Restriction map for
pFN21AE0988
(Linear) (Six-base) MAPSORT of: KIEE0988.seq Check: 9347 from: 1 to: 1857
KIEE0988.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 29 432 1857
Size: 29 403 1425
Fragments arranged by size:
1425 403 29
Acc65I G'GTAC_C
Cuts at: 0 488 1857
Size: 488 1369
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 736 742 769 1015 1132 1857
Size: 91 645 6 27 246 117 725
Fragments arranged by size:
725 645 246 117 91 27 6
AflIII A'CryG_T
Cuts at: 0 505 1857
Size: 505 1352
AleI CACnn'nnGTG
Cuts at: 0 937 1686 1857
Size: 937 749 171
Fragments arranged by size:
937 749 171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 264 577 1417 1542 1857
Size: 264 313 840 125 315
Fragments arranged by size:
840 315 313 264 125
AlwNI CAG_nnn'CTG
Cuts at: 0 117 793 1857
Size: 117 676 1064
Fragments arranged by size:
1064 676 117
ApaI G_GGCC'C
Cuts at: 0 1820 1857
Size: 1820 37
ApaLI G'TGCA_C
Cuts at: 0 151 1372 1857
Size: 151 1221 485
Fragments arranged by size:
1221 485 151
AvaI C'yCGr_G
Cuts at: 0 322 670 1857
Size: 322 348 1187
Fragments arranged by size:
1187 348 322
BanI G'GyrC_C
Cuts at: 0 199 488 676 1459 1857
Size: 199 289 188 783 398
Fragments arranged by size:
783 398 289 199 188
BanII G_rGCy'C
Cuts at: 0 98 248 737 952 974 1820 1857
Size: 98 150 489 215 22 846 37
Fragments arranged by size:
846 489 215 150 98 37 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 333 622 1857
Size: 333 289 1235
Fragments arranged by size:
1235 333 289
BbvCI CC'TCA_GC
Cuts at: 0 565 1841 1857
Size: 565 1276 16
Fragments arranged by size:
1276 565 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 865 1857
Size: 865 992
BfrBI ATG'CAT
Cuts at: 0 1529 1857
Size: 1529 328
BglI GCCn_nnn'nGGC
Cuts at: 0 790 1857
Size: 790 1067
BlpI GC'TnA_GC
Cuts at: 0 1488 1857
Size: 1488 369
Bme1580I G_kGCm'C
Cuts at: 0 155 589 679 1123 1376 1681 1820 1857
Size: 155 434 90 444 253 305 139 37
Fragments arranged by size:
444 434 305 253 155 139 90 37
BmgBI CAC'GTC
Cuts at: 0 389 1095 1857
Size: 389 706 762
Fragments arranged by size:
762 706 389
BmrI ACTGGGnnnn_n'
Cuts at: 0 1465 1857
Size: 1465 392
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 276 1857
Size: 276 1581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 433 1375 1533 1587 1857
Size: 418 15 942 158 54 270
Fragments arranged by size:
942 418 270 158 54 15
Bpu10I CC'TnA_GC
Cuts at: 0 565 881 1841 1857
Size: 565 316 960 16
Fragments arranged by size:
960 565 316 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1492 1857
Size: 958 534 365
Fragments arranged by size:
958 534 365
BsaAI yAC'GTr
Cuts at: 0 378 1857
Size: 378 1479
BsaHI Gr'CG_yC
Cuts at: 0 359 1208 1857
Size: 359 849 649
Fragments arranged by size:
849 649 359
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1582 1612 1857
Size: 1582 30 245
Fragments arranged by size:
1582 245 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 526 982 1000 1306 1372 1857
Size: 76 450 456 18 306 66 485
Fragments arranged by size:
485 456 450 306 76 66 18
BseYI C'CCAG_C
Cuts at: 0 126 810 1249 1857
Size: 126 684 439 608
Fragments arranged by size:
684 608 439 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 1309 1857
Size: 1249 60 548
Fragments arranged by size:
1249 548 60
BsiEI CG_ry'CG
Cuts at: 0 313 919 1857
Size: 313 606 938
Fragments arranged by size:
938 606 313
BsiHKAI G_wGCw'C
Cuts at: 0 68 155 248 974 1376 1857
Size: 68 87 93 726 402 481
Fragments arranged by size:
726 481 402 93 87 68
BsmI GAATG_Cn'
Cuts at: 0 665 1531 1857
Size: 665 866 326
Fragments arranged by size:
866 665 326
BsmBI CGTCTCn'nnnn_
Cuts at: 0 350 837 1857
Size: 350 487 1020
Fragments arranged by size:
1020 487 350
Bsp1286I G_dGCh'C
Cuts at: 0 68 98 155 248 589 679 737 952
Size: 68 30 57 93 341 90 58 215
Cuts at: 952 974 1123 1376 1681 1820 1857
Size: 22 149 253 305 139 37
Fragments arranged by size:
341 305 253 215 149 139 93 90
68 58 57 37 30 22
BspHI T'CATG_A
Cuts at: 0 1712 1857
Size: 1712 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 29 299 432 1857
Size: 29 270 133 1425
Fragments arranged by size:
1425 270 133 29
BsrBI CCG'CTC
Cuts at: 0 477 1487 1506 1857
Size: 477 1010 19 351
Fragments arranged by size:
1010 477 351 19
BsrDI GCAATG_nn'
Cuts at: 0 209 455 1314 1857
Size: 209 246 859 543
Fragments arranged by size:
859 543 246 209
BsrFI r'CCGG_y
Cuts at: 0 214 237 308 1412 1857
Size: 214 23 71 1104 445
Fragments arranged by size:
1104 445 214 71 23
BssSI C'ACGA_G
Cuts at: 0 148 472 933 1857
Size: 148 324 461 924
Fragments arranged by size:
924 461 324 148
BstAPI GCAn_nnn'nTGC
Cuts at: 0 159 302 662 1167 1459 1776 1857
Size: 159 143 360 505 292 317 81
Fragments arranged by size:
505 360 317 292 159 143 81
BstEII G'GTnAC_C
Cuts at: 0 1578 1857
Size: 1578 279
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1455 1809 1857
Size: 1455 354 48
Fragments arranged by size:
1455 354 48
BstYI r'GATC_y
Cuts at: 0 1295 1727 1857
Size: 1295 432 130
Fragments arranged by size:
1295 432 130
Bsu36I CC'TnA_GG
Cuts at: 0 786 1857
Size: 786 1071
BtgI C'CryG_G
Cuts at: 0 11 362 571 913 926 1337 1763 1857
Size: 11 351 209 342 13 411 426 94
Fragments arranged by size:
426 411 351 342 209 94 13 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1574 1857
Size: 1574 283
BtsI GCAGTG_nn'
Cuts at: 0 777 1478 1857
Size: 777 701 379
Fragments arranged by size:
777 701 379
DraIII CAC_nnn'GTG
Cuts at: 0 159 1332 1459 1586 1857
Size: 159 1173 127 127 271
Fragments arranged by size:
1173 271 159 127 127
EaeI y'GGCC_r
Cuts at: 0 310 916 1097 1313 1501 1605 1799 1857
Size: 310 606 181 216 188 104 194 58
Fragments arranged by size:
606 310 216 194 188 181 104 58
EagI C'GGCC_G
Cuts at: 0 310 916 1857
Size: 310 606 941
Fragments arranged by size:
941 606 310
EarI CTCTTCn'nnn_
Cuts at: 0 103 994 1136 1857
Size: 103 891 142 721
Fragments arranged by size:
891 721 142 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 103 742 1857
Size: 103 639 1115
Fragments arranged by size:
1115 639 103
EcoICRI GAG'CTC
Cuts at: 0 246 972 1857
Size: 246 726 885
Fragments arranged by size:
885 726 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 418 433 736 742 769 1015 1132
Size: 91 327 15 303 6 27 246 117
Cuts at: 1132 1375 1533 1587 1857
Size: 243 158 54 270
Fragments arranged by size:
327 303 270 246 243 158 117 91
54 27 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 415 751 1857
Size: 415 336 1106
Fragments arranged by size:
1106 415 336
EcoO109I rG'GnC_Cy
Cuts at: 0 408 435 1224 1816 1817 1857
Size: 408 27 789 592 1 40
Fragments arranged by size:
789 592 408 40 27 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1005 1857
Size: 1005 852
FspI TGC'GCA
Cuts at: 0 295 1078 1857
Size: 295 783 779
Fragments arranged by size:
783 779 295
HaeII r_GCGC'y
Cuts at: 0 189 1857
Size: 189 1668
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 822 854 1582 1614 1857
Size: 822 32 728 32 243
Fragments arranged by size:
822 728 243 32 32
HincII GTy'rAC
Cuts at: 0 830 1410 1857
Size: 830 580 447
Fragments arranged by size:
830 580 447
HpaI GTT'AAC
Cuts at: 0 1410 1857
Size: 1410 447
KpnI G_GTAC'C
Cuts at: 0 492 1857
Size: 492 1365
MluI A'CGCG_T
Cuts at: 0 505 1857
Size: 505 1352
MscI TGG'CCA
Cuts at: 0 1099 1801 1857
Size: 1099 702 56
Fragments arranged by size:
1099 702 56
MslI CAynn'nnrTG
Cuts at: 0 937 1105 1243 1342 1524 1686 1857
Size: 937 168 138 99 182 162 171
Fragments arranged by size:
937 182 171 168 162 138 99
MspA1I CmG'CkG
Cuts at: 0 13 573 702 915 1176 1263 1857
Size: 13 560 129 213 261 87 594
Fragments arranged by size:
594 560 261 213 129 87 13
NaeI GCC'GGC
Cuts at: 0 216 1857
Size: 216 1641
NcoI C'CATG_G
Cuts at: 0 362 926 1337 1857
Size: 362 564 411 520
Fragments arranged by size:
564 520 411 362
NgoMIV G'CCGG_C
Cuts at: 0 214 1857
Size: 214 1643
NsiI A_TGCA'T
Cuts at: 0 1531 1857
Size: 1531 326
NspI r_CATG'y
Cuts at: 0 1077 1779 1857
Size: 1077 702 78
Fragments arranged by size:
1077 702 78
PspOMI G'GGCC_C
Cuts at: 0 1816 1857
Size: 1816 41
PspXI vC'TCGA_Gb
Cuts at: 0 322 1857
Size: 322 1535
PvuII CAG'CTG
Cuts at: 0 702 1176 1857
Size: 702 474 681
Fragments arranged by size:
702 681 474
SacI G_AGCT'C
Cuts at: 0 248 974 1857
Size: 248 726 883
Fragments arranged by size:
883 726 248
SacII CC_GC'GG
Cuts at: 0 14 574 916 1857
Size: 14 560 342 941
Fragments arranged by size:
941 560 342 14
SapI GCTCTTCn'nnn_
Cuts at: 0 103 994 1857
Size: 103 891 863
Fragments arranged by size:
891 863 103
ScaI AGT'ACT
Cuts at: 0 661 1857
Size: 661 1196
SexAI A'CCwGG_T
Cuts at: 0 1328 1857
Size: 1328 529
SfcI C'TryA_G
Cuts at: 0 954 1791 1857
Size: 954 837 66
Fragments arranged by size:
954 837 66
SmlI C'TyrA_G
Cuts at: 0 322 973 1507 1857
Size: 322 651 534 350
Fragments arranged by size:
651 534 350 322
SphI G_CATG'C
Cuts at: 0 1077 1779 1857
Size: 1077 702 78
Fragments arranged by size:
1077 702 78
StuI AGG'CCT
Cuts at: 0 757 1594 1857
Size: 757 837 263
Fragments arranged by size:
837 757 263
StyI C'CwwG_G
Cuts at: 0 362 926 1337 1857
Size: 362 564 411 520
Fragments arranged by size:
564 520 411 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 562 833 1447 1857
Size: 562 271 614 410
Fragments arranged by size:
614 562 410 271
TatI w'GTAC_w
Cuts at: 0 659 1857
Size: 659 1198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1318 1350 1857
Size: 1318 32 507
Fragments arranged by size:
1318 507 32
XhoI C'TCGA_G
Cuts at: 0 322 1857
Size: 322 1535
XmnI GAAnn'nnTTC
Cuts at: 0 988 1857
Size: 988 869
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BbvCI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII
HpaI KpnI MluI MscI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PspOMI PspXI PvuII SacI SacII
SapI ScaI SexAI SfcI SmlI SphI StuI StyI
TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BclI BglII BmtI BsaI BsaBI BsaWI BsiWI
BspEI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI
DrdI EcoRI EcoRV FseI FspAI HindIII KasI MfeI
MmeI NarI NdeI NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI RsrII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XcmI XmaI ZraI