(Circular) (Six-base) MAPSORT of: pFN21AB5979.seq Check: 3315 from: 1 to: 6265 pFN21AB5979 6265 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2754 3303 4328 5712 5980 1632 Size: 1122 549 1025 1384 268 1917 Fragments arranged by size: 1917 1384 1122 1025 549 268 AfeI AGC'GCT Cuts at: 2155 2155 Size: 6265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3415 1184 Size: 2231 4034 AlwNI CAG_nnn'CTG Cuts at: 4008 5580 4008 Size: 1572 4693 ApaI G_GGCC'C Cuts at: 2998 2998 Size: 6265 ApaLI G'TGCA_C Cuts at: 2675 4322 5478 2675 Size: 1647 1156 3462 Fragments arranged by size: 3462 1647 1156 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2557 2670 3590 1870 Size: 73 76 538 113 920 4545 Fragments arranged by size: 4545 920 538 113 76 73 BamHI G'GATC_C Cuts at: 2401 3595 3962 2401 Size: 1194 367 4704 Fragments arranged by size: 4704 1194 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2437 3586 5041 618 Size: 325 217 627 650 1149 1455 1842 Fragments arranged by size: 1842 1455 1149 650 627 325 217 BanII G_rGCy'C Cuts at: 1391 2018 2202 2998 3584 1391 Size: 627 184 796 586 4072 Fragments arranged by size: 4072 796 627 586 184 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3291 928 Size: 971 1392 3902 Fragments arranged by size: 3902 1392 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3552 4157 5367 3552 Size: 605 1210 4450 Fragments arranged by size: 4450 1210 605 BclI T'GATC_A Cuts at: 1548 2340 3295 1548 Size: 792 955 4518 Fragments arranged by size: 4518 955 792 BfrBI ATG'CAT Cuts at: 2656 2656 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2679 2998 4326 5482 1790 Size: 889 319 1328 1156 2573 Fragments arranged by size: 2573 1328 1156 889 319 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2958 3274 4925 1239 Size: 1719 316 1651 2579 Fragments arranged by size: 2579 1719 1651 316 Bpu10I CC'TnA_GC Cuts at: 2103 2103 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4390 5255 5553 4390 Size: 865 298 5102 Fragments arranged by size: 5102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2050 2558 2885 4928 882 Size: 617 551 508 327 2043 2219 Fragments arranged by size: 2219 2043 617 551 508 327 BsaAI yAC'GTr Cuts at: 493 1204 2680 4056 493 Size: 711 1476 1376 2702 Fragments arranged by size: 2702 1476 1376 711 BsaBI GATnn'nnATC Cuts at: 3300 6259 3300 Size: 2959 3306 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6040 Fragments arranged by size: 6040 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2153 1186 Size: 964 3 5298 Fragments arranged by size: 5298 964 3 BseYI C'CCAG_C Cuts at: 2060 2495 5468 5964 2060 Size: 435 2973 496 2361 Fragments arranged by size: 2973 2361 496 435 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5692 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2018 2679 4326 4411 5482 2018 Size: 661 1647 85 1071 2801 Fragments arranged by size: 2801 1647 1071 661 85 BsmI GAATG_Cn' Cuts at: 3078 3776 3869 3078 Size: 698 93 5474 Fragments arranged by size: 5474 698 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2202 2679 2998 3584 4326 4411 Size: 399 228 184 477 319 586 742 85 Cuts at: 4411 5482 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 586 477 399 319 228 184 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 3222 4154 5884 3222 Size: 932 1730 3603 Fragments arranged by size: 3603 1730 932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3102 3117 3620 844 Size: 2258 15 503 3489 Fragments arranged by size: 3489 2258 503 15 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2440 3169 4909 5096 1700 Size: 110 630 729 1740 187 2869 Fragments arranged by size: 2869 1740 729 630 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 2653 794 Size: 1859 4406 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 3256 1184 Size: 2072 4193 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2434 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2297 2401 3595 3962 4347 4364 5816 5902 Size: 325 104 1194 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 325 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2900 513 Size: 552 939 33 863 3878 Fragments arranged by size: 3878 939 863 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 2704 2968 3781 4575 4595 2704 Size: 264 813 794 20 4374 Fragments arranged by size: 4374 813 794 264 20 ClaI AT'CG_AT Cuts at: 1554 2430 1554 Size: 876 5389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 2758 2929 3569 2758 Size: 171 640 5454 Fragments arranged by size: 5454 640 171 DraIII CAC_nnn'GTG Cuts at: 2954 2954 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 3712 4601 Size: 54 1425 50 275 18 594 1288 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1288 889 594 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2159 2262 4195 1353 Size: 774 32 103 1933 3423 Fragments arranged by size: 3423 1933 774 103 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2108 2139 2377 4852 5226 5372 Size: 1064 138 519 31 238 2475 374 146 Cuts at: 5372 387 Size: 1280 Fragments arranged by size: 2475 1280 1064 519 374 238 146 138 31 EcoICRI GAG'CTC Cuts at: 2016 2016 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2754 2958 3274 3303 4328 4925 5712 Size: 393 1122 204 316 29 1025 597 787 Cuts at: 5712 5980 1239 Size: 268 1524 Fragments arranged by size: 1524 1122 1025 787 597 393 316 268 204 29 EcoO109I rG'GnC_Cy Cuts at: 2994 2995 2994 Size: 1 6264 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2847 2847 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2157 5412 1678 Size: 479 3255 2531 Fragments arranged by size: 3255 2531 479 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373 Size: 32 135 32 3334 32 42 32 2626 Fragments arranged by size: 3334 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2704 3106 3609 3855 669 Size: 988 281 766 402 503 246 3079 Fragments arranged by size: 3079 988 766 503 402 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 3158 3495 5379 5563 1305 Size: 1074 779 337 1884 184 2007 Fragments arranged by size: 2007 1884 1074 779 337 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 10 Size: 54 1425 343 594 3849 Fragments arranged by size: 3849 1425 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 4223 4582 4741 518 Size: 648 1266 1791 359 159 2042 Fragments arranged by size: 2042 1791 1266 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2238 3681 4358 5506 5751 1606 Size: 632 1443 677 1148 245 2120 Fragments arranged by size: 2120 1443 1148 677 632 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2900 513 Size: 552 939 896 3878 Fragments arranged by size: 3878 939 896 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NsiI A_TGCA'T Cuts at: 2658 2658 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3623 5168 1132 Size: 97 2394 1545 2229 Fragments arranged by size: 2394 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3250 1348 Size: 1902 4363 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PmlI CAC'GTG Cuts at: 2680 2680 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3495 1613 Size: 1882 4383 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2994 2994 Size: 6265 PstI C_TGCA'G Cuts at: 830 2161 3617 830 Size: 1331 1456 3478 Fragments arranged by size: 3478 1456 1331 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 SacI G_AGCT'C Cuts at: 2018 2018 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 2159 2159 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 2157 2458 3613 4748 5429 5620 826 Size: 219 1112 301 1155 1135 681 191 1471 Fragments arranged by size: 1471 1155 1135 1112 681 301 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4369 5270 5532 820 Size: 197 926 2426 901 262 1553 Fragments arranged by size: 2426 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 3361 4189 5 Size: 47 3309 828 2081 Fragments arranged by size: 3309 2081 828 47 StuI AGG'CCT Cuts at: 1517 2908 1517 Size: 1391 4874 StyI C'CwwG_G Cuts at: 513 1065 2004 2046 2900 3067 3704 513 Size: 552 939 42 854 167 637 3074 Fragments arranged by size: 3074 939 854 637 552 167 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4292 4309 4462 4647 5092 325 Size: 1255 2712 17 153 185 445 1498 Fragments arranged by size: 2712 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2661 4511 6223 Size: 274 80 33 51 1443 684 1850 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 1850 1712 1443 684 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3452 3601 3452 Size: 149 6116 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2617 3141 2617 Size: 524 5741 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 2315 4394 2315 Size: 2079 4186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI