Restriction map for pFN21AB8471
 (Linear) (Six-base) MAPSORT of: KIBB8471.seq  Check: 8818  from: 1  to: 2736



KIBB8471.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1110    1655    2736 
   Size:       1110     545    1081

  Fragments arranged by size: 

                1110    1081     545
 
AatII G_ACGT'C

Cuts at:      0    2060    2630    2736 
   Size:       2060     570     106

  Fragments arranged by size: 

                2060     570     106
 
Acc65I G'GTAC_C

Cuts at:      0    1768    2736 
   Size:       1768     968
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     986    1969    2734    2736 
   Size:        398     588     983     765       2

  Fragments arranged by size: 

                 983     765     588     398       2
 
AflIII A'CryG_T

Cuts at:      0    1313    1751    2736 
   Size:       1313     438     985

  Fragments arranged by size: 

                1313     985     438
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2126    2661    2736 
   Size:       2126     535      75

  Fragments arranged by size: 

                2126     535      75
 
ApaI G_GGCC'C

Cuts at:      0     342     698    1230    2098    2373    2736 
   Size:        342     356     532     868     275     363

  Fragments arranged by size: 

                 868     532     363     356     342     275
 
ApaLI G'TGCA_C

Cuts at:      0    2580    2736 
   Size:       2580     156
 
ApoI r'AATT_y

Cuts at:      0    1003    1846    2736 
   Size:       1003     843     890

  Fragments arranged by size: 

                1003     890     843
 
AvaI C'yCGr_G

Cuts at:      0      25     497     697    2317    2736 
   Size:         25     472     200    1620     419

  Fragments arranged by size: 

                1620     472     419     200      25
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1752    1785    2736 
   Size:       1752      33     951

  Fragments arranged by size: 

                1752     951      33
 
BanI G'GyrC_C

Cuts at:      0     247     970    1768    2027    2736 
   Size:        247     723     798     259     709

  Fragments arranged by size: 

                 798     723     709     259     247
 
BanII G_rGCy'C

Cuts at:      0     122     296     316     342     435     496     698     791 
   Size:        122     174      20      26      93      61     202      93

Cuts at:    791    1045    1230    1940    2098    2373    2736 
   Size:        254     185     710     158     275     363

  Fragments arranged by size: 

                 710     363     275     254     202     185     174     158
                 122      93      93      61      26      20
 
BbeI G_GCGC'C

Cuts at:      0     251    2736 
   Size:        251    2485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1739    2541    2736 
   Size:       1739     802     195

  Fragments arranged by size: 

                1739     802     195
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1259    1809    2736 
   Size:       1259     550     927

  Fragments arranged by size: 

                1259     927     550
 
BlpI GC'TnA_GC

Cuts at:      0    1039    2736 
   Size:       1039    1697
 
Bme1580I G_kGCm'C

Cuts at:      0     342     698    1230    1763    2098    2179    2373    2584 
   Size:        342     356     532     533     335      81     194     211

Cuts at:   2584    2736 
   Size:        152

  Fragments arranged by size: 

                 533     532     356     342     335     211     194     152
                  81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2605    2736 
   Size:       2605     131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1285    1585    1798    2458    2476    2736 
   Size:       1285     300     213     660      18     260

  Fragments arranged by size: 

                1285     660     300     260     213      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     785    2736 
   Size:        785    1951
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369    2736 
   Size:        369    2367
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     361     457    2736 
   Size:        361      96    2279

  Fragments arranged by size: 

                2279     361      96
 
BsaAI yAC'GTr

Cuts at:      0    1073    2736 
   Size:       1073    1663
 
BsaHI Gr'CG_yC

Cuts at:      0     248    2057    2627    2736 
   Size:        248    1809     570     109

  Fragments arranged by size: 

                1809     570     248     109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     871     901    1558    1588    1789    1819    2736 
   Size:        871      30     657      30     201      30     917

  Fragments arranged by size: 

                 917     871     657     201      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73      94     103     121     319     443     733    2736 
   Size:         73      21       9      18     198     124     290    2003

  Fragments arranged by size: 

                2003     290     198     124      73      21      18       9
            
 
BseYI C'CCAG_C

Cuts at:      0     121     296     319     342     524     805     977    1878 
   Size:        121     175      23      23     182     281     172     901

Cuts at:   1878    2664    2736 
   Size:        786      72

  Fragments arranged by size: 

                 901     786     281     182     175     172     121      72
                  23      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380     393     760    1222    1593    2345    2736 
   Size:        380      13     367     462     371     752     391

  Fragments arranged by size: 

                 752     462     391     380     371     367      13
 
BsiEI CG_ry'CG

Cuts at:      0     861    2736 
   Size:        861    1875
 
BsiHKAI G_wGCw'C

Cuts at:      0    1045    1381    1640    2584    2736 
   Size:       1045     336     259     944     152

  Fragments arranged by size: 

                1045     944     336     259     152
 
BsmI GAATG_Cn'

Cuts at:      0     678     822    1961    2736 
   Size:        678     144    1139     775

  Fragments arranged by size: 

                1139     775     678     144
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     989    2634    2736 
   Size:        989    1645     102

  Fragments arranged by size: 

                1645     989     102
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     296     316     342     435     496     698     791 
   Size:        122     174      20      26      93      61     202      93

Cuts at:    791    1045    1230    1381    1640    1763    1940    2098    2179 
   Size:        254     185     151     259     123     177     158      81

Cuts at:   2179    2373    2584    2736 
   Size:        194     211     152

  Fragments arranged by size: 

                 259     254     211     202     194     185     177     174
                 158     152     151     123     122      93      93      81
                  61      26      20
 
BspHI T'CATG_A

Cuts at:      0    2552    2736 
   Size:       2552     184
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1110    1655    2736 
   Size:       1110     545    1081

  Fragments arranged by size: 

                1110    1081     545
 
BsrBI CCG'CTC

Cuts at:      0      54    1431    2736 
   Size:         54    1377    1305

  Fragments arranged by size: 

                1377    1305      54
 
BsrDI GCAATG_nn'

Cuts at:      0     659    1979    2136    2736 
   Size:        659    1320     157     600

  Fragments arranged by size: 

                1320     659     600     157
 
BsrFI r'CCGG_y

Cuts at:      0     503    2183    2669    2736 
   Size:        503    1680     486      67

  Fragments arranged by size: 

                1680     503     486      67
 
BsrGI T'GTAC_A

Cuts at:      0    1304    2736 
   Size:       1304    1432
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1556    1616    2126    2661    2736 
   Size:       1556      60     510     535      75

  Fragments arranged by size: 

                1556     535     510      75      60
 
BstEII G'GTnAC_C

Cuts at:      0    2386    2736 
   Size:       2386     350
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     486    1194    1249    2013    2736 
   Size:        486     708      55     764     723

  Fragments arranged by size: 

                 764     723     708     486      55
 
Bsu36I CC'TnA_GG

Cuts at:      0    1256    1328    2736 
   Size:       1256      72    1408

  Fragments arranged by size: 

                1408    1256      72
 
BtgI C'CryG_G

Cuts at:      0     128     230     791    1193    2279    2736 
   Size:        128     102     561     402    1086     457

  Fragments arranged by size: 

                1086     561     457     402     128     102
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     848    1184    2736 
   Size:        848     336    1552

  Fragments arranged by size: 

                1552     848     336
 
BtsI GCAGTG_nn'

Cuts at:      0     398    1059    1181    2736 
   Size:        398     661     122    1555

  Fragments arranged by size: 

                1555     661     398     122
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     374     409    2736 
   Size:        374      35    2327

  Fragments arranged by size: 

                2327     374      35
 
EaeI y'GGCC_r

Cuts at:      0     858     916    1124    2181    2276    2736 
   Size:        858      58     208    1057      95     460

  Fragments arranged by size: 

                1057     858     460     208      95      58
 
EagI C'GGCC_G

Cuts at:      0     858    2736 
   Size:        858    1878
 
EarI CTCTTCn'nnn_

Cuts at:      0    1274    1369    1528    1882    2194    2707    2736 
   Size:       1274      95     159     354     312     513      29

  Fragments arranged by size: 

                1274     513     354     312     159      95      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2683    2736 
   Size:       2683      53
 
EcoICRI GAG'CTC

Cuts at:      0    1043    2736 
   Size:       1043    1693
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     986    1285    1585    1798    1969    2458    2476 
   Size:        398     588     299     300     213     171     489      18

Cuts at:   2476    2734    2736 
   Size:        258       2

  Fragments arranged by size: 

                 588     489     398     300     299     258     213     171
                  18       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2170    2736 
   Size:       2170     566
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201     338     339     579     694    1113    1227    1260 
   Size:        201     137       1     240     115     419     114      33

Cuts at:   1260    2094    2163    2369    2736 
   Size:        834      69     206     367

  Fragments arranged by size: 

                 834     419     367     240     206     201     137     115
                 114      69      33       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1575    2736 
   Size:       1575    1161
 
HaeII r_GCGC'y

Cuts at:      0     114     251    2736 
   Size:        114     137    2485

  Fragments arranged by size: 

                2485     137     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     745     777    1789    1821    2736 
   Size:        745      32    1012      32     915

  Fragments arranged by size: 

                1012     915     745      32      32
 
HincII GTy'rAC

Cuts at:      0    2148    2736 
   Size:       2148     588
 
KasI G'GCGC_C

Cuts at:      0     247    2736 
   Size:        247    2489
 
KpnI G_GTAC'C

Cuts at:      0    1772    2736 
   Size:       1772     964
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1509    2348    2657    2736 
   Size:       1509     839     309      79

  Fragments arranged by size: 

                1509     839     309      79
 
MscI TGG'CCA

Cuts at:      0     918    1126    2278    2736 
   Size:        918     208    1152     458

  Fragments arranged by size: 

                1152     918     458     208
 
MslI CAynn'nnrTG

Cuts at:      0     484    1312    1318    1903    1966    2011    2587    2736 
   Size:        484     828       6     585      63      45     576     149

  Fragments arranged by size: 

                 828     585     576     484     149      63      45       6
            
 
MspA1I CmG'CkG

Cuts at:      0      20     364     546     964     977    1038    1336    1384 
   Size:         20     344     182     418      13      61     298      48

Cuts at:   1384    1882    2359    2712    2736 
   Size:        498     477     353      24

  Fragments arranged by size: 

                 498     477     418     353     344     298     182      61
                  48      24      20      13
 
NaeI GCC'GGC

Cuts at:      0     505    2185    2736 
   Size:        505    1680     551

  Fragments arranged by size: 

                1680     551     505
 
NarI GG'CG_CC

Cuts at:      0     248    2736 
   Size:        248    2488
 
NcoI C'CATG_G

Cuts at:      0     230    2279    2736 
   Size:        230    2049     457

  Fragments arranged by size: 

                2049     457     230
 
NgoMIV G'CCGG_C

Cuts at:      0     503    2183    2736 
   Size:        503    1680     553

  Fragments arranged by size: 

                1680     553     503
 
NruI TCG'CGA

Cuts at:      0    2310    2736 
   Size:       2310     426
 
NspI r_CATG'y

Cuts at:      0    1317    1755    2736 
   Size:       1317     438     981

  Fragments arranged by size: 

                1317     981     438
 
PasI CC'CwG_GG

Cuts at:      0    1230    2736 
   Size:       1230    1506
 
PciI A'CATG_T

Cuts at:      0    1313    1751    2736 
   Size:       1313     438     985

  Fragments arranged by size: 

                1313     985     438
 
PfoI T'CCnGG_A

Cuts at:      0    2084    2736 
   Size:       2084     652
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1505    1537    2736 
   Size:       1505      32    1199

  Fragments arranged by size: 

                1505    1199      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     201     579    1113    2736 
   Size:        201     378     534    1623

  Fragments arranged by size: 

                1623     534     378     201
 
PspOMI G'GGCC_C

Cuts at:      0     338     694    1226    2094    2369    2736 
   Size:        338     356     532     868     275     367

  Fragments arranged by size: 

                 868     532     367     356     338     275
 
PstI C_TGCA'G

Cuts at:      0     441    1121    1666    2447    2736 
   Size:        441     680     545     781     289

  Fragments arranged by size: 

                 781     680     545     441     289
 
PvuII CAG'CTG

Cuts at:      0     364     964    1336    1882    2359    2736 
   Size:        364     600     372     546     477     377

  Fragments arranged by size: 

                 600     546     477     377     372     364
 
SacI G_AGCT'C

Cuts at:      0    1045    2736 
   Size:       1045    1691
 
SanDI GG'GwC_CC

Cuts at:      0     579    2736 
   Size:        579    2157
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1369    2194    2736 
   Size:       1369     825     542

  Fragments arranged by size: 

                1369     825     542
 
SbfI CC_TGCA'GG

Cuts at:      0    1121    2736 
   Size:       1121    1615
 
SfcI C'TryA_G

Cuts at:      0     437    1117    1662    1931    2443    2736 
   Size:        437     680     545     269     512     293

  Fragments arranged by size: 

                 680     545     512     437     293     269
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1259    2736 
   Size:       1259    1477
 
SfoI GGC'GCC

Cuts at:      0     249    2736 
   Size:        249    2487
 
SmaI CCC'GGG

Cuts at:      0      27     499     699    2736 
   Size:         27     472     200    2037

  Fragments arranged by size: 

                2037     472     200      27
 
SmlI C'TyrA_G

Cuts at:      0     384    2317    2736 
   Size:        384    1933     419

  Fragments arranged by size: 

                1933     419     384
 
StyI C'CwwG_G

Cuts at:      0     230     416     653    1187    1394    2228    2279    2736 
   Size:        230     186     237     534     207     834      51     457

  Fragments arranged by size: 

                 834     534     457     237     230     207     186      51
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      80    1308    2736 
   Size:         80    1228    1428

  Fragments arranged by size: 

                1428    1228      80
 
TatI w'GTAC_w

Cuts at:      0    1304    2736 
   Size:       1304    1432
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     871     903    2736 
   Size:        871      32    1833

  Fragments arranged by size: 

                1833     871      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     273    2736 
   Size:        273    2463
 
XhoI C'TCGA_G

Cuts at:      0    2317    2736 
   Size:       2317     419
 
XmaI C'CCGG_G

Cuts at:      0      25     497     697    2736 
   Size:         25     472     200    2039

  Fragments arranged by size: 

                2039     472     200      25
 
XmnI GAAnn'nnTTC

Cuts at:      0    1147    2736 
   Size:       1147    1589
 
ZraI GAC'GTC

Cuts at:      0    2058    2628    2736 
   Size:       2058     570     108

  Fragments arranged by size: 

                2058     570     108
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BaeI     BanI    BanII     BbeI     BbsI     BglI
     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BstAPI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI     NspI
     PasI     PciI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI     SbfI     SfcI     SfiI     SfoI     SmaI
     SmlI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI     BplI    BsaBI
    BsaWI    BsiWI    BspEI   BssHII    BssSI    BstBI    BstYI  BstZ17I
     ClaI     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI
     NsiI     PacI    PflMI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com