Restriction map for
pFN21AB5979
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BbeI BbvCI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoRI EcoRV FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 20 CGCG CG^CG
Alw44I 670 GTGCAC G^TGCAC
Aor51HI 148 AGCGCT AGC^GCT
ApaI 989 GGGCCC GGGCC^C
ApaLI 670 GTGCAC G^TGCAC
Asp700I 306 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 419 TGGCCA TGG^CCA
BamHI 396 GGATCC G^GATCC
BanI 432 GGTGCC G^G[CT][GA]CC
BbrPI 673 CACGTG CAC^GTG
BbsI 1279 GAAGAC GAAGAC(2/6)
BciVI 1536 GTATCC GTATCC(6/5)
BcnI 537 CCCGG CC^[GC]GG
BpuAI 1279 GAAGAC GAAGAC(2/6)
BsaAI 673 CACGTG [CT]AC^GT[GA]
BsaBI 1291 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1067 GAATGC GAATGC(1/-1)
BspDI 424 ATCGAT AT^CGAT
BspHI 1217 TCATGA T^CATGA
BstAPI 642 GCAATCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1251 GGTCACC G^GT[ACGT]ACC
BstPI 1251 GGTCACC G^GT[ACGT]ACC
BstUI 20 CGCG CG^CG
BstXI 422 CCATCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 424 ATCGAT AT^CGAT
DraIII 944 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 419 TGGCCA [CT]^GGCC[GA]
Eco47III 148 AGCGCT AGC^GCT
EcoO65I 1251 GGTCACC G^GT[ACGT]ACC
EcoT22I 649 ATGCAT ATGCA^T
HaeII 148 AGCGCT [GA]GCGC^[CT]
MamI 1291 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 419 TGGCCA TGG^CCA
MscI 419 TGGCCA TGG^CCA
MslI 423 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 231 CAGCGG C[AC]G^C[GT]G
MvnI 20 CGCG CG^CG
NciI 537 CCCGG CC^[GC]GG
NcoI 895 CCATGG C^CATGG
NsiI 649 ATGCAT ATGCA^T
PmaCI 673 CACGTG CAC^GTG
PmlI 673 CACGTG CAC^GTG
PshAI 1486 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 152 CTGCAG CTGCA^G
RcaI 1217 TCATGA T^CATGA
SacI 9 GAGCTC GAGCT^C
SapI 159 GAAGAGC GCTCTTC(1/4)
SspI 1354 AATATT AAT^ATT
StuI 901 AGGCCT AGG^CCT
XbaI 1447 TCTAGA T^CTAGA
XmnI 306 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 9 GAGCTC G[AT]GC[AT]^C
670 GTGCAC
BclI 335 TGATCA T^GATCA
1290 TGATCA
BpmI 968 CTCCAG CTGGAG(16/14)
1284 CTCCAG
BseRI 130 GAGGAG GAGGAG(10/8)
133 GAGGAG
BsiHKAI 9 GAGCTC G[AT]GC[AT]^C
670 GTGCAC
BsmFI 325 GGGAC GGGAC(10/14)
705 GTCCC
BspMI 1088 ACCTGC ACCTGC(4/8)
1103 ACCTGC
BsrFI 435 GCCGGT [GA]^CCGG[CT]
1164 ACCGGC
BstYI 292 GGATCT [GA]^GATC[CT]
396 GGATCC
Cfr10I 435 GCCGGT [GA]^CCGG[CT]
1164 ACCGGC
Cfr13I 989 GGGCC G^G[ACGT]CC
990 GGCCC
DraI 751 TTTAAA TTT^AAA
922 TTTAAA
DraII 988 GGGGCCC [GA]G^G[ACGT]CC[CT]
989 GGGCCCC
DsaI 32 CCGTGG C^C[GA][CT]GG
895 CCATGG
EcoO109I 988 GGGGCCC [GA]G^G[ACGT]CC[CT]
989 GGGCCCC
FauI 105 GCGGG CCCGC(4/6)
349 GCGGG
FbaI 335 TGATCA T^GATCA
1290 TGATCA
HincII 697 GTTGAC GT[CT]^[GA]AC
1099 GTCAAC
HindII 697 GTTGAC GT[CT]^[GA]AC
1099 GTCAAC
MaeII 461 ACGT A^CGT
674 ACGT
MflI 292 GGATCT [GA]^GATC[CT]
396 GGATCC
PleI 541 GACTC GAGTC(4/5)
1282 GACTC
Sau96I 989 GGGCC G^G[ACGT]CC
990 GGCCC
SfaNI 567 GATGC GCATC(5/9)
512 GCATC
SfcI 152 CTGCAG C^T[GA][CT]AG
453 CTATAG
TaqI 425 TCGA T^CGA
917 TCGA
TthHB8I 425 TCGA T^CGA
917 TCGA
XcmI 605 CCAACAATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1129 CCAACTAATTTTTGG
XhoII 292 GGATCT [GA]^GATC[CT]
396 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BanII BbvI BfaI BmyI BsaI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI