Restriction map for
pFN21AB0494
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BcgI BclI BfrI
BlnI BsaWI BseAI BsiWI BspEI
BspHI BspLU11I BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I DraI
DraIII Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MfeI
MluI MroI MseI MunI NdeI
NheI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tru9I Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 1366 GTAGAC GT^[AC][GT]AC
AcsI 896 AAATTT [GA]^AATT[CT]
Alw44I 2044 GTGCAC G^TGCAC
ApaLI 2044 GTGCAC G^TGCAC
ApoI 896 AAATTT [GA]^AATT[CT]
BamHI 2712 GGATCC G^GATCC
BbrPI 736 CACGTG CAC^GTG
BciVI 1384 GGATAC GTATCC(6/5)
BglII 2672 AGATCT A^GATCT
BsaBI 2187 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 238 GAGACG CGTCTC(1/5)
BsmI 963 GCATTC GAATGC(1/-1)
Bsp1407I 1433 TGTACA T^GTACA
BspDI 1372 ATCGAT AT^CGAT
BsrGI 1433 TGTACA T^GTACA
BssHII 2779 GCGCGC G^CGCGC
ClaI 1372 ATCGAT AT^CGAT
CpoI 2315 CGGTCCG CG^G[AT]CCG
DrdI 2308 GACGTGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 504 CGGCCG C^GGCCG
EclXI 504 CGGCCG C^GGCCG
Eco52I 504 CGGCCG C^GGCCG
KspI 211 CCGCGG CCGC^GG
MamI 2187 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1646 GCCGGC GCC^GGC
NgoMIV 1646 GCCGGC G^CCGGC
NotI 503 GCGGCCGC GC^GGCCGC
NruI 2195 TCGCGA TCG^CGA
NspI 2069 ACATGC [GA]CATG^[CT]
PmaCI 736 CACGTG CAC^GTG
PmlI 736 CACGTG CAC^GTG
RsrII 2315 CGGTCCG CG^G[AT]CCG
SacII 211 CCGCGG CCGC^GG
SapI 1503 GAAGAGC GCTCTTC(1/4)
SbfI 2766 CCTGCAGG CCTGCA^GG
SexAI 1421 ACCAGGT A^CC[AT]GGT
Sse8387I 2766 CCTGCAGG CCTGCA^GG
SspBI 1433 TGTACA T^GTACA
Tsp509I 897 AATT ^AATT
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 397 GACGTC GACGT^C
982 GACGTC
AflIII 737 ACGTGT A^C[GA][CT]GT
2885 ACGTGT
ApaI 222 GGGCCC GGGCC^C
2075 GGGCCC
BbsI 462 GAAGAC GAAGAC(2/6)
2480 GTCTTC
BbvCI 2487 CCTCAGC CCTCAGC(-5/-2)
1287 GCTGAGG
BfaI 439 CTAG C^TAG
1963 CTAG
BglI 131 GCCCCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2697 GCCCTCGAGGC
BpuAI 462 GAAGAC GAAGAC(2/6)
2480 GTCTTC
BsaAI 556 TACGTG [CT]AC^GT[GA]
736 CACGTG
BsrDI 1212 CATTGC GCAATG(2/0)
2084 GCAATG
EcoNI 675 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2049 CCTGCCCCAGG
HincII 985 GTCAAC GT[CT]^[GA]AC
2587 GTCAAC
HindII 985 GTCAAC GT[CT]^[GA]AC
2587 GTCAAC
MaeI 439 CTAG C^TAG
1963 CTAG
PaeR7I 705 CTCGAG C^TCGAG
2700 CTCGAG
SacI 1439 GAGCTC GAGCT^C
2446 GAGCTC
ScaI 518 AGTACT AGT^ACT
779 AGTACT
StuI 1457 AGGCCT AGG^CCT
2842 AGGCCT
XhoI 705 CTCGAG C^TCGAG
2700 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII