Restriction map for pFN21AB0494
 (Linear) (Six-base) MAPSORT of: KIBB0494.seq  Check: 4783  from: 1  to: 3117



KIBB0494.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2057    3117 
   Size:       2057    1060
 
AatII G_ACGT'C

Cuts at:      0     401     986    3117 
   Size:        401     585    2131

  Fragments arranged by size: 

                2131     585     401
 
AccI GT'mk_AC

Cuts at:      0    1367    3117 
   Size:       1367    1750
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588    1912    2548    2692    3117 
   Size:        588    1324     636     144     425

  Fragments arranged by size: 

                1324     636     588     425     144
 
AflIII A'CryG_T

Cuts at:      0     737    2885    3117 
   Size:        737    2148     232

  Fragments arranged by size: 

                2148     737     232
 
AleI CACnn'nnGTG

Cuts at:      0    1864    3117 
   Size:       1864    1253
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2314    3117 
   Size:       2314     803
 
AlwNI CAG_nnn'CTG

Cuts at:      0     921     973    1222    1311    1336    1473    3117 
   Size:        921      52     249      89      25     137    1644

  Fragments arranged by size: 

                1644     921     249     137      89      52      25
 
ApaI G_GGCC'C

Cuts at:      0     226    2079    3117 
   Size:        226    1853    1038

  Fragments arranged by size: 

                1853    1038     226
 
ApaLI G'TGCA_C

Cuts at:      0    2044    3117 
   Size:       2044    1073
 
ApoI r'AATT_y

Cuts at:      0     896    3117 
   Size:        896    2221
 
AvaI C'yCGr_G

Cuts at:      0     705    2689    2700    2927    3117 
   Size:        705    1984      11     227     190

  Fragments arranged by size: 

                1984     705     227     190      11
 
BamHI G'GATC_C

Cuts at:      0    2712    3117 
   Size:       2712     405
 
BanI G'GyrC_C

Cuts at:      0     205     859    1114    1360    1681    2059    2739    2907 
   Size:        205     654     255     246     321     378     680     168

Cuts at:   2907    3117 
   Size:        210

  Fragments arranged by size: 

                 680     654     378     321     255     246     210     205
                 168
 
BanII G_rGCy'C

Cuts at:      0     226     648    1443    1669    1783    1954    2079    2450 
   Size:        226     422     795     226     114     171     125     371

Cuts at:   2450    2690    3117 
   Size:        240     427

  Fragments arranged by size: 

                 795     427     422     371     240     226     226     171
                 125     114
 
BbeI G_GCGC'C

Cuts at:      0     209    2063    2743    3117 
   Size:        209    1854     680     374

  Fragments arranged by size: 

                1854     680     374     209
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     469    2473    3117 
   Size:        469    2004     644

  Fragments arranged by size: 

                2004     644     469
 
BbvCI CC'TCA_GC

Cuts at:      0    1288    2488    3117 
   Size:       1288    1200     629

  Fragments arranged by size: 

                1288    1200     629
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1378    3117 
   Size:       1378    1739
 
BglI GCCn_nnn'nGGC

Cuts at:      0     137    2703    3117 
   Size:        137    2566     414

  Fragments arranged by size: 

                2566     414     137
 
BglII A'GATC_T

Cuts at:      0    2672    3117 
   Size:       2672     445
 
BlpI GC'TnA_GC

Cuts at:      0     491    1442    1777    1792    2917    3117 
   Size:        491     951     335      15    1125     200

  Fragments arranged by size: 

                1125     951     491     335     200      15
 
Bme1580I G_kGCm'C

Cuts at:      0     226     262     289     864    1119    1580    1922    2048 
   Size:        226      36      27     575     255     461     342     126

Cuts at:   2048    2079    2912    3117 
   Size:         31     833     205

  Fragments arranged by size: 

                 833     575     461     342     255     226     205     126
                  36      31      27
 
BmgBI CAC'GTC

Cuts at:      0    1297    2310    3117 
   Size:       1297    1013     807

  Fragments arranged by size: 

                1297    1013     807
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     100     379     804    1235    1583    2616    3117 
   Size:        100     279     425     431     348    1033     501

  Fragments arranged by size: 

                1033     501     431     425     348     279     100
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2715    3117 
   Size:       2715     402
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61      65     285     411     671     750    2005    3117 
   Size:         61       4     220     126     260      79    1255    1112

  Fragments arranged by size: 

                1255    1112     260     220     126      79      61       4
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     544    1288    2080    2488    2939    3117 
   Size:        544     744     792     408     451     178

  Fragments arranged by size: 

                 792     744     544     451     408     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2581    3117 
   Size:       2581     536
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     310     676    2128    3117 
   Size:        310     366    1452     989

  Fragments arranged by size: 

                1452     989     366     310
 
BsaAI yAC'GTr

Cuts at:      0     558     738    3117 
   Size:        558     180    2379

  Fragments arranged by size: 

                2379     558     180
 
BsaBI GATnn'nnATC

Cuts at:      0    2191    3117 
   Size:       2191     926
 
BsaHI Gr'CG_yC

Cuts at:      0     206     376     398     466     949     983    2060    2740 
   Size:        206     170      22      68     483      34    1077     680

Cuts at:   2740    3117 
   Size:        377

  Fragments arranged by size: 

                1077     680     483     377     206     170      68      34
                  22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     620     650     727     757    2227    2257    2875    2905 
   Size:        620      30      77      30    1470      30     618      30

Cuts at:   2905    2942    2972    3117 
   Size:         37      30     145

  Fragments arranged by size: 

                1470     620     618     145      77      37      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     466    2986    3117 
   Size:        250     216    2520     131

  Fragments arranged by size: 

                2520     250     216     131
 
BseYI C'CCAG_C

Cuts at:      0     226     575    1110    1120    1662    1922    2157    2230 
   Size:        226     349     535      10     542     260     235      73

Cuts at:   2230    2417    2513    2623    2827    3012    3117 
   Size:        187      96     110     204     185     105

  Fragments arranged by size: 

                 542     535     349     260     235     226     204     187
                 185     110     105      96      73      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130    1888    2776    3117 
   Size:        130    1758     888     341

  Fragments arranged by size: 

                1758     888     341     130
 
BsiEI CG_ry'CG

Cuts at:      0     383     507    1194    3117 
   Size:        383     124     687    1923

  Fragments arranged by size: 

                1923     687     383     124
 
BsiHKAI G_wGCw'C

Cuts at:      0    1443    1793    2048    2438    2450    3117 
   Size:       1443     350     255     390      12     667

  Fragments arranged by size: 

                1443     667     390     350     255      12
 
BsmI GAATG_Cn'

Cuts at:      0     963    3117 
   Size:        963    2154
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     232    3117 
   Size:        232    2885
 
Bsp1286I G_dGCh'C

Cuts at:      0     226     262     289     648     864    1119    1443    1580 
   Size:        226      36      27     359     216     255     324     137

Cuts at:   1580    1669    1783    1793    1922    1954    2048    2079    2438 
   Size:         89     114      10     129      32      94      31     359

Cuts at:   2438    2450    2690    2912    3117 
   Size:         12     240     222     205

  Fragments arranged by size: 

                 359     359     324     255     240     226     222     216
                 205     137     129     114      94      89      36      32
                  31      27      12      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2057    2534    2774    3117 
   Size:       2057     477     240     343

  Fragments arranged by size: 

                2057     477     343     240
 
BsrBI CCG'CTC

Cuts at:      0     503     889    1959    2916    3117 
   Size:        503     386    1070     957     201

  Fragments arranged by size: 

                1070     957     503     386     201
 
BsrDI GCAATG_nn'

Cuts at:      0    1211    2091    3117 
   Size:       1211     880    1026

  Fragments arranged by size: 

                1211    1026     880
 
BsrFI r'CCGG_y

Cuts at:      0    1625    1646    2313    3080    3117 
   Size:       1625      21     667     767      37

  Fragments arranged by size: 

                1625     767     667      37      21
 
BsrGI T'GTAC_A

Cuts at:      0    1433    3117 
   Size:       1433    1684
 
BssHII G'CGCG_C

Cuts at:      0    2779    3117 
   Size:       2779     338
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     930    1008    1222    3117 
   Size:        930      78     214    1895

  Fragments arranged by size: 

                1895     930     214      78
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     201    1425    2458    2993    3117 
   Size:        201    1224    1033     535     124

  Fragments arranged by size: 

                1224    1033     535     201     124
 
BstYI r'GATC_y

Cuts at:      0     831    1698    2672    2712    3117 
   Size:        831     867     974      40     405

  Fragments arranged by size: 

                 974     867     831     405      40
 
BtgI C'CryG_G

Cuts at:      0     180     211     611     812    1736    1817    2795    3117 
   Size:        180      31     400     201     924      81     978     322

  Fragments arranged by size: 

                 978     924     400     322     201     180      81      31
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1155    2212    3117 
   Size:       1155    1057     905

  Fragments arranged by size: 

                1155    1057     905
 
BtsI GCAGTG_nn'

Cuts at:      0      19    1223    1934    2764    3117 
   Size:         19    1204     711     830     353

  Fragments arranged by size: 

                1204     830     711     353      19
 
ClaI AT'CG_AT

Cuts at:      0    1373    3117 
   Size:       1373    1744
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2314    3117 
   Size:       2314     803
 
EaeI y'GGCC_r

Cuts at:      0       2     191     279     504     809    1058    1245    2039 
   Size:          2     189      88     225     305     249     187     794

Cuts at:   2039    3047    3082    3117 
   Size:       1008      35      35

  Fragments arranged by size: 

                1008     794     305     249     225     189     187      88
                  35      35       2
 
EagI C'GGCC_G

Cuts at:      0     504    3117 
   Size:        504    2613
 
EarI CTCTTCn'nnn_

Cuts at:      0    1498    2219    3101    3117 
   Size:       1498     721     882      16

  Fragments arranged by size: 

                1498     882     721      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1721    3117 
   Size:       1721    1396
 
EcoICRI GAG'CTC

Cuts at:      0    1441    2448    3117 
   Size:       1441    1007     669

  Fragments arranged by size: 

                1441    1007     669
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61      65     285     411     588     671     750    1912 
   Size:         61       4     220     126     177      83      79    1162

Cuts at:   1912    2005    2548    2692    3117 
   Size:         93     543     144     425

  Fragments arranged by size: 

                1162     543     425     220     177     144     126      93
                  83      79      61       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     679    2053    3117 
   Size:        679    1374    1064

  Fragments arranged by size: 

                1374    1064     679
 
EcoO109I rG'GnC_Cy

Cuts at:      0      64     130     223    1099    1237    1308    2021    2076 
   Size:         64      66      93     876     138      71     713      55

Cuts at:   2076    2422    2559    3117 
   Size:        346     137     558

  Fragments arranged by size: 

                 876     713     558     346     138     137      93      71
                  66      64      55
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3081    3117 
   Size:       3081      36
 
HaeII r_GCGC'y

Cuts at:      0     209    1022    2063    2743    3117 
   Size:        209     813    1041     680     374

  Fragments arranged by size: 

                1041     813     680     374     209
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     179     211     688     720     909     941     990    1022 
   Size:        179      32     477      32     189      32      49      32

Cuts at:   1022    1281    1313    2227    2259    3117 
   Size:        259      32     914      32     858

  Fragments arranged by size: 

                 914     858     477     259     189     179      49      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     987    2589    3117 
   Size:        987    1602     528

  Fragments arranged by size: 

                1602     987     528
 
KasI G'GCGC_C

Cuts at:      0     205    2059    2739    3117 
   Size:        205    1854     680     378

  Fragments arranged by size: 

                1854     680     378     205
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1545    2546    3117 
   Size:       1545    1001     571

  Fragments arranged by size: 

                1545    1001     571
 
MscI TGG'CCA

Cuts at:      0       4     193    3049    3117 
   Size:          4     189    2856      68

  Fragments arranged by size: 

                2856     189      68       4
 
MslI CAynn'nnrTG

Cuts at:      0     199     556    1216    1555    1735    1864    2441    2456 
   Size:        199     357     660     339     180     129     577      15

Cuts at:   2456    3117 
   Size:        661

  Fragments arranged by size: 

                 661     660     577     357     339     199     180     129
                  15
 
MspA1I CmG'CkG

Cuts at:      0     213     389    1620    2253    2417    2790    2955    3117 
   Size:        213     176    1231     633     164     373     165     162

  Fragments arranged by size: 

                1231     633     373     213     176     165     164     162
            
 
NaeI GCC'GGC

Cuts at:      0    1648    3117 
   Size:       1648    1469
 
NarI GG'CG_CC

Cuts at:      0     206    2060    2740    3117 
   Size:        206    1854     680     377

  Fragments arranged by size: 

                1854     680     377     206
 
NcoI C'CATG_G

Cuts at:      0     180    1736    2795    3117 
   Size:        180    1556    1059     322

  Fragments arranged by size: 

                1556    1059     322     180
 
NgoMIV G'CCGG_C

Cuts at:      0    1646    3117 
   Size:       1646    1471
 
NotI GC'GGCC_GC

Cuts at:      0     504    3117 
   Size:        504    2613
 
NruI TCG'CGA

Cuts at:      0    2197    3117 
   Size:       2197     920
 
NspI r_CATG'y

Cuts at:      0    2073    3117 
   Size:       2073    1044
 
PasI CC'CwG_GG

Cuts at:      0     219     882    1179    2055    2464    2553    3117 
   Size:        219     663     297     876     409      89     564

  Fragments arranged by size: 

                 876     663     564     409     297     219      89
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     369    1110    1260    1311    1473    3117 
   Size:        369     741     150      51     162    1644

  Fragments arranged by size: 

                1644     741     369     162     150      51
 
PfoI T'CCnGG_A

Cuts at:      0    1310    1766    1889    1982    2502    2537    3117 
   Size:       1310     456     123      93     520      35     580

  Fragments arranged by size: 

                1310     580     520     456     123      93      35
 
PmlI CAC'GTG

Cuts at:      0     738    3117 
   Size:        738    2379
 
PpuMI rG'GwC_Cy

Cuts at:      0      64    1237    1308    2422    3117 
   Size:         64    1173      71    1114     695

  Fragments arranged by size: 

                1173    1114     695      71      64
 
PspOMI G'GGCC_C

Cuts at:      0     222    2075    3117 
   Size:        222    1853    1042

  Fragments arranged by size: 

                1853    1042     222
 
PspXI vC'TCGA_Gb

Cuts at:      0    2700    3117 
   Size:       2700     417
 
PstI C_TGCA'G

Cuts at:      0    1091    1619    1994    2258    2771    2867    3117 
   Size:       1091     528     375     264     513      96     250

  Fragments arranged by size: 

                1091     528     513     375     264     250      96
 
PvuII CAG'CTG

Cuts at:      0     389    1620    2253    2417    2955    3117 
   Size:        389    1231     633     164     538     162

  Fragments arranged by size: 

                1231     633     538     389     164     162
 
RsrII CG'GwC_CG

Cuts at:      0    2316    3117 
   Size:       2316     801
 
SacI G_AGCT'C

Cuts at:      0    1443    2450    3117 
   Size:       1443    1007     667

  Fragments arranged by size: 

                1443    1007     667
 
SacII CC_GC'GG

Cuts at:      0     214    3117 
   Size:        214    2903
 
SanDI GG'GwC_CC

Cuts at:      0    1237    2422    3117 
   Size:       1237    1185     695

  Fragments arranged by size: 

                1237    1185     695
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1498    3117 
   Size:       1498    1619
 
SbfI CC_TGCA'GG

Cuts at:      0    2771    3117 
   Size:       2771     346
 
ScaI AGT'ACT

Cuts at:      0     520     781    3117 
   Size:        520     261    2336

  Fragments arranged by size: 

                2336     520     261
 
SexAI A'CCwGG_T

Cuts at:      0    1421    3117 
   Size:       1421    1696
 
SfcI C'TryA_G

Cuts at:      0     842    1087    1615    1990    2254    2767    2863    3117 
   Size:        842     245     528     375     264     513      96     254

  Fragments arranged by size: 

                 842     528     513     375     264     254     245      96
            
 
SfoI GGC'GCC

Cuts at:      0     207    2061    2741    3117 
   Size:        207    1854     680     376

  Fragments arranged by size: 

                1854     680     376     207
 
SmlI C'TyrA_G

Cuts at:      0     705    2596    2700    3117 
   Size:        705    1891     104     417

  Fragments arranged by size: 

                1891     705     417     104
 
StuI AGG'CCT

Cuts at:      0    1459    2844    3117 
   Size:       1459    1385     273

  Fragments arranged by size: 

                1459    1385     273
 
StyI C'CwwG_G

Cuts at:      0     180     363    1736    2795    2966    3117 
   Size:        180     183    1373    1059     171     151

  Fragments arranged by size: 

                1373    1059     183     180     171     151
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      36     369    1064    1102    1876    2985    2995    3004 
   Size:         36     333     695      38     774    1109      10       9

Cuts at:   3004    3117 
   Size:        113

  Fragments arranged by size: 

                1109     774     695     333     113      38      36      10
                   9
 
TatI w'GTAC_w

Cuts at:      0     518     779    1433    2746    3117 
   Size:        518     261     654    1313     371

  Fragments arranged by size: 

                1313     654     518     371     261
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     149     181     620     652     727     759    1871    1903 
   Size:        149      32     439      32      75      32    1112      32

Cuts at:   1903    2414    2446    2942    2974    3117 
   Size:        511      32     496      32     143

  Fragments arranged by size: 

                1112     511     496     439     149     143      75      32
                  32      32      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1176    1561    1692    3042    3117 
   Size:       1176     385     131    1350      75

  Fragments arranged by size: 

                1350    1176     385     131      75
 
XhoI C'TCGA_G

Cuts at:      0     705    2700    3117 
   Size:        705    1995     417

  Fragments arranged by size: 

                1995     705     417
 
ZraI GAC'GTC

Cuts at:      0     399     984    3117 
   Size:        399     585    2133

  Fragments arranged by size: 

                2133     585     399
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI
    PvuII    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI     BcgI     BclI    BfrBI     BmtI
    BsaWI    BsiWI    BspEI    BspHI    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    CspCI     DraI   DraIII    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI     PsrI
     PvuI     SalI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XmaI     XmnI
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