Restriction map for
pFN21AE0773
(Linear) (Six-base) MAPSORT of: KIEE0773.seq Check: 7459 from: 1 to: 1032
KIEE0773.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 401 1032
Size: 401 631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1032
Size: 661 371
AflIII A'CryG_T
Cuts at: 0 269 1032
Size: 269 763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 1032
Size: 796 236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 856 888 1032
Size: 856 32 144
Fragments arranged by size:
856 144 32
AvaI C'yCGr_G
Cuts at: 0 281 1032
Size: 281 751
BanI G'GyrC_C
Cuts at: 0 703 1032
Size: 703 329
BanII G_rGCy'C
Cuts at: 0 485 629 900 1032
Size: 485 144 271 132
Fragments arranged by size:
485 271 144 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 697 1032
Size: 697 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 391 1032
Size: 357 34 641
Fragments arranged by size:
641 357 34
Bme1580I G_kGCm'C
Cuts at: 0 890 1032
Size: 890 142
Bpu10I CC'TnA_GC
Cuts at: 0 61 922 1032
Size: 61 861 110
Fragments arranged by size:
861 110 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 613 1032
Size: 298 315 419
Fragments arranged by size:
419 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 0 832 966 1032
Size: 832 134 66
Fragments arranged by size:
832 134 66
BsaBI GATnn'nnATC
Cuts at: 0 379 1032
Size: 379 653
BsaHI Gr'CG_yC
Cuts at: 0 240 1032
Size: 240 792
BsaWI w'CCGG_w
Cuts at: 0 86 437 1032
Size: 86 351 595
Fragments arranged by size:
595 351 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1032
Size: 895 30 107
Fragments arranged by size:
895 107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 559 699 1032
Size: 456 103 140 333
Fragments arranged by size:
456 333 140 103
BseYI C'CCAG_C
Cuts at: 0 74 1032
Size: 74 958
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 379 492 1032
Size: 95 284 113 540
Fragments arranged by size:
540 284 113 95
BsiEI CG_ry'CG
Cuts at: 0 39 1032
Size: 39 993
BsiHKAI G_wGCw'C
Cuts at: 0 67 485 1032
Size: 67 418 547
Fragments arranged by size:
547 418 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 421 1032
Size: 421 611
Bsp1286I G_dGCh'C
Cuts at: 0 67 485 629 890 900 1032
Size: 67 418 144 261 10 132
Fragments arranged by size:
418 261 144 132 67 10
BspEI T'CCGG_A
Cuts at: 0 86 1032
Size: 86 946
BspHI T'CATG_A
Cuts at: 0 122 1032
Size: 122 910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 725 1032
Size: 401 324 307
Fragments arranged by size:
401 324 307
BsrBI CCG'CTC
Cuts at: 0 131 1032
Size: 131 901
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 1032
Size: 363 669
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 1032
Size: 540 492
BstYI r'GATC_y
Cuts at: 0 446 762 1032
Size: 446 316 270
Fragments arranged by size:
446 316 270
Bsu36I CC'TnA_GG
Cuts at: 0 682 1032
Size: 682 350
BtgI C'CryG_G
Cuts at: 0 474 573 890 1032
Size: 474 99 317 142
Fragments arranged by size:
474 317 142 99
BtsI GCAGTG_nn'
Cuts at: 0 189 1032
Size: 189 843
EaeI y'GGCC_r
Cuts at: 0 36 159 1032
Size: 36 123 873
Fragments arranged by size:
873 123 36
EagI C'GGCC_G
Cuts at: 0 36 1032
Size: 36 996
EarI CTCTTCn'nnn_
Cuts at: 0 328 496 1032
Size: 328 168 536
Fragments arranged by size:
536 328 168
EcoICRI GAG'CTC
Cuts at: 0 483 1032
Size: 483 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1032
Size: 661 371
EcoNI CCTnn'n_nnAGG
Cuts at: 0 681 955 1032
Size: 681 274 77
Fragments arranged by size:
681 274 77
EcoO109I rG'GnC_Cy
Cuts at: 0 318 903 1032
Size: 318 585 129
Fragments arranged by size:
585 318 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 339 1032
Size: 339 693
FspI TGC'GCA
Cuts at: 0 367 1032
Size: 367 665
HaeII r_GCGC'y
Cuts at: 0 313 1032
Size: 313 719
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 811 843 895 927 1032
Size: 225 32 554 32 52 32 105
Fragments arranged by size:
554 225 105 52 32 32 32
MfeI C'AATT_G
Cuts at: 0 218 1032
Size: 218 814
MscI TGG'CCA
Cuts at: 0 161 1032
Size: 161 871
MslI CAynn'nnrTG
Cuts at: 0 538 778 1032
Size: 538 240 254
Fragments arranged by size:
538 254 240
MspA1I CmG'CkG
Cuts at: 0 151 363 476 1032
Size: 151 212 113 556
Fragments arranged by size:
556 212 151 113
NcoI C'CATG_G
Cuts at: 0 573 1032
Size: 573 459
NspI r_CATG'y
Cuts at: 0 673 747 1032
Size: 673 74 285
Fragments arranged by size:
673 285 74
PpuMI rG'GwC_Cy
Cuts at: 0 318 903 1032
Size: 318 585 129
Fragments arranged by size:
585 318 129
PstI C_TGCA'G
Cuts at: 0 500 1032
Size: 500 532
PvuII CAG'CTG
Cuts at: 0 151 363 1032
Size: 151 212 669
Fragments arranged by size:
669 212 151
SacI G_AGCT'C
Cuts at: 0 485 1032
Size: 485 547
SacII CC_GC'GG
Cuts at: 0 477 1032
Size: 477 555
SapI GCTCTTCn'nnn_
Cuts at: 0 496 1032
Size: 496 536
SfcI C'TryA_G
Cuts at: 0 496 1032
Size: 496 536
SmlI C'TyrA_G
Cuts at: 0 313 628 1032
Size: 313 315 404
Fragments arranged by size:
404 315 313
SphI G_CATG'C
Cuts at: 0 673 747 1032
Size: 673 74 285
Fragments arranged by size:
673 285 74
StyI C'CwwG_G
Cuts at: 0 573 973 1032
Size: 573 400 59
Fragments arranged by size:
573 400 59
TatI w'GTAC_w
Cuts at: 0 272 566 1032
Size: 272 294 466
Fragments arranged by size:
466 294 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 754 786 1032
Size: 754 32 246
Fragments arranged by size:
754 246 32
XbaI T'CTAG_A
Cuts at: 0 459 1032
Size: 459 573
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AvaI BanI BanII
BbsI BcgI Bme1580I Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I MfeI MscI
MslI MspA1I NcoI NspI PpuMI PstI PvuII SacI
SacII SapI SfcI SmlI SphI StyI TatI TstI
XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI
BsaAI BsiWI BsmI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII Tth111I XcmI XhoI
XmaI XmnI ZraI