(Circular) (Six-base) MAPSORT of: pFN21AB0494.seq Check: 3118 from: 1 to: 7822 pFN21AB0494 7822 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4062 4062 Size: 7822 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2406 2991 5632 278 Size: 53 83 186 936 870 585 2641 2468 Fragments arranged by size: 2641 2468 936 870 585 186 83 53 AccI GT'mk_AC Cuts at: 1937 3372 5165 1937 Size: 1435 1793 4594 Fragments arranged by size: 4594 1793 1435 Acc65I G'GTAC_C Cuts at: 5143 5143 Size: 7822 AclI AA'CG_TT Cuts at: 1563 5949 6322 1563 Size: 4386 373 3063 Fragments arranged by size: 4386 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2593 3917 4553 4697 5885 7269 7537 1632 Size: 961 1324 636 144 1188 1384 268 1917 Fragments arranged by size: 1917 1384 1324 1188 961 636 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7625 AflIII A'CryG_T Cuts at: 1203 2742 4890 6721 7698 1203 Size: 1539 2148 1831 977 1327 Fragments arranged by size: 2148 1831 1539 1327 977 AgeI A'CCGG_T Cuts at: 6653 6653 Size: 7822 AhdI GACnn_n'nnGTC Cuts at: 6551 6551 Size: 7822 AleI CACnn'nnGTG Cuts at: 1166 3869 1166 Size: 2703 5119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4319 1184 Size: 3135 4687 AlwNI CAG_nnn'CTG Cuts at: 2926 2978 3227 3316 3341 3478 5565 7137 2926 Size: 52 249 89 25 137 2087 1572 3611 Fragments arranged by size: 3611 2087 1572 249 137 89 52 25 ApaI G_GGCC'C Cuts at: 2231 4084 2231 Size: 1853 5969 ApaLI G'TGCA_C Cuts at: 4049 5879 7035 4049 Size: 1830 1156 4836 Fragments arranged by size: 4836 1830 1156 ApoI r'AATT_y Cuts at: 1493 2901 5131 5357 5509 7618 1493 Size: 1408 2230 226 152 2109 1697 Fragments arranged by size: 2230 2109 1697 1408 226 152 AseI AT'TA_AT Cuts at: 160 1032 6376 160 Size: 872 5344 1606 Fragments arranged by size: 5344 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7822 AvaI C'yCGr_G Cuts at: 1870 1943 2710 4694 4705 4932 5147 1870 Size: 73 767 1984 11 227 215 4545 Fragments arranged by size: 4545 1984 767 227 215 73 11 BamHI G'GATC_C Cuts at: 4717 5152 5519 4717 Size: 435 367 7020 Fragments arranged by size: 7020 435 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2210 2864 3119 3365 3686 Size: 325 217 627 423 654 255 246 321 Cuts at: 3686 4064 4744 4912 5143 6598 618 Size: 378 680 168 231 1455 1842 Fragments arranged by size: 1842 1455 680 654 627 423 378 325 321 255 246 231 217 168 BanII G_rGCy'C Cuts at: 1391 2231 2653 3448 3674 3788 3959 4084 4455 Size: 840 422 795 226 114 171 125 371 Cuts at: 4455 4695 5141 1391 Size: 240 446 4072 Fragments arranged by size: 4072 840 795 446 422 371 240 226 171 125 114 BbeI G_GCGC'C Cuts at: 2214 4068 4748 2214 Size: 1854 680 5288 Fragments arranged by size: 5288 1854 680 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2474 4478 928 Size: 971 575 2004 4272 Fragments arranged by size: 4272 2004 971 575 BbvCI CC'TCA_GC Cuts at: 3293 4493 3293 Size: 1200 6622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6013 6047 1903 Size: 34 4076 34 3678 Fragments arranged by size: 4076 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3383 5714 6924 3383 Size: 2331 1210 4281 Fragments arranged by size: 4281 2331 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7822 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 4708 6433 Size: 107 122 71 631 605 470 2566 1725 Cuts at: 6433 136 Size: 1525 Fragments arranged by size: 2566 1725 1525 631 605 470 122 107 71 BglII A'GATC_T Cuts at: 4677 7817 4677 Size: 3140 4682 BlpI GC'TnA_GC Cuts at: 2496 3447 3782 3797 4922 5239 2496 Size: 951 335 15 1125 317 5079 Fragments arranged by size: 5079 1125 951 335 317 15 Bme1580I G_kGCm'C Cuts at: 1790 2231 2267 2294 2869 3124 3585 3927 4053 Size: 441 36 27 575 255 461 342 126 Cuts at: 4053 4084 4917 5883 7039 1790 Size: 31 833 966 1156 2573 Fragments arranged by size: 2573 1156 966 833 575 461 441 342 255 126 36 31 27 BmgBI CAC'GTC Cuts at: 1318 3302 4315 1318 Size: 1984 1013 4825 Fragments arranged by size: 4825 1984 1013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2105 2384 2809 Size: 416 53 83 399 24 688 279 425 Cuts at: 2809 3240 3588 4621 5553 6511 442 Size: 431 348 1033 932 958 1753 Fragments arranged by size: 1753 1033 958 932 688 431 425 416 399 348 279 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7822 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4720 4720 Size: 7822 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2070 2290 2416 2676 2755 4010 6482 Size: 827 4 220 126 260 79 1255 2472 Cuts at: 6482 1239 Size: 2579 Fragments arranged by size: 2579 2472 1255 827 260 220 126 79 4 Bpu10I CC'TnA_GC Cuts at: 2549 3293 4085 4493 4944 2549 Size: 744 792 408 451 5427 Fragments arranged by size: 5427 792 744 451 408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4586 5947 6812 7110 4586 Size: 1361 865 298 5298 Fragments arranged by size: 5298 1361 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 2681 4133 6485 882 Size: 617 816 366 1452 2352 2219 Fragments arranged by size: 2352 2219 1452 816 617 366 BsaAI yAC'GTr Cuts at: 493 1204 2563 2743 5613 493 Size: 711 1359 180 2870 2702 Fragments arranged by size: 2870 2702 1359 711 180 BsaBI GATnn'nnATC Cuts at: 4196 7816 4196 Size: 3620 4202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 2381 2403 2471 Size: 53 83 186 936 678 170 22 68 Cuts at: 2471 2954 2988 4065 4745 5629 6011 6646 275 Size: 483 34 1077 680 884 382 635 1451 Fragments arranged by size: 1451 1077 936 884 680 678 635 483 382 186 170 83 68 53 34 22 BsaWI w'CCGG_w Cuts at: 6255 6653 6927 7074 7485 6255 Size: 398 274 147 411 6592 Fragments arranged by size: 6592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2625 2655 2732 2762 4232 Size: 30 165 30 1222 30 77 30 1470 Cuts at: 4232 4262 4880 4910 4947 4977 1178 Size: 30 618 30 37 30 4023 Fragments arranged by size: 4023 1470 1222 618 165 77 37 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2471 4991 1186 Size: 1069 216 2520 4017 Fragments arranged by size: 4017 2520 1069 216 BseYI C'CCAG_C Cuts at: 2231 2580 3115 3125 3667 3927 4162 4235 4422 Size: 349 535 10 542 260 235 73 187 Cuts at: 4422 4518 4628 4832 5017 7025 7521 2231 Size: 96 110 204 185 2008 496 2532 Fragments arranged by size: 2532 2008 542 535 496 349 260 235 204 187 185 110 96 73 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 3893 4781 1163 Size: 573 399 1758 888 4204 Fragments arranged by size: 4204 1758 888 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2388 2512 3199 5272 6033 7061 Size: 275 186 387 124 687 2073 761 1028 Cuts at: 7061 1540 Size: 2301 Fragments arranged by size: 2301 2073 1028 761 687 387 275 186 124 BsiHKAI G_wGCw'C Cuts at: 3448 3798 4053 4443 4455 5883 5968 7039 3448 Size: 350 255 390 12 1428 85 1071 4231 Fragments arranged by size: 4231 1428 1071 390 350 255 85 12 BsmI GAATG_Cn' Cuts at: 2968 5333 5426 2968 Size: 2365 93 5364 Fragments arranged by size: 5364 2365 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2237 2237 Size: 7822 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 2267 2294 2653 2869 3124 3448 Size: 399 441 36 27 359 216 255 324 Cuts at: 3448 3585 3674 3788 3798 3927 3959 4053 4084 Size: 137 89 114 10 129 32 94 31 Cuts at: 4084 4443 4455 4695 4917 5141 5883 5968 7039 Size: 359 12 240 222 224 742 85 1071 Cuts at: 7039 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 441 399 359 359 324 255 240 224 222 216 137 129 114 94 89 85 36 32 31 27 12 10 BspEI T'CCGG_A Cuts at: 7485 7485 Size: 7822 BspHI T'CATG_A Cuts at: 5711 7441 5711 Size: 1730 6092 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4062 4539 4779 5177 844 Size: 3218 477 240 398 3489 Fragments arranged by size: 3489 3218 477 398 240 BsrBI CCG'CTC Cuts at: 1394 1823 2508 2894 3964 4921 5709 1394 Size: 429 685 386 1070 957 788 3507 Fragments arranged by size: 3507 1070 957 788 685 429 386 BsrDI GCAATG_nn' Cuts at: 65 1248 3216 4096 6317 6491 65 Size: 1183 1968 880 2221 174 1396 Fragments arranged by size: 2221 1968 1396 1183 880 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3630 3651 4318 5085 6466 6653 1700 Size: 110 1820 21 667 767 1381 187 2869 Fragments arranged by size: 2869 1820 1381 767 667 187 110 21 BsrGI T'GTAC_A Cuts at: 96 3438 96 Size: 3342 4480 BssHII G'CGCG_C Cuts at: 1925 4784 1925 Size: 2859 4963 BssSI C'ACGA_G Cuts at: 5882 6894 5882 Size: 1012 6810 BstAPI GCAn_nnn'nTGC Cuts at: 794 2935 3013 3227 794 Size: 2141 78 214 5389 Fragments arranged by size: 5389 2141 214 78 BstBI TT'CG_AA Cuts at: 6626 6626 Size: 7822 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7822 BstXI CCAn_nnnn'nTGG Cuts at: 2206 3430 4463 4998 2206 Size: 1224 1033 535 5030 Fragments arranged by size: 5030 1224 1033 535 BstYI r'GATC_y Cuts at: 1972 2836 3703 4677 4717 5152 5519 5904 5921 Size: 864 867 974 40 435 367 385 17 Cuts at: 5921 7373 7459 7471 7817 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 974 867 864 435 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2185 2216 2616 2817 3741 3822 Size: 552 939 181 31 400 201 924 81 Cuts at: 3822 4800 513 Size: 978 3535 Fragments arranged by size: 3535 978 939 924 552 400 201 181 81 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3160 4217 487 Size: 683 56 1934 1057 4092 Fragments arranged by size: 4092 1934 1057 683 56 BtsI GCAGTG_nn' Cuts at: 2024 3228 3939 4769 5338 6132 6152 2024 Size: 1204 711 830 569 794 20 3694 Fragments arranged by size: 3694 1204 830 794 711 569 20 ClaI AT'CG_AT Cuts at: 1554 3378 1554 Size: 1824 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7787 DraI TTT'AAA Cuts at: 5126 5126 Size: 7822 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4319 6829 809 Size: 507 3003 2510 1802 Fragments arranged by size: 3003 2510 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2196 2284 Size: 54 1425 50 275 18 177 189 88 Cuts at: 2284 2509 2814 3063 3250 4044 5052 5087 5269 Size: 225 305 249 187 794 1008 35 182 Cuts at: 5269 6158 7787 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1008 889 794 305 275 249 225 189 187 182 177 88 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2509 5269 1537 Size: 275 697 2760 4090 Fragments arranged by size: 4090 2760 697 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3503 4224 5106 5752 1353 Size: 2150 721 882 646 3423 Fragments arranged by size: 3423 2150 882 721 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3726 6409 6783 6929 387 Size: 1064 138 2137 2683 374 146 1280 Fragments arranged by size: 2683 2137 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3446 4453 3446 Size: 1007 6815 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2070 2290 2416 2593 2676 2755 Size: 393 434 4 220 126 177 83 79 Cuts at: 2755 3917 4010 4553 4697 5885 6482 7269 7537 Size: 1162 93 543 144 1188 597 787 268 Cuts at: 7537 1239 Size: 1524 Fragments arranged by size: 1524 1188 1162 787 597 543 434 393 268 220 177 144 126 93 83 79 4 EcoNI CCTnn'n_nnAGG Cuts at: 2684 4058 2684 Size: 1374 6448 EcoO109I rG'GnC_Cy Cuts at: 2069 2135 2228 3104 3242 3313 4026 4081 4427 Size: 66 93 876 138 71 713 55 346 Cuts at: 4427 4564 2069 Size: 137 5327 Fragments arranged by size: 5327 876 713 346 138 137 93 71 66 55 EcoRI G'AATT_C Cuts at: 5131 5509 5131 Size: 378 7444 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5086 5086 Size: 7822 FspI TGC'GCA Cuts at: 6328 6328 Size: 7822 HaeII r_GCGC'y Cuts at: 1678 2214 3027 4068 4748 6969 1678 Size: 536 813 1041 680 2221 2531 Fragments arranged by size: 2531 2221 1041 813 680 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 2693 2725 2914 Size: 32 135 32 612 32 477 32 189 Cuts at: 2914 2946 2995 3027 3286 3318 4232 4264 6463 Size: 32 49 32 259 32 914 32 2199 Cuts at: 6463 6495 6537 6569 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2199 914 612 477 259 189 135 49 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2992 4594 5166 5412 669 Size: 988 281 1054 1602 572 246 3079 Fragments arranged by size: 3079 1602 1054 988 572 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7822 HpaI GTT'AAC Cuts at: 5412 5412 Size: 7822 KasI G'GCGC_C Cuts at: 2210 4064 4744 2210 Size: 1854 680 5288 Fragments arranged by size: 5288 1854 680 KpnI G_GTAC'C Cuts at: 5147 5147 Size: 7822 MfeI C'AATT_G Cuts at: 5421 5421 Size: 7822 MluI A'CGCG_T Cuts at: 7698 7698 Size: 7822 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3550 4551 6936 7120 1305 Size: 2245 1001 2385 184 2007 Fragments arranged by size: 2385 2245 2007 1001 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2198 5054 10 Size: 54 1425 343 177 189 2856 2778 Fragments arranged by size: 2856 2778 1425 343 189 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2561 3221 3560 3740 3869 4446 Size: 648 1038 357 660 339 180 129 577 Cuts at: 4446 4461 5780 6139 6298 518 Size: 15 1319 359 159 2042 Fragments arranged by size: 2042 1319 1038 660 648 577 359 357 339 180 159 129 15 MspA1I CmG'CkG Cuts at: 1606 2218 2394 3625 4258 4422 4795 4960 5238 Size: 612 176 1231 633 164 373 165 278 Cuts at: 5238 5915 7063 7308 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1231 1148 677 633 612 373 278 245 176 165 164 NaeI GCC'GGC Cuts at: 3653 3653 Size: 7822 NarI GG'CG_CC Cuts at: 2211 4065 4745 2211 Size: 1854 680 5288 Fragments arranged by size: 5288 1854 680 NcoI C'CATG_G Cuts at: 513 1065 2004 2185 3741 4800 513 Size: 552 939 181 1556 1059 3535 Fragments arranged by size: 3535 1556 1059 939 552 181 NdeI CA'TA_TG Cuts at: 387 387 Size: 7822 NgoMIV G'CCGG_C Cuts at: 3651 3651 Size: 7822 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7822 NotI GC'GGCC_GC Cuts at: 2509 5269 2509 Size: 2760 5062 NruI TCG'CGA Cuts at: 4202 4202 Size: 7822 NspI r_CATG'y Cuts at: 1132 1229 4078 5180 6725 1132 Size: 97 2849 1102 1545 2229 Fragments arranged by size: 2849 2229 1545 1102 97 PasI CC'CwG_GG Cuts at: 1348 2224 2887 3184 4060 4469 4558 1348 Size: 876 663 297 876 409 89 4612 Fragments arranged by size: 4612 876 876 663 409 297 89 PciI A'CATG_T Cuts at: 6721 6721 Size: 7822 PflMI CCAn_nnn'nTGG Cuts at: 1108 2374 3115 3265 3316 3478 1108 Size: 1266 741 150 51 162 5452 Fragments arranged by size: 5452 1266 741 162 150 51 PfoI T'CCnGG_A Cuts at: 3315 3771 3894 3987 4507 4542 3315 Size: 456 123 93 520 35 6595 Fragments arranged by size: 6595 520 456 123 93 35 PmeI GTTT'AAAC Cuts at: 5126 5126 Size: 7822 PmlI CAC'GTG Cuts at: 2743 2743 Size: 7822 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5891 5923 7404 7436 5891 Size: 32 1481 32 6277 Fragments arranged by size: 6277 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 3242 3313 4427 2069 Size: 1173 71 1114 5464 Fragments arranged by size: 5464 1173 1114 71 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7822 PsiI TTA'TAA Cuts at: 5392 5392 Size: 7822 PspOMI G'GGCC_C Cuts at: 2227 4080 2227 Size: 1853 5969 PspXI vC'TCGA_Gb Cuts at: 4705 4705 Size: 7822 PstI C_TGCA'G Cuts at: 830 3096 3624 3999 4263 4776 4872 5174 830 Size: 2266 528 375 264 513 96 302 3478 Fragments arranged by size: 3478 2266 528 513 375 302 264 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7822 PvuII CAG'CTG Cuts at: 2394 3625 4258 4422 4960 2394 Size: 1231 633 164 538 5256 Fragments arranged by size: 5256 1231 633 538 164 RsrII CG'GwC_CG Cuts at: 4321 4321 Size: 7822 SacI G_AGCT'C Cuts at: 3448 4455 3448 Size: 1007 6815 SacII CC_GC'GG Cuts at: 2219 2219 Size: 7822 SalI G'TCGA_C Cuts at: 1936 5164 1936 Size: 3228 4594 SanDI GG'GwC_CC Cuts at: 3242 4427 3242 Size: 1185 6637 SapI GCTCTTCn'nnn_ Cuts at: 3503 3503 Size: 7822 SbfI CC_TGCA'GG Cuts at: 4776 5174 4776 Size: 398 7424 ScaI AGT'ACT Cuts at: 2525 2786 6070 7782 2525 Size: 261 3284 1712 2565 Fragments arranged by size: 3284 2565 1712 261 SexAI A'CCwGG_T Cuts at: 3426 3426 Size: 7822 SfcI C'TryA_G Cuts at: 826 1045 2847 3092 3620 3995 4259 4772 4868 Size: 219 1802 245 528 375 264 513 96 Cuts at: 4868 5170 6305 6986 7177 826 Size: 302 1135 681 191 1471 Fragments arranged by size: 1802 1471 1135 681 528 513 375 302 264 245 219 191 96 SfoI GGC'GCC Cuts at: 2212 4066 4746 2212 Size: 1854 680 5288 Fragments arranged by size: 5288 1854 680 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7822 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7822 SmaI CCC'GGG Cuts at: 1872 5149 1872 Size: 3277 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2710 4601 4705 5926 6827 7089 Size: 197 926 767 1891 104 1221 901 262 Cuts at: 7089 820 Size: 1553 Fragments arranged by size: 1891 1553 1221 926 901 767 262 197 104 SnaBI TAC'GTA Cuts at: 493 5613 493 Size: 5120 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7822 SphI G_CATG'C Cuts at: 1132 5180 1132 Size: 4048 3774 SspI AAT'ATT Cuts at: 5 52 5746 5 Size: 47 5694 2081 Fragments arranged by size: 5694 2081 47 StuI AGG'CCT Cuts at: 1517 3464 4849 1517 Size: 1947 1385 4490 Fragments arranged by size: 4490 1947 1385 StyI C'CwwG_G Cuts at: 513 1065 2004 2185 2368 3741 4800 4971 5261 Size: 552 939 181 183 1373 1059 171 290 Cuts at: 5261 513 Size: 3074 Fragments arranged by size: 3074 1373 1059 939 552 290 183 181 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2041 2374 3069 3107 3881 4990 5000 Size: 1255 461 333 695 38 774 1109 10 Cuts at: 5000 5009 5849 5866 6019 6204 6649 325 Size: 9 840 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1109 840 774 695 461 445 333 185 153 38 17 10 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 2784 3438 Size: 274 80 33 51 1443 546 261 654 Cuts at: 3438 4751 6068 7780 96 Size: 1313 1317 1712 138 Fragments arranged by size: 1712 1443 1317 1313 654 546 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2154 2186 2625 2657 2732 2764 3876 3908 4419 Size: 32 439 32 75 32 1112 32 511 Cuts at: 4419 4451 4947 4979 7782 7814 2154 Size: 32 496 32 2803 32 2162 Fragments arranged by size: 2803 2162 1112 511 496 439 75 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7822 XbaI T'CTAG_A Cuts at: 5158 5158 Size: 7822 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3181 3566 3697 5047 3181 Size: 385 131 1350 5956 Fragments arranged by size: 5956 1350 385 131 XhoI C'TCGA_G Cuts at: 1943 2710 4705 1943 Size: 767 1995 5060 Fragments arranged by size: 5060 1995 767 XmaI C'CCGG_G Cuts at: 1870 5147 1870 Size: 3277 4545 XmnI GAAnn'nnTTC Cuts at: 5951 5951 Size: 7822 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2404 2989 5630 276 Size: 53 83 186 936 870 585 2641 2468 Fragments arranged by size: 2641 2468 936 870 585 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NsiI PacI PsrI SfiI SrfI SwaI