Restriction map for pFN21AB0940
 (Circular) (Six-base) MAPSORT of: pFN21AB0940.seq  Check: 8464  from: 1  to: 5875



pFN21AB0940  5875 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:33  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2337    3685     278 
   Size:         53      83     186     936     801    1348    2468

  Fragments arranged by size: 

                2468    1348     936     801     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3218    1937 
   Size:       1281    4594
 
Acc65I G'GTAC_C

Cuts at:   3196    3196 
   Size:       5875
 
AclI AA'CG_TT

Cuts at:   1563    4002    4375    1563 
   Size:       2439     373    3063

  Fragments arranged by size: 

                3063    2439     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2928    3938    5322    5590    1632 
   Size:       1296    1010    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1296    1010     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5678
 
AflIII A'CryG_T

Cuts at:   1203    2712    4774    5751    1203 
   Size:       1509    2062     977    1327

  Fragments arranged by size: 

                2062    1509    1327     977
 
AgeI A'CCGG_T

Cuts at:   2517    4706    2517 
   Size:       2189    3686
 
AhdI GACnn_n'nnGTC

Cuts at:   4604    4604 
   Size:       5875
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5875
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5875
 
AlwNI CAG_nnn'CTG

Cuts at:   3618    5190    3618 
   Size:       1572    4303
 
ApaLI G'TGCA_C

Cuts at:   3932    5088    3932 
   Size:       1156    4719
 
ApoI r'AATT_y

Cuts at:   1493    3184    3410    3562    5671    1493 
   Size:       1691     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1691     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2976    4429     160 
   Size:        872    1944    1453    1606

  Fragments arranged by size: 

                1944    1606    1453     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5875
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3200    1870 
   Size:         73    1257    4545

  Fragments arranged by size: 

                4545    1257      73
 
BamHI G'GATC_C

Cuts at:   3205    3572    3205 
   Size:        367    5508
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3196    4651     618 
   Size:        325     217     627    1409    1455    1842

  Fragments arranged by size: 

                1842    1455    1409     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2198    3194    1391 
   Size:        807     996    4072

  Fragments arranged by size: 

                4072     996     807
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4904
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4066    4100    1903 
   Size:         34    2129      34    3678

  Fragments arranged by size: 

                3678    2129      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3767    4977    3767 
   Size:       1210    4665
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5875
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4486     136 
   Size:        107     122      71     631     605    2814    1525

  Fragments arranged by size: 

                2814    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5870    5870 
   Size:       5875
 
BlpI GC'TnA_GC

Cuts at:   3292    3292 
   Size:       5875
 
Bme1580I G_kGCm'C

Cuts at:   1790    3936    5092    1790 
   Size:       2146    1156    2573

  Fragments arranged by size: 

                2573    2146    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2715    1318 
   Size:       1397    4478
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3606    4564     442 
   Size:        416      53      83     399      24    2189     958    1753

  Fragments arranged by size: 

                2189    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5875
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4535    1239 
   Size:       3296    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4000    4865    5163    4000 
   Size:        865     298    4712

  Fragments arranged by size: 

                4712     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4538     882 
   Size:        617    3039    2219

  Fragments arranged by size: 

                3039    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3666     493 
   Size:        711    2462    2702

  Fragments arranged by size: 

                2702    2462     711
 
BsaBI GATnn'nnATC

Cuts at:   2587    5869    2587 
   Size:       3282    2593
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2334    3682    4064    4699 
   Size:         53      83     186     936     801    1348     382     635

Cuts at:   4699     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1348     936     801     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2517    4308    4706    4980    5127    5538    2517 
   Size:       1791     398     274     147     411    2854

  Fragments arranged by size: 

                2854    1791     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3063    3093    3146    3176    1178 
   Size:         30     165      30    1660      30      53      30    3877

  Fragments arranged by size: 

                3877    1660     165      53      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2124    2188    1186 
   Size:        938      64    4873

  Fragments arranged by size: 

                4873     938      64
 
BseYI C'CCAG_C

Cuts at:   2186    2777    5078    5574    2186 
   Size:        591    2301     496    2487

  Fragments arranged by size: 

                2487    2301     591     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2123    3017    1163 
   Size:        573     387     894    4021

  Fragments arranged by size: 

                4021     894     573     387
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3325    4086    5114    1540 
   Size:        275     186    1324     761    1028    2301

  Fragments arranged by size: 

                2301    1324    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3115    3936    4021    5092    3115 
   Size:        821      85    1071    3898

  Fragments arranged by size: 

                3898    1071     821      85
 
BsmI GAATG_Cn'

Cuts at:   3386    3479    3386 
   Size:         93    5782
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2198    3115    3194    3936    4021    5092    1391 
   Size:        399     408     917      79     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     917     742     408     399      85      79
            
 
BspEI T'CCGG_A

Cuts at:   5538    5538 
   Size:       5875
 
BspHI T'CATG_A

Cuts at:   2653    2862    3764    5494    2653 
   Size:        209     902    1730    3034

  Fragments arranged by size: 

                3034    1730     902     209
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3230     844 
   Size:       2386    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3762    1394 
   Size:        429    1939    3507

  Fragments arranged by size: 

                3507    1939     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4370    4544      65 
   Size:       1183    3122     174    1396

  Fragments arranged by size: 

                3122    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2107    2517    4519    4706    1700 
   Size:        110     297     410    2002     187    2869

  Fragments arranged by size: 

                2869    2002     410     297     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5875
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5875
 
BssSI C'ACGA_G

Cuts at:   3935    4947    3935 
   Size:       1012    4863
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5875
 
BstBI TT'CG_AA

Cuts at:   4679    4679 
   Size:       5875
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5875
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2179    2179 
   Size:       5875
 
BstYI r'GATC_y

Cuts at:   1972    3205    3572    3957    3974    5426    5512    5524    5870 
   Size:       1233     367     385      17    1452      86      12     346

Cuts at:   5870    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1233     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2125    2178    3066     513 
   Size:        552     939     121      53     888    3322

  Fragments arranged by size: 

                3322     939     888     552     121      53
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5136

  Fragments arranged by size: 

                5136     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2743    3167    3391    4185    4205    2743 
   Size:        424     224     794      20    4413

  Fragments arranged by size: 

                4413     794     424     224      20
 
ClaI AT'CG_AT

Cuts at:   1554    2553    1554 
   Size:        999    4876
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2812    2847     529 
   Size:         35    2248      35    3557

  Fragments arranged by size: 

                3557    2248      35      35
 
DraI TTT'AAA

Cuts at:   2217    3179    2217 
   Size:        962    4913
 
DraIII CAC_nnn'GTG

Cuts at:   2723    2723 
   Size:       5875
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4882     809 
   Size:        507    3566    1802

  Fragments arranged by size: 

                3566    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2707    3322    4211 
   Size:         54    1425      50     275      18     877     615     889

Cuts at:   4211    5840       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     877     615     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3322    1537 
   Size:        275    1510    4090

  Fragments arranged by size: 

                4090    1510     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2258    3805    1353 
   Size:        905    1547    3423

  Fragments arranged by size: 

                3423    1547     905
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4462    4836    4982     387 
   Size:       1064     138    2873     374     146    1280

  Fragments arranged by size: 

                2873    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2928    3938    4535    5322    5590    1239 
   Size:        393    1296    1010     597     787     268    1524

  Fragments arranged by size: 

                1524    1296    1010     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2080    2693    3119    2080 
   Size:        613     426    4836

  Fragments arranged by size: 

                4836     613     426
 
EcoRI G'AATT_C

Cuts at:   3184    3562    3184 
   Size:        378    5497
 
FspI TGC'GCA

Cuts at:   4381    4381 
   Size:       5875
 
HaeII r_GCGC'y

Cuts at:   1678    5022    1678 
   Size:       3344    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2043    2075    2235    2267    2324 
   Size:         32     135      32     471      32     160      32      57

Cuts at:   2324    2356    2621    2651    2653    2683    2934    2966    4516 
   Size:         32     265      30       2      30     251      32    1550

Cuts at:   4516    4548    4590    4622    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1550     471     265     251     160     135      57
                  42      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3219    3465     669 
   Size:        988     281    1281     246    3079

  Fragments arranged by size: 

                3079    1281     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5875
 
HpaI GTT'AAC

Cuts at:   3465    3465 
   Size:       5875
 
KpnI G_GTAC'C

Cuts at:   3200    3200 
   Size:       5875
 
MfeI C'AATT_G

Cuts at:   2965    3474    2965 
   Size:        509    5366
 
MluI A'CGCG_T

Cuts at:   5751    5751 
   Size:       5875
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3042    4989    5173    1305 
   Size:       1737    1947     184    2007

  Fragments arranged by size: 

                2007    1947    1737     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2709      10 
   Size:         54    1425     343     877    3176

  Fragments arranged by size: 

                3176    1425     877     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3143    3833    4192    4351     518 
   Size:        648    1977     690     359     159    2042

  Fragments arranged by size: 

                2042    1977     690     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2703    3291    3968    5116    5361    1606 
   Size:       1097     588     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1097     677     588     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2178    3066     513 
   Size:        552     939     174     888    3322

  Fragments arranged by size: 

                3322     939     888     552     174
 
NdeI CA'TA_TG

Cuts at:    387    3099     387 
   Size:       2712    3163
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5875
 
NotI GC'GGCC_GC

Cuts at:   3322    3322 
   Size:       5875
 
NspI r_CATG'y

Cuts at:   1132    1229    2090    3233    4778    1132 
   Size:         97     861    1143    1545    2229

  Fragments arranged by size: 

                2229    1545    1143     861      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5875
 
PciI A'CATG_T

Cuts at:   4774    4774 
   Size:       5875
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5875
 
PmeI GTTT'AAAC

Cuts at:   3179    3179 
   Size:       5875
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3944    3976    5457    5489    3944 
   Size:         32    1481      32    4330

  Fragments arranged by size: 

                4330    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2080    3119    2080 
   Size:       1039    4836
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5875
 
PsiI TTA'TAA

Cuts at:   3048    3445    3048 
   Size:        397    5478
 
PstI C_TGCA'G

Cuts at:    830    3227     830 
   Size:       2397    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5875
 
SalI G'TCGA_C

Cuts at:   1936    3217    1936 
   Size:       1281    4594
 
SanDI GG'GwC_CC

Cuts at:   2080    2080 
   Size:       5875
 
SbfI CC_TGCA'GG

Cuts at:   3227    3227 
   Size:       5875
 
ScaI AGT'ACT

Cuts at:   4123    5835    4123 
   Size:       1712    4163
 
SfcI C'TryA_G

Cuts at:    826    1045    3223    4358    5039    5230     826 
   Size:        219    2178    1135     681     191    1471

  Fragments arranged by size: 

                2178    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5875
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5875
 
SmaI CCC'GGG

Cuts at:   1872    3202    1872 
   Size:       1330    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3979    4880    5142     820 
   Size:        197     926    2036     901     262    1553

  Fragments arranged by size: 

                2036    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3666     493 
   Size:       3173    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5875
 
SphI G_CATG'C

Cuts at:   1132    2090    3233    1132 
   Size:        958    1143    3774

  Fragments arranged by size: 

                3774    1143     958
 
SspI AAT'ATT

Cuts at:      5      52    3799       5 
   Size:         47    3747    2081

  Fragments arranged by size: 

                3747    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5875
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2178    3066    3314     513 
   Size:        552     939     174     888     248    3074

  Fragments arranged by size: 

                3074     939     888     552     248     174
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3902    3919    4072    4257    4702     325 
   Size:       1255    2322      17     153     185     445    1498

  Fragments arranged by size: 

                2322    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4121    5833      96 
   Size:        274      80      33      51    1443    2144    1712     138

  Fragments arranged by size: 

                2144    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3146    3178    5835    5867    3146 
   Size:         32    2657      32    3154

  Fragments arranged by size: 

                3154    2657      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5875
 
XbaI T'CTAG_A

Cuts at:   3211    3211 
   Size:       5875
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5875
 
XmaI C'CCGG_G

Cuts at:   1870    3200    1870 
   Size:       1330    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4004    4004 
   Size:       5875
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2335    3683     276 
   Size:         53      83     186     936     801    1348    2468

  Fragments arranged by size: 

                2468    1348     936     801     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI     SalI
    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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