Restriction map for
pFN21AB7627
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AlwI AlwNI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI Sau3AI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
SspBI SspI SwaI Tsp509I Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AhdI 38 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 318 GTGCAC G^TGCAC
AocI 400 CCTCAGG CC^T[ACGT]AGG
ApaLI 318 GTGCAC G^TGCAC
AspEI 38 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 652 CTCGAG C^[CT]CG[GA]G
BseRI 134 CTCCTC GAGGAG(10/8)
BsmI 757 GAATGC GAATGC(1/-1)
BsoBI 652 CTCGAG C^[CT]CG[GA]G
BsrDI 505 CATTGC GCAATG(2/0)
BstF5I 312 CATCC GGATG(2/0)
BstXI 54 CCAACGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 400 CCTCAGG CC^T[ACGT]AGG
DsaI 704 CCATGG C^C[GA][CT]GG
Eam1105I 38 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 400 CCTCAGG CC^T[ACGT]AGG
FokI 312 CATCC GGATG(9/13)
HincII 217 GTCAAC GT[CT]^[GA]AC
HindII 217 GTCAAC GT[CT]^[GA]AC
MaeIII 46 GTCAC ^GT[ACGT]AC
MseI 641 TTAA T^TAA
MspA1I 153 CAGCTG C[AC]G^C[GT]G
NcoI 704 CCATGG C^CATGG
NlaIII 705 CATG CATG^
PaeR7I 652 CTCGAG C^TCGAG
PvuII 153 CAGCTG CAG^CTG
SacI 859 GAGCTC GAGCT^C
SapI 468 GCTCTTC GCTCTTC(1/4)
SbfI 294 CCTGCAGG CCTGCA^GG
ScaI 20 AGTACT AGT^ACT
SmlI 652 CTCGAG C^T[CT][GA]AG
SpeI 673 ACTAGT A^CTAGT
Sse8387I 294 CCTGCAGG CCTGCA^GG
StuI 291 AGGCCT AGG^CCT
TfiI 559 GAATC G^A[AT]TC
Tru9I 641 TTAA T^TAA
Tsp45I 46 GTCAC ^GT[GC]AC
XhoI 652 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 21 GTAC GT^AC
144 GTAC
AspHI 318 GTGCAC G[AT]GC[AT]^C
859 GAGCTC
BanII 466 GGGCTC G[GA]GC[CT]^C
859 GAGCTC
BfaI 439 CTAG C^TAG
674 CTAG
BsaI 589 GAGACC GGTCTC(1/5)
814 GAGACC
BsiHKAI 318 GTGCAC G[AT]GC[AT]^C
859 GAGCTC
BstAPI 122 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
311 GCATCCCGTGC
EarI 469 CTCTTC CTCTTC(1/4)
565 CTCTTC
Ksp632I 469 CTCTTC CTCTTC(1/4)
565 CTCTTC
MaeI 439 CTAG C^TAG
674 CTAG
PleI 601 GAGTC GAGTC(4/5)
694 GAGTC
RsaI 21 GTAC GT^AC
144 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AvaII BbvI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI MboII MnlI MvaI MwoI
NlaIV PstI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI TspRI
TthHB8I