Restriction map for pFN21AB7813
 (Linear) (Six-base) MAPSORT of: KIBB7813.seq  Check: 7822  from: 1  to: 1596



KIBB7813.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AatII G_ACGT'C

Cuts at:      0     365    1596 
   Size:        365    1231
 
AccI GT'mk_AC

Cuts at:      0    1191    1596 
   Size:       1191     405
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1073    1399    1596 
   Size:       1073     326     197

  Fragments arranged by size: 

                1073     326     197
 
AflIII A'CryG_T

Cuts at:      0      49    1596 
   Size:         49    1547
 
AhdI GACnn_n'nnGTC

Cuts at:      0     808    1269    1596 
   Size:        808     461     327

  Fragments arranged by size: 

                 808     461     327
 
AleI CACnn'nnGTG

Cuts at:      0     422    1596 
   Size:        422    1174
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     419     451    1596 
   Size:        419      32    1145

  Fragments arranged by size: 

                1145     419      32
 
ApaI G_GGCC'C

Cuts at:      0     389    1596 
   Size:        389    1207
 
ApoI r'AATT_y

Cuts at:      0     159    1596 
   Size:        159    1437
 
BanI G'GyrC_C

Cuts at:      0     789    1596 
   Size:        789     807
 
BanII G_rGCy'C

Cuts at:      0     389    1375    1596 
   Size:        389     986     221

  Fragments arranged by size: 

                 986     389     221
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1389    1596 
   Size:       1389     207
 
BbvCI CC'TCA_GC

Cuts at:      0    1007    1596 
   Size:       1007     589
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1344    1596 
   Size:       1344     252
 
BfrBI ATG'CAT

Cuts at:      0       3    1596 
   Size:          3    1593
 
BlpI GC'TnA_GC

Cuts at:      0     944    1074    1596 
   Size:        944     130     522

  Fragments arranged by size: 

                 944     522     130
 
Bme1580I G_kGCm'C

Cuts at:      0     389    1596 
   Size:        389    1207
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1057    1596 
   Size:       1057     539
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     667     670    1596 
   Size:        527     140       3     926

  Fragments arranged by size: 

                 926     527     140       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     211    1007    1596 
   Size:        211     796     589

  Fragments arranged by size: 

                 796     589     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93    1596 
   Size:         93    1503
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     795    1596 
   Size:        795     801
 
BsaHI Gr'CG_yC

Cuts at:      0     362     560    1596 
   Size:        362     198    1036

  Fragments arranged by size: 

                1036     362     198
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1347    1377    1596 
   Size:       1347      30     219

  Fragments arranged by size: 

                1347     219      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     667    1596 
   Size:        667     929
 
BseYI C'CCAG_C

Cuts at:      0     706    1216    1317    1596 
   Size:        706     510     101     279

  Fragments arranged by size: 

                 706     510     279     101
 
BsiHKAI G_wGCw'C

Cuts at:      0     659    1442    1586    1596 
   Size:        659     783     144      10

  Fragments arranged by size: 

                 783     659     144      10
 
BsmI GAATG_Cn'

Cuts at:      0    1522    1596 
   Size:       1522      74
 
Bsp1286I G_dGCh'C

Cuts at:      0     389     659    1375    1442    1586    1596 
   Size:        389     270     716      67     144      10

  Fragments arranged by size: 

                 716     389     270     144      67      10
 
BspHI T'CATG_A

Cuts at:      0     716    1143    1596 
   Size:        716     427     453

  Fragments arranged by size: 

                 716     453     427
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     702     813    1596 
   Size:        702     111     783

  Fragments arranged by size: 

                 783     702     111
 
BsrDI GCAATG_nn'

Cuts at:      0       3    1596 
   Size:          3    1593
 
BsrGI T'GTAC_A

Cuts at:      0    1472    1596 
   Size:       1472     124
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     377    1226    1596 
   Size:        377     849     370

  Fragments arranged by size: 

                 849     377     370
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1485    1596 
   Size:       1485     111
 
Bsu36I CC'TnA_GG

Cuts at:      0     577    1367    1596 
   Size:        577     790     229

  Fragments arranged by size: 

                 790     577     229
 
BtgI C'CryG_G

Cuts at:      0    1494    1596 
   Size:       1494     102
 
BtsI GCAGTG_nn'

Cuts at:      0    1440    1596 
   Size:       1440     156
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     600     635    1509    1544    1596 
   Size:        600      35     874      35      52

  Fragments arranged by size: 

                 874     600      52      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1187    1596 
   Size:       1187     409
 
EarI CTCTTCn'nnn_

Cuts at:      0     185    1596 
   Size:        185    1411
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     667     670    1073    1399    1596 
   Size:        527     140       3     403     326     197

  Fragments arranged by size: 

                 527     403     326     197     140       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     581    1011    1424    1506    1596 
   Size:        581     430     413      82      90

  Fragments arranged by size: 

                 581     430     413      90      82
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1094    1337    1596 
   Size:       1094     243     259

  Fragments arranged by size: 

                1094     259     243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     794     826     942     974    1255    1287 
   Size:         91      32     671      32     116      32     281      32

Cuts at:   1287    1546    1578    1596 
   Size:        259      32      18

  Fragments arranged by size: 

                 671     281     259     116      91      32      32      32
                  32      32      18
 
HincII GTy'rAC

Cuts at:      0    1192    1596 
   Size:       1192     404
 
HindIII A'AGCT_T

Cuts at:      0    1343    1596 
   Size:       1343     253
 
MfeI C'AATT_G

Cuts at:      0      95    1596 
   Size:         95    1501
 
MslI CAynn'nnrTG

Cuts at:      0     422    1483    1515    1596 
   Size:        422    1061      32      81

  Fragments arranged by size: 

                1061     422      81      32
 
NcoI C'CATG_G

Cuts at:      0    1494    1596 
   Size:       1494     102
 
NdeI CA'TA_TG

Cuts at:      0     930     962    1596 
   Size:        930      32     634

  Fragments arranged by size: 

                 930     634      32
 
NsiI A_TGCA'T

Cuts at:      0       5    1596 
   Size:          5    1591
 
NspI r_CATG'y

Cuts at:      0     133    1596 
   Size:        133    1463
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     591    1596 
   Size:        591    1005
 
PfoI T'CCnGG_A

Cuts at:      0     305    1596 
   Size:        305    1291
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     502     534    1596 
   Size:        502      32    1062

  Fragments arranged by size: 

                1062     502      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     581    1506    1596 
   Size:        581     925      90

  Fragments arranged by size: 

                 925     581      90
 
PsiI TTA'TAA

Cuts at:      0    1069    1596 
   Size:       1069     527
 
PspOMI G'GGCC_C

Cuts at:      0     385    1596 
   Size:        385    1211
 
SalI G'TCGA_C

Cuts at:      0    1190    1596 
   Size:       1190     406
 
SanDI GG'GwC_CC

Cuts at:      0    1506    1596 
   Size:       1506      90
 
SfcI C'TryA_G

Cuts at:      0     534     546    1533    1596 
   Size:        534      12     987      63

  Fragments arranged by size: 

                 987     534      63      12
 
SmlI C'TyrA_G

Cuts at:      0     108    1596 
   Size:        108    1488
 
SphI G_CATG'C

Cuts at:      0     133    1596 
   Size:        133    1463
 
SspI AAT'ATT

Cuts at:      0     780    1596 
   Size:        780     816
 
StuI AGG'CCT

Cuts at:      0    1210    1596 
   Size:       1210     386
 
StyI C'CwwG_G

Cuts at:      0     999    1278    1358    1494    1596 
   Size:        999     279      80     136     102

  Fragments arranged by size: 

                 999     279     136     102      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     132    1596 
   Size:        132    1464
 
TatI w'GTAC_w

Cuts at:      0    1472    1596 
   Size:       1472     124
 
Tth111I GACn'n_nGTC

Cuts at:      0    1465    1596 
   Size:       1465     131
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     396    1596 
   Size:        396    1200
 
XmnI GAAnn'nnTTC

Cuts at:      0     517    1596 
   Size:        517    1079
 
ZraI GAC'GTC

Cuts at:      0     363    1596 
   Size:        363    1233
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AhdI     AleI     AloI     ApaI
     ApoI     BanI    BanII     BbsI    BbvCI    BciVI    BfrBI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI   BstAPI    BstXI   Bsu36I     BtgI     BtsI    CspCI     DrdI
     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII  HindIII     MfeI
     MslI     NcoI     NdeI     NsiI     NspI    PflMI     PfoI     PpiI
    PpuMI     PsiI   PspOMI     SalI    SanDI     SfcI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI  Tth111I     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI    AlwNI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BcgI     BclI     BglI    BglII    BmgBI     BmtI     BplI
    BsaAI    BsaBI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I
    BtgZI     ClaI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI    PshAI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     TstI
     XbaI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com