Restriction map for
pFN21AB7813
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I AlwI AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HpaI
KasI KpnI KspI MamI MflI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 361 GACGTC GACGT^C
AccI 1190 GTCGAC GT^[AC][GT]AC
AciI 1036 CCGC CCGC(-3/-1)
AcsI 159 AAATTC [GA]^AATT[CT]
AflIII 49 ACGTGT A^C[GA][CT]GT
ApaI 385 GGGCCC GGGCC^C
ApoI 159 AAATTC [GA]^AATT[CT]
Asp700I 513 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1462 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 789 GGTGCC G^G[CT][GA]CC
BbsI 1396 GTCTTC GAAGAC(2/6)
BbvCI 1006 GCTGAGG CCTCAGC(-5/-2)
BciVI 1350 GGATAC GTATCC(6/5)
BcnI 1503 CCGGG CC^[GC]GG
BpuAI 1396 GTCTTC GAAGAC(2/6)
BsaI 801 GAGACC GGTCTC(1/5)
BseRI 652 GAGGAG GAGGAG(10/8)
BsmI 1516 GAATGC GAATGC(1/-1)
Bsp1407I 1472 TGTACA T^GTACA
BsrDI 4 CATTGC GCAATG(2/0)
BsrGI 1472 TGTACA T^GTACA
BstXI 1478 CCATTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 129 GCATGC GC[ACGT]^[ACGT]GC
CfoI 639 GCGC GCG^C
DpnII 228 GATC ^GATC
DrdI 1181 GACCAAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1494 CCATGG C^C[GA][CT]GG
EarI 190 GAAGAG CTCTTC(1/4)
EcoT22I 1 ATGCAT ATGCA^T
FauI 1035 CCCGC CCCGC(4/6)
HgaI 559 GACGC GACGC(5/10)
HhaI 639 GCGC GCG^C
HinP1I 639 GCGC G^CGC
HincII 1190 GTCGAC GT[CT]^[GA]AC
HindII 1190 GTCGAC GT[CT]^[GA]AC
HindIII 1343 AAGCTT A^AGCTT
Ksp632I 190 GAAGAG CTCTTC(1/4)
MboI 228 GATC ^GATC
MfeI 95 CAATTG C^AATTG
MunI 95 CAATTG C^AATTG
NciI 1503 CCGGG CC^[GC]GG
NcoI 1494 CCATGG C^CATGG
NdeII 228 GATC ^GATC
NsiI 1 ATGCAT ATGCA^T
NspI 129 GCATGC [GA]CATG^[CT]
PflFI 1462 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 585 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 1190 GTCGAC G^TCGAC
Sau3AI 228 GATC ^GATC
SmlI 108 CTCAAG C^T[CT][GA]AG
SphI 129 GCATGC GCATG^C
SspBI 1472 TGTACA T^GTACA
SspI 778 AATATT AAT^ATT
StuI 1208 AGGCCT AGG^CCT
Tth111I 1462 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 585 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 389 CCAATGTGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 513 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 361 GACGTC G[GA]^CG[CT]C
559 GACGCC
AhdI 803 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1264 GACCACCAGTC
AocI 576 CCTCAGG CC^T[ACGT]AGG
1366 CCTAAGG
AspEI 803 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1264 GACCACCAGTC
BanII 385 GGGCCC G[GA]GC[CT]^C
1371 GGGCTC
BlpI 943 GCTGAGC GC^T[ACGT]AGC
1073 GCTCAGC
Bpu1102I 943 GCTGAGC GC^T[ACGT]AGC
1073 GCTCAGC
BsaHI 361 GACGTC G[GA]^CG[CT]C
559 GACGCC
BsmFI 557 GGGAC GGGAC(10/14)
1505 GGGAC
BspHI 716 TCATGA T^CATGA
1143 TCATGA
BspMI 804 ACCTGC ACCTGC(4/8)
711 GCAGGT
BstAPI 371 GCATGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1220 GCAATAGCTGC
Bsu36I 576 CCTCAGG CC^T[ACGT]AGG
1366 CCTAAGG
CelII 943 GCTGAGC GC^T[ACGT]AGC
1073 GCTCAGC
Eam1105I 803 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1264 GACCACCAGTC
Eco81I 576 CCTCAGG CC^T[ACGT]AGG
1366 CCTAAGG
HapII 333 CCGG C^CGG
1503 CCGG
Hin1I 361 GACGTC G[GA]^CG[CT]C
559 GACGCC
HpaII 333 CCGG C^CGG
1503 CCGG
MseI 925 TTAA T^TAA
1294 TTAA
MspI 333 CCGG C^CGG
1503 CCGG
NdeI 929 CATATG CA^TATG
961 CATATG
PpuMI 580 AGGACCC [GA]G^G[AT]CC[CT]
1505 GGGACCC
RcaI 716 TCATGA T^CATGA
1143 TCATGA
SfaNI 951 GATGC GCATC(5/9)
1026 GATGC
TaqI 1191 TCGA T^CGA
1575 TCGA
Tru9I 925 TTAA T^TAA
1294 TTAA
TthHB8I 1191 TCGA T^CGA
1575 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AfaI AluI AspHI AvaII BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboII MnlI
MslI MvaI MwoI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfcI
StyI TfiI TseI Tsp45I Tsp509I
TspRI