Restriction map for
pFN21AB0913
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AluI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BanII BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DdeI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MspA1I MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 264 GTAC GT^AC
AlwI 198 GATCC GGATC(4/5)
AspHI 223 GAGCAC G[AT]GC[AT]^C
AvaI 164 CCCGGG C^[CT]CG[GA]G
BanI 43 GGCGCC G^G[CT][GA]CC
BbeI 43 GGCGCC GGCGC^C
BbsI 181 GTCTTC GAAGAC(2/6)
BbvI 66 GCTGC GCAGC(8/12)
BmyI 223 GAGCAC G[AGT]GC[ACT]^C
BpmI 187 CTGGAG CTGGAG(16/14)
BpuAI 181 GTCTTC GAAGAC(2/6)
BsaWI 56 ACCGGA [AT]^CCGG[AT]
BseRI 157 GAGGAG GAGGAG(10/8)
BsiHKAI 223 GAGCAC G[AT]GC[AT]^C
BsmBI 109 CGTCTC CGTCTC(1/5)
BsmFI 71 GGGAC GGGAC(10/14)
BsoBI 164 CCCGGG C^[CT]CG[GA]G
Bsp1286I 223 GAGCAC G[AGT]GC[ACT]^C
BsrFI 96 GCCGGC [GA]^CCGG[CT]
BstF5I 255 GGATG GGATG(2/0)
BstXI 251 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 96 GCCGGC [GA]^CCGG[CT]
DpnII 198 GATC ^GATC
DsaI 251 CCATGG C^C[GA][CT]GG
EaeI 248 CGGCCA [CT]^GGCC[GA]
FauI 69 GCGGG CCCGC(4/6)
Fnu4HI 66 GCTGC GC^[ACGT]GC
FokI 255 GGATG GGATG(9/13)
HaeIII 249 GGCC GG^CC
HphI 210 GGTGA GGTGA(8/7)
ItaI 66 GCTGC GC^[ACGT]GC
KasI 43 GGCGCC G^GCGCC
MboI 198 GATC ^GATC
MboII 182 TCTTC GAAGA(8/7)
NaeI 96 GCCGGC GCC^GGC
NarI 43 GGCGCC GG^CGCC
NcoI 251 CCATGG C^CATGG
NdeII 198 GATC ^GATC
NgoMIV 96 GCCGGC G^CCGGC
NlaIII 252 CATG CATG^
NlaIV 43 GGCGCC GG[ACGT]^[ACGT]CC
PleI 170 GAGTC GAGTC(4/5)
RsaI 264 GTAC GT^AC
Sau3AI 198 GATC ^GATC
SfoI 43 GGCGCC GGC^GCC
SmaI 164 CCCGGG CCC^GGG
TfiI 102 GATTC G^A[AT]TC
TseI 66 GCTGC G^C[AT]GC
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 207 CGCG CG^CG
267 CGCG
AcyI 43 GGCGCC G[GA]^CG[CT]C
127 GGCGTC
BcnI 164 CCCGG CC^[GC]GG
165 CCGGG
BsaHI 43 GGCGCC G[GA]^CG[CT]C
127 GGCGTC
BsaI 160 GAGACC GGTCTC(1/5)
145 GGTCTC
BstNI 186 CCTGG CC^[AT]GG
279 CCAGG
BstUI 207 CGCG CG^CG
267 CGCG
Cac8I 96 GCCGGC GC[ACGT]^[ACGT]GC
225 GCACGC
EcoRII 186 CCTGG ^CC[AT]GG
279 CCAGG
EcoT14I 81 CCAAGG C^C[AT][AT]GG
251 CCATGG
HaeII 43 GGCGCC [GA]GCGC^[CT]
50 AGCGCC
HgaI 205 GACGC GACGC(5/10)
128 GCGTC
Hin1I 43 GGCGCC G[GA]^CG[CT]C
127 GGCGTC
HinfI 102 GATTC G^A[ACGT]TC
170 GAGTC
MaeII 120 ACGT A^CGT
194 ACGT
MslI 246 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
252 CATGGATGTG
MvaI 186 CCTGG CC^[AT]GG
279 CCAGG
MvnI 207 CGCG CG^CG
267 CGCG
NciI 164 CCCGG CC^[GC]GG
165 CCGGG
StyI 81 CCAAGG C^C[AT][AT]GG
251 CCATGG
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BsmAI
BssKI CfoI HapII HhaI HinP1I
HpaII MaeIII MnlI MspI MwoI
ScrFI Tsp45I