Restriction map for
pFN21AB5856
(Linear) (Six-base) MAPSORT of: KIBB5856.seq Check: 6186 from: 1 to: 1467
KIBB5856.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1094 1467
Size: 428 666 373
Fragments arranged by size:
666 428 373
AflIII A'CryG_T
Cuts at: 0 663 1380 1467
Size: 663 717 87
Fragments arranged by size:
717 663 87
ApoI r'AATT_y
Cuts at: 0 207 285 352 627 1285 1459 1467
Size: 207 78 67 275 658 174 8
Fragments arranged by size:
658 275 207 174 78 67 8
AseI AT'TA_AT
Cuts at: 0 818 1467
Size: 818 649
AvaI C'yCGr_G
Cuts at: 0 1098 1467
Size: 1098 369
BanI G'GyrC_C
Cuts at: 0 58 1467
Size: 58 1409
BanII G_rGCy'C
Cuts at: 0 1334 1467
Size: 1334 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 1016 1467
Size: 1016 451
BciVI GTATCCnnnnn_n'
Cuts at: 0 627 1467
Size: 627 840
BclI T'GATC_A
Cuts at: 0 264 1215 1467
Size: 264 951 252
Fragments arranged by size:
951 264 252
BfrBI ATG'CAT
Cuts at: 0 1002 1467
Size: 1002 465
BlpI GC'TnA_GC
Cuts at: 0 781 1467
Size: 781 686
Bme1580I G_kGCm'C
Cuts at: 0 61 1467
Size: 61 1406
BmgBI CAC'GTC
Cuts at: 0 926 1092 1467
Size: 926 166 375
Fragments arranged by size:
926 375 166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1179 1206 1467
Size: 965 214 27 261
Fragments arranged by size:
965 261 214 27
BsaBI GATnn'nnATC
Cuts at: 0 482 1467
Size: 482 985
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 1467
Size: 424 1043
BsiHKAI G_wGCw'C
Cuts at: 0 1334 1467
Size: 1334 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 455 1467
Size: 455 1012
Bsp1286I G_dGCh'C
Cuts at: 0 61 1334 1467
Size: 61 1273 133
Fragments arranged by size:
1273 133 61
BspHI T'CATG_A
Cuts at: 0 422 1467
Size: 422 1045
BsrGI T'GTAC_A
Cuts at: 0 512 1467
Size: 512 955
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1254 1467
Size: 1254 213
BstYI r'GATC_y
Cuts at: 0 66 545 1392 1467
Size: 66 479 847 75
Fragments arranged by size:
847 479 75 66
BtgI C'CryG_G
Cuts at: 0 62 1467
Size: 62 1405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1158 1467
Size: 1158 309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1344 1379 1467
Size: 1344 35 88
Fragments arranged by size:
1344 88 35
DraI TTT'AAA
Cuts at: 0 1181 1467
Size: 1181 286
EarI CTCTTCn'nnn_
Cuts at: 0 962 1467
Size: 962 505
EcoICRI GAG'CTC
Cuts at: 0 1332 1467
Size: 1332 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 965 1094 1179 1206 1467
Size: 428 537 129 85 27 261
Fragments arranged by size:
537 428 261 129 85 27
EcoO109I rG'GnC_Cy
Cuts at: 0 165 1467
Size: 165 1302
EcoRI G'AATT_C
Cuts at: 0 207 627 1467
Size: 207 420 840
Fragments arranged by size:
840 420 207
EcoRV GAT'ATC
Cuts at: 0 399 1467
Size: 399 1068
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 754 899 1467
Size: 754 145 568
Fragments arranged by size:
754 568 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 961 993 1316 1348 1467
Size: 961 32 323 32 119
Fragments arranged by size:
961 323 119 32 32
MfeI C'AATT_G
Cuts at: 0 107 1467
Size: 107 1360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 1467
Size: 297 1170
MslI CAynn'nnrTG
Cuts at: 0 272 508 1467
Size: 272 236 959
Fragments arranged by size:
959 272 236
MspA1I CmG'CkG
Cuts at: 0 64 1250 1467
Size: 64 1186 217
Fragments arranged by size:
1186 217 64
NsiI A_TGCA'T
Cuts at: 0 1004 1467
Size: 1004 463
NspI r_CATG'y
Cuts at: 0 667 1002 1384 1467
Size: 667 335 382 83
Fragments arranged by size:
667 382 335 83
PciI A'CATG_T
Cuts at: 0 663 1380 1467
Size: 663 717 87
Fragments arranged by size:
717 663 87
PfoI T'CCnGG_A
Cuts at: 0 1017 1467
Size: 1017 450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1116 1148 1467
Size: 1116 32 319
Fragments arranged by size:
1116 319 32
PpuMI rG'GwC_Cy
Cuts at: 0 165 1467
Size: 165 1302
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 717 749 1467
Size: 717 32 718
Fragments arranged by size:
718 717 32
PvuII CAG'CTG
Cuts at: 0 1250 1467
Size: 1250 217
SacI G_AGCT'C
Cuts at: 0 1334 1467
Size: 1334 133
SacII CC_GC'GG
Cuts at: 0 65 1467
Size: 65 1402
SapI GCTCTTCn'nnn_
Cuts at: 0 962 1467
Size: 962 505
ScaI AGT'ACT
Cuts at: 0 637 1467
Size: 637 830
SmaI CCC'GGG
Cuts at: 0 1100 1467
Size: 1100 367
SphI G_CATG'C
Cuts at: 0 1002 1467
Size: 1002 465
SspI AAT'ATT
Cuts at: 0 237 258 845 1083 1467
Size: 237 21 587 238 384
Fragments arranged by size:
587 384 238 237 21
TatI w'GTAC_w
Cuts at: 0 512 635 1467
Size: 512 123 832
Fragments arranged by size:
832 512 123
Tth111I GACn'n_nGTC
Cuts at: 0 1093 1467
Size: 1093 374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1162 1467
Size: 1162 305
XmaI C'CCGG_G
Cuts at: 0 1098 1467
Size: 1098 369
XmnI GAAnn'nnTTC
Cuts at: 0 1082 1203 1467
Size: 1082 121 264
Fragments arranged by size:
1082 264 121
Enzymes that do cut:
AcuI AflIII ApoI AseI AvaI BanI BanII BbsI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BpmI BsaBI
BseRI BsiHKAI BsmBI Bsp1286I BspHI BsrGI BstXI BstYI
BtgI BtgZI CspCI DraI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I MfeI MmeI MslI MspA1I
NsiI NspI PciI PfoI PpiI PpuMI PsrI PvuII
SacI SacII SapI ScaI SmaI SphI SspI TatI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BglI
BglII BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraIII DrdI
EaeI EagI EciI EcoNI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI
PstI PvuI RsrII SalI SanDI SbfI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI
StuI StyI SwaI TaqII TstI XbaI XhoI ZraI