400 198 3074 Fragments arranged by size: 3074 939 574 552 400 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3764 3781 3934 4119 4564 325 Size: 1255 2184 17 153 185 445 1498 Fragments arranged by size: 2184 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 2571 3983 Size: 274 80 33 51 1443 300 294 1412 Cuts at: 3983 5695 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1412 300 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2759 2791 5697 5729 2759 Size: 32 2906 32 2767 Fragments arranged by size: 2906 2767 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5737 XbaI T'CTAG_A Cuts at: 2464 3073 2464 Size: 609 5128 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5737 XmaI C'CCGG_G Cuts at: 1870 3062 1870 Size: 1192 4545 XmnI GAAnn'nnTTC Cuts at: 3866 3866 Size: 5737 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI