Restriction map for pFN21AE0632
 (Linear) (Six-base) MAPSORT of: KIEE0632.seq  Check: 3132  from: 1  to: 1128



KIEE0632.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AatII G_ACGT'C

Cuts at:      0     902    1128 
   Size:        902     226
 
Acc65I G'GTAC_C

Cuts at:      0     594    1128 
   Size:        594     534
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     640    1128 
   Size:        640     488
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1002    1041    1128 
   Size:       1002      39      87

  Fragments arranged by size: 

                1002      87      39
 
ApaI G_GGCC'C

Cuts at:      0    1115    1128 
   Size:       1115      13
 
ApaLI G'TGCA_C

Cuts at:      0     148     199     386    1128 
   Size:        148      51     187     742

  Fragments arranged by size: 

                 742     187     148      51
 
ApoI r'AATT_y

Cuts at:      0      82    1128 
   Size:         82    1046
 
AscI GG'CGCG_CC

Cuts at:      0     950    1128 
   Size:        950     178
 
AvaI C'yCGr_G

Cuts at:      0     457     598     795     945     971    1128 
   Size:        457     141     197     150      26     157

  Fragments arranged by size: 

                 457     197     157     150     141      26
 
BanI G'GyrC_C

Cuts at:      0     529     567     594     814    1128 
   Size:        529      38      27     220     314

  Fragments arranged by size: 

                 529     314     220      38      27
 
BanII G_rGCy'C

Cuts at:      0     328    1115    1128 
   Size:        328     787      13

  Fragments arranged by size: 

                 787     328      13
 
BbeI G_GCGC'C

Cuts at:      0     533    1128 
   Size:        533     595
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     270     685    1128 
   Size:        270     415     443

  Fragments arranged by size: 

                 443     415     270
 
BlpI GC'TnA_GC

Cuts at:      0    1045    1128 
   Size:       1045      83
 
Bme1580I G_kGCm'C

Cuts at:      0     152     203     390     572     916    1115    1128 
   Size:        152      51     187     182     344     199      13

  Fragments arranged by size: 

                 344     199     187     182     152      51      13
 
BmgBI CAC'GTC

Cuts at:      0     931    1128 
   Size:        931     197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     670     940    1018    1128 
   Size:        580      90     270      78     110

  Fragments arranged by size: 

                 580     270     110      90      78
 
Bpu10I CC'TnA_GC

Cuts at:      0     271    1128 
   Size:        271     857
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     200    1128 
   Size:        200     928
 
BsaHI Gr'CG_yC

Cuts at:      0     530     899    1128 
   Size:        530     369     229

  Fragments arranged by size: 

                 530     369     229
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     148     178    1128 
   Size:        148      30     950

  Fragments arranged by size: 

                 950     148      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     310    1117    1128 
   Size:        310     807      11

  Fragments arranged by size: 

                 807     310      11
 
BseYI C'CCAG_C

Cuts at:      0     351     507    1128 
   Size:        351     156     621

  Fragments arranged by size: 

                 621     351     156
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     466     911    1128 
   Size:        354     112     445     217

  Fragments arranged by size: 

                 445     354     217     112
 
BsiEI CG_ry'CG

Cuts at:      0     780    1128 
   Size:        780     348
 
BsiHKAI G_wGCw'C

Cuts at:      0     152     203     390     619    1109    1128 
   Size:        152      51     187     229     490      19

  Fragments arranged by size: 

                 490     229     187     152      51      19
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     454    1128 
   Size:        454     674
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     203     328     390     572     619     916    1109 
   Size:        152      51     125      62     182      47     297     193

Cuts at:   1109    1115    1128 
   Size:          6      13

  Fragments arranged by size: 

                 297     193     182     152     125      62      51      47
                  13       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     218    1128 
   Size:        218     910
 
BsrFI r'CCGG_y

Cuts at:      0     467     485    1128 
   Size:        467      18     643

  Fragments arranged by size: 

                 643     467      18
 
BssHII G'CGCG_C

Cuts at:      0     356     950    1128 
   Size:        356     594     178

  Fragments arranged by size: 

                 594     356     178
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1002    1128 
   Size:       1002     126
 
BtgI C'CryG_G

Cuts at:      0     818    1115    1128 
   Size:        818     297      13

  Fragments arranged by size: 

                 818     297      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     539     899    1128 
   Size:        539     360     229

  Fragments arranged by size: 

                 539     360     229
 
BtsI GCAGTG_nn'

Cuts at:      0      59    1128 
   Size:         59    1069
 
ClaI AT'CG_AT

Cuts at:      0     440    1128 
   Size:        440     688
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     898    1128 
   Size:        898     230
 
EaeI y'GGCC_r

Cuts at:      0     671     777    1128 
   Size:        671     106     351

  Fragments arranged by size: 

                 671     351     106
 
EagI C'GGCC_G

Cuts at:      0     777    1128 
   Size:        777     351
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      94     354     409     625     892    1128 
   Size:         94     260      55     216     267     236

  Fragments arranged by size: 

                 267     260     236     216      94      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     670     940    1018    1128 
   Size:        580      90     270      78     110

  Fragments arranged by size: 

                 580     270     110      90      78
 
EcoO109I rG'GnC_Cy

Cuts at:      0     373     603     645     832    1011    1128 
   Size:        373     230      42     187     179     117

  Fragments arranged by size: 

                 373     230     187     179     117      42
 
EcoRI G'AATT_C

Cuts at:      0      82    1128 
   Size:         82    1046
 
HaeII r_GCGC'y

Cuts at:      0     352     533    1084    1128 
   Size:        352     181     551      44

  Fragments arranged by size: 

                 551     352     181      44
 
KasI G'GCGC_C

Cuts at:      0     529    1128 
   Size:        529     599
 
KpnI G_GTAC'C

Cuts at:      0     598    1128 
   Size:        598     530
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1128 
   Size:        191     937
 
MslI CAynn'nnrTG

Cuts at:      0     442     667     700    1128 
   Size:        442     225      33     428

  Fragments arranged by size: 

                 442     428     225      33
 
MspA1I CmG'CkG

Cuts at:      0     507     820     999    1008    1038    1117    1128 
   Size:        507     313     179       9      30      79      11

  Fragments arranged by size: 

                 507     313     179      79      30      11       9
 
NarI GG'CG_CC

Cuts at:      0     530    1128 
   Size:        530     598
 
NotI GC'GGCC_GC

Cuts at:      0     777    1128 
   Size:        777     351
 
PfoI T'CCnGG_A

Cuts at:      0     647    1128 
   Size:        647     481
 
PpuMI rG'GwC_Cy

Cuts at:      0     373     645     832    1011    1128 
   Size:        373     272     187     179     117

  Fragments arranged by size: 

                 373     272     187     179     117
 
PshAI GACnn'nnGTC

Cuts at:      0    1067    1128 
   Size:       1067      61
 
PspOMI G'GGCC_C

Cuts at:      0    1111    1128 
   Size:       1111      17
 
PstI C_TGCA'G

Cuts at:      0     857    1007    1128 
   Size:        857     150     121

  Fragments arranged by size: 

                 857     150     121
 
SacII CC_GC'GG

Cuts at:      0     821    1118    1128 
   Size:        821     297      10

  Fragments arranged by size: 

                 821     297      10
 
SanDI GG'GwC_CC

Cuts at:      0     832    1128 
   Size:        832     296
 
SexAI A'CCwGG_T

Cuts at:      0     449     689    1128 
   Size:        449     240     439

  Fragments arranged by size: 

                 449     439     240
 
SfcI C'TryA_G

Cuts at:      0     853    1003    1128 
   Size:        853     150     125

  Fragments arranged by size: 

                 853     150     125
 
SfoI GGC'GCC

Cuts at:      0     531    1128 
   Size:        531     597
 
SgrAI Cr'CCGG_yG

Cuts at:      0     467    1128 
   Size:        467     661
 
SmaI CCC'GGG

Cuts at:      0     600     947     973    1128 
   Size:        600     347      26     155

  Fragments arranged by size: 

                 600     347     155      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     169    1128 
   Size:        169     959
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     148     180    1128 
   Size:        148      32     948

  Fragments arranged by size: 

                 948     148      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     451     643     691     901    1128 
   Size:        451     192      48     210     227

  Fragments arranged by size: 

                 451     227     210     192      48
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     441    1128 
   Size:        441     687
 
XmaI C'CCGG_G

Cuts at:      0     598     945     971    1128 
   Size:        598     347      26     157

  Fragments arranged by size: 

                 598     347     157      26
 
ZraI GAC'GTC

Cuts at:      0     900    1128 
   Size:        900     228
 

 Enzymes that do cut:

    AatII   Acc65I     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AvaI     BanI    BanII     BbeI     BbsI     BlpI Bme1580I    BmgBI
     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI   BssHII   BstAPI
     BtgI    BtgZI     BtsI     ClaI     DrdI     EaeI     EagI     EciI
  Eco57MI EcoO109I    EcoRI    HaeII     KasI     KpnI     MmeI     MslI
   MspA1I     NarI     NotI     PfoI    PpuMI    PshAI   PspOMI     PstI
    SacII    SanDI    SexAI     SfcI     SfoI    SgrAI     SmaI    TaqII
     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    CspCI     DraI   DraIII     EarI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     MfeI     MluI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     XbaI     XhoI     XmnI
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