Restriction map for pFN21AB9995
 (Linear) (Six-base) MAPSORT of: KIBB9995.seq  Check: 7913  from: 1  to: 1755



KIBB9995.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340    1755 
   Size:        340    1415
 
AflIII A'CryG_T

Cuts at:      0     266     608     740    1755 
   Size:        266     342     132    1015

  Fragments arranged by size: 

                1015     342     266     132
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1106    1229    1276    1755 
   Size:       1106     123      47     479

  Fragments arranged by size: 

                1106     479     123      47
 
ApoI r'AATT_y

Cuts at:      0     462     473    1755 
   Size:        462      11    1282

  Fragments arranged by size: 

                1282     462      11
 
BanI G'GyrC_C

Cuts at:      0      86     727    1755 
   Size:         86     641    1028

  Fragments arranged by size: 

                1028     641      86
 
BanII G_rGCy'C

Cuts at:      0      16     700    1755 
   Size:         16     684    1055

  Fragments arranged by size: 

                1055     684      16
 
BbeI G_GCGC'C

Cuts at:      0     731    1755 
   Size:        731    1024
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1556    1755 
   Size:       1556     199
 
BbvCI CC'TCA_GC

Cuts at:      0    1496    1755 
   Size:       1496     259
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      68     102    1755 
   Size:         68      34    1653

  Fragments arranged by size: 

                1653      68      34
 
BfrBI ATG'CAT

Cuts at:      0     483    1083    1263    1755 
   Size:        483     600     180     492

  Fragments arranged by size: 

                 600     492     483     180
 
BglI GCCn_nnn'nGGC

Cuts at:      0     208    1755 
   Size:        208    1547
 
BglII A'GATC_T

Cuts at:      0    1073    1755 
   Size:       1073     682
 
BmgBI CAC'GTC

Cuts at:      0     830    1172    1755 
   Size:        830     342     583

  Fragments arranged by size: 

                 830     583     342
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614    1337    1574    1755 
   Size:        614     723     237     181

  Fragments arranged by size: 

                 723     614     237     181
 
Bpu10I CC'TnA_GC

Cuts at:      0    1496    1755 
   Size:       1496     259
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838    1755 
   Size:        838     917
 
BsaHI Gr'CG_yC

Cuts at:      0     152     728    1341    1755 
   Size:        152     576     613     414

  Fragments arranged by size: 

                 613     576     414     152
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1643    1673    1755 
   Size:       1643      30      82

  Fragments arranged by size: 

                1643      82      30
 
BseYI C'CCAG_C

Cuts at:      0    1689    1755 
   Size:       1689      66
 
BsiHKAI G_wGCw'C

Cuts at:      0    1213    1755 
   Size:       1213     542
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     143    1755 
   Size:        143    1612
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     700    1213    1755 
   Size:         16     684     513     542

  Fragments arranged by size: 

                 684     542     513      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     296    1755 
   Size:        296    1459
 
BsrBI CCG'CTC

Cuts at:      0     173    1755 
   Size:        173    1582
 
BsrDI GCAATG_nn'

Cuts at:      0     483     818    1755 
   Size:        483     335     937

  Fragments arranged by size: 

                 937     483     335
 
BsrFI r'CCGG_y

Cuts at:      0     162     294    1755 
   Size:        162     132    1461

  Fragments arranged by size: 

                1461     162     132
 
BssSI C'ACGA_G

Cuts at:      0     991    1755 
   Size:        991     764
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1611    1755 
   Size:       1611     144
 
BstYI r'GATC_y

Cuts at:      0    1073    1755 
   Size:       1073     682
 
Bsu36I CC'TnA_GG

Cuts at:      0     873    1755 
   Size:        873     882
 
BtgI C'CryG_G

Cuts at:      0     190    1755 
   Size:        190    1565
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     719    1755 
   Size:        719    1036
 
BtsI GCAGTG_nn'

Cuts at:      0    1213    1755 
   Size:       1213     542
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     446     481    1755 
   Size:        446      35    1274

  Fragments arranged by size: 

                1274     446      35
 
DraI TTT'AAA

Cuts at:      0    1359    1755 
   Size:       1359     396
 
EaeI y'GGCC_r

Cuts at:      0     688    1755 
   Size:        688    1067
 
EarI CTCTTCn'nnn_

Cuts at:      0     812    1226    1553    1755 
   Size:        812     414     327     202

  Fragments arranged by size: 

                 812     414     327     202
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     181    1755 
   Size:        181    1574
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     614    1337    1574    1755 
   Size:        340     274     723     237     181

  Fragments arranged by size: 

                 723     340     274     237     181
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1579    1755 
   Size:       1579     176
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     335    1755 
   Size:        335    1420
 
HaeII r_GCGC'y

Cuts at:      0     731    1755 
   Size:        731    1024
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     702     734    1161    1193    1554    1586    1643    1675 
   Size:        702      32     427      32     361      32      57      32

Cuts at:   1675    1755 
   Size:         80

  Fragments arranged by size: 

                 702     427     361      80      57      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     261    1755 
   Size:        261    1494
 
HindIII A'AGCT_T

Cuts at:      0    1533    1755 
   Size:       1533     222
 
KasI G'GCGC_C

Cuts at:      0     727    1755 
   Size:        727    1028
 
MluI A'CGCG_T

Cuts at:      0     266    1755 
   Size:        266    1489
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     546    1755 
   Size:         17     529    1209

  Fragments arranged by size: 

                1209     529      17
 
MscI TGG'CCA

Cuts at:      0     690    1755 
   Size:        690    1065
 
MslI CAynn'nnrTG

Cuts at:      0    1088    1349    1505    1755 
   Size:       1088     261     156     250

  Fragments arranged by size: 

                1088     261     250     156
 
MspA1I CmG'CkG

Cuts at:      0     192     242    1006    1693    1755 
   Size:        192      50     764     687      62

  Fragments arranged by size: 

                 764     687     192      62      50
 
NaeI GCC'GGC

Cuts at:      0     164     296    1755 
   Size:        164     132    1459

  Fragments arranged by size: 

                1459     164     132
 
NarI GG'CG_CC

Cuts at:      0     728    1755 
   Size:        728    1027
 
NgoMIV G'CCGG_C

Cuts at:      0     162     294    1755 
   Size:        162     132    1461

  Fragments arranged by size: 

                1461     162     132
 
NsiI A_TGCA'T

Cuts at:      0     485    1085    1265    1755 
   Size:        485     600     180     490

  Fragments arranged by size: 

                 600     490     485     180
 
NspI r_CATG'y

Cuts at:      0     612     744    1087    1267    1411    1755 
   Size:        612     132     343     180     144     344

  Fragments arranged by size: 

                 612     344     343     180     144     132
 
PciI A'CATG_T

Cuts at:      0     608     740    1755 
   Size:        608     132    1015

  Fragments arranged by size: 

                1015     608     132
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1647    1755 
   Size:       1647     108
 
PstI C_TGCA'G

Cuts at:      0     241     870    1755 
   Size:        241     629     885

  Fragments arranged by size: 

                 885     629     241
 
PvuII CAG'CTG

Cuts at:      0     242    1006    1755 
   Size:        242     764     749

  Fragments arranged by size: 

                 764     749     242
 
SacII CC_GC'GG

Cuts at:      0     193    1755 
   Size:        193    1562
 
SapI GCTCTTCn'nnn_

Cuts at:      0     812    1755 
   Size:        812     943
 
SfcI C'TryA_G

Cuts at:      0     237     866    1755 
   Size:        237     629     889

  Fragments arranged by size: 

                 889     629     237
 
SfoI GGC'GCC

Cuts at:      0     729    1755 
   Size:        729    1026
 
SmlI C'TyrA_G

Cuts at:      0     853    1755 
   Size:        853     902
 
SphI G_CATG'C

Cuts at:      0    1267    1755 
   Size:       1267     488
 
StuI AGG'CCT

Cuts at:      0    1327    1755 
   Size:       1327     428
 
StyI C'CwwG_G

Cuts at:      0    1646    1740    1755 
   Size:       1646      94      15

  Fragments arranged by size: 

                1646      94      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1001    1033    1128    1160    1755 
   Size:       1001      32      95      32     595

  Fragments arranged by size: 

                1001     595      95      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1657    1755 
   Size:       1657      98
 
XmnI GAAnn'nnTTC

Cuts at:      0     373    1755 
   Size:        373    1382
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BfrBI     BglI    BglII    BmgBI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaXI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BssSI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     DraI     EaeI     EarI     EciI  Eco57MI
    EcoNI     FalI    HaeII    Hin4I   HincII  HindIII     KasI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI
     NspI     PciI    PflMI     PstI    PvuII    SacII     SapI     SfcI
     SfoI     SmlI     SphI     StuI     StyI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BciVI     BclI     BlpI
 Bme1580I     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I     ClaI   DraIII     DrdI
     EagI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     KpnI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII     TatI     XbaI
     XcmI     XhoI     XmaI     ZraI
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