(Circular) (Six-base) MAPSORT of: pFN21AB0912.seq Check: 4233 from: 1 to: 5656 pFN21AB0912 5656 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2424 2424 Size: 5656 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2471 3466 278 Size: 53 83 186 936 935 995 2468 Fragments arranged by size: 2468 995 936 935 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2549 2606 3719 5103 5371 1632 Size: 917 57 1113 1384 268 1917 Fragments arranged by size: 1917 1384 1113 917 268 57 AfeI AGC'GCT Cuts at: 2696 2696 Size: 5656 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2159 2226 4555 5532 1203 Size: 956 67 2329 977 1327 Fragments arranged by size: 2329 1327 977 956 67 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2319 2572 2779 3399 4971 2319 Size: 253 207 620 1572 3004 Fragments arranged by size: 3004 1572 620 253 207 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2981 1870 Size: 73 1038 4545 Fragments arranged by size: 4545 1038 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 2977 4432 618 Size: 325 217 627 308 882 1455 1842 Fragments arranged by size: 1842 1455 882 627 325 308 217 BanII G_rGCy'C Cuts at: 1391 2975 1391 Size: 1584 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2471 928 Size: 971 572 4113 Fragments arranged by size: 4113 971 572 BbvCI CC'TCA_GC Cuts at: 2312 2312 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2047 5651 2047 Size: 3604 2052 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 3717 4873 1790 Size: 1927 1156 2573 Fragments arranged by size: 2573 1927 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2131 2198 3387 Size: 416 53 83 399 24 714 67 1189 Cuts at: 3387 4345 442 Size: 958 1753 Fragments arranged by size: 1753 1189 958 714 416 399 83 67 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 2480 2594 2663 4316 1239 Size: 1151 90 114 69 1653 2579 Fragments arranged by size: 2579 1653 1151 114 90 69 Bpu10I CC'TnA_GC Cuts at: 2312 2312 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2830 3447 493 Size: 711 1626 617 2702 Fragments arranged by size: 2702 1626 711 617 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2468 3463 3845 4480 Size: 53 83 186 936 935 995 382 635 Cuts at: 4480 275 Size: 1451 Fragments arranged by size: 1451 995 936 935 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2453 2483 1178 Size: 30 165 30 1050 30 4351 Fragments arranged by size: 4351 1050 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2591 1186 Size: 910 495 4251 Fragments arranged by size: 4251 910 495 BseYI C'CCAG_C Cuts at: 2643 4859 5355 2643 Size: 2216 496 2944 Fragments arranged by size: 2944 2216 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5083 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2190 3106 3867 4895 1540 Size: 275 186 189 916 761 1028 2301 Fragments arranged by size: 2301 1028 916 761 275 189 186 BsiHKAI G_wGCw'C Cuts at: 2328 2679 3717 3802 4873 2328 Size: 351 1038 85 1071 3111 Fragments arranged by size: 3111 1071 1038 351 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2679 2975 3717 3802 4873 1391 Size: 399 538 351 296 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 351 296 85 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 2925 3545 5275 2925 Size: 620 1730 3306 Fragments arranged by size: 3306 1730 620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2424 3011 844 Size: 1580 587 3489 Fragments arranged by size: 3489 1580 587 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 2756 3716 4728 2756 Size: 960 1012 3684 Fragments arranged by size: 3684 1012 960 BstAPI GCAn_nnn'nTGC Cuts at: 794 2127 794 Size: 1333 4323 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2047 2598 2986 3353 3738 3755 5207 5293 Size: 75 551 388 367 385 17 1452 86 Cuts at: 5293 5305 5651 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 551 388 385 367 346 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2434 487 Size: 683 56 1208 3709 Fragments arranged by size: 3709 1208 683 56 BtsI GCAGTG_nn' Cuts at: 2074 2554 3172 3966 3986 2074 Size: 480 618 794 20 3744 Fragments arranged by size: 3744 794 618 480 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DraIII CAC_nnn'GTG Cuts at: 2139 2139 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2781 2832 3103 Size: 54 1425 50 275 18 951 51 271 Cuts at: 3103 3992 5621 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 951 889 275 271 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2943 3586 1353 Size: 1590 643 3423 Fragments arranged by size: 3423 1590 643 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2510 4243 4617 4763 387 Size: 1064 138 921 1733 374 146 1280 Fragments arranged by size: 1733 1280 1064 921 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2480 2549 2594 2606 2663 3719 Size: 393 758 90 69 45 12 57 1056 Cuts at: 3719 4316 5103 5371 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1056 787 758 597 393 268 90 69 57 45 12 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 2937 4162 2937 Size: 1225 4431 HaeII r_GCGC'y Cuts at: 1678 2698 4803 1678 Size: 1020 2105 2531 Fragments arranged by size: 2531 2105 1020 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 2640 2647 2672 Size: 32 135 32 881 32 155 7 25 Cuts at: 2672 2679 2701 2733 4297 4329 4371 4403 1373 Size: 7 22 32 1564 32 42 32 2626 Fragments arranged by size: 2626 1564 881 155 135 42 32 32 32 32 32 32 25 22 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2041 2554 2734 3000 3246 669 Size: 988 281 103 513 180 266 246 3079 Fragments arranged by size: 3079 988 513 281 266 246 180 103 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 2583 3255 2583 Size: 672 4984 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2783 2834 10 Size: 54 1425 343 951 51 2832 Fragments arranged by size: 2832 1425 951 343 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 3614 3973 4132 518 Size: 648 2448 359 159 2042 Fragments arranged by size: 2448 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2474 2491 2518 2643 3072 3749 4897 5142 Size: 868 17 27 125 429 677 1148 245 Cuts at: 5142 1606 Size: 2120 Fragments arranged by size: 2120 1148 868 677 429 245 125 27 17 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2114 2163 2230 2536 3014 4559 1132 Size: 97 885 49 67 306 478 1545 2229 Fragments arranged by size: 2229 1545 885 478 306 97 67 49 PasI CC'CwG_GG Cuts at: 1348 2773 1348 Size: 1425 4231 PciI A'CATG_T Cuts at: 2159 2226 4555 2159 Size: 67 2329 3260 Fragments arranged by size: 3260 2329 67 PflMI CCAn_nnn'nTGG Cuts at: 1108 2779 2890 1108 Size: 1671 111 3874 Fragments arranged by size: 3874 1671 111 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2798 2830 2798 Size: 32 5624 PstI C_TGCA'G Cuts at: 830 2244 3008 830 Size: 1414 764 3478 Fragments arranged by size: 3478 1414 764 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2474 2491 2518 2474 Size: 17 27 5612 Fragments arranged by size: 5612 27 17 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2240 2272 3004 4139 4820 5011 826 Size: 219 1195 32 732 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 732 681 219 191 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3760 4661 4923 820 Size: 197 926 1817 901 262 1553 Fragments arranged by size: 1817 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 2919 1517 Size: 1402 4254 StyI C'CwwG_G Cuts at: 513 1065 2004 3095 513 Size: 552 939 1091 3074 Fragments arranged by size: 3074 1091 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2150 2599 3683 3700 3853 4038 4483 Size: 1255 570 449 1084 17 153 185 445 Cuts at: 4483 325 Size: 1498 Fragments arranged by size: 1498 1255 1084 570 449 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2089 3902 5614 Size: 274 80 33 51 1443 112 1813 1712 Cuts at: 5614 96 Size: 138 Fragments arranged by size: 1813 1712 1443 274 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2469 3464 276 Size: 53 83 186 936 935 995 2468 Fragments arranged by size: 2468 995 936 935 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI