Restriction map for
pFN21AB0926
(Circular) (Six-base) MAPSORT of: pFN21AB0926.seq Check: 6351 from: 1 to: 5773
pFN21AB0926 5773 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2355 2355
Size: 5773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2419 2923 2999 3032 3077 3836 5220 5488
Size: 787 504 76 33 45 759 1384 268
Cuts at: 5488 1632
Size: 1917
Fragments arranged by size:
1917 1384 787 759 504 268 76 45
33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2160 2984 1184
Size: 976 824 3973
Fragments arranged by size:
3973 976 824
AlwNI CAG_nnn'CTG
Cuts at: 2269 2347 2460 2917 3516 5088 2269
Size: 78 113 457 599 1572 2954
Fragments arranged by size:
2954 1572 599 457 113 78
ApaI G_GGCC'C
Cuts at: 2154 2202 2154
Size: 48 5725
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2201 3098 1870
Size: 73 258 897 4545
Fragments arranged by size:
4545 897 258 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 3094 4549 618
Size: 325 217 627 947 360 1455 1842
Fragments arranged by size:
1842 1455 947 627 360 325 217
BanII G_rGCy'C
Cuts at: 1391 2049 2154 2202 2609 2937 3092 1391
Size: 658 105 48 407 328 155 4072
Fragments arranged by size:
4072 658 407 328 155 105 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 2709 928
Size: 971 367 443 3992
Fragments arranged by size:
3992 971 443 367
BbvCI CC'TCA_GC
Cuts at: 2054 2744 2054
Size: 690 5083
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 2206 4384
Size: 107 122 71 631 605 433 101 2178
Cuts at: 4384 136
Size: 1525
Fragments arranged by size:
2178 1525 631 605 433 122 107 101
71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2154 2202 3834 4990 1790
Size: 364 48 1632 1156 2573
Fragments arranged by size:
2573 1632 1156 364 48
BmgBI CAC'GTC
Cuts at: 1318 2881 1318
Size: 1563 4210
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2673 2736 2743 4433 1239
Size: 941 493 63 7 1690 2579
Fragments arranged by size:
2579 1690 941 493 63 7
Bpu10I CC'TnA_GC
Cuts at: 2014 2054 2744 2014
Size: 40 690 5043
Fragments arranged by size:
5043 690 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2791 3898 4763 5061 2791
Size: 1107 865 298 3503
Fragments arranged by size:
3503 1107 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2929 3564 493
Size: 711 1725 635 2702
Fragments arranged by size:
2702 1725 711 635
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2901 3580 3962 4597
Size: 53 83 186 936 1368 679 382 635
Cuts at: 4597 275
Size: 1451
Fragments arranged by size:
1451 1368 936 679 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 2591 2606 2621
Size: 30 165 30 639 30 519 15 15
Cuts at: 2621 2636 1178
Size: 15 4315
Fragments arranged by size:
4315 639 519 165 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2611 2614 1186
Size: 1425 3 4345
Fragments arranged by size:
4345 1425 3
BseYI C'CCAG_C
Cuts at: 2087 3016 4976 5472 2087
Size: 929 1960 496 2388
Fragments arranged by size:
2388 1960 929 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 1163
Size: 573 459 4741
Fragments arranged by size:
4741 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2937 3834 3919 4990 2937
Size: 897 85 1071 3720
Fragments arranged by size:
3720 1071 897 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2154 2202 2609 2937 3092 3834
Size: 399 259 105 48 407 328 155 742
Cuts at: 3834 3919 4990 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 407 399 328 259 155
105 85 48
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 2994 3662 5392 2994
Size: 668 1730 3375
Fragments arranged by size:
3375 1730 668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 3128 844
Size: 1511 773 3489
Fragments arranged by size:
3489 1511 773
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 3660 1394
Size: 429 383 1454 3507
Fragments arranged by size:
3507 1454 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4417 4604 1700
Size: 110 2607 187 2869
Fragments arranged by size:
2869 2607 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 2948 3833 4845 2948
Size: 885 1012 3876
Fragments arranged by size:
3876 1012 885
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2250 794
Size: 1456 4317
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2067 2067
Size: 5773
BtgI C'CryG_G
Cuts at: 513 1065 2030 2079 2843 513
Size: 552 965 49 764 3443
Fragments arranged by size:
3443 965 764 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2439 487
Size: 683 56 1213 3821
Fragments arranged by size:
3821 1213 683 56
BtsI GCAGTG_nn'
Cuts at: 2470 3289 4083 4103 2470
Size: 819 794 20 4140
Fragments arranged by size:
4140 819 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2840 3220
Size: 54 1425 50 275 18 377 633 380
Cuts at: 3220 4109 5738 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 633 380 377 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2438 2526 2942 2975 3703 1353
Size: 1085 88 416 33 728 3423
Fragments arranged by size:
3423 1085 728 416 88 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2572 2851 4360 4734 4880 387
Size: 1064 138 983 279 1509 374 146 1280
Fragments arranged by size:
1509 1280 1064 983 374 279 146 138
EcoICRI GAG'CTC
Cuts at: 2935 2935
Size: 5773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2419 2673 2736 2743 2923 2999
Size: 393 548 239 254 63 7 180 76
Cuts at: 2999 3032 3077 3836 4433 5220 5488 1239
Size: 33 45 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 597 548 393 268 254
239 180 76 63 45 33 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2452 2452
Size: 5773
EcoO109I rG'GnC_Cy
Cuts at: 2150 2151 2921 3020 2150
Size: 1 770 99 4903
Fragments arranged by size:
4903 770 99 1
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 5773
FspI TGC'GCA
Cuts at: 2826 4279 2826
Size: 1453 4320
HaeII r_GCGC'y
Cuts at: 1678 4920 1678
Size: 3242 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2195 2227 2297
Size: 32 135 32 470 32 121 32 70
Cuts at: 2297 2329 4414 4446 4488 4520 1373
Size: 32 2085 32 42 32 2626
Fragments arranged by size:
2626 2085 470 135 121 70 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4887 5071 1305
Size: 3582 184 2007
Fragments arranged by size:
3582 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3951
Fragments arranged by size:
3951 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2993 3731 4090 4249 518
Size: 648 1827 738 359 159 2042
Fragments arranged by size:
2042 1827 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2690 3189 3866 5014 5259 1606
Size: 426 658 499 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 658 499 426 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2454 1348
Size: 1106 4667
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PfoI T'CCnGG_A
Cuts at: 2184 2481 2184
Size: 297 5476
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PmlI CAC'GTG
Cuts at: 2929 2929
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3020 3020
Size: 5773
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspOMI G'GGCC_C
Cuts at: 2150 2198 2150
Size: 48 5725
PstI C_TGCA'G
Cuts at: 830 2226 2465 3125 830
Size: 1396 239 660 3478
Fragments arranged by size:
3478 1396 660 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
SacI G_AGCT'C
Cuts at: 2937 2937
Size: 5773
SacII CC_GC'GG
Cuts at: 2033 2033
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SanDI GG'GwC_CC
Cuts at: 3020 3020
Size: 5773
SapI GCTCTTCn'nnn_
Cuts at: 2942 2942
Size: 5773
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SexAI A'CCwGG_T
Cuts at: 2538 2538
Size: 5773
SfcI C'TryA_G
Cuts at: 826 1045 2222 2461 2566 3121 4256 4937 5128
Size: 219 1177 239 105 555 1135 681 191
Cuts at: 5128 826
Size: 1471
Fragments arranged by size:
1471 1177 1135 681 555 239 219 191
105
SfiI GGCCn_nnn'nGGCC
Cuts at: 2206 2206
Size: 5773
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3100 1872
Size: 1228 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2806 3877 4778 5040 820
Size: 197 926 863 1071 901 262 1553
Fragments arranged by size:
1553 1071 926 901 863 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 2749 1517
Size: 1232 4541
StyI C'CwwG_G
Cuts at: 513 1065 2665 3212 513
Size: 552 1600 547 3074
Fragments arranged by size:
3074 1600 552 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 3817 3970 4155 4600 325
Size: 1255 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2631 4019 5731
Size: 274 80 33 51 1443 654 1388 1712
Cuts at: 5731 96
Size: 138
Fragments arranged by size:
1712 1443 1388 654 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2606 2623 2638 2870 2902 5733 5765 2591
Size: 15 17 15 232 32 2831 32 2599
Fragments arranged by size:
2831 2599 232 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 2990 1316
Size: 1674 4099
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2669 2669
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3098 1870
Size: 1228 4545
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PsrI PvuII RsrII SfoI SrfI SwaI