(Circular) (Six-base) MAPSORT of: pFN21AB0940.seq Check: 8464 from: 1 to: 5875 pFN21AB0940 5875 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2337 3685 278 Size: 53 83 186 936 801 1348 2468 Fragments arranged by size: 2468 1348 936 801 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2928 3938 5322 5590 1632 Size: 1296 1010 1384 268 1917 Fragments arranged by size: 1917 1384 1296 1010 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 2712 4774 5751 1203 Size: 1509 2062 977 1327 Fragments arranged by size: 2062 1509 1327 977 AgeI A'CCGG_T Cuts at: 2517 4706 2517 Size: 2189 3686 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 2976 4429 160 Size: 872 1944 1453 1606 Fragments arranged by size: 1944 1606 1453 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3196 4651 618 Size: 325 217 627 1409 1455 1842 Fragments arranged by size: 1842 1455 1409 627 325 217 BanII G_rGCy'C Cuts at: 1391 2198 3194 1391 Size: 807 996 4072 Fragments arranged by size: 4072 996 807 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4904 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 3936 5092 1790 Size: 2146 1156 2573 Fragments arranged by size: 2573 2146 1156 BmgBI CAC'GTC Cuts at: 1318 2715 1318 Size: 1397 4478 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4535 1239 Size: 3296 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4000 4865 5163 4000 Size: 865 298 4712 Fragments arranged by size: 4712 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 2587 5869 2587 Size: 3282 2593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2334 3682 4064 4699 Size: 53 83 186 936 801 1348 382 635 Cuts at: 4699 275 Size: 1451 Fragments arranged by size: 1451 1348 936 801 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2517 4308 4706 4980 5127 5538 2517 Size: 1791 398 274 147 411 2854 Fragments arranged by size: 2854 1791 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3063 3093 3146 3176 1178 Size: 30 165 30 1660 30 53 30 3877 Fragments arranged by size: 3877 1660 165 53 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2124 2188 1186 Size: 938 64 4873 Fragments arranged by size: 4873 938 64 BseYI C'CCAG_C Cuts at: 2186 2777 5078 5574 2186 Size: 591 2301 496 2487 Fragments arranged by size: 2487 2301 591 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 3017 1163 Size: 573 387 894 4021 Fragments arranged by size: 4021 894 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3115 3936 4021 5092 3115 Size: 821 85 1071 3898 Fragments arranged by size: 3898 1071 821 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 3115 3194 3936 4021 5092 1391 Size: 399 408 917 79 742 85 1071 2174 Fragments arranged by size: 2174 1071 917 742 408 399 85 79 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 2653 2862 3764 5494 2653 Size: 209 902 1730 3034 Fragments arranged by size: 3034 1730 902 209 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2517 4519 4706 1700 Size: 110 297 410 2002 187 2869 Fragments arranged by size: 2869 2002 410 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2179 Size: 5875 BstYI r'GATC_y Cuts at: 1972 3205 3572 3957 3974 5426 5512 5524 5870 Size: 1233 367 385 17 1452 86 12 346 Cuts at: 5870 1972 Size: 1977 Fragments arranged by size: 1977 1452 1233 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2125 2178 3066 513 Size: 552 939 121 53 888 3322 Fragments arranged by size: 3322 939 888 552 121 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2743 3167 3391 4185 4205 2743 Size: 424 224 794 20 4413 Fragments arranged by size: 4413 794 424 224 20 ClaI AT'CG_AT Cuts at: 1554 2553 1554 Size: 999 4876 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2812 2847 529 Size: 35 2248 35 3557 Fragments arranged by size: 3557 2248 35 35 DraI TTT'AAA Cuts at: 2217 3179 2217 Size: 962 4913 DraIII CAC_nnn'GTG Cuts at: 2723 2723 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2707 3322 4211 Size: 54 1425 50 275 18 877 615 889 Cuts at: 4211 5840 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 877 615 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 3805 1353 Size: 905 1547 3423 Fragments arranged by size: 3423 1547 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2928 3938 4535 5322 5590 1239 Size: 393 1296 1010 597 787 268 1524 Fragments arranged by size: 1524 1296 1010 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2080 2693 3119 2080 Size: 613 426 4836 Fragments arranged by size: 4836 613 426 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 5022 1678 Size: 3344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2075 2235 2267 2324 Size: 32 135 32 471 32 160 32 57 Cuts at: 2324 2356 2621 2651 2653 2683 2934 2966 4516 Size: 32 265 30 2 30 251 32 1550 Cuts at: 4516 4548 4590 4622 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1550 471 265 251 160 135 57 42 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 2965 3474 2965 Size: 509 5366 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 4989 5173 1305 Size: 1737 1947 184 2007 Fragments arranged by size: 2007 1947 1737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2709 10 Size: 54 1425 343 877 3176 Fragments arranged by size: 3176 1425 877 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3143 3833 4192 4351 518 Size: 648 1977 690 359 159 2042 Fragments arranged by size: 2042 1977 690 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2703 3291 3968 5116 5361 1606 Size: 1097 588 677 1148 245 2120 Fragments arranged by size: 2120 1148 1097 677 588 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2178 3066 513 Size: 552 939 174 888 3322 Fragments arranged by size: 3322 939 888 552 174 NdeI CA'TA_TG Cuts at: 387 3099 387 Size: 2712 3163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2090 3233 4778 1132 Size: 97 861 1143 1545 2229 Fragments arranged by size: 2229 1545 1143 861 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 3119 2080 Size: 1039 4836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3048 3445 3048 Size: 397 5478 PstI C_TGCA'G Cuts at: 830 3227 830 Size: 2397 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SanDI GG'GwC_CC Cuts at: 2080 2080 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 3223 4358 5039 5230 826 Size: 219 2178 1135 681 191 1471 Fragments arranged by size: 2178 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3979 4880 5142 820 Size: 197 926 2036 901 262 1553 Fragments arranged by size: 2036 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 2090 3233 1132 Size: 958 1143 3774 Fragments arranged by size: 3774 1143 958 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 3066 3314 513 Size: 552 939 174 888 248 3074 Fragments arranged by size: 3074 939 888 552 248 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3902 3919 4072 4257 4702 325 Size: 1255 2322 17 153 185 445 1498 Fragments arranged by size: 2322 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3146 3178 5835 5867 3146 Size: 32 2657 32 3154 Fragments arranged by size: 3154 2657 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2335 3683 276 Size: 53 83 186 936 801 1348 2468 Fragments arranged by size: 2468 1348 936 801 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI