(Circular) (Six-base) MAPSORT of: pFN21AE0632.seq Check: 2381 from: 1 to: 5833 pFN21AE0632 5833 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2907 3643 278 Size: 53 83 186 936 1371 736 2468 Fragments arranged by size: 2468 1371 936 736 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 2599 3154 2599 Size: 555 5278 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3896 5280 5548 1632 Size: 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2645 1184 Size: 1461 4372 AlwNI CAG_nnn'CTG Cuts at: 3007 3046 3576 5148 3007 Size: 39 530 1572 3692 Fragments arranged by size: 3692 1572 530 39 ApaI G_GGCC'C Cuts at: 3120 3120 Size: 5833 ApaLI G'TGCA_C Cuts at: 2153 2204 2391 3890 5046 2153 Size: 51 187 1499 1156 2940 Fragments arranged by size: 2940 1499 1156 187 51 ApoI r'AATT_y Cuts at: 1493 2087 3142 3368 3520 5629 1493 Size: 594 1055 226 152 2109 1697 Fragments arranged by size: 2109 1697 1055 594 226 152 AscI GG'CGCG_CC Cuts at: 2955 2955 Size: 5833 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2462 2603 2800 2950 2976 3158 1870 Size: 73 519 141 197 150 26 182 4545 Fragments arranged by size: 4545 519 197 182 150 141 73 26 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 2572 2599 2819 3154 Size: 325 217 627 747 38 27 220 335 Cuts at: 3154 4609 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 747 627 335 325 220 217 38 27 BanII G_rGCy'C Cuts at: 1391 2333 3120 3152 1391 Size: 942 787 32 4072 Fragments arranged by size: 4072 942 787 32 BbeI G_GCGC'C Cuts at: 2538 2538 Size: 5833 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2275 2690 928 Size: 971 376 415 4071 Fragments arranged by size: 4071 971 415 376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4024 4058 1903 Size: 34 2087 34 3678 Fragments arranged by size: 3678 2087 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 3050 3250 3050 Size: 200 5633 Bme1580I G_kGCm'C Cuts at: 1790 2157 2208 2395 2577 2921 3120 3894 5050 Size: 367 51 187 182 344 199 774 1156 Cuts at: 5050 1790 Size: 2573 Fragments arranged by size: 2573 1156 774 367 344 199 187 182 51 BmgBI CAC'GTC Cuts at: 1318 2936 1318 Size: 1618 4215 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 2675 2945 3023 4493 1239 Size: 1346 90 270 78 1470 2579 Fragments arranged by size: 2579 1470 1346 270 90 78 Bpu10I CC'TnA_GC Cuts at: 2276 2276 Size: 5833 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3958 4823 5121 3958 Size: 865 298 4670 Fragments arranged by size: 4670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 4496 882 Size: 617 706 2291 2219 Fragments arranged by size: 2291 2219 706 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2535 2904 3640 4022 Size: 53 83 186 936 1002 369 736 382 Cuts at: 4022 4657 275 Size: 635 1451 Fragments arranged by size: 1451 1002 936 736 635 382 369 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2153 2183 1178 Size: 30 165 30 750 30 4828 Fragments arranged by size: 4828 750 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2315 3122 1186 Size: 1129 807 3897 Fragments arranged by size: 3897 1129 807 BseYI C'CCAG_C Cuts at: 2356 2512 5036 5532 2356 Size: 156 2524 496 2657 Fragments arranged by size: 2657 2524 496 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2359 2471 2916 1163 Size: 573 623 112 445 4080 Fragments arranged by size: 4080 623 573 445 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2785 3283 4044 5072 1540 Size: 275 186 784 498 761 1028 2301 Fragments arranged by size: 2301 1028 784 761 498 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 2208 2395 2624 3114 3894 3979 5050 2157 Size: 51 187 229 490 780 85 1071 2940 Fragments arranged by size: 2940 1071 780 490 229 187 85 51 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 BsmBI CGTCTCn'nnnn_ Cuts at: 2459 2459 Size: 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2208 2333 2395 2577 2624 2921 Size: 399 367 51 125 62 182 47 297 Cuts at: 2921 3114 3120 3152 3894 3979 5050 1391 Size: 193 6 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 367 297 193 182 125 85 62 51 47 32 6 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 3188 844 Size: 1379 965 3489 Fragments arranged by size: 3489 1379 965 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2472 2490 4477 4664 1700 Size: 110 662 18 1987 187 2869 Fragments arranged by size: 2869 1987 662 187 110 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 2361 2955 1925 Size: 436 594 4803 Fragments arranged by size: 4803 594 436 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 3007 794 Size: 2213 3620 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828 Size: 1191 367 385 17 1452 86 12 346 Cuts at: 5828 1972 Size: 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2823 3120 513 Size: 552 1758 297 3226 Fragments arranged by size: 3226 1758 552 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2544 2904 487 Size: 683 56 1318 360 3416 Fragments arranged by size: 3416 1318 683 360 56 BtsI GCAGTG_nn' Cuts at: 2064 3349 4143 4163 2064 Size: 1285 794 20 3734 Fragments arranged by size: 3734 1285 794 20 ClaI AT'CG_AT Cuts at: 1554 2445 1554 Size: 891 4942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2903 4840 809 Size: 507 1587 1937 1802 Fragments arranged by size: 1937 1802 1587 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2676 2782 3280 Size: 54 1425 50 275 18 846 106 498 Cuts at: 3280 4169 5798 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 846 498 275 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2782 3280 1537 Size: 275 970 498 4090 Fragments arranged by size: 4090 970 498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3763 1353 Size: 2410 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 2359 2414 2630 2897 4420 Size: 1064 138 510 260 55 216 267 1523 Cuts at: 4420 4794 4940 387 Size: 374 146 1280 Fragments arranged by size: 1523 1280 1064 510 374 267 260 216 146 138 55 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 2675 2945 3023 3896 4493 5280 Size: 393 953 90 270 78 873 597 787 Cuts at: 5280 5548 1239 Size: 268 1524 Fragments arranged by size: 1524 953 873 787 597 393 270 268 90 78 EcoO109I rG'GnC_Cy Cuts at: 2378 2608 2650 2837 3016 2378 Size: 230 42 187 179 5195 Fragments arranged by size: 5195 230 187 179 42 EcoRI G'AATT_C Cuts at: 2087 3142 3520 2087 Size: 1055 378 4400 Fragments arranged by size: 4400 1055 378 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 2357 2538 3089 4980 1678 Size: 679 181 551 1891 2531 Fragments arranged by size: 2531 1891 679 551 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4474 4506 4548 4580 1373 Size: 32 135 32 2902 32 42 32 2626 Fragments arranged by size: 2902 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KasI G'GCGC_C Cuts at: 2534 2534 Size: 5833 KpnI G_GTAC'C Cuts at: 2603 3158 2603 Size: 555 5278 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 4947 5131 1305 Size: 891 2751 184 2007 Fragments arranged by size: 2751 2007 891 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 2672 2705 3791 4150 4309 518 Size: 648 1281 225 33 1086 359 159 2042 Fragments arranged by size: 2042 1281 1086 648 359 225 159 33 MspA1I CmG'CkG Cuts at: 1606 2512 2825 3004 3013 3043 3122 3249 3926 Size: 906 313 179 9 30 79 127 677 Cuts at: 3926 5074 5319 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 906 677 313 245 179 127 79 30 9 NarI GG'CG_CC Cuts at: 2535 2535 Size: 5833 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5281 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 2782 3280 2782 Size: 498 5335 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PfoI T'CCnGG_A Cuts at: 2652 2652 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2378 2650 2837 3016 2378 Size: 272 187 179 5195 Fragments arranged by size: 5195 272 187 179 PshAI GACnn'nnGTC Cuts at: 1613 3072 1613 Size: 1459 4374 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PspOMI G'GGCC_C Cuts at: 3116 3116 Size: 5833 PstI C_TGCA'G Cuts at: 830 2862 3012 3185 830 Size: 2032 150 173 3478 Fragments arranged by size: 3478 2032 173 150 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 SacII CC_GC'GG Cuts at: 2826 3123 2826 Size: 297 5536 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SanDI GG'GwC_CC Cuts at: 2837 2837 Size: 5833 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SexAI A'CCwGG_T Cuts at: 2454 2694 2454 Size: 240 5593 SfcI C'TryA_G Cuts at: 826 1045 2858 3008 3181 4316 4997 5188 826 Size: 219 1813 150 173 1135 681 191 1471 Fragments arranged by size: 1813 1471 1135 681 219 191 173 150 SfoI GGC'GCC Cuts at: 2536 2536 Size: 5833 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 2472 1700 Size: 772 5061 SmaI CCC'GGG Cuts at: 1872 2605 2952 2978 3160 1872 Size: 733 347 26 182 4545 Fragments arranged by size: 4545 733 347 182 26 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4838 5100 820 Size: 197 926 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 3272 513 Size: 552 2207 3074 Fragments arranged by size: 3074 2207 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 3860 3877 4030 4215 4660 325 Size: 1255 594 1686 17 153 185 445 1498 Fragments arranged by size: 1686 1498 1255 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4079 5791 96 Size: 274 80 33 51 1443 2102 1712 138 Fragments arranged by size: 2102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2153 2185 5793 5825 2153 Size: 32 3608 32 2161 Fragments arranged by size: 3608 2161 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2456 2648 2696 2906 1316 Size: 1140 192 48 210 4243 Fragments arranged by size: 4243 1140 210 192 48 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 2446 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 2603 2950 2976 3158 1870 Size: 733 347 26 182 4545 Fragments arranged by size: 4545 733 347 182 26 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2905 3641 276 Size: 53 83 186 936 1371 736 2468 Fragments arranged by size: 2468 1371 936 736 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SfiI SrfI SwaI