Restriction map for
pFN21AB9995
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AvaII AviII AvrII BamHI BbrPI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV FbaI
FseI FspI HpaI KpnI MamI
MfeI MroI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AocI 872 CCTTAGG CC^T[ACGT]AGG
Asp700I 369 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1209 GTGCTC G[AT]GC[AT]^C
AspI 1654 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 688 TGGCCA TGG^CCA
BbeI 727 GGCGCC GGCGC^C
BbsI 1549 GAAGAC GAAGAC(2/6)
BbvCI 1495 CCTCAGC CCTCAGC(-5/-2)
BcgI 79 CGAGCCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 527 CTAG C^TAG
BglI 202 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1073 AGATCT A^GATCT
BpuAI 1549 GAAGAC GAAGAC(2/6)
BsiHKAI 1209 GTGCTC G[AT]GC[AT]^C
BsmBI 149 GAGACG CGTCTC(1/5)
BspMI 287 ACCTGC ACCTGC(4/8)
BsrBI 171 GAGCGG CCGCTC(-3/-3)
BssSI 991 CTCGTG CACGAG(-5/-1)
BstXI 1604 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1073 AGATCT [GA]^GATC[CT]
Bsu36I 872 CCTTAGG CC^T[ACGT]AGG
DraI 1357 TTTAAA TTT^AAA
DsaI 190 CCGCGG C^C[GA][CT]GG
EaeI 688 TGGCCA [CT]^GGCC[GA]
Eco81I 872 CCTTAGG CC^T[ACGT]AGG
EcoNI 1575 CCTCTCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 727 GGCGCC [GA]GCGC^[CT]
HincII 259 GTCAAC GT[CT]^[GA]AC
HindII 259 GTCAAC GT[CT]^[GA]AC
HindIII 1533 AAGCTT A^AGCTT
KasI 727 GGCGCC G^GCGCC
KspI 190 CCGCGG CCGC^GG
MaeI 527 CTAG C^TAG
MflI 1073 AGATCT [GA]^GATC[CT]
MluI 266 ACGCGT A^CGCGT
MluNI 688 TGGCCA TGG^CCA
MscI 688 TGGCCA TGG^CCA
NarI 727 GGCGCC GG^CGCC
PflFI 1654 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1641 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 190 CCGCGG CCGC^GG
SapI 805 GCTCTTC GCTCTTC(1/4)
SfoI 727 GGCGCC GGC^GCC
SmlI 853 CTCAAG C^T[CT][GA]AG
SphI 1263 GCATGC GCATG^C
StuI 1325 AGGCCT AGG^CCT
Tth111I 1654 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1641 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1073 AGATCT [GA]^GATC[CT]
XmnI 369 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 462 AAATTT [GA]^AATT[CT]
473 AAATTT
ApoI 462 AAATTT [GA]^AATT[CT]
473 AAATTT
BanI 86 GGTGCC G^G[CT][GA]CC
727 GGCGCC
BanII 12 GGGCTC G[GA]GC[CT]^C
696 GGGCTC
BspLU11I 608 ACATGT A^CATGT
740 ACATGT
BsrDI 484 CATTGC GCAATG(2/0)
819 CATTGC
BsrFI 162 GCCGGC [GA]^CCGG[CT]
294 GCCGGC
Cfr10I 162 GCCGGC [GA]^CCGG[CT]
294 GCCGGC
EcoT14I 1646 CCTTGG C^C[AT][AT]GG
1740 CCTTGG
HgaI 151 GACGC GACGC(5/10)
1340 GACGC
MaeIII 655 GTCAC ^GT[ACGT]AC
1168 GTGAC
NaeI 162 GCCGGC GCC^GGC
294 GCCGGC
NgoMIV 162 GCCGGC G^CCGGC
294 GCCGGC
PleI 1196 GAGTC GAGTC(4/5)
1569 GAGTC
PstI 237 CTGCAG CTGCA^G
866 CTGCAG
PvuII 240 CAGCTG CAG^CTG
1004 CAGCTG
SfcI 237 CTGCAG C^T[GA][CT]AG
866 CTGCAG
StyI 1646 CCTTGG C^C[AT][AT]GG
1740 CCTTGG
TaqI 377 TCGA T^CGA
1579 TCGA
Tsp45I 655 GTCAC ^GT[GC]AC
1168 GTGAC
TthHB8I 377 TCGA T^CGA
1579 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI